################################## # NO CUT ENZYMES ( 197 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiHKAI BsiWI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DpnII DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HapII HincII HindII HindIII HinfI HpaI HpaII KasI KpnI Ksp632I MaeI MaeII MamI MboI MboII MfeI MflI MluI MroI MseI MspI MunI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp509I TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AcyI 150 GACGCC G[GA]^CG[CT]C AfaI 157 GTAC GT^AC Asp700I 171 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 14 TGGCCA TGG^CCA BcgI 120 CGAGACGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpmI 58 CTGGAG CTGGAG(16/14) BsaHI 150 GACGCC G[GA]^CG[CT]C BsiEI 72 CGACCG CG[GA][CT]^CG BsmAI 121 GAGAC GTCTC(1/5) BsmBI 121 GAGACG CGTCTC(1/5) BsrI 154 CCAGT ACTGG(1/-1) BssKI 108 CCTGG ^CC[ACGT]GG BstNI 108 CCTGG CC^[AT]GG BstXI 5 CCAACAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 189 GCGC GCG^C Cfr13I 30 GGCCC G^G[ACGT]CC DsaI 164 CCGCGG C^C[GA][CT]GG EaeI 14 TGGCCA [CT]^GGCC[GA] EcoRII 108 CCTGG ^CC[AT]GG HaeII 188 AGCGCC [GA]GCGC^[CT] HhaI 189 GCGC GCG^C Hin1I 150 GACGCC G[GA]^CG[CT]C HinP1I 189 GCGC G^CGC KspI 164 CCGCGG CCGC^GG MaeIII 148 GTGAC ^GT[ACGT]AC MluNI 14 TGGCCA TGG^CCA MscI 14 TGGCCA TGG^CCA MslI 78 CATGAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvaI 108 CCTGG CC^[AT]GG NspI 126 GCATGC [GA]CATG^[CT] PvuII 49 CAGCTG CAG^CTG RsaI 157 GTAC GT^AC SacII 164 CCGCGG CCGC^GG Sau96I 30 GGCCC G^G[ACGT]CC ScrFI 108 CCTGG CC^[ACGT]GG SphI 126 GCATGC GCATG^C TaqI 71 TCGA T^CGA Tsp45I 148 GTGAC ^GT[GC]AC TthHB8I 71 TCGA T^CGA XmnI 171 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AccII 165 CGCG CG^CG 181 CGCG AluI 50 AGCT AG^CT 56 AGCT BbvI 94 GCAGC GCAGC(8/12) 97 GCAGC BsaJI 26 CCGAGG C^C[ACGT][ACGT]GG 164 CCGCGG BsiYI 33 CCGCAAGACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 75 CCGCATGAAGG BslI 33 CCGCAAGACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 75 CCGCATGAAGG BstUI 165 CGCG CG^CG 181 CGCG HaeIII 15 GGCC GG^CC 30 GGCC HgaI 123 GACGC GACGC(5/10) 150 GACGC HphI 63 GGTGA GGTGA(8/7) 147 GGTGA MvnI 165 CGCG CG^CG 181 CGCG TseI 94 GCAGC G^C[AT]GC 97 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 9 enzymes) # ######################################### AciI Cac8I FauI Fnu4HI ItaI MnlI MspA1I MwoI NlaIII