################################## # NO CUT ENZYMES ( 180 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DpnII DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MboI MboII MfeI MflI MluI MroI MseI MunI MvnI NaeI NarI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AflIII 308 ACGTGT A^C[GA][CT]GT ApaI 48 GGGCCC GGGCC^C AspHI 31 GTGCTC G[AT]GC[AT]^C AvaI 44 CCCGGG C^[CT]CG[GA]G BglI 50 GCCCTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 8 CTCCAG CTGGAG(16/14) BsaAI 307 TACGTG [CT]AC^GT[GA] BsiHKAI 31 GTGCTC G[AT]GC[AT]^C BsmFI 131 GTCCC GGGAC(10/14) BsoBI 44 CCCGGG C^[CT]CG[GA]G BsrI 10 CCAGT ACTGG(1/-1) BstF5I 291 GGATG GGATG(2/0) BstXI 281 CCACCGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 349 GCGC GCG^C EcoT14I 215 CCATGG C^C[AT][AT]GG FauI 168 GCGGG CCCGC(4/6) FokI 291 GGATG GGATG(9/13) HaeII 348 GGCGCT [GA]GCGC^[CT] HgaI 86 GACGC GACGC(5/10) HhaI 349 GCGC GCG^C HinP1I 349 GCGC G^CGC HphI 231 GGTGA GGTGA(8/7) NcoI 215 CCATGG C^CATGG NlaIII 216 CATG CATG^ PleI 324 GACTC GAGTC(4/5) PstI 319 CTGCAG CTGCA^G PvuII 316 CAGCTG CAG^CTG SfcI 319 CTGCAG C^T[GA][CT]AG SmaI 44 CCCGGG CCC^GGG StyI 215 CCATGG C^C[AT][AT]GG TspRI 11 CAGTG CA[GC]TG[ACGT][ACGT]^ XmaCI 44 CCCGGG C^CCGGG XmaI 44 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### BalI 212 TGGCCA TGG^CCA 296 TGGCCA BanII 48 GGGCCC G[GA]GC[CT]^C 92 GAGCCC BcnI 44 CCCGG CC^[GC]GG 45 CCGGG BssSI 173 CACGAG CACGAG(-5/-1) 247 CACGAG Cfr13I 48 GGGCC G^G[ACGT]CC 49 GGCCC DraII 47 GGGGCCC [GA]G^G[ACGT]CC[CT] 48 GGGCCCT DsaI 66 CCGTGG C^C[GA][CT]GG 215 CCATGG EcoO109I 47 GGGGCCC [GA]G^G[ACGT]CC[CT] 48 GGGCCCT HapII 36 CCGG C^CGG 45 CCGG HpaII 36 CCGG C^CGG 45 CCGG MaeII 241 ACGT A^CGT 308 ACGT MluNI 212 TGGCCA TGG^CCA 296 TGGCCA MscI 212 TGGCCA TGG^CCA 296 TGGCCA MspA1I 166 CAGCGG C[AC]G^C[GT]G 316 CAGCTG MspI 36 CCGG C^CGG 45 CCGG NciI 44 CCCGG CC^[GC]GG 45 CCGGG Sau96I 48 GGGCC G^G[ACGT]CC 49 GGCCC SmlI 99 CTTGAG C^T[CT][GA]AG 140 CTCAAG TfiI 145 GAATC G^A[AT]TC 154 GAATC Tsp45I 312 GTCAC ^GT[GC]AC 365 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AciI AluI BbvI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI Cac8I EaeI EcoRII Fnu4HI HaeIII HinfI ItaI MaeIII MnlI MslI MvaI MwoI NlaIV ScrFI TseI