(Circular) (Six-base) MAPSORT of: pFN21AE5331.seq Check: 2040 from: 1 to: 5632 pFN21AE5331 5632 bp With 182 enzymes: SgfI * January 30, 2014 17:17 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3442 278 Size: 53 83 186 936 1906 2468 Fragments arranged by size: 2468 1906 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2975 1937 Size: 1038 4594 Acc65I G'GTAC_C Cuts at: 2953 2953 Size: 5632 AclI AA'CG_TT Cuts at: 1563 3759 4132 1563 Size: 2196 373 3063 Fragments arranged by size: 3063 2196 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2902 3695 5079 5347 1632 Size: 1270 793 1384 268 1917 Fragments arranged by size: 1917 1384 1270 793 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5435 AflIII A'CryG_T Cuts at: 1203 4531 5508 1203 Size: 3328 977 1327 Fragments arranged by size: 3328 1327 977 AgeI A'CCGG_T Cuts at: 4463 4463 Size: 5632 AhdI GACnn_n'nnGTC Cuts at: 4361 4361 Size: 5632 AleI CACnn'nnGTG Cuts at: 1166 2814 1166 Size: 1648 3984 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5632 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2421 2453 2421 Size: 32 5600 AlwNI CAG_nnn'CTG Cuts at: 3375 4947 3375 Size: 1572 4060 ApaLI G'TGCA_C Cuts at: 3689 4845 3689 Size: 1156 4476 ApoI r'AATT_y Cuts at: 1493 2036 2467 2941 3167 3319 5428 1493 Size: 543 431 474 226 152 2109 1697 Fragments arranged by size: 2109 1697 543 474 431 226 152 AseI AT'TA_AT Cuts at: 160 1032 4186 160 Size: 872 3154 1606 Fragments arranged by size: 3154 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5632 AvaI C'yCGr_G Cuts at: 1870 1943 2123 2957 1870 Size: 73 180 834 4545 Fragments arranged by size: 4545 834 180 73 AvrII C'CTAG_G Cuts at: 2770 2770 Size: 5632 BamHI G'GATC_C Cuts at: 2643 2962 3329 2643 Size: 319 367 4946 Fragments arranged by size: 4946 367 319 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2953 4408 618 Size: 325 217 627 1166 1455 1842 Fragments arranged by size: 1842 1455 1166 627 325 217 BanII G_rGCy'C Cuts at: 1391 2905 2951 1391 Size: 1514 46 4072 Fragments arranged by size: 4072 1514 46 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4661 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3823 3857 1903 Size: 34 1886 34 3678 Fragments arranged by size: 3678 1886 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3524 4734 3524 Size: 1210 4422 BclI T'GATC_A Cuts at: 1548 1548 Size: 5632 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4243 136 Size: 107 122 71 631 605 2571 1525 Fragments arranged by size: 2571 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5627 5627 Size: 5632 BlpI GC'TnA_GC Cuts at: 3049 3049 Size: 5632 Bme1580I G_kGCm'C Cuts at: 1790 3693 4849 1790 Size: 1903 1156 2573 Fragments arranged by size: 2573 1903 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5632 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3363 4321 442 Size: 416 53 83 399 24 1946 958 1753 Fragments arranged by size: 1946 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5632 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4292 1239 Size: 3053 2579 Bpu10I CC'TnA_GC Cuts at: 2697 2697 Size: 5632 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3757 4622 4920 3757 Size: 865 298 4469 Fragments arranged by size: 4469 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4295 882 Size: 617 2796 2219 Fragments arranged by size: 2796 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2681 3423 493 Size: 711 1477 742 2702 Fragments arranged by size: 2702 1477 742 711 BsaBI GATnn'nnATC Cuts at: 5626 5626 Size: 5632 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3439 3821 4456 275 Size: 53 83 186 936 1906 382 635 1451 Fragments arranged by size: 1906 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4065 4463 4737 4884 5295 4065 Size: 398 274 147 411 4402 Fragments arranged by size: 4402 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5407 Fragments arranged by size: 5407 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5632 BseYI C'CCAG_C Cuts at: 4835 5331 4835 Size: 496 5136 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2378 2494 1163 Size: 573 642 116 4301 Fragments arranged by size: 4301 642 573 116 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3082 3843 4871 1540 Size: 275 186 1081 761 1028 2301 Fragments arranged by size: 2301 1081 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2124 3693 3778 4849 2124 Size: 1569 85 1071 2907 Fragments arranged by size: 2907 1569 1071 85 BsmI GAATG_Cn' Cuts at: 3143 3236 3143 Size: 93 5539 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2124 2905 2951 3693 3778 4849 1391 Size: 399 334 781 46 742 85 1071 2174 Fragments arranged by size: 2174 1071 781 742 399 334 85 46 BspEI T'CCGG_A Cuts at: 5295 5295 Size: 5632 BspHI T'CATG_A Cuts at: 2409 3521 5251 2409 Size: 1112 1730 2790 Fragments arranged by size: 2790 1730 1112 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2987 844 Size: 2143 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3519 1394 Size: 429 1696 3507 Fragments arranged by size: 3507 1696 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4127 4301 65 Size: 1183 2879 174 1396 Fragments arranged by size: 2879 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4276 4463 1700 Size: 110 2466 187 2869 Fragments arranged by size: 2869 2466 187 110 BsrGI T'GTAC_A Cuts at: 96 2835 96 Size: 2739 2893 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5632 BssSI C'ACGA_G Cuts at: 3692 4704 3692 Size: 1012 4620 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5632 BstBI TT'CG_AA Cuts at: 4436 4436 Size: 5632 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5632 BstXI CCAn_nnnn'nTGG Cuts at: 2110 2110 Size: 5632 BstYI r'GATC_y Cuts at: 1972 2643 2962 3329 3714 3731 5183 5269 5281 Size: 671 319 367 385 17 1452 86 12 Cuts at: 5281 5627 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 671 385 367 346 319 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4141 Fragments arranged by size: 4141 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4893 Fragments arranged by size: 4893 683 56 BtsI GCAGTG_nn' Cuts at: 3148 3942 3962 3148 Size: 794 20 4818 Fragments arranged by size: 4818 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5632 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5597 DraI TTT'AAA Cuts at: 2936 2936 Size: 5632 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4639 809 Size: 507 3323 1802 Fragments arranged by size: 3323 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2145 2376 2844 Size: 54 1425 50 275 18 315 231 468 Cuts at: 2844 3079 3968 5597 8 Size: 235 889 1629 43 Fragments arranged by size: 1629 1425 889 468 315 275 235 231 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3079 1537 Size: 275 1267 4090 Fragments arranged by size: 4090 1267 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3562 1353 Size: 2209 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4219 4593 4739 387 Size: 1064 138 2630 374 146 1280 Fragments arranged by size: 2630 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2902 3695 4292 5079 5347 1239 Size: 393 1270 793 597 787 268 1524 Fragments arranged by size: 1524 1270 793 787 597 393 268 EcoRI G'AATT_C Cuts at: 2467 2941 3319 2467 Size: 474 378 4780 Fragments arranged by size: 4780 474 378 FspI TGC'GCA Cuts at: 4138 4138 Size: 5632 HaeII r_GCGC'y Cuts at: 1678 4779 1678 Size: 3101 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4273 4305 4347 4379 1373 Size: 32 135 32 2701 32 42 32 2626 Fragments arranged by size: 2701 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2976 3222 669 Size: 988 281 1038 246 3079 Fragments arranged by size: 3079 1038 988 281 246 HindIII A'AGCT_T Cuts at: 748 2482 748 Size: 1734 3898 HpaI GTT'AAC Cuts at: 3222 3222 Size: 5632 KpnI G_GTAC'C Cuts at: 2957 2957 Size: 5632 MfeI C'AATT_G Cuts at: 3231 3231 Size: 5632 MluI A'CGCG_T Cuts at: 5508 5508 Size: 5632 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2220 4746 4930 1305 Size: 915 2526 184 2007 Fragments arranged by size: 2526 2007 915 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2147 2378 2846 10 Size: 54 1425 343 315 231 468 2796 Fragments arranged by size: 2796 1425 468 343 315 231 54 MslI CAynn'nnrTG Cuts at: 518 1166 2177 2303 2597 2814 2852 3590 3949 Size: 648 1011 126 294 217 38 738 359 Cuts at: 3949 4108 518 Size: 159 2042 Fragments arranged by size: 2042 1011 738 648 359 294 217 159 126 38 MspA1I CmG'CkG Cuts at: 1606 3048 3725 4873 5118 1606 Size: 1442 677 1148 245 2120 Fragments arranged by size: 2120 1442 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4141 Fragments arranged by size: 4141 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5632 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5632 NotI GC'GGCC_GC Cuts at: 3079 3079 Size: 5632 NspI r_CATG'y Cuts at: 1132 1229 2990 4535 1132 Size: 97 1761 1545 2229 Fragments arranged by size: 2229 1761 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5632 PciI A'CATG_T Cuts at: 4531 4531 Size: 5632 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5632 PfoI T'CCnGG_A Cuts at: 2447 2447 Size: 5632 PmeI GTTT'AAAC Cuts at: 2936 2936 Size: 5632 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3701 3733 5214 5246 3701 Size: 32 1481 32 4087 Fragments arranged by size: 4087 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5632 PsiI TTA'TAA Cuts at: 3202 3202 Size: 5632 PstI C_TGCA'G Cuts at: 830 2984 830 Size: 2154 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5632 SalI G'TCGA_C Cuts at: 1936 2974 1936 Size: 1038 4594 SbfI CC_TGCA'GG Cuts at: 2984 2984 Size: 5632 ScaI AGT'ACT Cuts at: 3880 5592 3880 Size: 1712 3920 SexAI A'CCwGG_T Cuts at: 2109 2109 Size: 5632 SfcI C'TryA_G Cuts at: 826 1045 2872 2980 4115 4796 4987 826 Size: 219 1827 108 1135 681 191 1471 Fragments arranged by size: 1827 1471 1135 681 219 191 108 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5632 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5632 SmaI CCC'GGG Cuts at: 1872 2959 1872 Size: 1087 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3736 4637 4899 820 Size: 197 926 1793 901 262 1553 Fragments arranged by size: 1793 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3423 493 Size: 2930 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5632 SphI G_CATG'C Cuts at: 1132 2990 1132 Size: 1858 3774 SspI AAT'ATT Cuts at: 5 52 3556 5 Size: 47 3504 2081 Fragments arranged by size: 3504 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5632 StyI C'CwwG_G Cuts at: 513 1065 2004 2770 3071 513 Size: 552 939 766 301 3074 Fragments arranged by size: 3074 939 766 552 301 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2315 3659 3676 3829 4014 4459 325 Size: 1255 735 1344 17 153 185 445 1498 Fragments arranged by size: 1498 1344 1255 735 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 2317 2706 Size: 274 80 33 51 1443 204 136 389 Cuts at: 2706 2835 3878 5590 96 Size: 129 1043 1712 138 Fragments arranged by size: 1712 1443 1043 389 274 204 138 136 129 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5592 5624 5592 Size: 32 5600 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5632 XbaI T'CTAG_A Cuts at: 2968 2968 Size: 5632 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5632 XmaI C'CCGG_G Cuts at: 1870 2957 1870 Size: 1087 4545 XmnI GAAnn'nnTTC Cuts at: 2040 3761 2040 Size: 1721 3911 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3440 276 Size: 53 83 186 936 1906 2468 Fragments arranged by size: 2468 1906 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI