################################## # NO CUT ENZYMES ( 155 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BcgI BclI BfrI BglII BlnI BsaAI BsaBI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I ClaI CpoI DpnII DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HindIII HpaI KasI KpnI KspI MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NaeI NarI NcoI NdeI NdeII NgoMIV NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI Tru9I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 334 GACGTC GACGT^C AccII 533 CGCG CG^CG AlwNI 56 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 234 CCTGAGG CC^T[ACGT]AGG ApaI 415 GGGCCC GGGCC^C BanII 415 GGGCCC G[GA]GC[CT]^C BbsI 214 GTCTTC GAAGAC(2/6) BbvCI 400 CCTCAGC CCTCAGC(-5/-2) BciVI 392 GTATCC GTATCC(6/5) BlpI 277 GCTGAGC GC^T[ACGT]AGC BmyI 415 GGGCCC G[AGT]GC[ACT]^C Bpu1102I 277 GCTGAGC GC^T[ACGT]AGC BpuAI 214 GTCTTC GAAGAC(2/6) BsaI 536 GAGACC GGTCTC(1/5) BseRI 526 CTCCTC GAGGAG(10/8) BsmAI 536 GAGAC GTCTC(1/5) Bsp1286I 415 GGGCCC G[AGT]GC[ACT]^C BspMI 110 GCAGGT ACCTGC(4/8) BsrFI 452 ACCGGC [GA]^CCGG[CT] BstF5I 369 CATCC GGATG(2/0) BstUI 533 CGCG CG^CG Bsu36I 234 CCTGAGG CC^T[ACGT]AGG CelII 277 GCTGAGC GC^T[ACGT]AGC CfoI 258 GCGC GCG^C Cfr10I 452 ACCGGC [GA]^CCGG[CT] Eco81I 234 CCTGAGG CC^T[ACGT]AGG FokI 369 CATCC GGATG(9/13) HaeII 257 AGCGCC [GA]GCGC^[CT] HhaI 258 GCGC GCG^C HinP1I 258 GCGC G^CGC HincII 579 GTCAAC GT[CT]^[GA]AC HindII 579 GTCAAC GT[CT]^[GA]AC MvnI 533 CGCG CG^CG NheI 138 GCTAGC G^CTAGC PstI 5 CTGCAG CTGCA^G SfaNI 499 GCATC GCATC(5/9) SfcI 5 CTGCAG C^T[GA][CT]AG SmlI 181 CTCAAG C^T[CT][GA]AG StuI 386 AGGCCT AGG^CCT Tsp509I 226 AATT ^AATT TspRI 442 CACTG CA[GC]TG[ACGT][ACGT]^ XcmI 158 CCAGGGACGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcyI 163 GACGCC G[GA]^CG[CT]C 334 GACGTC AvaI 220 CCCGGG C^[CT]CG[GA]G 425 CCCGGG BglI 101 GCCACATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 268 GCCTTGGAGGC BsaHI 163 GACGCC G[GA]^CG[CT]C 334 GACGTC BsgI 361 CTGCAC GTGCAG(16/14) 448 CTGCAC BsoBI 220 CCCGGG C^[CT]CG[GA]G 425 CCCGGG DsaI 17 CCGTGG C^C[GA][CT]GG 50 CCGTGG EaeI 47 TGGCCG [CT]^GGCC[GA] 551 TGGCCG EarI 517 CTCTTC CTCTTC(1/4) 172 GAAGAG EcoT14I 167 CCTTGG C^C[AT][AT]GG 269 CCTTGG HgaI 163 GACGC GACGC(5/10) 291 GACGC Hin1I 163 GACGCC G[GA]^CG[CT]C 334 GACGTC Ksp632I 517 CTCTTC CTCTTC(1/4) 172 GAAGAG MaeII 335 ACGT A^CGT 577 ACGT MspA1I 311 CCGCTG C[AC]G^C[GT]G 482 CCGCTG PpuMI 230 GGGACCT [GA]G^G[AT]CC[CT] 299 AGGTCCT SmaI 220 CCCGGG CCC^GGG 425 CCCGGG StyI 167 CCTTGG C^C[AT][AT]GG 269 CCTTGG XmaCI 220 CCCGGG C^CCGGG 425 CCCGGG XmaI 220 CCCGGG C^CCGGG 425 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AciI AluI AvaII BbvI BcnI BfaI BpmI BsaJI BsiYI BslI BsmFI BsrI BssKI BstNI Cac8I Cfr13I DdeI DraII EcoO109I EcoRII Fnu4HI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeIII MboII MnlI MspI MvaI MwoI NciI NlaIV PleI Sau96I ScrFI TseI Tsp45I