################################## # NO CUT ENZYMES ( 96 enzymes) # ################################## AclI AcsI AflII AgeI Alw44I AlwNI AocI ApaLI ApoI AscI AseI AsnI AviII BbeI BbrPI BbvCI BciVI BclI BfrI BglII BlpI Bpu1102I BsaBI BseRI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BsrGI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KasI MamI MfeI MseI MunI NarI NcoI NdeI NheI NotI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI Tru9I Tsp509I XbaI XhoI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 280 GACGTC GACGT^C Acc65I 1068 GGTACC G^GTACC AccI 1441 GTAGAC GT^[AC][GT]AC Aor51HI 578 AGCGCT AGC^GCT ApaI 2004 GGGCCC GGGCC^C Asp700I 1773 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1068 GGTACC G^GTACC AspHI 1558 GTGCTC G[AT]GC[AT]^C AvrII 1063 CCTAGG C^CTAGG BalI 1287 TGGCCA TGG^CCA BlnI 1063 CCTAGG C^CTAGG BpmI 1719 CTCCAG CTGGAG(16/14) BsaAI 1729 TACGTG [CT]AC^GT[GA] BsiHKAI 1558 GTGCTC G[AT]GC[AT]^C BsrBI 219 GAGCGG CCGCTC(-3/-3) BsrDI 2123 GCAATG GCAATG(2/0) BssSI 613 CACGAG CACGAG(-5/-1) BstAPI 36 GCAGGACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1547 CCAACTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EagI 996 CGGCCG C^GGCCG EarI 1137 GAAGAG CTCTTC(1/4) EclXI 996 CGGCCG C^GGCCG Eco47III 578 AGCGCT AGC^GCT Eco52I 996 CGGCCG C^GGCCG HincII 283 GTCAAC GT[CT]^[GA]AC HindII 283 GTCAAC GT[CT]^[GA]AC KpnI 1068 GGTACC GGTAC^C Ksp632I 1137 GAAGAG CTCTTC(1/4) KspI 245 CCGCGG CCGC^GG MluI 110 ACGCGT A^CGCGT MluNI 1287 TGGCCA TGG^CCA MscI 1287 TGGCCA TGG^CCA NaeI 679 GCCGGC GCC^GGC NgoMIV 679 GCCGGC G^CCGGC NruI 702 TCGCGA TCG^CGA NspI 618 GCATGC [GA]CATG^[CT] PflMI 390 CCAGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacII 245 CCGCGG CCGC^GG SapI 1137 GAAGAGC GCTCTTC(1/4) SmlI 1810 CTCAAG C^T[CT][GA]AG SphI 618 GCATGC GCATG^C StuI 1605 AGGCCT AGG^CCT TfiI 1994 GATTC G^A[AT]TC Van91I 390 CCAGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1721 CCAGCTTCTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1773 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccIII 840 TCCGGA T^CCGGA 1461 TCCGGA BamHI 689 GGATCC G^GATCC 1037 GGATCC BanII 504 GGGCTC G[GA]GC[CT]^C 2004 GGGCCC BbsI 1824 GTCTTC GAAGAC(2/6) 2071 GTCTTC BcgI 837 CGATCCGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1419 CGACTTTGATGC BpuAI 1824 GTCTTC GAAGAC(2/6) 2071 GTCTTC BsaI 454 GAGACC GGTCTC(1/5) 2149 GAGACC BseAI 840 TCCGGA T^CCGGA 1461 TCCGGA BsgI 273 CTGCAC GTGCAG(16/14) 1582 GTGCAG BsmBI 827 GAGACG CGTCTC(1/5) 1214 GAGACG BspEI 840 TCCGGA T^CCGGA 1461 TCCGGA BstYI 689 GGATCC [GA]^GATC[CT] 1037 GGATCC DsaI 245 CCGCGG C^C[GA][CT]GG 1247 CCACGG EcoT14I 413 CCAAGG C^C[AT][AT]GG 1063 CCTAGG MflI 689 GGATCC [GA]^GATC[CT] 1037 GGATCC MroI 840 TCCGGA T^CCGGA 1461 TCCGGA PpuMI 860 GGGTCCC [GA]G^G[AT]CC[CT] 1907 AGGACCC PvuII 1846 CAGCTG CAG^CTG 2158 CAGCTG StyI 413 CCAAGG C^C[AT][AT]GG 1063 CCTAGG XhoII 689 GGATCC [GA]^GATC[CT] 1037 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 96 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AhdI AluI AlwI AspEI AspI AvaI AvaII BanI BbvI BcnI BfaI BglI BmyI BsaHI BsaJI BsaWI BsiEI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssHII BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI Eam1105I EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflFI PleI PshAI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI TaqI TseI Tsp45I TspRI Tth111I TthHB8I XmaCI XmaI