(Linear) (Six-base) MAPSORT of: KIEE9560.seq Check: 2441 from: 1 to: 1155 KIEE9560.orf With 182 enzymes: SgfI * March 22, 2012 18:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 416 745 1155 Size: 416 329 410 Fragments arranged by size: 416 410 329 AccI GT'mk_AC Cuts at: 0 74 1155 Size: 74 1081 AfeI AGC'GCT Cuts at: 0 421 1155 Size: 421 734 AflIII A'CryG_T Cuts at: 0 476 1025 1155 Size: 476 549 130 Fragments arranged by size: 549 476 130 AleI CACnn'nnGTG Cuts at: 0 410 1155 Size: 410 745 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 919 1155 Size: 919 236 AlwNI CAG_nnn'CTG Cuts at: 0 941 1155 Size: 941 214 ApaI G_GGCC'C Cuts at: 0 552 1155 Size: 552 603 ApaLI G'TGCA_C Cuts at: 0 535 1155 Size: 535 620 AvaI C'yCGr_G Cuts at: 0 927 1155 Size: 927 228 BamHI G'GATC_C Cuts at: 0 1005 1155 Size: 1005 150 BanI G'GyrC_C Cuts at: 0 55 222 237 315 569 958 1132 1155 Size: 55 167 15 78 254 389 174 23 Fragments arranged by size: 389 254 174 167 78 55 23 15 BanII G_rGCy'C Cuts at: 0 188 552 934 1064 1155 Size: 188 364 382 130 91 Fragments arranged by size: 382 364 188 130 91 BbeI G_GCGC'C Cuts at: 0 573 1155 Size: 573 582 BbvCI CC'TCA_GC Cuts at: 0 844 1155 Size: 844 311 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 301 335 1155 Size: 301 34 820 Fragments arranged by size: 820 301 34 BglI GCCn_nnn'nGGC Cuts at: 0 50 231 313 619 1099 1155 Size: 50 181 82 306 480 56 Fragments arranged by size: 480 306 181 82 56 50 BlpI GC'TnA_GC Cuts at: 0 982 1155 Size: 982 173 Bme1580I G_kGCm'C Cuts at: 0 207 227 242 539 552 893 971 1135 Size: 207 20 15 297 13 341 78 164 Cuts at: 1135 1155 Size: 20 Fragments arranged by size: 341 297 207 164 78 20 20 15 13 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 202 1135 1155 Size: 202 933 20 Fragments arranged by size: 933 202 20 Bpu10I CC'TnA_GC Cuts at: 0 844 1155 Size: 844 311 BsaAI yAC'GTr Cuts at: 0 966 1026 1155 Size: 966 60 129 Fragments arranged by size: 966 129 60 BsaHI Gr'CG_yC Cuts at: 0 570 1155 Size: 570 585 BsaWI w'CCGG_w Cuts at: 0 94 1155 Size: 94 1061 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1131 1155 Size: 1131 24 BseYI C'CCAG_C Cuts at: 0 33 67 218 892 934 970 1155 Size: 33 34 151 674 42 36 185 Fragments arranged by size: 674 185 151 42 36 34 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 787 1155 Size: 787 368 BsiEI CG_ry'CG Cuts at: 0 461 614 672 825 1034 1075 1155 Size: 461 153 58 153 209 41 80 Fragments arranged by size: 461 209 153 153 80 58 41 BsiHKAI G_wGCw'C Cuts at: 0 94 188 539 680 988 1155 Size: 94 94 351 141 308 167 Fragments arranged by size: 351 308 167 141 94 94 Bsp1286I G_dGCh'C Cuts at: 0 94 188 207 227 242 539 552 680 Size: 94 94 19 20 15 297 13 128 Cuts at: 680 893 934 971 988 1064 1135 1155 Size: 213 41 37 17 76 71 20 Fragments arranged by size: 297 213 128 94 94 76 71 41 37 20 20 19 17 15 13 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 416 745 908 1155 Size: 416 329 163 247 Fragments arranged by size: 416 329 247 163 BsrBI CCG'CTC Cuts at: 0 86 1155 Size: 86 1069 BsrFI r'CCGG_y Cuts at: 0 276 566 726 824 1155 Size: 276 290 160 98 331 Fragments arranged by size: 331 290 276 160 98 BssHII G'CGCG_C Cuts at: 0 661 1155 Size: 661 494 BstAPI GCAn_nnn'nTGC Cuts at: 0 543 1155 Size: 543 612 BstXI CCAn_nnnn'nTGG Cuts at: 0 234 288 412 1155 Size: 234 54 124 743 Fragments arranged by size: 743 234 124 54 BstYI r'GATC_y Cuts at: 0 1005 1155 Size: 1005 150 BtgI C'CryG_G Cuts at: 0 138 391 455 875 1088 1155 Size: 138 253 64 420 213 67 Fragments arranged by size: 420 253 213 138 67 64 BtsI GCAGTG_nn' Cuts at: 0 205 622 634 863 1155 Size: 205 417 12 229 292 Fragments arranged by size: 417 292 229 205 12 EaeI y'GGCC_r Cuts at: 0 278 305 414 452 458 500 564 611 Size: 278 27 109 38 6 42 64 47 Cuts at: 611 773 815 822 834 1031 1155 Size: 162 42 7 12 197 124 Fragments arranged by size: 278 197 162 124 109 64 47 42 42 38 27 12 7 6 EagI C'GGCC_G Cuts at: 0 458 611 822 1031 1155 Size: 458 153 211 209 124 Fragments arranged by size: 458 211 209 153 124 EarI CTCTTCn'nnn_ Cuts at: 0 1069 1155 Size: 1069 86 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 861 1155 Size: 861 294 EcoICRI GAG'CTC Cuts at: 0 186 1155 Size: 186 969 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 202 1135 1155 Size: 202 933 20 Fragments arranged by size: 933 202 20 HaeII r_GCGC'y Cuts at: 0 295 385 423 573 748 831 941 982 Size: 295 90 38 150 175 83 110 41 Cuts at: 982 1155 Size: 173 Fragments arranged by size: 295 175 173 150 110 90 83 41 38 KasI G'GCGC_C Cuts at: 0 569 1155 Size: 569 586 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1092 1155 Size: 1092 63 MscI TGG'CCA Cuts at: 0 307 416 454 775 1155 Size: 307 109 38 321 380 Fragments arranged by size: 380 321 307 109 38 MslI CAynn'nnrTG Cuts at: 0 410 479 1155 Size: 410 69 676 Fragments arranged by size: 676 410 69 MspA1I CmG'CkG Cuts at: 0 140 393 543 642 821 998 1155 Size: 140 253 150 99 179 177 157 Fragments arranged by size: 253 179 177 157 150 140 99 NaeI GCC'GGC Cuts at: 0 278 568 826 1155 Size: 278 290 258 329 Fragments arranged by size: 329 290 278 258 NarI GG'CG_CC Cuts at: 0 570 1155 Size: 570 585 NgoMIV G'CCGG_C Cuts at: 0 276 566 824 1155 Size: 276 290 258 331 Fragments arranged by size: 331 290 276 258 NotI GC'GGCC_GC Cuts at: 0 611 1155 Size: 611 544 NruI TCG'CGA Cuts at: 0 355 1155 Size: 355 800 NspI r_CATG'y Cuts at: 0 480 1155 Size: 480 675 PciI A'CATG_T Cuts at: 0 476 1155 Size: 476 679 PflMI CCAn_nnn'nTGG Cuts at: 0 941 1155 Size: 941 214 PfoI T'CCnGG_A Cuts at: 0 188 1155 Size: 188 967 PspOMI G'GGCC_C Cuts at: 0 548 1155 Size: 548 607 PstI C_TGCA'G Cuts at: 0 641 1155 Size: 641 514 PvuI CG_AT'CG Cuts at: 0 672 1155 Size: 672 483 PvuII CAG'CTG Cuts at: 0 998 1155 Size: 998 157 SacI G_AGCT'C Cuts at: 0 188 1155 Size: 188 967 SacII CC_GC'GG Cuts at: 0 141 394 1155 Size: 141 253 761 Fragments arranged by size: 761 253 141 SapI GCTCTTCn'nnn_ Cuts at: 0 1069 1155 Size: 1069 86 SfcI C'TryA_G Cuts at: 0 637 1155 Size: 637 518 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1099 1155 Size: 1099 56 SfoI GGC'GCC Cuts at: 0 571 1155 Size: 571 584 StuI AGG'CCT Cuts at: 0 712 1102 1155 Size: 712 390 53 Fragments arranged by size: 712 390 53 StyI C'CwwG_G Cuts at: 0 579 1097 1155 Size: 579 518 58 Fragments arranged by size: 579 518 58 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 73 210 288 1150 1155 Size: 73 137 78 862 5 Fragments arranged by size: 862 137 78 73 5 Enzymes that do cut: AarI AccI AfeI AflIII AleI AlfI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI BbvCI BcgI BglI BlpI Bme1580I BpmI Bpu10I BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstXI BstYI BtgI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI HaeII KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NotI NruI NspI PciI PflMI PfoI PspOMI PstI PvuI PvuII SacI SacII SapI SfcI SfiI SfoI StuI StyI TaqII Enzymes that do not cut: AatII Acc65I AclI AcuI AflII AgeI AhdI AloI ApoI AscI AseI AsiSI AvrII BaeI BbsI BciVI BclI BfrBI BglII BmgBI BmrI BmtI BplI BpuEI BsaI BsaBI BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI NcoI NdeI NheI NsiI PacI PasI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI RsrII SalI SanDI SbfI ScaI SexAI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI