(Circular) (Six-base) MAPSORT of: pFN21AE2533.seq Check: 3953 from: 1 to: 6283 pFN21AE2533 6283 bp With 182 enzymes: SgfI * August 16, 2021 15:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2809 2809 Size: 6283 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4093 278 Size: 53 83 186 936 2557 2468 Fragments arranged by size: 2557 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2424 3626 1937 Size: 487 1202 4594 Fragments arranged by size: 4594 1202 487 Acc65I G'GTAC_C Cuts at: 3604 3604 Size: 6283 AclI AA'CG_TT Cuts at: 1563 4410 4783 1563 Size: 2847 373 3063 Fragments arranged by size: 3063 2847 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2721 3510 4346 5730 5998 1632 Size: 1089 789 836 1384 268 1917 Fragments arranged by size: 1917 1384 1089 836 789 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6086 AflIII A'CryG_T Cuts at: 1203 5182 6159 1203 Size: 3979 977 1327 Fragments arranged by size: 3979 1327 977 AgeI A'CCGG_T Cuts at: 5114 5114 Size: 6283 AhdI GACnn_n'nnGTC Cuts at: 5012 5012 Size: 6283 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6283 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6283 AlwNI CAG_nnn'CTG Cuts at: 2921 4026 5598 2921 Size: 1105 1572 3606 Fragments arranged by size: 3606 1572 1105 ApaI G_GGCC'C Cuts at: 2283 2283 Size: 6283 ApaLI G'TGCA_C Cuts at: 4340 5496 4340 Size: 1156 5127 ApoI r'AATT_y Cuts at: 1493 3592 3818 3970 6079 1493 Size: 2099 226 152 2109 1697 Fragments arranged by size: 2109 2099 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4837 160 Size: 872 3805 1606 Fragments arranged by size: 3805 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6283 AvaI C'yCGr_G Cuts at: 1870 1943 3608 1870 Size: 73 1665 4545 Fragments arranged by size: 4545 1665 73 BamHI G'GATC_C Cuts at: 2888 3613 3980 2888 Size: 725 367 5191 Fragments arranged by size: 5191 725 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2010 3604 5059 618 Size: 325 217 627 223 1594 1455 1842 Fragments arranged by size: 1842 1594 1455 627 325 223 217 BanII G_rGCy'C Cuts at: 1391 2283 2856 3602 1391 Size: 892 573 746 4072 Fragments arranged by size: 4072 892 746 573 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3190 3377 928 Size: 971 1291 187 3834 Fragments arranged by size: 3834 1291 971 187 BbvCI CC'TCA_GC Cuts at: 2768 3146 2768 Size: 378 5905 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4474 4508 1903 Size: 34 2537 34 3678 Fragments arranged by size: 3678 2537 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4175 5385 4175 Size: 1210 5073 BclI T'GATC_A Cuts at: 1548 1548 Size: 6283 BfrBI ATG'CAT Cuts at: 2318 2318 Size: 6283 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4894 136 Size: 107 122 71 631 605 3222 1525 Fragments arranged by size: 3222 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2386 6278 2386 Size: 3892 2391 BlpI GC'TnA_GC Cuts at: 3700 3700 Size: 6283 Bme1580I G_kGCm'C Cuts at: 1790 2013 2283 4344 5500 1790 Size: 223 270 2061 1156 2573 Fragments arranged by size: 2573 2061 1156 270 223 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6283 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2657 2681 4014 Size: 416 53 83 399 24 1240 24 1333 Cuts at: 4014 4972 442 Size: 958 1753 Fragments arranged by size: 1753 1333 1240 958 416 399 83 53 24 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6283 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2437 4943 1239 Size: 1198 2506 2579 Fragments arranged by size: 2579 2506 1198 Bpu10I CC'TnA_GC Cuts at: 2768 3146 2768 Size: 378 5905 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4408 5273 5571 4408 Size: 865 298 5120 Fragments arranged by size: 5120 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4946 882 Size: 617 3447 2219 Fragments arranged by size: 3447 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4074 493 Size: 711 2870 2702 Fragments arranged by size: 2870 2702 711 BsaBI GATnn'nnATC Cuts at: 6277 6277 Size: 6283 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4090 4472 5107 275 Size: 53 83 186 936 2557 382 635 1451 Fragments arranged by size: 2557 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4716 5114 5388 5535 5946 4716 Size: 398 274 147 411 5053 Fragments arranged by size: 5053 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6058 Fragments arranged by size: 6058 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6283 BseYI C'CCAG_C Cuts at: 2106 2181 3176 3504 5486 5982 2106 Size: 75 995 328 1982 496 2407 Fragments arranged by size: 2407 1982 995 496 328 75 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2787 1163 Size: 573 1051 4659 Fragments arranged by size: 4659 1051 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2296 3733 4494 5522 1540 Size: 275 186 295 1437 761 1028 2301 Fragments arranged by size: 2301 1437 1028 761 295 275 186 BsiHKAI G_wGCw'C Cuts at: 2856 3411 4344 4429 5500 2856 Size: 555 933 85 1071 3639 Fragments arranged by size: 3639 1071 933 555 85 BsmI GAATG_Cn' Cuts at: 3794 3887 3794 Size: 93 6190 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2283 2856 3411 3602 4344 4429 Size: 399 223 270 573 555 191 742 85 Cuts at: 4429 5500 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 573 555 399 270 223 191 85 BspEI T'CCGG_A Cuts at: 5946 5946 Size: 6283 BspHI T'CATG_A Cuts at: 4172 5902 4172 Size: 1730 4553 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2809 2906 3053 3571 3638 844 Size: 1965 97 147 518 67 3489 Fragments arranged by size: 3489 1965 518 147 97 67 BsrBI CCG'CTC Cuts at: 1394 1823 4170 1394 Size: 429 2347 3507 Fragments arranged by size: 3507 2347 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3291 4778 4952 65 Size: 1183 2043 1487 174 1396 Fragments arranged by size: 2043 1487 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3374 4927 5114 1700 Size: 110 1564 1553 187 2869 Fragments arranged by size: 2869 1564 1553 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6283 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6283 BssSI C'ACGA_G Cuts at: 4343 5355 4343 Size: 1012 5271 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6283 BstBI TT'CG_AA Cuts at: 5087 5087 Size: 6283 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6283 BstXI CCAn_nnnn'nTGG Cuts at: 2473 2473 Size: 6283 BstYI r'GATC_y Cuts at: 1972 2386 2888 3613 3980 4365 4382 5834 5920 Size: 414 502 725 367 385 17 1452 86 Cuts at: 5920 5932 6278 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 725 502 414 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2425 2425 Size: 6283 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4792 Fragments arranged by size: 4792 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5544 Fragments arranged by size: 5544 683 56 BtsI GCAGTG_nn' Cuts at: 2100 2686 3799 4593 4613 2100 Size: 586 1113 794 20 3770 Fragments arranged by size: 3770 1113 794 586 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6283 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6248 DraI TTT'AAA Cuts at: 3587 3587 Size: 6283 DraIII CAC_nnn'GTG Cuts at: 2035 2035 Size: 6283 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5290 809 Size: 507 3974 1802 Fragments arranged by size: 3974 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3730 4619 6248 Size: 54 1425 50 275 18 1900 889 1629 Cuts at: 6248 8 Size: 43 Fragments arranged by size: 1900 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3730 1537 Size: 275 1918 4090 Fragments arranged by size: 4090 1918 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4213 1353 Size: 2860 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4870 5244 5390 387 Size: 1064 138 3281 374 146 1280 Fragments arranged by size: 3281 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2854 2854 Size: 6283 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2437 2721 3510 4346 4943 5730 5998 Size: 393 805 284 789 836 597 787 268 Cuts at: 5998 1239 Size: 1524 Fragments arranged by size: 1524 836 805 789 787 597 393 284 268 EcoNI CCTnn'n_nnAGG Cuts at: 3112 3112 Size: 6283 EcoO109I rG'GnC_Cy Cuts at: 2279 2598 2636 2279 Size: 319 38 5926 Fragments arranged by size: 5926 319 38 EcoRI G'AATT_C Cuts at: 3592 3970 3592 Size: 378 5905 FspI TGC'GCA Cuts at: 4789 4789 Size: 6283 HaeII r_GCGC'y Cuts at: 1678 5430 1678 Size: 3752 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2533 2565 2590 2622 3014 Size: 32 135 32 961 32 25 32 392 Cuts at: 3014 3046 4924 4956 4998 5030 1373 Size: 32 1878 32 42 32 2626 Fragments arranged by size: 2626 1878 961 392 135 42 32 32 32 32 32 32 32 25 HincII GTy'rAC Cuts at: 669 1657 1938 3627 3873 669 Size: 