(Linear) (Six-base) MAPSORT of: KIEE6592.seq Check: 2533 from: 1 to: 606 KIEE6592.orf With 182 enzymes: SgfI * March 16, 2015 11:34 .. AccI GT'mk_AC Cuts at: 0 392 606 Size: 392 214 AfeI AGC'GCT Cuts at: 0 85 606 Size: 85 521 ApaI G_GGCC'C Cuts at: 0 136 606 Size: 136 470 AvaI C'yCGr_G Cuts at: 0 591 606 Size: 591 15 BanI G'GyrC_C Cuts at: 0 51 300 606 Size: 51 249 306 Fragments arranged by size: 306 249 51 BanII G_rGCy'C Cuts at: 0 136 606 Size: 136 470 BbeI G_GCGC'C Cuts at: 0 304 606 Size: 304 302 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 70 104 219 253 606 Size: 70 34 115 34 353 Fragments arranged by size: 353 115 70 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 404 424 451 606 Size: 404 20 27 155 Fragments arranged by size: 404 155 27 20 Bme1580I G_kGCm'C Cuts at: 0 136 286 606 Size: 136 150 320 Fragments arranged by size: 320 150 136 BmgBI CAC'GTC Cuts at: 0 369 414 606 Size: 369 45 192 Fragments arranged by size: 369 192 45 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 207 606 Size: 207 399 BsaI GGTCTCn'nnnn_ Cuts at: 0 178 606 Size: 178 428 BsaHI Gr'CG_yC Cuts at: 0 4 301 606 Size: 4 297 305 Fragments arranged by size: 305 297 4 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 100 130 606 Size: 100 30 476 Fragments arranged by size: 476 100 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 118 121 124 286 304 606 Size: 118 3 3 162 18 302 Fragments arranged by size: 302 162 118 18 3 3 BseYI C'CCAG_C Cuts at: 0 169 606 Size: 169 437 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 274 422 606 Size: 274 148 184 Fragments arranged by size: 274 184 148 BsiEI CG_ry'CG Cuts at: 0 68 147 224 377 606 Size: 68 79 77 153 229 Fragments arranged by size: 229 153 79 77 68 BsmBI CGTCTCn'nnnn_ Cuts at: 0 37 64 606 Size: 37 27 542 Fragments arranged by size: 542 37 27 Bsp1286I G_dGCh'C Cuts at: 0 136 286 606 Size: 136 150 320 Fragments arranged by size: 320 150 136 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 245 606 Size: 245 361 BsrBI CCG'CTC Cuts at: 0 386 606 Size: 386 220 BssHII G'CGCG_C Cuts at: 0 313 487 606 Size: 313 174 119 Fragments arranged by size: 313 174 119 BssSI C'ACGA_G Cuts at: 0 247 606 Size: 247 359 BtgI C'CryG_G Cuts at: 0 152 179 558 598 606 Size: 152 27 379 40 8 Fragments arranged by size: 379 152 40 27 8 DraIII CAC_nnn'GTG Cuts at: 0 369 477 606 Size: 369 108 129 Fragments arranged by size: 369 129 108 EaeI y'GGCC_r Cuts at: 0 144 221 374 416 509 606 Size: 144 77 153 42 93 97 Fragments arranged by size: 153 144 97 93 77 42 EagI C'GGCC_G Cuts at: 0 144 221 374 606 Size: 144 77 153 232 Fragments arranged by size: 232 153 144 77 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 36 283 355 606 Size: 36 247 72 251 Fragments arranged by size: 251 247 72 36 EcoO109I rG'GnC_Cy Cuts at: 0 26 124 234 294 495 606 Size: 26 98 110 60 201 111 Fragments arranged by size: 201 111 110 98 60 26 HaeII r_GCGC'y Cuts at: 0 87 304 606 Size: 87 217 302 Fragments arranged by size: 302 217 87 KasI G'GCGC_C Cuts at: 0 300 606 Size: 300 306 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 80 606 Size: 80 526 MspA1I CmG'CkG Cuts at: 0 181 258 560 600 606 Size: 181 77 302 40 6 Fragments arranged by size: 302 181 77 40 6 NarI GG'CG_CC Cuts at: 0 301 606 Size: 301 305 NotI GC'GGCC_GC Cuts at: 0 144 374 606 Size: 144 230 232 Fragments arranged by size: 232 230 144 PasI CC'CwG_GG Cuts at: 0 9 606 Size: 9 597 PflMI CCAn_nnn'nTGG Cuts at: 0 369 606 Size: 369 237 PpuMI rG'GwC_Cy Cuts at: 0 124 234 495 606 Size: 124 110 261 111 Fragments arranged by size: 261 124 111 110 PspOMI G'GGCC_C Cuts at: 0 132 606 Size: 132 474 SacII CC_GC'GG Cuts at: 0 182 561 601 606 Size: 182 379 40 5 Fragments arranged by size: 379 182 40 5 SfoI GGC'GCC Cuts at: 0 302 606 Size: 302 304 SmaI CCC'GGG Cuts at: 0 593 606 Size: 593 13 StuI AGG'CCT Cuts at: 0 277 606 Size: 277 329 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 82 202 589 606 Size: 82 120 387 17 Fragments arranged by size: 387 120 82 17 XmaI C'CCGG_G Cuts at: 0 591 606 Size: 591 15 Enzymes that do cut: AccI AfeI ApaI AvaI BanI BanII BbeI BcgI BglI Bme1580I BmgBI BplI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsmBI Bsp1286I BspMI BsrBI BssHII BssSI BtgI DraIII EaeI EagI EciI EcoO109I HaeII KasI MmeI MspA1I NarI NotI PasI PflMI PpuMI PspOMI SacII SfoI SmaI StuI TaqII XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BciVI BclI BfrBI BglII BlpI BmrI BmtI BpmI Bpu10I BpuEI BsaAI BsaBI BsaWI BsiHKAI BsiWI BsmI BspEI BspHI BsrDI BsrFI BsrGI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EarI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MscI MslI NaeI NcoI NdeI NgoMIV NheI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StyI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI