(Linear) (Six-base) MAPSORT of: KIEE6601.seq Check: 5372 from: 1 to: 705 KIEE6601.orf With 182 enzymes: SgfI * March 16, 2015 11:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 169 705 Size: 169 536 AatII G_ACGT'C Cuts at: 0 431 705 Size: 431 274 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 448 705 Size: 448 257 AleI CACnn'nnGTG Cuts at: 0 346 705 Size: 346 359 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 40 705 Size: 40 665 AlwNI CAG_nnn'CTG Cuts at: 0 105 117 147 705 Size: 105 12 30 558 Fragments arranged by size: 558 105 30 12 AvaI C'yCGr_G Cuts at: 0 246 406 705 Size: 246 160 299 Fragments arranged by size: 299 246 160 BanI G'GyrC_C Cuts at: 0 298 355 544 652 705 Size: 298 57 189 108 53 Fragments arranged by size: 298 189 108 57 53 BanII G_rGCy'C Cuts at: 0 38 554 705 Size: 38 516 151 Fragments arranged by size: 516 151 38 BbeI G_GCGC'C Cuts at: 0 302 359 548 656 705 Size: 302 57 189 108 49 Fragments arranged by size: 302 189 108 57 49 BglI GCCn_nnn'nGGC Cuts at: 0 327 535 705 Size: 327 208 170 Fragments arranged by size: 327 208 170 BlpI GC'TnA_GC Cuts at: 0 385 700 705 Size: 385 315 5 Fragments arranged by size: 385 315 5 Bme1580I G_kGCm'C Cuts at: 0 512 705 Size: 512 193 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 310 469 705 Size: 310 159 236 Fragments arranged by size: 310 236 159 BsaHI Gr'CG_yC Cuts at: 0 299 356 428 545 653 705 Size: 299 57 72 117 108 52 Fragments arranged by size: 299 117 108 72 57 52 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 25 351 570 705 Size: 25 326 219 135 Fragments arranged by size: 326 219 135 25 BseYI C'CCAG_C Cuts at: 0 294 705 Size: 294 411 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 298 705 Size: 298 407 BsiEI CG_ry'CG Cuts at: 0 192 331 587 705 Size: 192 139 256 118 Fragments arranged by size: 256 192 139 118 BsiHKAI G_wGCw'C Cuts at: 0 391 554 705 Size: 391 163 151 Fragments arranged by size: 391 163 151 Bsp1286I G_dGCh'C Cuts at: 0 38 391 512 554 705 Size: 38 353 121 42 151 Fragments arranged by size: 353 151 121 42 38 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 113 169 269 446 705 Size: 113 56 100 177 259 Fragments arranged by size: 259 177 113 100 56 BsrBI CCG'CTC Cuts at: 0 228 705 Size: 228 477 BsrFI r'CCGG_y Cuts at: 0 571 705 Size: 571 134 BssHII G'CGCG_C Cuts at: 0 213 284 376 378 380 705 Size: 213 71 92 2 2 325 Fragments arranged by size: 325 213 92 71 2 2 BstAPI GCAn_nnn'nTGC Cuts at: 0 105 117 705 Size: 105 12 588 Fragments arranged by size: 588 105 12 BtgI C'CryG_G Cuts at: 0 322 560 705 Size: 322 238 145 Fragments arranged by size: 322 238 145 DraIII CAC_nnn'GTG Cuts at: 0 69 705 Size: 69 636 DrdI GACnn_nn'nnGTC Cuts at: 0 427 705 Size: 427 278 EaeI y'GGCC_r Cuts at: 0 200 328 635 705 Size: 200 128 307 70 Fragments arranged by size: 307 200 128 70 EagI C'GGCC_G Cuts at: 0 328 705 Size: 328 377 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 106 427 705 Size: 106 321 278 Fragments arranged by size: 321 278 106 EcoICRI GAG'CTC Cuts at: 0 552 705 Size: 552 153 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 310 448 469 705 Size: 310 138 21 236 Fragments arranged by size: 310 236 138 21 EcoNI CCTnn'n_nnAGG Cuts at: 0 109 705 Size: 109 596 EcoO109I rG'GnC_Cy Cuts at: 0 195 459 630 705 Size: 195 264 171 75 Fragments arranged by size: 264 195 171 75 FspI TGC'GCA Cuts at: 0 340 705 Size: 340 365 FspAI rTGC'GCAy Cuts at: 0 340 705 Size: 340 365 HaeII r_GCGC'y Cuts at: 0 25 302 359 548 572 656 705 Size: 25 277 57 189 24 84 49 Fragments arranged by size: 277 189 84 57 49 25 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 541 573 705 Size: 541 32 132 Fragments arranged by size: 541 132 32 HincII GTy'rAC Cuts at: 0 504 705 Size: 504 201 KasI G'GCGC_C Cuts at: 0 298 355 544 652 705 Size: 298 57 189 108 53 Fragments arranged by size: 298 189 108 57 53 MslI CAynn'nnrTG Cuts at: 0 346 493 705 Size: 346 147 212 Fragments arranged by size: 346 212 147 MspA1I CmG'CkG Cuts at: 0 312 324 333 515 705 Size: 312 12 9 182 190 Fragments arranged by size: 312 190 182 12 9 NaeI GCC'GGC Cuts at: 0 573 705 Size: 573 132 NarI GG'CG_CC Cuts at: 0 299 356 545 653 705 Size: 299 57 189 108 52 Fragments arranged by size: 299 189 108 57 52 NgoMIV G'CCGG_C Cuts at: 0 571 705 Size: 571 134 NotI GC'GGCC_GC Cuts at: 0 328 705 Size: 328 377 NspI r_CATG'y Cuts at: 0 279 705 Size: 279 426 PflMI CCAn_nnn'nTGG Cuts at: 0 66 705 Size: 66 639 PpuMI rG'GwC_Cy Cuts at: 0 459 705 Size: 459 246 PspXI vC'TCGA_Gb Cuts at: 0 406 705 Size: 406 299 SacI G_AGCT'C Cuts at: 0 554 705 Size: 554 151 SacII CC_GC'GG Cuts at: 0 325 705 Size: 325 380 SexAI A'CCwGG_T Cuts at: 0 65 705 Size: 65 640 SfoI GGC'GCC Cuts at: 0 300 357 546 654 705 Size: 300 57 189 108 51 Fragments arranged by size: 300 189 108 57 51 SmlI C'TyrA_G Cuts at: 0 406 705 Size: 406 299 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 225 573 705 Size: 225 348 132 Fragments arranged by size: 348 225 132 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 630 705 Size: 630 75 XhoI C'TCGA_G Cuts at: 0 406 705 Size: 406 299 ZraI GAC'GTC Cuts at: 0 429 705 Size: 429 276 Enzymes that do cut: AarI AatII AcuI AleI AlfI AlwNI AvaI BanI BanII BbeI BglI BlpI Bme1580I BpmI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BtgI DraIII DrdI EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI FspAI HaeII Hin4I HincII KasI MslI MspA1I NaeI NarI NgoMIV NotI NspI PflMI PpuMI PspXI SacI SacII SexAI SfoI SmlI TaqII XcmI XhoI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI EarI EcoRI EcoRV FalI FseI HindIII HpaI KpnI MfeI MluI MmeI MscI NcoI NdeI NheI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TatI TstI Tth111I XbaI XmaI XmnI