(Circular) (Six-base) MAPSORT of: pFN21AE9560.seq Check: 1392 from: 1 to: 5860 pFN21AE9560 5860 bp With 182 enzymes: SgfI * March 28, 2012 12:07 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2421 2750 2421 Size: 329 5531 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3670 278 Size: 53 83 186 936 2134 2468 Fragments arranged by size: 2468 2134 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2079 3203 1937 Size: 142 1124 4594 Fragments arranged by size: 4594 1124 142 Acc65I G'GTAC_C Cuts at: 3181 3181 Size: 5860 AclI AA'CG_TT Cuts at: 1563 3987 4360 1563 Size: 2424 373 3063 Fragments arranged by size: 3063 2424 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3923 5307 5575 1632 Size: 2291 1384 268 1917 Fragments arranged by size: 2291 1917 1384 268 AfeI AGC'GCT Cuts at: 2426 2426 Size: 5860 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5663 AflIII A'CryG_T Cuts at: 1203 2481 3030 4759 5736 1203 Size: 1278 549 1729 977 1327 Fragments arranged by size: 1729 1327 1278 977 549 AgeI A'CCGG_T Cuts at: 4691 4691 Size: 5860 AhdI GACnn_n'nnGTC Cuts at: 4589 4589 Size: 5860 AleI CACnn'nnGTG Cuts at: 1166 2415 1166 Size: 1249 4611 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2924 1184 Size: 1740 4120 AlwNI CAG_nnn'CTG Cuts at: 2946 3603 5175 2946 Size: 657 1572 3631 Fragments arranged by size: 3631 1572 657 ApaI G_GGCC'C Cuts at: 2557 2557 Size: 5860 ApaLI G'TGCA_C Cuts at: 2540 3917 5073 2540 Size: 1377 1156 3327 Fragments arranged by size: 3327 1377 1156 ApoI r'AATT_y Cuts at: 1493 3169 3395 3547 5656 1493 Size: 1676 226 152 2109 1697 Fragments arranged by size: 2109 1697 1676 226 152 AseI AT'TA_AT Cuts at: 160 1032 4414 160 Size: 872 3382 1606 Fragments arranged by size: 3382 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 AvaI C'yCGr_G Cuts at: 1870 1943 2932 3185 1870 Size: 73 989 253 4545 Fragments arranged by size: 4545 989 253 73 BamHI G'GATC_C Cuts at: 3010 3190 3557 3010 Size: 180 367 5313 Fragments arranged by size: 5313 367 180 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2060 2227 2242 2320 2574 Size: 325 217 627 273 167 15 78 254 Cuts at: 2574 2963 3137 3181 4636 618 Size: 389 174 44 1455 1842 Fragments arranged by size: 1842 1455 627 389 325 273 254 217 174 167 78 44 15 BanII G_rGCy'C Cuts at: 1391 2193 2557 2939 3069 3179 1391 Size: 802 364 382 130 110 4072 Fragments arranged by size: 4072 802 382 364 130 110 BbeI G_GCGC'C Cuts at: 2578 2578 Size: 5860 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4889 BbvCI CC'TCA_GC Cuts at: 2849 2849 Size: 5860 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2306 2340 4051 4085 1903 Size: 34 369 34 1711 34 3678 Fragments arranged by size: 3678 1711 369 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3752 4962 3752 Size: 1210 4650 BclI T'GATC_A Cuts at: 1548 1548 Size: 5860 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2055 2236 2318 Size: 107 122 71 631 605 383 181 82 Cuts at: 2318 2624 3104 4471 136 Size: 306 480 1367 1525 Fragments arranged by size: 1525 1367 631 605 480 383 306 181 122 107 82 71 BglII A'GATC_T Cuts at: 5855 5855 Size: 5860 BlpI GC'TnA_GC Cuts at: 2987 3277 2987 Size: 290 5570 Bme1580I G_kGCm'C Cuts at: 1790 2212 2232 2247 2544 2557 2898 2976 3140 Size: 422 20 15 297 13 341 78 164 Cuts at: 3140 3921 5077 1790 Size: 781 1156 2573 Fragments arranged by size: 2573 1156 781 422 341 297 164 78 20 15 13 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5860 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3591 4549 442 Size: 416 53 83 399 24 2174 958 1753 Fragments arranged by size: 2174 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5860 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2207 3140 4520 1239 Size: 968 933 1380 2579 Fragments arranged by size: 2579 1380 968 933 Bpu10I CC'TnA_GC Cuts at: 2849 2849 Size: 5860 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3985 4850 5148 3985 Size: 865 298 4697 Fragments arranged by size: 4697 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4523 882 Size: 617 3024 2219 Fragments arranged by size: 3024 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2971 3031 3651 493 Size: 711 1767 60 620 2702 Fragments arranged by size: 2702 1767 711 620 60 BsaBI GATnn'nnATC Cuts at: 5854 5854 Size: 5860 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2575 3667 4049 4684 Size: 53 83 186 936 1042 1092 382 635 Cuts at: 4684 275 Size: 1451 Fragments arranged by size: 1451 1092 1042 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2099 4293 4691 4965 5112 5523 2099 Size: 2194 398 274 147 411 2436 Fragments arranged by size: 2436 2194 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5635 Fragments arranged by size: 5635 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3136 1186 Size: 1950 3910 BseYI C'CCAG_C Cuts at: 2038 2072 2223 2897 2939 2975 5063 5559 2038 Size: 34 151 674 42 36 2088 496 2339 Fragments arranged by size: 2339 2088 674 496 151 42 36 34 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2792 1163 Size: 573 1056 4231 Fragments arranged by size: 4231 1056 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2466 2619 2677 2830 3039 3080 Size: 275 186 465 153 58 153 209 41 Cuts at: 3080 3310 4071 5099 1540 Size: 230 761 1028 2301 Fragments arranged by size: 2301 1028 761 465 275 230 209 186 153 153 58 41 BsiHKAI G_wGCw'C Cuts at: 2099 2193 2544 2685 2993 3921 4006 5077 2099 Size: 94 351 141 308 928 85 1071 2882 Fragments arranged by size: 2882 1071 928 351 308 141 94 85 BsmI GAATG_Cn' Cuts at: 3371 3464 3371 Size: 93 5767 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2099 2193 2212 2232 2247 2544 2557 Size: 399 309 94 19 20 15 297 13 Cuts at: 2557 2685 2898 2939 2976 2993 3069 3140 3179 Size: 128 213 41 37 17 76 71 39 Cuts at: 3179 3921 4006 5077 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 309 297 213 128 94 85 76 71 41 39 37 20 19 17 15 13 BspEI T'CCGG_A Cuts at: 5523 5523 Size: 5860 BspHI T'CATG_A Cuts at: 3749 5479 3749 Size: 1730 4130 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2421 2750 2913 3215 844 Size: 1577 329 163 302 3489 Fragments arranged by size: 3489 1577 329 302 163 BsrBI CCG'CTC Cuts at: 1394 1823 2091 3747 1394 Size: 429 268 1656 3507 Fragments arranged by size: 3507 1656 429 268 BsrDI GCAATG_nn' Cuts at: 65 1248 4355 4529 65 Size: 1183 3107 174 1396 Fragments arranged by size: 3107 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2281 2571 2731 2829 4504 4691 1700 Size: 110 471 290 160 98 1675 187 2869 Fragments arranged by size: 2869 1675 471 290 187 160 110 98 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5860 BssHII G'CGCG_C Cuts at: 1925 2666 1925 Size: 741 5119 BssSI C'ACGA_G Cuts at: 3920 4932 3920 Size: 1012 4848 BstAPI GCAn_nnn'nTGC Cuts at: 794 2548 794 Size: 1754 4106 BstBI TT'CG_AA Cuts at: 4664 4664 Size: 5860 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5860 BstXI CCAn_nnnn'nTGG Cuts at: 2239 2293 2417 2239 Size: 54 124 5682 Fragments arranged by size: 5682 124 54 BstYI r'GATC_y Cuts at: 1972 3010 3190 3557 3942 3959 5411 5497 5509 Size: 1038 180 367 385 17 1452 86 12 Cuts at: 5509 5855 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1038 385 367 346 180 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2143 2396 2460 2880 3093 513 Size: 552 1078 253 64 420 213 3280 Fragments arranged by size: 3280 1078 552 420 253 213 64 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5121 Fragments arranged by size: 5121 683 56 BtsI GCAGTG_nn' Cuts at: 2210 2627 2639 2868 3376 4170 4190 2210 Size: 417 12 229 508 794 20 3880 Fragments arranged by size: 3880 794 508 417 229 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5860 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5825 DraI TTT'AAA Cuts at: 3164 3164 Size: 5860 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4867 809 Size: 507 3551 1802 Fragments arranged by size: 3551 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2283 2310 2419 Size: 54 1425 50 275 18 453 27 109 Cuts at: 2419 2457 2463 2505 2569 2616 2778 2820 2827 Size: 38 6 42 64 47 162 42 7 Cuts at: 2827 2839 3036 3307 4196 5825 8 Size: 12 197 271 889 1629 43 Fragments arranged by size: 1629 1425 889 453 275 271 197 162 109 64 54 50 47 43 42 42 38 27 18 12 7 6 EagI C'GGCC_G Cuts at: 1537 1812 2463 2616 2827 3036 3307 1537 Size: 275 651 153 211 209 271 4090 Fragments arranged by size: 4090 651 275 271 211 209 153 EarI CTCTTCn'nnn_ Cuts at: 1353 3074 3790 1353 Size: 1721 716 3423 Fragments arranged by size: 3423 1721 716 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2866 4447 4821 4967 387 Size: 1064 138 1277 1581 374 146 1280 Fragments arranged by size: 1581 1280 1277 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2191 2191 Size: 5860 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2207 3140 3923 4520 5307 5575 1239 Size: 393 575 933 783 597 787 268 1524 Fragments arranged by size: 1524 933 787 783 597 575 393 268 EcoRI G'AATT_C Cuts at: 3169 3547 3169 Size: 378 5482 FspI TGC'GCA Cuts at: 4366 4366 Size: 5860 HaeII r_GCGC'y Cuts at: 1678 2300 2390 2428 2578 2753 2836 2946 2987 Size: 622 90 38 150 175 83 110 41 Cuts at: 2987 5007 1678 Size: 2020 2531 Fragments arranged by size: 2531 2020 622 175 150 110 90 83 41 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4501 4533 4575 4607 1373 Size: 32 135 32 2929 32 42 32 2626 Fragments arranged by size: 2929 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3204 3450 669 Size: 988 281 1266 246 3079 Fragments arranged by size: 3079 1266 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5860 HpaI GTT'AAC Cuts at: 3450 3450 Size: 5860 KasI G'GCGC_C Cuts at: 2574 2574 Size: 5860 KpnI G_GTAC'C Cuts at: 3185 3185 Size: 5860 MfeI C'AATT_G Cuts at: 3459 3459 Size: 5860 MluI A'CGCG_T Cuts at: 5736 5736 Size: 5860 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3097 4974 5158 1305 Size: 1792 1877 184 2007 Fragments arranged by size: 2007 1877 1792 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2312 2421 2459 2780 10 Size: 54 1425 343 480 109 38 321 3090 Fragments arranged by size: 3090 1425 480 343 321 109 54 38 MslI CAynn'nnrTG Cuts at: 518 1166 2415 2484 3818 4177 4336 518 Size: 648 1249 69 1334 359 159 2042 Fragments arranged by size: 2042 1334 1249 648 359 159 69 MspA1I CmG'CkG Cuts at: 1606 2145 2398 2548 2647 2826 3003 3276 3953 Size: 539 253 150 99 179 177 273 677 Cuts at: 3953 5101 5346 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 539 273 253 245 179 177 150 99 NaeI GCC'GGC Cuts at: 2283 2573 2831 2283 Size: 290 258 5312 Fragments arranged by size: 5312 290 