(Linear) (Six-base) MAPSORT of: KIEE4201.seq Check: 237 from: 1 to: 2121 KIEE4201.orf With 182 enzymes: SgfI * March 22, 2012 17:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1759 2121 Size: 1759 362 AccI GT'mk_AC Cuts at: 0 1145 2121 Size: 1145 976 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1820 2121 Size: 1820 301 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 505 2121 Size: 505 1616 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 862 894 2121 Size: 862 32 1227 Fragments arranged by size: 1227 862 32 AlwNI CAG_nnn'CTG Cuts at: 0 469 2007 2121 Size: 469 1538 114 Fragments arranged by size: 1538 469 114 ApaLI G'TGCA_C Cuts at: 0 1282 2121 Size: 1282 839 AvaI C'yCGr_G Cuts at: 0 49 734 1251 2121 Size: 49 685 517 870 Fragments arranged by size: 870 685 517 49 BamHI G'GATC_C Cuts at: 0 116 252 2121 Size: 116 136 1869 Fragments arranged by size: 1869 136 116 BanI G'GyrC_C Cuts at: 0 861 1198 1539 1967 2121 Size: 861 337 341 428 154 Fragments arranged by size: 861 428 341 337 154 BanII G_rGCy'C Cuts at: 0 308 813 1103 1252 1657 1942 2121 Size: 308 505 290 149 405 285 179 Fragments arranged by size: 505 405 308 290 285 179 149 BbeI G_GCGC'C Cuts at: 0 1971 2121 Size: 1971 150 BbsI GAAGACnn'nnnn_ Cuts at: 0 133 204 2121 Size: 133 71 1917 Fragments arranged by size: 1917 133 71 BbvCI CC'TCA_GC Cuts at: 0 443 1259 1958 2121 Size: 443 816 699 163 Fragments arranged by size: 816 699 443 163 BglI GCCn_nnn'nGGC Cuts at: 0 1828 2121 Size: 1828 293 Bme1580I G_kGCm'C Cuts at: 0 1286 1544 2121 Size: 1286 258 577 Fragments arranged by size: 1286 577 258 BmrI ACTGGGnnnn_n' Cuts at: 0 29 288 400 627 1538 1589 2121 Size: 29 259 112 227 911 51 532 Fragments arranged by size: 911 532 259 227 112 51 29 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 120 1465 1663 1890 2046 2121 Size: 120 1345 198 227 156 75 Fragments arranged by size: 1345 227 198 156 120 75 Bpu10I CC'TnA_GC Cuts at: 0 443 1206 1259 1958 2121 Size: 443 763 53 699 163 Fragments arranged by size: 763 699 443 163 53 BsaI GGTCTCn'nnnn_ Cuts at: 0 169 2074 2121 Size: 169 1905 47 Fragments arranged by size: 1905 169 47 BsaAI yAC'GTr Cuts at: 0 1044 1356 2121 Size: 1044 312 765 Fragments arranged by size: 1044 765 312 BsaBI GATnn'nnATC Cuts at: 0 1186 2121 Size: 1186 935 BsaHI Gr'CG_yC Cuts at: 0 1968 2121 Size: 1968 153 BsaWI w'CCGG_w Cuts at: 0 178 2121 Size: 178 1943 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 862 892 1015 1045 1888 1918 2121 Size: 862 30 123 30 843 30 203 Fragments arranged by size: 862 843 203 123 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 271 302 355 586 1000 2121 Size: 271 31 53 231 414 1121 Fragments arranged by size: 1121 414 271 231 53 31 BseYI C'CCAG_C Cuts at: 0 519 1113 1400 1861 2121 Size: 519 594 287 461 260 Fragments arranged by size: 594 519 461 287 260 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 991 1072 1249 2121 Size: 991 81 177 872 Fragments arranged by size: 991 872 177 81 BsiHKAI G_wGCw'C Cuts at: 0 1103 1286 1597 2121 Size: 1103 183 311 524 Fragments arranged by size: 1103 524 311 183 BsmI GAATG_Cn' Cuts at: 0 533 942 2121 Size: 533 409 1179 Fragments arranged by size: 1179 533 409 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1573 2121 Size: 1573 548 Bsp1286I G_dGCh'C Cuts at: 0 308 813 1103 1252 1286 1544 1597 1657 Size: 308 505 290 149 34 258 53 60 Cuts at: 1657 1942 2121 Size: 285 179 Fragments arranged by size: 505 308 290 285 258 179 149 60 53 34 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 395 948 1697 1759 2121 Size: 395 553 749 62 362 Fragments arranged by size: 749 553 395 362 62 BsrBI CCG'CTC Cuts at: 0 149 1506 2121 Size: 149 1357 615 Fragments arranged by size: 1357 615 149 BsrDI GCAATG_nn' Cuts at: 0 513 899 1488 2121 Size: 513 386 589 633 Fragments arranged by size: 633 589 513 386 BsrFI r'CCGG_y Cuts at: 0 422 787 1827 2121 Size: 422 365 1040 294 Fragments arranged by size: 1040 422 365 294 BstEII G'GTnAC_C Cuts at: 0 382 1128 2121 Size: 382 746 993 Fragments arranged by size: 993 746 382 BstXI CCAn_nnnn'nTGG Cuts at: 0 249 301 1743 2054 2121 Size: 249 52 1442 311 67 Fragments arranged by size: 1442 311 249 67 52 BstYI r'GATC_y Cuts at: 0 116 252 797 1433 2121 Size: 116 136 545 636 688 Fragments arranged by size: 688 636 545 136 116 Bsu36I CC'TnA_GG Cuts at: 0 558 569 2121 Size: 558 11 1552 Fragments arranged by size: 1552 558 11 BtgI C'CryG_G Cuts at: 0 267 1383 1607 1742 1932 1971 2053 2121 Size: 267 1116 224 135 190 39 82 68 Fragments arranged by size: 1116 267 224 190 135 82 68 39 BtsI GCAGTG_nn' Cuts at: 0 1479 2121 Size: 1479 642 ClaI AT'CG_AT Cuts at: 0 351 2121 Size: 351 1770 DrdI GACnn_nn'nnGTC Cuts at: 0 208 1090 2121 Size: 208 882 1031 Fragments arranged by size: 1031 882 208 EaeI y'GGCC_r Cuts at: 0 460 1698 1718 1755 1926 2035 2121 Size: 460 1238 20 37 171 109 86 Fragments arranged by size: 1238 460 171 109 86 37 20 EarI CTCTTCn'nnn_ Cuts at: 0 379 1270 1564 2121 Size: 379 891 294 557 Fragments arranged by size: 891 557 379 294 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 432 835 2121 Size: 432 403 1286 Fragments arranged by size: 1286 432 403 EcoICRI GAG'CTC Cuts at: 0 1101 2121 Size: 1101 1020 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 120 1465 1663 1820 1890 2046 2121 Size: 120 1345 198 157 70 156 75 Fragments arranged by size: 1345 198 157 156 120 75 70 EcoNI CCTnn'n_nnAGG Cuts at: 0 1756 2121 Size: 1756 365 EcoO109I rG'GnC_Cy Cuts at: 0 30 43 201 439 1110 1255 1728 1761 Size: 30 13 158 238 671 145 473 33 Cuts at: 1761 2121 Size: 360 Fragments arranged by size: 671 473 360 238 158 145 33 30 13 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1080 2121 Size: 1080 1041 FspI TGC'GCA Cuts at: 0 238 1069 2121 Size: 238 831 1052 Fragments arranged by size: 1052 831 238 HaeII r_GCGC'y Cuts at: 0 727 1686 1971 2121 Size: 727 959 285 150 Fragments arranged by size: 959 727 285 150 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 710 742 1425 1457 2121 Size: 710 32 683 32 664 Fragments arranged by size: 710 683 664 32 32 KasI G'GCGC_C Cuts at: 0 1967 2121 Size: 1967 154 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 230 326 498 1295 2055 2121 Size: 230 96 172 797 760 66 Fragments arranged by size: 797 760 230 172 96 66 MscI TGG'CCA Cuts at: 0 1700 1720 1928 2121 Size: 1700 20 208 193 Fragments arranged by size: 1700 208 193 20 MslI CAynn'nnrTG Cuts at: 0 68 928 1741 2121 Size: 68 860 813 380 Fragments arranged by size: 860 813 380 68 MspA1I CmG'CkG Cuts at: 0 10 678 1117 1385 1587 1803 2066 2121 Size: 10 668 439 268 202 216 263 55 Fragments arranged by size: 668 439 268 263 216 202 55 10 NaeI GCC'GGC Cuts at: 0 1829 2121 Size: 1829 292 NarI GG'CG_CC Cuts at: 0 1968 2121 Size: 1968 153 NcoI C'CATG_G Cuts at: 0 1742 2121 Size: 1742 379 NgoMIV G'CCGG_C Cuts at: 0 1827 2121 Size: 1827 294 PasI CC'CwG_GG Cuts at: 0 257 2121 Size: 257 1864 PflMI CCAn_nnn'nTGG Cuts at: 0 953 1536 2007 2121 Size: 953 583 471 114 Fragments arranged by size: 953 583 471 114 PfoI T'CCnGG_A Cuts at: 0 962 2121 Size: 962 1159 PmlI CAC'GTG Cuts at: 0 1356 2121 Size: 1356 765 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 862 894 2121 Size: 862 32 1227 Fragments arranged by size: 1227 862 32 PpuMI rG'GwC_Cy Cuts at: 0 30 201 1110 1728 2121 Size: 30 171 909 618 393 Fragments arranged by size: 909 618 393 171 30 PstI C_TGCA'G Cuts at: 0 21 332 818 1474 1770 2121 Size: 21 311 486 656 296 351 Fragments arranged by size: 656 486 351 311 296 21 PvuII CAG'CTG Cuts at: 0 678 1803 2121 Size: 678 1125 318 Fragments arranged by size: 1125 678 318 SacI G_AGCT'C Cuts at: 0 1103 2121 Size: 1103 1018 SacII CC_GC'GG Cuts at: 0 1386 2121 Size: 1386 735 SanDI GG'GwC_CC Cuts at: 0 30 201 1110 2121 Size: 30 171 909 1011 Fragments arranged by size: 1011 909 171 30 SapI GCTCTTCn'nnn_ Cuts at: 0 1270 1564 2121 Size: 1270 294 557 Fragments arranged by size: 1270 557 294 SbfI CC_TGCA'GG Cuts at: 0 1770 2121 Size: 1770 351 SfcI C'TryA_G Cuts at: 0 17 206 328 648 814 1470 1766 2121 Size: 17 189 122 320 166 656 296 355 Fragments arranged by size: 656 355 320 296 189 166 122 17 SfoI GGC'GCC Cuts at: 0 1969 2121 Size: 1969 152 SmaI CCC'GGG Cuts at: 0 51 736 2121 Size: 51 685 1385 Fragments arranged by size: 1385 685 51 StuI AGG'CCT Cuts at: 0 874 2121 Size: 874 1247 StyI C'CwwG_G Cuts at: 0 501 1742 2121 Size: 501 1241 379 Fragments arranged by size: 1241 501 379 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 745 1414 1635 2055 2121 Size: 745 669 221 420 66 Fragments arranged by size: 745 669 420 221 66 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 386 418 1888 1920 2121 Size: 386 32 1470 32 201 Fragments arranged by size: 1470 386 201 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1365 2121 Size: 1365 756 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 696 717 1041 1713 2121 Size: 696 21 324 672 408 Fragments arranged by size: 696 672 408 324 21 XmaI C'CCGG_G Cuts at: 0 49 734 2121 Size: 49 685 1387 Fragments arranged by size: 1387 685 49 XmnI GAAnn'nnTTC Cuts at: 0 59 2121 Size: 59 2062 Enzymes that do cut: AarI AccI AcuI AlfI AloI AlwNI ApaLI AvaI BamHI BanI BanII BbeI BbsI BbvCI BglI Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BstEII BstXI BstYI Bsu36I BtgI BtsI ClaI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV PasI PflMI PfoI PmlI PpiI PpuMI PstI PvuII SacI SacII SanDI SapI SbfI SfcI SfoI SmaI StuI StyI TaqII TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI ApaI ApoI AscI AseI AsiSI AvrII BaeI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI BpuEI BsiEI BsiWI BspEI BspHI BsrGI BssHII BssSI BstAPI BstBI BstZ17I BtgZI CspCI DraI DraIII EagI EcoRI EcoRV FseI FspAI HincII HindIII HpaI KpnI MfeI MluI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SalI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI XbaI XhoI ZraI