(Circular) (Six-base) MAPSORT of: pFN21AE9505.seq Check: 6580 from: 1 to: 5716 pFN21AE9505 5716 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2449 2763 2449 Size: 314 5402 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3526 278 Size: 53 83 186 936 1990 2468 Fragments arranged by size: 2468 1990 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2432 3059 1937 Size: 495 627 4594 Fragments arranged by size: 4594 627 495 Acc65I G'GTAC_C Cuts at: 3037 3037 Size: 5716 AclI AA'CG_TT Cuts at: 1563 3843 4216 1563 Size: 2280 373 3063 Fragments arranged by size: 3063 2280 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3779 5163 5431 1632 Size: 2147 1384 268 1917 Fragments arranged by size: 2147 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5519 AflIII A'CryG_T Cuts at: 1203 4615 5592 1203 Size: 3412 977 1327 Fragments arranged by size: 3412 1327 977 AgeI A'CCGG_T Cuts at: 4547 4547 Size: 5716 AhdI GACnn_n'nnGTC Cuts at: 4445 4445 Size: 5716 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5716 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5716 AlwNI CAG_nnn'CTG Cuts at: 3459 5031 3459 Size: 1572 4144 ApaI G_GGCC'C Cuts at: 2640 2812 2935 2640 Size: 172 123 5421 Fragments arranged by size: 5421 172 123 ApaLI G'TGCA_C Cuts at: 3773 4929 3773 Size: 1156 4560 ApoI r'AATT_y Cuts at: 1493 3025 3251 3403 5512 1493 Size: 1532 226 152 2109 1697 Fragments arranged by size: 2109 1697 1532 226 152 AseI AT'TA_AT Cuts at: 160 1032 4270 160 Size: 872 3238 1606 Fragments arranged by size: 3238 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5716 AvaI C'yCGr_G Cuts at: 1870 1943 2414 3041 1870 Size: 73 471 627 4545 Fragments arranged by size: 4545 627 471 73 BamHI G'GATC_C Cuts at: 3046 3413 3046 Size: 367 5349 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2081 2152 2210 2743 3037 Size: 325 217 627 294 71 58 533 294 Cuts at: 3037 4492 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 533 325 294 294 217 71 58 BanII G_rGCy'C Cuts at: 1391 2640 2812 2896 2935 3035 1391 Size: 1249 172 84 39 100 4072 Fragments arranged by size: 4072 1249 172 100 84 39 BbeI G_GCGC'C Cuts at: 2085 2085 Size: 5716 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4745 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3907 3941 1903 Size: 34 1970 34 3678 Fragments arranged by size: 3678 1970 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3608 4818 3608 Size: 1210 4506 BclI T'GATC_A Cuts at: 1548 1548 Size: 5716 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2079 2090 2171 Size: 107 122 71 631 605 407 11 81 Cuts at: 2171 2180 2471 2816 4327 136 Size: 9 291 345 1511 1525 Fragments arranged by size: 1525 1511 631 605 407 345 291 122 107 81 71 11 9 BglII A'GATC_T Cuts at: 5711 5711 Size: 5716 BlpI GC'TnA_GC Cuts at: 3133 3133 Size: 5716 Bme1580I G_kGCm'C Cuts at: 1790 2283 2508 2640 2671 2711 2746 2812 2935 Size: 493 225 132 31 40 35 66 123 Cuts at: 2935 3777 4933 1790 Size: 842 1156 2573 Fragments arranged by size: 2573 1156 842 493 225 132 123 66 40 35 31 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5716 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2938 3447 4405 Size: 416 53 83 399 24 1521 509 958 Cuts at: 4405 442 Size: 1753 Fragments arranged by size: 1753 1521 958 509 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5716 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2569 4376 1239 Size: 1330 1807 2579 Fragments arranged by size: 2579 1807 1330 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3841 4706 5004 3841 Size: 865 298 4553 Fragments arranged by size: 4553 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3004 4379 882 Size: 617 1505 1375 2219 Fragments arranged by size: 2219 1505 1375 617 BsaAI yAC'GTr Cuts at: 493 1204 3507 493 Size: 711 2303 2702 Fragments arranged by size: 2702 2303 711 BsaBI GATnn'nnATC Cuts at: 5710 5710 Size: 5716 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2082 3523 3905 4540 Size: 53 83 186 936 549 1441 382 635 Cuts at: 4540 275 Size: 1451 Fragments arranged by size: 1451 1441 936 635 549 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2613 4149 4547 4821 4968 5379 2613 Size: 1536 398 274 147 411 2950 Fragments arranged by size: 2950 1536 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2194 2224 2564 2594 1178 Size: 30 165 30 791 30 340 30 4300 Fragments arranged by size: 4300 791 340 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2572 1186 Size: 1386 4330 BseYI C'CCAG_C Cuts at: 2183 2545 2679 2704 2739 2758 4919 5415 2183 Size: 362 134 25 35 19 2161 496 2484 Fragments arranged by size: 2484 2161 496 362 134 35 25 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2334 2477 2513 1163 Size: 573 598 143 36 4366 Fragments arranged by size: 4366 598 573 143 36 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2702 3166 3927 4955 1540 Size: 275 186 701 464 761 1028 2301 Fragments arranged by size: 2301 1028 761 701 464 275 186 BsiHKAI G_wGCw'C Cuts at: 2541 2772 3777 3862 4933 2541 Size: 231 1005 85 1071 3324 Fragments arranged by size: 3324 1071 1005 231 85 BsmI GAATG_Cn' Cuts at: 2602 3227 3320 2602 Size: 625 93 4998 Fragments arranged by size: 4998 625 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2283 2508 2541 2640 2671 2711 2746 Size: 399 493 225 33 99 31 40 35 Cuts at: 2746 2772 2812 2896 2935 3035 3777 3862 4933 Size: 26 40 84 39 100 742 85 1071 Cuts at: 4933 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 493 399 225 100 99 85 84 40 40 39 35 33 31 26 BspEI T'CCGG_A Cuts at: 5379 5379 Size: 5716 BspHI T'CATG_A Cuts at: 3605 5335 3605 Size: 1730 3986 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2449 2763 3071 844 Size: 1605 314 308 3489 Fragments arranged by size: 3489 1605 314 308 BsrBI CCG'CTC Cuts at: 1394 1823 2119 3603 1394 Size: 429 296 1484 3507 Fragments arranged by size: 3507 1484 429 296 BsrDI GCAATG_nn' Cuts at: 65 1248 2167 2283 4211 4385 65 Size: 1183 919 116 1928 174 1396 Fragments arranged by size: 1928 1396 1183 919 174 116 BsrFI r'CCGG_y Cuts at: 1700 1810 2523 2697 2904 4360 4547 1700 Size: 110 713 174 207 1456 187 2869 Fragments arranged by size: 2869 1456 713 207 187 174 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5716 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5716 BssSI C'ACGA_G Cuts at: 3776 4788 3776 Size: 1012 4704 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5716 BstBI TT'CG_AA Cuts at: 4520 4520 Size: 5716 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5716 BstXI CCAn_nnnn'nTGG Cuts at: 2143 2722 2143 Size: 579 5137 BstYI r'GATC_y Cuts at: 1972 3046 3413 3798 3815 5267 5353 5365 5711 Size: 1074 367 385 17 1452 86 12 346 Cuts at: 5711 1972 Size: 1977 Fragments arranged by size: 1977 1452 1074 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2936 2936 Size: 5716 BtgI C'CryG_G Cuts at: 513 1065 2004 2166 2235 2290 2649 513 Size: 552 939 162 69 55 359 3580 Fragments arranged by size: 3580 939 552 359 162 69 55 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4977 Fragments arranged by size: 4977 683 56 BtsI GCAGTG_nn' Cuts at: 2283 2322 2564 2909 3232 4026 4046 2283 Size: 39 242 345 323 794 20 3953 Fragments arranged by size: 3953 794 345 323 242 39 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5716 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5681 DraI TTT'AAA Cuts at: 3020 3020 Size: 5716 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4723 809 Size: 507 3407 1802 Fragments arranged by size: 3407 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2091 2232 2303 Size: 54 1425 50 275 18 261 141 71 Cuts at: 2303 2646 2652 2699 2802 3163 4052 5681 8 Size: 343 6 47 103 361 889 1629 43 Fragments arranged by size: 1629 1425 889 361 343 275 261 141 103 71 54 50 47 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 2699 3163 1537 Size: 275 887 464 4090 Fragments arranged by size: 4090 887 464 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2879 3646 1353 Size: 1526 767 3423 Fragments arranged by size: 3423 1526 767 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2255 2500 2891 4303 4677 4823 Size: 1064 138 666 245 391 1412 374 146 Cuts at: 4823 387 Size: 1280 Fragments arranged by size: 1412 1280 1064 666 391 374 245 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2569 3779 4376 5163 5431 1239 Size: 393 937 1210 597 787 268 1524 Fragments arranged by size: 1524 1210 937 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2636 2637 2808 2809 2932 2636 Size: 1 171 1 123 5420 Fragments arranged by size: 5420 171 123 1 1 EcoRI G'AATT_C Cuts at: 3025 3403 3025 Size: 378 5338 FspI TGC'GCA Cuts at: 4222 4222 Size: 5716 HaeII r_GCGC'y Cuts at: 1678 2085 2333 4863 1678 Size: 407 248 2530 2531 Fragments arranged by size: 2531 2530 407 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2984 3016 4357 4389 4431 Size: 32 135 32 1412 32 1341 32 42 Cuts at: 4431 4463 1373 Size: 32 2626 Fragments arranged by size: 2626 1412 1341 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3060 3306 669 Size: 988 281 1122 246 3079 Fragments arranged by size: 3079 1122 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5716 HpaI GTT'AAC Cuts at: 3306 3306 Size: 5716 KasI G'GCGC_C Cuts at: 2081 2081 Size: 5716 KpnI G_GTAC'C Cuts at: 3041 3041 Size: 5716 MfeI C'AATT_G Cuts at: 3315 3315 Size: 5716 MluI A'CGCG_T Cuts at: 5592 5592 Size: 5716 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4830 5014 1305 Size: 3525 184 2007 Fragments arranged by size: 3525 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2093 2654 2804 10 Size: 54 1425 343 261 561 150 2922 Fragments arranged by size: 2922 1425 561 343 261 150 54 MslI CAynn'nnrTG Cuts at: 518 1166 2720 3674 4033 4192 518 Size: 648 1554 954 359 159 2042 Fragments arranged by size: 2042 1554 954 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2104 2443 2658 2704 2856 2918 3132 3809 Size: 498 339 215 46 152 62 214 677 Cuts at: 3809 4957 5202 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 498 339 245 215 214 152 62 46 NaeI GCC'GGC Cuts at: 2525 2699 2525 Size: 174 5542 NarI GG'CG_CC Cuts at: 2082 2082 Size: 5716 NcoI C'CATG_G Cuts at: 513 1065 2004 2166 513 Size: 552 939 162 4063 Fragments arranged by size: 4063 939 552 162 NdeI CA'TA_TG Cuts at: 387 387 Size: 5716 NgoMIV G'CCGG_C Cuts at: 2523 2697 2523 Size: 174 5542 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5716 NotI GC'GGCC_GC Cuts at: 3163 3163 Size: 5716 NspI r_CATG'y Cuts at: 1132 1229 3074 4619 1132 Size: 97 1845 1545 2229 Fragments arranged by size: 2229 1845 1545 97 PasI CC'CwG_GG Cuts at: 1348 2947 1348 Size: 1599 4117 PciI A'CATG_T Cuts at: 4615 4615 Size: 5716 PflMI CCAn_nnn'nTGG Cuts at: 1108 2758 2995 1108 Size: 1650 237 3829 Fragments arranged by size: 3829 1650 237 PmeI GTTT'AAAC Cuts at: 3020 3020 Size: 5716 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3785 3817 5298 5330 3785 Size: 32 1481 32 4171 Fragments arranged by size: 4171 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5716 PsiI TTA'TAA Cuts at: 3286 3286 Size: 5716 PspOMI G'GGCC_C Cuts at: 2636 2808 2931 2636 Size: 172 123 5421 Fragments arranged by size: 5421 172 123 PstI C_TGCA'G Cuts at: 830 2559 3068 830 Size: 1729 509 3478 Fragments arranged by size: 3478 1729 509 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5716 PvuII CAG'CTG Cuts at: 2658 2856 2918 2658 Size: 198 62 5456 Fragments arranged by size: 5456 198 62 RsrII CG'GwC_CG Cuts at: 2056 2056 Size: 5716 SalI G'TCGA_C Cuts at: 1936 3058 1936 Size: 1122 4594 SapI GCTCTTCn'nnn_ Cuts at: 2879 2879 Size: 5716 SbfI CC_TGCA'GG Cuts at: 3068 3068 Size: 5716 ScaI AGT'ACT Cuts at: 3964 5676 3964 Size: 1712 4004 SexAI A'CCwGG_T Cuts at: 2216 2994 2216 Size: 778 4938 SfcI C'TryA_G Cuts at: 826 1045 2555 3064 4199 4880 5071 826 Size: 219 1510 509 1135 681 191 1471 Fragments arranged by size: 1510 1471 1135 681 509 219 191 SfoI GGC'GCC Cuts at: 2083 2083 Size: 5716 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5716 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5716 SmaI CCC'GGG Cuts at: 1872 3043 1872 Size: 1171 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3820 4721 4983 820 Size: 197 926 1877 901 262 1553 Fragments arranged by size: 1877 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3507 493 Size: 3014 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5716 SphI G_CATG'C Cuts at: 1132 3074 1132 Size: 1942 3774 SspI AAT'ATT Cuts at: 5 52 3640 5 Size: 47 3588 2081 Fragments arranged by size: 3588 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5716 StyI C'CwwG_G Cuts at: 513 1065 2004 2166 3155 513 Size: 552 939 162 989 3074 Fragments arranged by size: 3074 989 939 552 162 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2167 2198 3743 3760 3913 4098 4543 Size: 1255 587 31 1545 17 153 185 445 Cuts at: 4543 325 Size: 1498 Fragments arranged by size: 1545 1498 1255 587 445 185 153 31 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3962 5674 96 Size: 274 80 33 51 1443 1985 1712 138 Fragments arranged by size: 1985 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2194 2226 2234 2266 5676 5708 2194 Size: 32 8 32 3410 32 2202 Fragments arranged by size: 3410 2202 32 32 32 8 Tth111I GACn'n_nGTC Cuts at: 1316 2996 1316 Size: 1680 4036 XbaI T'CTAG_A Cuts at: 3052 3052 Size: 5716 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2143 2306 2992 2143 Size: 163 686 4867 Fragments arranged by size: 4867 686 163 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5716 XmaI C'CCGG_G Cuts at: 1870 3041 1870 Size: 1171 4545 XmnI GAAnn'nnTTC Cuts at: 3845 3845 Size: 5716 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3524 276 Size: 53 83 186 936 1990 2468 Fragments arranged by size: 2468 1990 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI SacI SacII SanDI SfiI SrfI SwaI