988 281 1689 246 3079 Fragments arranged by size: 3079 1689 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6283 HpaI GTT'AAC Cuts at: 3873 3873 Size: 6283 KpnI G_GTAC'C Cuts at: 3608 3608 Size: 6283 MfeI C'AATT_G Cuts at: 3882 3882 Size: 6283 MluI A'CGCG_T Cuts at: 6159 6159 Size: 6283 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2490 3450 5397 5581 1305 Size: 1185 960 1947 184 2007 Fragments arranged by size: 2007 1947 1185 960 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4461 Fragments arranged by size: 4461 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2873 3447 4241 4600 4759 518 Size: 648 1707 574 794 359 159 2042 Fragments arranged by size: 2042 1707 794 648 574 359 159 MspA1I CmG'CkG Cuts at: 1606 2202 2292 2581 3699 4376 5524 5769 1606 Size: 596 90 289 1118 677 1148 245 2120 Fragments arranged by size: 2120 1148 1118 677 596 289 245 90 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4792 Fragments arranged by size: 4792 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6283 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6283 NotI GC'GGCC_GC Cuts at: 3730 3730 Size: 6283 NsiI A_TGCA'T Cuts at: 2320 2320 Size: 6283 NspI r_CATG'y Cuts at: 1132 1229 3354 3641 5186 1132 Size: 97 2125 287 1545 2229 Fragments arranged by size: 2229 2125 1545 287 97 PasI CC'CwG_GG Cuts at: 1348 2276 3130 1348 Size: 928 854 4501 Fragments arranged by size: 4501 928 854 PciI A'CATG_T Cuts at: 5182 5182 Size: 6283 PflMI CCAn_nnn'nTGG Cuts at: 1108 3078 1108 Size: 1970 4313 PmeI GTTT'AAAC Cuts at: 3587 3587 Size: 6283 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4352 4384 5865 5897 4352 Size: 32 1481 32 4738 Fragments arranged by size: 4738 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2598 2636 2598 Size: 38 6245 PshAI GACnn'nnGTC Cuts at: 1613 2939 3011 1613 Size: 1326 72 4885 Fragments arranged by size: 4885 1326 72 PsiI TTA'TAA Cuts at: 3853 3853 Size: 6283 PspOMI G'GGCC_C Cuts at: 2279 2279 Size: 6283 PstI C_TGCA'G Cuts at: 830 2580 3635 830 Size: 1750 1055 3478 Fragments arranged by size: 3478 1750 1055 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6283 PvuII CAG'CTG Cuts at: 2202 2581 2202 Size: 379 5904 SacI G_AGCT'C Cuts at: 2856 2856 Size: 6283 SalI G'TCGA_C Cuts at: 1936 3625 1936 Size: 1689 4594 SbfI CC_TGCA'GG Cuts at: 3635 3635 Size: 6283 ScaI AGT'ACT Cuts at: 2810 4531 6243 2810 Size: 1721 1712 2850 Fragments arranged by size: 2850 1721 1712 SfcI C'TryA_G Cuts at: 826 1045 2197 2576 3631 4766 5447 5638 826 Size: 219 1152 379 1055 1135 681 191 1471 Fragments arranged by size: 1471 1152 1135 1055 681 379 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6283 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6283 SmaI CCC'GGG Cuts at: 1872 3610 1872 Size: 1738 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4387 5288 5550 820 Size: 197 926 2444 901 262 1553 Fragments arranged by size: 2444 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4074 493 Size: 3581 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6283 SphI G_CATG'C Cuts at: 1132 3641 1132 Size: 2509 3774 SspI AAT'ATT Cuts at: 5 52 4207 5 Size: 47 4155 2081 Fragments arranged by size: 4155 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6283 StyI C'CwwG_G Cuts at: 513 1065 2004 3722 513 Size: 552 939 1718 3074 Fragments arranged by size: 3074 1718 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2357 4310 4327 4480 4665 5110 325 Size: 1255 777 1953 17 153 185 445 1498 Fragments arranged by size: 1953 1498 1255 777 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2808 4529 6241 Size: 274 80 33 51 1443 831 1721 1712 Cuts at: 6241 96 Size: 138 Fragments arranged by size: 1721 1712 1443 831 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6243 6275 6243 Size: 32 6251 Tth111I GACn'n_nGTC Cuts at: 1316 2687 1316 Size: 1371 4912 XbaI T'CTAG_A Cuts at: 3619 3619 Size: 6283 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2189 2189 Size: 6283 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6283 XmaI C'CCGG_G Cuts at: 1870 3608 1870 Size: 1738 4545 XmnI GAAnn'nnTTC Cuts at: 4412 4412 Size: 6283 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4091 276 Size: 53 83 186 936 2557 2468 Fragments arranged by size: 2557 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI