################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AfaI AflII AgeI AhdI AocI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BbrPI BbsI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaBI BsaI BseAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstF5I BstPI BstZ17I Bsu36I ClaI CpoI DraI DraII DraIII DrdI Eam1105I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HincII HindII HindIII HpaI KpnI MaeI MamI MfeI MluI MroI MseI MunI NcoI NdeI NheI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I RcaI RsaI RsrII SalI SbfI ScaI SexAI SfaNI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 73 GTCTAC GT^[AC][GT]AC AcyI 569 GGCGCC G[GA]^CG[CT]C Alw44I 535 GTGCAC G^TGCAC AlwNI 936 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 419 AGCGCT AGC^GCT ApaI 548 GGGCCC GGGCC^C ApaLI 535 GTGCAC G^TGCAC AvaI 927 CCCGAG C^[CT]CG[GA]G BamHI 1005 GGATCC G^GATCC BbeI 569 GGCGCC GGCGC^C BbvCI 843 GCTGAGG CCTCAGC(-5/-2) BcgI 312 GCAGGCACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 981 GCTGAGC GC^T[ACGT]AGC Bpu1102I 981 GCTGAGC GC^T[ACGT]AGC BsaHI 569 GGCGCC G[GA]^CG[CT]C BsaWI 94 ACCGGA [AT]^CCGG[AT] BseRI 1140 CTCCTC GAGGAG(10/8) BsgI 802 CTGCAC GTGCAG(16/14) BsoBI 927 CCCGAG C^[CT]CG[GA]G BspLU11I 476 ACATGT A^CATGT BsrBI 84 GAGCGG CCGCTC(-3/-3) BsrI 719 ACTGG ACTGG(1/-1) BssHII 661 GCGCGC G^CGCGC BstAPI 537 GCACCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 1005 GGATCC [GA]^GATC[CT] CelII 981 GCTGAGC GC^T[ACGT]AGC EarI 1063 CTCTTC CTCTTC(1/4) Eco47III 419 AGCGCT AGC^GCT Hin1I 569 GGCGCC G[GA]^CG[CT]C HinfI 584 GACTC G^A[ACGT]TC KasI 569 GGCGCC G^GCGCC Ksp632I 1063 CTCTTC CTCTTC(1/4) MflI 1005 GGATCC [GA]^GATC[CT] NarI 569 GGCGCC GG^CGCC NotI 610 GCGGCCGC GC^GGCCGC NruI 353 TCGCGA TCG^CGA NspI 476 ACATGT [GA]CATG^[CT] PflMI 935 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 584 GACTC GAGTC(4/5) PstI 637 CTGCAG CTGCA^G PvuI 669 CGATCG CGAT^CG PvuII 996 CAGCTG CAG^CTG SacI 184 GAGCTC GAGCT^C SapI 1062 GCTCTTC GCTCTTC(1/4) SfcI 637 CTGCAG C^T[GA][CT]AG SfiI 1092 GGCCTCCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 569 GGCGCC GGC^GCC Van91I 935 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 1005 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AflIII 476 ACATGT A^C[GA][CT]GT 1025 ACGTGT AvaII 258 GGTCC G^G[AT]CC 924 GGACC BpmI 1150 CTCCAG CTGGAG(16/14) 181 CTGGAG BsaAI 964 TACGTG [CT]AC^GT[GA] 1024 TACGTG BsmFI 1043 GGGAC GGGAC(10/14) 1082 GGGAC EcoT14I 579 CCTTGG C^C[AT][AT]GG 1097 CCAAGG HphI 103 GGTGA GGTGA(8/7) 734 TCACC KspI 138 CCGCGG CCGC^GG 391 CCGCGG MaeII 965 ACGT A^CGT 1025 ACGT MaeIII 173 GTGAC ^GT[ACGT]AC 473 GTCAC MboII 884 TCTTC GAAGA(8/7) 1064 TCTTC MslI 406 CACCTGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 475 CACATGTATG SacII 138 CCGCGG CCGC^GG 391 CCGCGG StuI 710 AGGCCT AGG^CCT 1100 AGGCCT StyI 579 CCTTGG C^C[AT][AT]GG 1097 CCAAGG Tsp45I 173 GTGAC ^GT[GC]AC 473 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AccII AciI AluI AlwI AspHI BalI BanI BanII BbvI BcnI BglI BmyI BsaJI BsiEI BsiHKAI BsiYI BslI Bsp1286I BspMI BsrFI BssKI BstNI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DsaI EaeI EagI EclXI Eco52I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII ItaI MboI MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV Sau3AI Sau96I ScrFI TseI TspRI