(Circular) (Six-base) MAPSORT of: pFN21AE0242.seq Check: 1458 from: 1 to: 6061 pFN21AE0242 6061 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3871 278 Size: 53 83 186 936 2335 2468 Fragments arranged by size: 2468 2335 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3404 1937 Size: 1467 4594 Acc65I G'GTAC_C Cuts at: 3382 3382 Size: 6061 AclI AA'CG_TT Cuts at: 1563 3360 4188 4561 1563 Size: 1797 828 373 3063 Fragments arranged by size: 3063 1797 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2433 2555 2753 4124 5508 5776 1632 Size: 801 122 198 1371 1384 268 1917 Fragments arranged by size: 1917 1384 1371 801 268 198 122 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5864 AflIII A'CryG_T Cuts at: 1203 4960 5937 1203 Size: 3757 977 1327 Fragments arranged by size: 3757 1327 977 AgeI A'CCGG_T Cuts at: 4892 4892 Size: 6061 AhdI GACnn_n'nnGTC Cuts at: 4790 4790 Size: 6061 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6061 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3195 1184 Size: 2011 4050 AlwNI CAG_nnn'CTG Cuts at: 2117 2202 2243 2458 2528 3804 5376 2117 Size: 85 41 215 70 1276 1572 2802 Fragments arranged by size: 2802 1572 1276 215 85 70 41 ApaI G_GGCC'C Cuts at: 2097 2470 2097 Size: 373 5688 ApaLI G'TGCA_C Cuts at: 4118 5274 4118 Size: 1156 4905 ApoI r'AATT_y Cuts at: 1493 3370 3596 3748 5857 1493 Size: 1877 226 152 2109 1697 Fragments arranged by size: 2109 1877 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4615 160 Size: 872 3583 1606 Fragments arranged by size: 3583 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 AvaI C'yCGr_G Cuts at: 1870 1943 2988 3213 3386 1870 Size: 73 1045 225 173 4545 Fragments arranged by size: 4545 1045 225 173 73 BamHI G'GATC_C Cuts at: 2680 3391 3758 2680 Size: 711 367 4983 Fragments arranged by size: 4983 711 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2583 2725 3255 3382 4837 Size: 325 217 627 796 142 530 127 1455 Cuts at: 4837 618 Size: 1842 Fragments arranged by size: 1842 1455 796 627 530 325 217 142 127 BanII G_rGCy'C Cuts at: 1391 2097 2470 2664 2867 3062 3108 3222 3298 Size: 706 373 194 203 195 46 114 76 Cuts at: 3298 3380 1391 Size: 82 4072 Fragments arranged by size: 4072 706 373 203 195 194 114 82 76 46 BbeI G_GCGC'C Cuts at: 2729 2729 Size: 6061 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2379 928 Size: 971 480 4610 Fragments arranged by size: 4610 971 480 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2885 2919 4252 4286 1903 Size: 34 50 34 864 34 1333 34 3678 Fragments arranged by size: 3678 1333 864 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3953 5163 3953 Size: 1210 4851 BclI T'GATC_A Cuts at: 1548 1548 Size: 6061 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2813 4672 136 Size: 107 122 71 631 605 1141 1859 1525 Fragments arranged by size: 1859 1525 1141 631 605 122 107 71 BglII A'GATC_T Cuts at: 6056 6056 Size: 6061 BlpI GC'TnA_GC Cuts at: 2312 3221 3478 2312 Size: 909 257 4895 Fragments arranged by size: 4895 909 257 Bme1580I G_kGCm'C Cuts at: 1790 2097 2470 2839 4122 5278 1790 Size: 307 373 369 1283 1156 2573 Fragments arranged by size: 2573 1283 1156 373 369 307 BmgBI CAC'GTC Cuts at: 1318 2434 2449 1318 Size: 1116 15 4930 Fragments arranged by size: 4930 1116 15 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3792 4750 442 Size: 416 53 83 399 24 2375 958 1753 Fragments arranged by size: 2375 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6061 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2207 2585 2717 3076 4721 1239 Size: 968 378 132 359 1645 2579 Fragments arranged by size: 2579 1645 968 378 359 132 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2208 4186 5051 5349 2208 Size: 1978 865 298 2920 Fragments arranged by size: 2920 1978 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2183 2296 4724 882 Size: 617 684 113 2428 2219 Fragments arranged by size: 2428 2219 684 617 113 BsaAI yAC'GTr Cuts at: 493 1204 2482 3156 3852 493 Size: 711 1278 674 696 2702 Fragments arranged by size: 2702 1278 711 696 674 BsaBI GATnn'nnATC Cuts at: 6055 6055 Size: 6061 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2726 3021 3868 4250 Size: 53 83 186 936 1193 295 847 382 Cuts at: 4250 4885 275 Size: 635 1451 Fragments arranged by size: 1451 1193 936 847 635 382 295 186 83 53 BsaWI w'CCGG_w Cuts at: 2773 2851 4494 4892 5166 5313 5724 2773 Size: 78 1643 398 274 147 411 3110 Fragments arranged by size: 3110 1643 411 398 274 147 78 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2276 2306 1178 Size: 30 165 30 873 30 4933 Fragments arranged by size: 4933 873 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2378 2381 2939 1186 Size: 1192 3 558 4308 Fragments arranged by size: 4308 1192 558 3 BseYI C'CCAG_C Cuts at: 2202 3137 5264 5760 2202 Size: 935 2127 496 2503 Fragments arranged by size: 2503 2127 935 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2141 2983 3155 3230 3257 1163 Size: 573 405 842 172 75 27 3967 Fragments arranged by size: 3967 842 573 405 172 75 27 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3511 4272 5300 1540 Size: 275 186 1510 761 1028 2301 Fragments arranged by size: 2301 1510 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2439 2773 3108 4122 4207 5278 2439 Size: 334 335 1014 85 1071 3222 Fragments arranged by size: 3222 1071 1014 335 334 85 BsmI GAATG_Cn' Cuts at: 3572 3665 3572 Size: 93 5968 BsmBI CGTCTCn'nnnn_ Cuts at: 2693 2693 Size: 6061 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2097 2439 2470 2664 2773 2839 2867 Size: 399 307 342 31 194 109 66 28 Cuts at: 2867 3062 3108 3222 3298 3380 4122 4207 5278 Size: 195 46 114 76 82 742 85 1071 Cuts at: 5278 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 342 307 195 194 114 109 85 82 76 66 46 31 28 BspEI T'CCGG_A Cuts at: 5724 5724 Size: 6061 BspHI T'CATG_A Cuts at: 3950 5680 3950 Size: 1730 4331 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2022 3315 3416 844 Size: 1178 1293 101 3489 Fragments arranged by size: 3489 1293 1178 101 BsrBI CCG'CTC Cuts at: 1394 1823 2311 3948 1394 Size: 429 488 1637 3507 Fragments arranged by size: 3507 1637 488 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3177 4556 4730 65 Size: 1183 1929 1379 174 1396 Fragments arranged by size: 1929 1396 1379 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2338 2737 3031 4705 4892 1700 Size: 110 528 399 294 1674 187 2869 Fragments arranged by size: 2869 1674 528 399 294 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6061 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6061 BssSI C'ACGA_G Cuts at: 2894 4121 5133 2894 Size: 1227 1012 3822 Fragments arranged by size: 3822 1227 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6061 BstBI TT'CG_AA Cuts at: 4865 4865 Size: 6061 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6061 BstXI CCAn_nnnn'nTGG Cuts at: 2356 2546 2356 Size: 190 5871 BstYI r'GATC_y Cuts at: 1972 2113 2680 3004 3112 3391 3758 4143 4160 Size: 141 567 324 108 279 367 385 17 Cuts at: 4160 5612 5698 5710 6056 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 567 385 367 346 324 279 141 108 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2427 2427 Size: 6061 BtgI C'CryG_G Cuts at: 513 1065 2539 2559 3323 513 Size: 552 1474 20 764 3251 Fragments arranged by size: 3251 1474 764 552 20 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2500 487 Size: 683 56 1274 4048 Fragments arranged by size: 4048 1274 683 56 BtsI GCAGTG_nn' Cuts at: 3577 4371 4391 3577 Size: 794 20 5247 Fragments arranged by size: 5247 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6061 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3142 3177 529 Size: 35 2578 35 3413 Fragments arranged by size: 3413 2578 35 35 DraI TTT'AAA Cuts at: 3365 3365 Size: 6061 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5068 809 Size: 507 3752 1802 Fragments arranged by size: 3752 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2484 2556 3508 Size: 54 1425 50 275 18 654 72 952 Cuts at: 3508 4397 6026 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 952 889 654 275 72 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3508 1537 Size: 275 1696 4090 Fragments arranged by size: 4090 1696 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2355 2409 3327 3991 1353 Size: 1002 54 918 664 3423 Fragments arranged by size: 3423 1002 918 664 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3280 4648 5022 5168 387 Size: 1064 138 1691 1368 374 146 1280 Fragments arranged by size: 1691 1368 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3106 3106 Size: 6061 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2207 2433 2555 2585 2717 2753 3076 Size: 393 575 226 122 30 132 36 323 Cuts at: 3076 4124 4721 5508 5776 1239 Size: 1048 597 787 268 1524 Fragments arranged by size: 1524 1048 787 597 575 393 323 268 226 132 122 36 30 EcoNI CCTnn'n_nnAGG Cuts at: 2615 2629 2954 2615 Size: 14 325 5722 Fragments arranged by size: 5722 325 14 EcoO109I rG'GnC_Cy Cuts at: 2093 2423 2466 2534 2551 2654 2687 2825 2093 Size: 330 43 68 17 103 33 138 5329 Fragments arranged by size: 5329 330 138 103 68 43 33 17 EcoRI G'AATT_C Cuts at: 3370 3748 3370 Size: 378 5683 FspI TGC'GCA Cuts at: 2513 2907 4567 2513 Size: 394 1660 4007 Fragments arranged by size: 4007 1660 394 FspAI rTGC'GCAy Cuts at: 2513 2513 Size: 6061 HaeII r_GCGC'y Cuts at: 1678 2729 2902 2948 3038 3170 5208 1678 Size: 1051 173 46 90 132 2038 2531 Fragments arranged by size: 2531 2038 1051 173 132 90 46 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2242 2274 4702 4734 4776 Size: 32 135 32 670 32 2428 32 42 Cuts at: 4776 4808 1373 Size: 32 2626 Fragments arranged by size: 2626 2428 670 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3405 3651 669 Size: 988 281 1467 246 3079 Fragments arranged by size: 3079 1467 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6061 HpaI GTT'AAC Cuts at: 3651 3651 Size: 6061 KasI G'GCGC_C Cuts at: 2725 2725 Size: 6061 KpnI G_GTAC'C Cuts at: 3386 3386 Size: 6061 MfeI C'AATT_G Cuts at: 3660 3660 Size: 6061 MluI A'CGCG_T Cuts at: 5937 5937 Size: 6061 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2161 5175 5359 1305 Size: 856 3014 184 2007 Fragments arranged by size: 3014 2007 856 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2486 10 Size: 54 1425 343 654 3585 Fragments arranged by size: 3585 1425 654 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4019 4378 4537 518 Size: 648 2853 359 159 2042 Fragments arranged by size: 2853 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3082 3477 4154 5302 5547 1606 Size: 1476 395 677 1148 245 2120 Fragments arranged by size: 2120 1476 1148 677 395 245 NaeI GCC'GGC Cuts at: 2340 2739 3033 2340 Size: 399 294 5368 Fragments arranged by size: 5368 399 294 NarI GG'CG_CC Cuts at: 2726 2726 Size: 6061 NcoI C'CATG_G Cuts at: 513 1065 3323 513 Size: 552 2258 3251 Fragments arranged by size: 3251 2258 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6061 NgoMIV G'CCGG_C Cuts at: 2338 2737 3031 2338 