(Circular) (Six-base) MAPSORT of: pFN21AE5426.seq Check: 8356 from: 1 to: 5650 pFN21AE5426 5650 bp With 182 enzymes: SgfI * January 30, 2014 17:19 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3460 278 Size: 53 83 186 936 1924 2468 Fragments arranged by size: 2468 1924 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2993 1937 Size: 1056 4594 Acc65I G'GTAC_C Cuts at: 2971 2971 Size: 5650 AclI AA'CG_TT Cuts at: 1563 3777 4150 1563 Size: 2214 373 3063 Fragments arranged by size: 3063 2214 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2302 2721 2860 2918 3713 5097 5365 1632 Size: 670 419 139 58 795 1384 268 1917 Fragments arranged by size: 1917 1384 795 670 419 268 139 58 AfeI AGC'GCT Cuts at: 2273 2273 Size: 5650 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5453 AflIII A'CryG_T Cuts at: 1203 4549 5526 1203 Size: 3346 977 1327 Fragments arranged by size: 3346 1327 977 AgeI A'CCGG_T Cuts at: 4481 4481 Size: 5650 AhdI GACnn_n'nnGTC Cuts at: 2260 4379 2260 Size: 2119 3531 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5650 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5650 AlwNI CAG_nnn'CTG Cuts at: 3393 4965 3393 Size: 1572 4078 ApaLI G'TGCA_C Cuts at: 2756 3707 4863 2756 Size: 951 1156 3543 Fragments arranged by size: 3543 1156 951 ApoI r'AATT_y Cuts at: 1493 2036 2959 3185 3337 5446 1493 Size: 543 923 226 152 2109 1697 Fragments arranged by size: 2109 1697 923 543 226 152 AseI AT'TA_AT Cuts at: 160 1032 4204 160 Size: 872 3172 1606 Fragments arranged by size: 3172 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5650 AvaI C'yCGr_G Cuts at: 1870 1943 2975 1870 Size: 73 1032 4545 Fragments arranged by size: 4545 1032 73 BamHI G'GATC_C Cuts at: 2513 2980 3347 2513 Size: 467 367 4816 Fragments arranged by size: 4816 467 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2945 2971 4426 618 Size: 325 217 627 1158 26 1455 1842 Fragments arranged by size: 1842 1455 1158 627 325 217 26 BanII G_rGCy'C Cuts at: 1391 2417 2969 1391 Size: 1026 552 4072 Fragments arranged by size: 4072 1026 552 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4679 BbvCI CC'TCA_GC Cuts at: 2861 2924 2861 Size: 63 5587 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3841 3875 1903 Size: 34 50 34 1820 34 3678 Fragments arranged by size: 3678 1820 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3542 4752 3542 Size: 1210 4440 BclI T'GATC_A Cuts at: 1548 1548 Size: 5650 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2288 2421 2439 Size: 107 122 71 631 605 616 133 18 Cuts at: 2439 4261 136 Size: 1822 1525 Fragments arranged by size: 1822 1525 631 616 605 133 122 107 71 18 BglII A'GATC_T Cuts at: 5645 5645 Size: 5650 BlpI GC'TnA_GC Cuts at: 3067 3067 Size: 5650 Bme1580I G_kGCm'C Cuts at: 1790 2760 3711 4867 1790 Size: 970 951 1156 2573 Fragments arranged by size: 2573 1156 970 951 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5650 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3381 4339 442 Size: 416 53 83 399 24 1964 958 1753 Fragments arranged by size: 1964 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5650 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2173 4310 1239 Size: 934 2137 2579 Fragments arranged by size: 2579 2137 934 Bpu10I CC'TnA_GC Cuts at: 2861 2924 2861 Size: 63 5587 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2771 3775 4640 4938 2771 Size: 1004 865 298 3483 Fragments arranged by size: 3483 1004 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4313 882 Size: 617 2814 2219 Fragments arranged by size: 2814 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2374 3441 493 Size: 711 1170 1067 2702 Fragments arranged by size: 2702 1170 1067 711 BsaBI GATnn'nnATC Cuts at: 5644 5644 Size: 5650 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3457 3839 4474 275 Size: 53 83 186 936 1924 382 635 1451 Fragments arranged by size: 1924 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4083 4481 4755 4902 5313 4083 Size: 398 274 147 411 4420 Fragments arranged by size: 4420 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2009 2039 1178 Size: 30 165 30 606 30 4789 Fragments arranged by size: 4789 606 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2633 2642 1186 Size: 1447 9 4194 Fragments arranged by size: 4194 1447 9 BseYI C'CCAG_C Cuts at: 2008 2122 2268 2629 4853 5349 2008 Size: 114 146 361 2224 496 2309 Fragments arranged by size: 2309 2224 496 361 146 114 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5077 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2368 3100 3861 4889 1540 Size: 275 186 367 732 761 1028 2301 Fragments arranged by size: 2301 1028 761 732 367 275 186 BsiHKAI G_wGCw'C Cuts at: 2409 2760 3711 3796 4867 2409 Size: 351 951 85 1071 3192 Fragments arranged by size: 3192 1071 951 351 85 BsmI GAATG_Cn' Cuts at: 3161 3254 3161 Size: 93 5557 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2409 2417 2760 2969 3711 3796 4867 Size: 399 619 8 343 209 742 85 1071 Cuts at: 4867 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 619 399 343 209 85 8 BspEI T'CCGG_A Cuts at: 5313 5313 Size: 5650 BspHI T'CATG_A Cuts at: 2409 3539 5269 2409 Size: 1130 1730 2790 Fragments arranged by size: 2790 1730 1130 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2139 2265 3005 844 Size: 1295 126 740 3489 Fragments arranged by size: 3489 1295 740 126 BsrBI CCG'CTC Cuts at: 1394 1823 3537 1394 Size: 429 1714 3507 Fragments arranged by size: 3507 1714 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4145 4319 65 Size: 1183 2897 174 1396 Fragments arranged by size: 2897 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4294 4481 1700 Size: 110 2484 187 2869 Fragments arranged by size: 2869 2484 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5650 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5650 BssSI C'ACGA_G Cuts at: 3710 4722 3710 Size: 1012 4638 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5650 BstBI TT'CG_AA Cuts at: 4454 4454 Size: 5650 BstEII G'GTnAC_C Cuts at: 1184 2479 1184 Size: 1295 4355 BstYI r'GATC_y Cuts at: 1972 2214 2513 2523 2688 2980 3347 3732 3749 Size: 242 299 10 165 292 367 385 17 Cuts at: 3749 5201 5287 5299 5645 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 299 292 242 165 86 17 12 10 Bsu36I CC'TnA_GG Cuts at: 2882 2941 2882 Size: 59 5591 BtgI C'CryG_G Cuts at: 513 1065 2226 2417 513 Size: 552 1161 191 3746 Fragments arranged by size: 3746 1161 552 191 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2263 487 Size: 683 56 1037 3874 Fragments arranged by size: 3874 1037 683 56 BtsI GCAGTG_nn' Cuts at: 2117 2624 3166 3960 3980 2117 Size: 507 542 794 20 3787 Fragments arranged by size: 3787 794 542 507 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5650 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2538 2573 529 Size: 35 1974 35 3606 Fragments arranged by size: 3606 1974 35 35 DraI TTT'AAA Cuts at: 2954 2954 Size: 5650 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4657 809 Size: 507 3341 1802 Fragments arranged by size: 3341 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2142 2229 2250 Size: 54 1425 50 275 18 312 87 21 Cuts at: 2250 2376 2487 2673 2829 3097 3986 5615 8 Size: 126 111 186 156 268 889 1629 43 Fragments arranged by size: 1629 1425 889 312 275 268 186 156 126 111 87 54 50 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 3097 1537 Size: 275 1285 4090 Fragments arranged by size: 4090 1285 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2090 2192 2906 3580 1353 Size: 737 102 714 674 3423 Fragments arranged by size: 3423 737 714 674 102 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4237 4611 4757 387 Size: 1064 138 2648 374 146 1280 Fragments arranged by size: 2648 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2173 2302 2721 2860 2918 3713 4310 Size: 393 541 129 419 139 58 795 597 Cuts at: 4310 5097 5365 1239 Size: 787 268 1524 Fragments arranged by size: 1524 795 787 597 541 419 393 268 139 129 58 EcoO109I rG'GnC_Cy Cuts at: 2798 2916 2798 Size: 118 5532 EcoRI G'AATT_C Cuts at: 2036 2959 3337 2036 Size: 923 378 4349 Fragments arranged by size: 4349 923 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2935 2935 Size: 5650 FspI TGC'GCA Cuts at: 4156 4156 Size: 5650 HaeII r_GCGC'y Cuts at: 1678 2275 4797 1678 Size: 597 2522 2531 Fragments arranged by size: 2531 2522 597 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2246 2278 4291 4323 4365 Size: 32 135 32 674 32 2013 32 42 Cuts at: 4365 4397 1373 Size: 32 2626 Fragments arranged by size: 2626 2013 674 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2994 3240 669 Size: 988 281 1056 246 3079 Fragments arranged by size: 3079 1056 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5650 HpaI GTT'AAC Cuts at: 3240 3240 Size: 5650 KpnI G_GTAC'C Cuts at: 2975 2975 Size: 5650 MfeI C'AATT_G Cuts at: 3249 3249 Size: 5650 MluI A'CGCG_T Cuts at: 5526 5526 Size: 5650 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2098 2521 2687 4764 4948 1305 Size: 793 423 166 2077 184 2007 Fragments arranged by size: 2077 2007 793 423 184 166 MscI TGG'CCA Cuts at: 10 64 1489 1832 2144 2231 2378 2831 10 Size: 54 1425 343 312 87 147 453 2829 Fragments arranged by size: 2829 1425 453 343 312 147 87 54 MslI CAynn'nnrTG Cuts at: 518 1166 2027 2177 2354 2471 2825 3608 3967 Size: 648 861 150 177 117 354 783 359 Cuts at: 3967 4126 518 Size: 159 2042 Fragments arranged by size: 2042 861 783 648 359 354 177 159 150 117 MspA1I CmG'CkG Cuts at: 1606 2588 3066 3743 4891 5136 1606 Size: 982 478 677 1148 245 2120 Fragments arranged by size: 2120 1148 982 677 478 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5098 NdeI CA'TA_TG Cuts at: 387 387 Size: 5650 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5650 NotI GC'GGCC_GC Cuts at: 3097 3097 Size: 5650 NspI r_CATG'y Cuts at: 1132 1229 2248 3008 4553 1132 Size: 97 1019 760 1545 2229 Fragments arranged by size: 2229 1545 1019 760 97 PasI CC'CwG_GG Cuts at: 1348 2596 1348 Size: 1248 4402 PciI A'CATG_T Cuts at: 4549 4549 Size: 5650 PflMI CCAn_nnn'nTGG Cuts at: 1108 2151 1108 Size: 1043 4607 PmeI GTTT'AAAC Cuts at: 2954 2954 Size: 5650 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3719 3751 5232 5264 3719 Size: 32 1481 32 4105 Fragments arranged by size: 4105 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2798 2916 2798 Size: 118 5532 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5650 PsiI TTA'TAA Cuts at: 3220 3220 Size: 5650 PstI C_TGCA'G Cuts at: 830 3002 830 Size: 2172 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5650 PvuII CAG'CTG Cuts at: 2588 2588 Size: 5650 SalI G'TCGA_C Cuts at: 1936 2992 1936 Size: 1056 4594 SapI GCTCTTCn'nnn_ Cuts at: 2090 2090 Size: 5650 SbfI CC_TGCA'GG Cuts at: 3002 3002 Size: 5650 ScaI AGT'ACT Cuts at: 3898 5610 3898 Size: 1712 3938 SfcI C'TryA_G Cuts at: 826 1045 2998 4133 4814 5005 826 Size: 219 1953 1135 681 191 1471 Fragments arranged by size: 1953 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5650 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5650 SmaI CCC'GGG Cuts at: 1872 2977 1872 Size: 1105 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2786 3754 4655 4917 820 Size: 197 926 843 968 901 262 1553 Fragments arranged by size: 1553 968 926 901 843 262 197 SnaBI TAC'GTA Cuts at: 493 3441 493 Size: 2948 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5650 SphI G_CATG'C Cuts at: 1132 2248 3008 1132 Size: 1116 760 3774 Fragments arranged by size: 3774 1116 760 SspI AAT'ATT Cuts at: 5 52 3574 5 Size: 47 3522 2081 Fragments arranged by size: 3522 2081 47 StuI AGG'CCT Cuts at: 1517 2717 1517 Size: 1200 4450 StyI C'CwwG_G Cuts at: 513 1065 3089 513 Size: 552 2024 3074 Fragments arranged by size: 3074 2024 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2282 2348 2452 3677 3694 3847 4032 Size: 1255 702 66 104 1225 17 153 185 Cuts at: 4032 4477 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1225 702 445 185 153 104 66 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3896 5608 96 Size: 274 80 33 51 1443 1919 1712 138 Fragments arranged by size: 1919 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5610 5642 5610 Size: 32 5618 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5650 XbaI T'CTAG_A Cuts at: 2986 2986 Size: 5650 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2284 2284 Size: 5650 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5650 XmaI C'CCGG_G Cuts at: 1870 2975 1870 Size: 1105 4545 XmnI GAAnn'nnTTC Cuts at: 3779 3779 Size: 5650 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3458 276 Size: 53 83 186 936 1924 2468 Fragments arranged by size: 2468 1924 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI