Restriction map for
pFN21AE5433
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI Aor51HI ApaLI
AscI Asp718I AspEI AspHI AspI
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmI BspDI BspEI BspLU11I
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstYI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HhaI HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MflI
MluI MroI MunI MvnI MwoI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I Van91I XbaI
XcmI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AciI 363 CCGC CCGC(-3/-1)
AcyI 610 GGCGTC G[GA]^CG[CT]C
AocI 873 CCTGAGG CC^T[ACGT]AGG
ApaI 896 GGGCCC GGGCC^C
AseI 580 ATTAAT AT^TAAT
AsnI 580 ATTAAT AT^TAAT
AvaI 472 CTCGAG C^[CT]CG[GA]G
AvaII 440 GGTCC G^G[AT]CC
BanII 896 GGGCCC G[GA]GC[CT]^C
BbvCI 686 CCTCAGC CCTCAGC(-5/-2)
BbvI 787 GCAGC GCAGC(8/12)
BcnI 119 CCGGG CC^[GC]GG
BmyI 896 GGGCCC G[AGT]GC[ACT]^C
BsaHI 610 GGCGTC G[GA]^CG[CT]C
BsaI 475 GAGACC GGTCTC(1/5)
BsmFI 232 GTCCC GGGAC(10/14)
BsoBI 472 CTCGAG C^[CT]CG[GA]G
Bsp1286I 896 GGGCCC G[AGT]GC[ACT]^C
Bsp1407I 830 TGTACA T^GTACA
BspHI 404 TCATGA T^CATGA
BspMI 125 ACCTGC ACCTGC(4/8)
BsrGI 830 TGTACA T^GTACA
BstXI 414 CCAGCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 873 CCTGAGG CC^T[ACGT]AGG
DpnII 538 GATC ^GATC
DsaI 836 CCATGG C^C[GA][CT]GG
EarI 891 GAAGAG CTCTTC(1/4)
Eco81I 873 CCTGAGG CC^T[ACGT]AGG
EcoT14I 836 CCATGG C^C[AT][AT]GG
Fnu4HI 787 GCAGC GC^[ACGT]GC
HapII 119 CCGG C^CGG
HgaI 611 GCGTC GACGC(5/10)
Hin1I 610 GGCGTC G[GA]^CG[CT]C
HpaII 119 CCGG C^CGG
ItaI 787 GCAGC GC^[ACGT]GC
Ksp632I 891 GAAGAG CTCTTC(1/4)
MboI 538 GATC ^GATC
MspA1I 689 CAGCTG C[AC]G^C[GT]G
MspI 119 CCGG C^CGG
NciI 119 CCGGG CC^[GC]GG
NcoI 836 CCATGG C^CATGG
NdeII 538 GATC ^GATC
NspI 21 ACATGC [GA]CATG^[CT]
PaeR7I 472 CTCGAG C^TCGAG
PpuMI 439 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 580 ATTAAT AT^TAAT
PvuII 689 CAGCTG CAG^CTG
RcaI 404 TCATGA T^CATGA
Sau3AI 538 GATC ^GATC
SmlI 472 CTCGAG C^T[CT][GA]AG
SspBI 830 TGTACA T^GTACA
SspI 639 AATATT AAT^ATT
StyI 836 CCATGG C^C[AT][AT]GG
TaqI 473 TCGA T^CGA
TfiI 66 GATTC G^A[AT]TC
TseI 787 GCAGC G^C[AT]GC
TthHB8I 473 TCGA T^CGA
XhoI 472 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
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Asp700I 264 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
855 GAACCCCTTC
BalI 140 TGGCCA TGG^CCA
371 TGGCCA
BfaI 106 CTAG C^TAG
803 CTAG
BseRI 37 CTCCTC GAGGAG(10/8)
910 CTCCTC
BsgI 388 CTGCAC GTGCAG(16/14)
504 CTGCAC
BsrI 607 ACTGG ACTGG(1/-1)
634 ACTGG
BstEII 741 GGTCACC G^GT[ACGT]ACC
840 GGTCACC
BstF5I 135 CATCC GGATG(2/0)
322 GGATG
BstPI 741 GGTCACC G^GT[ACGT]ACC
840 GGTCACC
Cac8I 283 GCAGGC GC[ACGT]^[ACGT]GC
558 GCTCGC
EaeI 140 TGGCCA [CT]^GGCC[GA]
371 TGGCCA
EcoO65I 741 GGTCACC G^GT[ACGT]ACC
840 GGTCACC
FokI 135 CATCC GGATG(9/13)
322 GGATG
MaeI 106 CTAG C^TAG
803 CTAG
MaeII 58 ACGT A^CGT
587 ACGT
MluNI 140 TGGCCA TGG^CCA
371 TGGCCA
MscI 140 TGGCCA TGG^CCA
371 TGGCCA
MseI 581 TTAA T^TAA
932 TTAA
NdeI 144 CATATG CA^TATG
278 CATATG
PleI 154 GACTC GAGTC(4/5)
908 GACTC
PstI 785 CTGCAG CTGCA^G
947 CTGCAG
SfaNI 323 GATGC GCATC(5/9)
449 GCATC
Tru9I 581 TTAA T^TAA
932 TTAA
XmnI 264 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
855 GAACCCCTTC
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AcsI AfaI AluI ApoI BsaJI
BsiYI BslI BsmAI BssKI BstNI
Cfr13I DdeI DraII EcoO109I EcoRII
HaeIII HinfI HphI MaeIII MboII
MnlI MslI MvaI NlaIII NlaIV
RsaI Sau96I ScrFI SfcI Tsp45I
Tsp509I TspRI