Size: 399 294 5368 Fragments arranged by size: 5368 399 294 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6061 NotI GC'GGCC_GC Cuts at: 3508 3508 Size: 6061 NspI r_CATG'y Cuts at: 1132 1229 3419 4964 1132 Size: 97 2190 1545 2229 Fragments arranged by size: 2229 2190 1545 97 PasI CC'CwG_GG Cuts at: 1348 2150 3010 1348 Size: 802 860 4399 Fragments arranged by size: 4399 860 802 PciI A'CATG_T Cuts at: 4960 4960 Size: 6061 PflMI CCAn_nnn'nTGG Cuts at: 1108 2026 2458 1108 Size: 918 432 4711 Fragments arranged by size: 4711 918 432 PfoI T'CCnGG_A Cuts at: 2601 2601 Size: 6061 PmeI GTTT'AAAC Cuts at: 3365 3365 Size: 6061 PmlI CAC'GTG Cuts at: 3156 3156 Size: 6061 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4130 4162 5643 5675 4130 Size: 32 1481 32 4516 Fragments arranged by size: 4516 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2534 2551 2654 2534 Size: 17 103 5941 Fragments arranged by size: 5941 103 17 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6061 PsiI TTA'TAA Cuts at: 3631 3631 Size: 6061 PspOMI G'GGCC_C Cuts at: 2093 2466 2093 Size: 373 5688 PspXI vC'TCGA_Gb Cuts at: 2988 2988 Size: 6061 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2165 2197 2165 Size: 32 6029 PstI C_TGCA'G Cuts at: 830 3413 830 Size: 2583 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6061 PvuII CAG'CTG Cuts at: 3082 3082 Size: 6061 SacI G_AGCT'C Cuts at: 3108 3108 Size: 6061 SalI G'TCGA_C Cuts at: 1936 3403 1936 Size: 1467 4594 SanDI GG'GwC_CC Cuts at: 2534 2551 2534 Size: 17 6044 SbfI CC_TGCA'GG Cuts at: 3413 3413 Size: 6061 ScaI AGT'ACT Cuts at: 4309 6021 4309 Size: 1712 4349 SfcI C'TryA_G Cuts at: 826 1045 3409 4544 5225 5416 826 Size: 219 2364 1135 681 191 1471 Fragments arranged by size: 2364 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2727 2727 Size: 6061 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6061 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6061 SmaI CCC'GGG Cuts at: 1872 3215 3388 1872 Size: 1343 173 4545 Fragments arranged by size: 4545 1343 173 SmlI C'TyrA_G Cuts at: 820 1017 1943 2223 2988 4165 5066 5328 820 Size: 197 926 280 765 1177 901 262 1553 Fragments arranged by size: 1553 1177 926 901 765 280 262 197 SnaBI TAC'GTA Cuts at: 493 3852 493 Size: 3359 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6061 SphI G_CATG'C Cuts at: 1132 3419 1132 Size: 2287 3774 SspI AAT'ATT Cuts at: 5 52 3985 5 Size: 47 3933 2081 Fragments arranged by size: 3933 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6061 StyI C'CwwG_G Cuts at: 513 1065 3323 3500 513 Size: 552 2258 177 3074 Fragments arranged by size: 3074 2258 552 177 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2287 3056 4088 4105 4258 4443 4888 Size: 1255 707 769 1032 17 153 185 445 Cuts at: 4888 325 Size: 1498 Fragments arranged by size: 1498 1255 1032 769 707 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4307 6019 96 Size: 274 80 33 51 1443 2330 1712 138 Fragments arranged by size: 2330 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2276 2308 6021 6053 2276 Size: 32 3713 32 2284 Fragments arranged by size: 3713 2284 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6061 XbaI T'CTAG_A Cuts at: 3397 3397 Size: 6061 XhoI C'TCGA_G Cuts at: 1943 2988 1943 Size: 1045 5016 XmaI C'CCGG_G Cuts at: 1870 3213 3386 1870 Size: 1343 173 4545 Fragments arranged by size: 4545 1343 173 XmnI GAAnn'nnTTC Cuts at: 4190 4190 Size: 6061 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3869 276 Size: 53 83 186 936 2335 2468 Fragments arranged by size: 2468 2335 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoRV FalI FseI NruI NsiI PacI RsrII SacII SapI SexAI SfiI SrfI SwaI XcmI