################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BciVI BclI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BsrI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DpnII DraI DrdI Eam1105I EarI Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI HphI KpnI Ksp632I MaeIII MamI MboI MboII MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NaeI NcoI NdeI NdeII NgoMIV NheI NlaIII NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfcI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TaqI Tru9I Tsp45I Tsp509I TspRI Tth111I TthHB8I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AccI 391 GTCTAC GT^[AC][GT]AC AfaI 117 GTAC GT^AC AluI 113 AGCT AG^CT Aor51HI 83 AGCGCT AGC^GCT ApaI 132 GGGCCC GGGCC^C AvaI 591 CCCGGG C^[CT]CG[GA]G BanII 132 GGGCCC G[GA]GC[CT]^C BbeI 300 GGCGCC GGCGC^C BfaI 428 CTAG C^TAG BsaI 184 GAGACC GGTCTC(1/5) BsoBI 591 CCCGGG C^[CT]CG[GA]G BspMI 236 ACCTGC ACCTGC(4/8) BsrBI 384 GAGCGG CCGCTC(-3/-3) BssSI 247 CTCGTG CACGAG(-5/-1) BstF5I 555 CATCC GGATG(2/0) DdeI 581 CTGAG C^T[ACGT]AG Eco47III 83 AGCGCT AGC^GCT FokI 555 CATCC GGATG(9/13) KasI 300 GGCGCC G^GCGCC MaeI 428 CTAG C^TAG NarI 300 GGCGCC GG^CGCC PflMI 363 CCACGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 190 GACTC GAGTC(4/5) RsaI 117 GTAC GT^AC SfoI 300 GGCGCC GGC^GCC SmaI 591 CCCGGG CCC^GGG StuI 275 AGGCCT AGG^CCT Van91I 363 CCACGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 591 CCCGGG C^CCGGG XmaI 591 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AcyI 3 GGCGTC G[GA]^CG[CT]C 300 GGCGCC BanI 51 GGTGCC G^G[CT][GA]CC 300 GGCGCC BcgI 81 CGAGCGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 230 CGAGGGACCTGC BmyI 132 GGGCCC G[AGT]GC[ACT]^C 282 GGGCAC BsaHI 3 GGCGTC G[GA]^CG[CT]C 300 GGCGCC BsgI 253 GTGCAG GTGCAG(16/14) 401 GTGCAG BsmBI 43 GAGACG CGTCTC(1/5) 70 GAGACG Bsp1286I 132 GGGCCC G[AGT]GC[ACT]^C 282 GGGCAC BssHII 313 GCGCGC G^CGCGC 487 GCGCGC DraIII 364 CACGACGTG CAC[ACGT][ACGT][ACGT]^GTG 472 CACAGCGTG HaeII 83 AGCGCT [GA]GCGC^[CT] 300 GGCGCC Hin1I 3 GGCGTC G[GA]^CG[CT]C 300 GGCGCC MaeII 368 ACGT A^CGT 413 ACGT NotI 143 GCGGCCGC GC^GGCCGC 373 GCGGCCGC TfiI 76 GATTC G^A[AT]TC 262 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AccII AciI AvaII BbvI BcnI BglI BsaJI BseRI BsiEI BsiYI BslI BsmAI BsmFI BssKI BstNI BstUI Cac8I CfoI Cfr13I DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII ItaI KspI MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIV PpuMI SacII Sau96I ScrFI TseI