258 NarI GG'CG_CC Cuts at: 2575 2575 Size: 5860 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5308 NdeI CA'TA_TG Cuts at: 387 387 Size: 5860 NgoMIV G'CCGG_C Cuts at: 2281 2571 2829 2281 Size: 290 258 5312 Fragments arranged by size: 5312 290 258 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5860 NotI GC'GGCC_GC Cuts at: 2616 3307 2616 Size: 691 5169 NruI TCG'CGA Cuts at: 2360 2360 Size: 5860 NspI r_CATG'y Cuts at: 1132 1229 2485 3218 4763 1132 Size: 97 1256 733 1545 2229 Fragments arranged by size: 2229 1545 1256 733 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5860 PciI A'CATG_T Cuts at: 2481 4759 2481 Size: 2278 3582 PflMI CCAn_nnn'nTGG Cuts at: 1108 2946 1108 Size: 1838 4022 PfoI T'CCnGG_A Cuts at: 2193 2193 Size: 5860 PmeI GTTT'AAAC Cuts at: 3164 3164 Size: 5860 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3929 3961 5442 5474 3929 Size: 32 1481 32 4315 Fragments arranged by size: 4315 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5860 PsiI TTA'TAA Cuts at: 3430 3430 Size: 5860 PspOMI G'GGCC_C Cuts at: 2553 2553 Size: 5860 PstI C_TGCA'G Cuts at: 830 2646 3212 830 Size: 1816 566 3478 Fragments arranged by size: 3478 1816 566 PvuI CG_AT'CG Cuts at: 2001 2677 2001 Size: 676 5184 PvuII CAG'CTG Cuts at: 3003 3003 Size: 5860 SacI G_AGCT'C Cuts at: 2193 2193 Size: 5860 SacII CC_GC'GG Cuts at: 2146 2399 2146 Size: 253 5607 SalI G'TCGA_C Cuts at: 1936 3202 1936 Size: 1266 4594 SapI GCTCTTCn'nnn_ Cuts at: 3074 3074 Size: 5860 SbfI CC_TGCA'GG Cuts at: 3212 3212 Size: 5860 ScaI AGT'ACT Cuts at: 4108 5820 4108 Size: 1712 4148 SfcI C'TryA_G Cuts at: 826 1045 2642 3208 4343 5024 5215 826 Size: 219 1597 566 1135 681 191 1471 Fragments arranged by size: 1597 1471 1135 681 566 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3104 3104 Size: 5860 SfoI GGC'GCC Cuts at: 2576 2576 Size: 5860 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5860 SmaI CCC'GGG Cuts at: 1872 3187 1872 Size: 1315 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3964 4865 5127 820 Size: 197 926 2021 901 262 1553 Fragments arranged by size: 2021 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3651 493 Size: 3158 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5860 SphI G_CATG'C Cuts at: 1132 3218 1132 Size: 2086 3774 SspI AAT'ATT Cuts at: 5 52 3784 5 Size: 47 3732 2081 Fragments arranged by size: 3732 2081 47 StuI AGG'CCT Cuts at: 1517 2717 3107 1517 Size: 1200 390 4270 Fragments arranged by size: 4270 1200 390 StyI C'CwwG_G Cuts at: 513 1065 2584 3102 3299 513 Size: 552 1519 518 197 3074 Fragments arranged by size: 3074 1519 552 518 197 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2078 2215 2293 3155 3887 3904 4057 Size: 1255 498 137 78 862 732 17 153 Cuts at: 4057 4242 4687 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 862 732 498 445 185 153 137 78 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4106 5818 96 Size: 274 80 33 51 1443 2129 1712 138 Fragments arranged by size: 2129 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5820 5852 5820 Size: 32 5828 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5860 XbaI T'CTAG_A Cuts at: 3196 3196 Size: 5860 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5860 XmaI C'CCGG_G Cuts at: 1870 3185 1870 Size: 1315 4545 XmnI GAAnn'nnTTC Cuts at: 3989 3989 Size: 5860 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3668 276 Size: 53 83 186 936 2134 2468 Fragments arranged by size: 2468 2134 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SanDI SexAI SrfI SwaI XcmI