(Circular) (Six-base) MAPSORT of: pFN21AE5364.seq Check: 1156 from: 1 to: 5602 pFN21AE5364 5602 bp With 182 enzymes: SgfI * January 30, 2014 17:18 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3412 278 Size: 53 83 186 936 1876 2468 Fragments arranged by size: 2468 1876 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2945 1937 Size: 1008 4594 Acc65I G'GTAC_C Cuts at: 2923 2923 Size: 5602 AclI AA'CG_TT Cuts at: 1563 3729 4102 1563 Size: 2166 373 3063 Fragments arranged by size: 3063 2166 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2230 3665 5049 5317 1632 Size: 598 1435 1384 268 1917 Fragments arranged by size: 1917 1435 1384 598 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5405 AflIII A'CryG_T Cuts at: 1203 2550 4501 5478 1203 Size: 1347 1951 977 1327 Fragments arranged by size: 1951 1347 1327 977 AgeI A'CCGG_T Cuts at: 4433 4433 Size: 5602 AhdI GACnn_n'nnGTC Cuts at: 4331 4331 Size: 5602 AleI CACnn'nnGTG Cuts at: 1166 2805 1166 Size: 1639 3963 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5602 AlwNI CAG_nnn'CTG Cuts at: 3345 4917 3345 Size: 1572 4030 ApaLI G'TGCA_C Cuts at: 3659 4815 3659 Size: 1156 4446 ApoI r'AATT_y Cuts at: 1493 2911 3137 3289 5398 1493 Size: 1418 226 152 2109 1697 Fragments arranged by size: 2109 1697 1418 226 152 AseI AT'TA_AT Cuts at: 160 1032 4156 160 Size: 872 3124 1606 Fragments arranged by size: 3124 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5602 AvaI C'yCGr_G Cuts at: 1870 1943 2927 1870 Size: 73 984 4545 Fragments arranged by size: 4545 984 73 BamHI G'GATC_C Cuts at: 2932 3299 2932 Size: 367 5235 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2059 2148 2923 4378 618 Size: 325 217 627 272 89 775 1455 1842 Fragments arranged by size: 1842 1455 775 627 325 272 217 89 BanII G_rGCy'C Cuts at: 1391 2824 2873 2921 1391 Size: 1433 49 48 4072 Fragments arranged by size: 4072 1433 49 48 BbeI G_GCGC'C Cuts at: 2152 2152 Size: 5602 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2068 928 Size: 971 169 4462 Fragments arranged by size: 4462 971 169 BbvCI CC'TCA_GC Cuts at: 2275 2275 Size: 5602 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3793 3827 1903 Size: 34 1856 34 3678 Fragments arranged by size: 3678 1856 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3494 4704 3494 Size: 1210 4392 BclI T'GATC_A Cuts at: 1548 1548 Size: 5602 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2894 4213 136 Size: 107 122 71 631 605 1222 1319 1525 Fragments arranged by size: 1525 1319 1222 631 605 122 107 71 BglII A'GATC_T Cuts at: 5597 5597 Size: 5602 BlpI GC'TnA_GC Cuts at: 2024 2045 2823 3019 2024 Size: 21 778 196 4607 Fragments arranged by size: 4607 778 196 21 Bme1580I G_kGCm'C Cuts at: 1790 3663 4819 1790 Size: 1873 1156 2573 Fragments arranged by size: 2573 1873 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5602 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2466 2615 3333 Size: 416 53 83 399 24 1049 149 718 Cuts at: 3333 4291 442 Size: 958 1753 Fragments arranged by size: 1753 1049 958 718 416 399 149 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5602 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2032 2606 2032 Size: 574 5028 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2242 2585 2744 2857 4262 1239 Size: 1003 343 159 113 1405 2579 Fragments arranged by size: 2579 1405 1003 343 159 113 Bpu10I CC'TnA_GC Cuts at: 2275 2275 Size: 5602 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2099 3727 4592 4890 2099 Size: 1628 865 298 2811 Fragments arranged by size: 2811 1628 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2525 4265 882 Size: 617 1026 1740 2219 Fragments arranged by size: 2219 1740 1026 617 BsaAI yAC'GTr Cuts at: 493 1204 3393 493 Size: 711 2189 2702 Fragments arranged by size: 2702 2189 711 BsaBI GATnn'nnATC Cuts at: 5596 5596 Size: 5602 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2149 3409 3791 4426 Size: 53 83 186 936 616 1260 382 635 Cuts at: 4426 275 Size: 1451 Fragments arranged by size: 1451 1260 936 635 616 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4035 4433 4707 4854 5265 4035 Size: 398 274 147 411 4372 Fragments arranged by size: 4372 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5377 Fragments arranged by size: 5377 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2870 1186 Size: 1684 3918 BseYI C'CCAG_C Cuts at: 4805 5301 4805 Size: 496 5106 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2531 1163 Size: 573 795 4234 Fragments arranged by size: 4234 795 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3052 3813 4841 1540 Size: 275 186 1051 761 1028 2301 Fragments arranged by size: 2301 1051 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2824 3663 3748 4819 2824 Size: 839 85 1071 3607 Fragments arranged by size: 3607 1071 839 85 BsmI GAATG_Cn' Cuts at: 2827 3113 3206 2827 Size: 286 93 5223 Fragments arranged by size: 5223 286 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2104 2104 Size: 5602 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2824 2873 2921 3663 3748 4819 1391 Size: 399 1034 49 48 742 85 1071 2174 Fragments arranged by size: 2174 1071 1034 742 399 85 49 48 BspEI T'CCGG_A Cuts at: 5265 5265 Size: 5602 BspHI T'CATG_A Cuts at: 2445 3491 5221 2445 Size: 1046 1730 2826 Fragments arranged by size: 2826 1730 1046 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2957 844 Size: 2113 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2065 3489 1394 Size: 429 242 1424 3507 Fragments arranged by size: 3507 1424 429 242 BsrDI GCAATG_nn' Cuts at: 65 1248 2185 4097 4271 65 Size: 1183 937 1912 174 1396 Fragments arranged by size: 1912 1396 1183 937 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4246 4433 1700 Size: 110 2436 187 2869 Fragments arranged by size: 2869 2436 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5602 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5602 BssSI C'ACGA_G Cuts at: 3662 4674 3662 Size: 1012 4590 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5602 BstBI TT'CG_AA Cuts at: 4406 4406 Size: 5602 BstEII G'GTnAC_C Cuts at: 1184 2768 1184 Size: 1584 4018 BstXI CCAn_nnnn'nTGG Cuts at: 2807 2807 Size: 5602 BstYI r'GATC_y Cuts at: 1972 2541 2932 3299 3684 3701 5153 5239 5251 Size: 569 391 367 385 17 1452 86 12 Cuts at: 5251 5597 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 569 391 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2431 2788 513 Size: 552 939 427 357 3327 Fragments arranged by size: 3327 939 552 427 357 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2143 487 Size: 683 56 917 3946 Fragments arranged by size: 3946 917 683 56 BtsI GCAGTG_nn' Cuts at: 3118 3912 3932 3118 Size: 794 20 4788 Fragments arranged by size: 4788 794 20 ClaI AT'CG_AT Cuts at: 1554 2238 1554 Size: 684 4918 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2773 2808 529 Size: 35 2209 35 3323 Fragments arranged by size: 3323 2209 35 35 DraI TTT'AAA Cuts at: 2906 2906 Size: 5602 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4609 809 Size: 507 3293 1802 Fragments arranged by size: 3293 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2361 2832 3049 Size: 54 1425 50 275 18 531 471 217 Cuts at: 3049 3938 5567 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 531 471 275 217 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3049 1537 Size: 275 1237 4090 Fragments arranged by size: 4090 1237 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2072 2321 2597 3532 1353 Size: 719 249 276 935 3423 Fragments arranged by size: 3423 935 719 276 249 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2912 4189 4563 4709 387 Size: 1064 138 1323 1277 374 146 1280 Fragments arranged by size: 1323 1280 1277 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2822 2822 Size: 5602 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2230 2242 2585 2744 2857 3665 4262 Size: 393 598 12 343 159 113 808 597 Cuts at: 4262 5049 5317 1239 Size: 787 268 1524 Fragments arranged by size: 1524 808 787 598 597 393 343 268 159 113 12 EcoO109I rG'GnC_Cy Cuts at: 2796 2796 Size: 5602 EcoRI G'AATT_C Cuts at: 2911 3289 2911 Size: 378 5224 FspI TGC'GCA Cuts at: 4108 4108 Size: 5602 HaeII r_GCGC'y Cuts at: 1678 2024 2045 2152 4749 1678 Size: 346 21 107 2597 2531 Fragments arranged by size: 2597 2531 346 107 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4243 4275 4317 4349 1373 Size: 32 135 32 2671 32 42 32 2626 Fragments arranged by size: 2671 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2946 3192 669 Size: 988 281 1008 246 3079 Fragments arranged by size: 3079 1008 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5602 HpaI GTT'AAC Cuts at: 3192 3192 Size: 5602 KasI G'GCGC_C Cuts at: 2148 2148 Size: 5602 KpnI G_GTAC'C Cuts at: 2927 2927 Size: 5602 MfeI C'AATT_G Cuts at: 3201 3201 Size: 5602 MluI A'CGCG_T Cuts at: 5478 5478 Size: 5602 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2517 4716 4900 1305 Size: 1212 2199 184 2007 Fragments arranged by size: 2199 2007 1212 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2363 10 Size: 54 1425 343 531 3249 Fragments arranged by size: 3249 1425 531 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2357 2805 3560 3919 4078 518 Size: 648 1191 448 755 359 159 2042 Fragments arranged by size: 2042 1191 755 648 448 359 159 MspA1I CmG'CkG Cuts at: 1606 2279 2506 2613 2805 3018 3695 4843 5088 Size: 673 227 107 192 213 677 1148 245 Cuts at: 5088 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 673 245 227 213 192 107 NarI GG'CG_CC Cuts at: 2149 2149 Size: 5602 NcoI C'CATG_G Cuts at: 513 1065 2004 2788 513 Size: 552 939 784 3327 Fragments arranged by size: 3327 939 784 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5602 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5602 NotI GC'GGCC_GC Cuts at: 3049 3049 Size: 5602 NspI r_CATG'y Cuts at: 1132 1229 2554 2960 4505 1132 Size: 97 1325 406 1545 2229 Fragments arranged by size: 2229 1545 1325 406 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5602 PciI A'CATG_T Cuts at: 2550 4501 2550 Size: 1951 3651 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5602 PfoI T'CCnGG_A Cuts at: 2873 2873 Size: 5602 PmeI GTTT'AAAC Cuts at: 2906 2906 Size: 5602 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2028 2060 3671 3703 5184 5216 2028 Size: 32 1611 32 1481 32 2414 Fragments arranged by size: 2414 1611 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2796 2796 Size: 5602 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5602 PsiI TTA'TAA Cuts at: 3172 3172 Size: 5602 PstI C_TGCA'G Cuts at: 830 2954 830 Size: 2124 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5602 PvuII CAG'CTG Cuts at: 2279 2506 2279 Size: 227 5375 SacI G_AGCT'C Cuts at: 2824 2824 Size: 5602 SalI G'TCGA_C Cuts at: 1936 2944 1936 Size: 1008 4594 SanDI GG'GwC_CC Cuts at: 2796 2796 Size: 5602 SapI GCTCTTCn'nnn_ Cuts at: 2072 2072 Size: 5602 SbfI CC_TGCA'GG Cuts at: 2954 2954 Size: 5602 ScaI AGT'ACT Cuts at: 3850 5562 3850 Size: 1712 3890 SfcI C'TryA_G Cuts at: 826 1045 2950 4085 4766 4957 826 Size: 219 1905 1135 681 191 1471 Fragments arranged by size: 1905 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2150 2150 Size: 5602 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5602 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5602 SmaI CCC'GGG Cuts at: 1872 2929 1872 Size: 1057 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2114 3706 4607 4869 820 Size: 197 926 171 1592 901 262 1553 Fragments arranged by size: 1592 1553 926 901 262 197 171 SnaBI TAC'GTA Cuts at: 493 3393 493 Size: 2900 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5602 SphI G_CATG'C Cuts at: 1132 2960 1132 Size: 1828 3774 SspI AAT'ATT Cuts at: 5 52 3526 5 Size: 47 3474 2081 Fragments arranged by size: 3474 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5602 StyI C'CwwG_G Cuts at: 513 1065 2004 2221 2565 2788 2850 3041 513 Size: 552 939 217 344 223 62 191 3074 Fragments arranged by size: 3074 939 552 344 223 217 191 62 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2448 2708 3629 3646 3799 3984 4429 Size: 1255 868 260 921 17 153 185 445 Cuts at: 4429 325 Size: 1498 Fragments arranged by size: 1498 1255 921 868 445 260 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3848 5560 96 Size: 274 80 33 51 1443 1871 1712 138 Fragments arranged by size: 1871 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5562 5594 5562 Size: 32 5570 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5602 XbaI T'CTAG_A Cuts at: 2938 2938 Size: 5602 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2266 2371 2786 2807 2266 Size: 105 415 21 5061 Fragments arranged by size: 5061 415 105 21 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5602 XmaI C'CCGG_G Cuts at: 1870 2927 1870 Size: 1057 4545 XmnI GAAnn'nnTTC Cuts at: 3731 3731 Size: 5602 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3410 276 Size: 53 83 186 936 1876 2468 Fragments arranged by size: 2468 1876 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SrfI SwaI