Restriction map for
pFN21AE5433
(Linear) (Six-base) MAPSORT of: KIEE5433.seq Check: 3347 from: 1 to: 960
KIEE5433.orf
With 182 enzymes: SgfI *
January 29, 2014 12:00 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 960
Size: 574 386
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 416 448 960
Size: 416 32 512
Fragments arranged by size:
512 416 32
ApaI G_GGCC'C
Cuts at: 0 900 960
Size: 900 60
ApoI r'AATT_y
Cuts at: 0 31 268 518 960
Size: 31 237 250 442
Fragments arranged by size:
442 250 237 31
AseI AT'TA_AT
Cuts at: 0 581 960
Size: 581 379
AvaI C'yCGr_G
Cuts at: 0 472 960
Size: 472 488
BanII G_rGCy'C
Cuts at: 0 900 960
Size: 900 60
BbvCI CC'TCA_GC
Cuts at: 0 687 960
Size: 687 273
Bme1580I G_kGCm'C
Cuts at: 0 900 960
Size: 900 60
Bpu10I CC'TnA_GC
Cuts at: 0 687 960
Size: 687 273
BsaI GGTCTCn'nnnn_
Cuts at: 0 469 960
Size: 469 491
BsaHI Gr'CG_yC
Cuts at: 0 611 960
Size: 611 349
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 901 960
Size: 28 873 59
Fragments arranged by size:
873 59 28
BseYI C'CCAG_C
Cuts at: 0 413 691 960
Size: 413 278 269
Fragments arranged by size:
413 278 269
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 489 960
Size: 373 116 471
Fragments arranged by size:
471 373 116
Bsp1286I G_dGCh'C
Cuts at: 0 900 960
Size: 900 60
BspHI T'CATG_A
Cuts at: 0 404 960
Size: 404 556
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 134 960
Size: 134 826
BsrGI T'GTAC_A
Cuts at: 0 830 960
Size: 830 130
BstEII G'GTnAC_C
Cuts at: 0 741 840 960
Size: 741 99 120
Fragments arranged by size:
741 120 99
BstXI CCAn_nnnn'nTGG
Cuts at: 0 421 960
Size: 421 539
Bsu36I CC'TnA_GG
Cuts at: 0 874 960
Size: 874 86
BtgI C'CryG_G
Cuts at: 0 836 960
Size: 836 124
BtsI GCAGTG_nn'
Cuts at: 0 944 960
Size: 944 16
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 797 832 960
Size: 797 35 128
Fragments arranged by size:
797 128 35
EaeI y'GGCC_r
Cuts at: 0 140 371 960
Size: 140 231 589
Fragments arranged by size:
589 231 140
EarI CTCTTCn'nnn_
Cuts at: 0 886 960
Size: 886 74
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 960
Size: 574 386
EcoO109I rG'GnC_Cy
Cuts at: 0 440 896 897 960
Size: 440 456 1 63
Fragments arranged by size:
456 440 63 1
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 960
Size: 215 745
MscI TGG'CCA
Cuts at: 0 142 373 960
Size: 142 231 587
Fragments arranged by size:
587 231 142
MslI CAynn'nnrTG
Cuts at: 0 172 321 511 847 886 960
Size: 172 149 190 336 39 74
Fragments arranged by size:
336 190 172 149 74 39
MspA1I CmG'CkG
Cuts at: 0 691 960
Size: 691 269
NcoI C'CATG_G
Cuts at: 0 836 960
Size: 836 124
NdeI CA'TA_TG
Cuts at: 0 145 279 960
Size: 145 134 681
Fragments arranged by size:
681 145 134
NspI r_CATG'y
Cuts at: 0 25 960
Size: 25 935
PasI CC'CwG_GG
Cuts at: 0 900 960
Size: 900 60
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 55 87 960
Size: 55 32 873
Fragments arranged by size:
873 55 32
PpuMI rG'GwC_Cy
Cuts at: 0 440 960
Size: 440 520
PspOMI G'GGCC_C
Cuts at: 0 896 960
Size: 896 64
PstI C_TGCA'G
Cuts at: 0 789 951 960
Size: 789 162 9
Fragments arranged by size:
789 162 9
PvuII CAG'CTG
Cuts at: 0 691 960
Size: 691 269
SfcI C'TryA_G
Cuts at: 0 785 867 947 960
Size: 785 82 80 13
Fragments arranged by size:
785 82 80 13
SmlI C'TyrA_G
Cuts at: 0 472 960
Size: 472 488
SspI AAT'ATT
Cuts at: 0 641 960
Size: 641 319
StyI C'CwwG_G
Cuts at: 0 836 960
Size: 836 124
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 607 960
Size: 607 353
TatI w'GTAC_w
Cuts at: 0 176 830 960
Size: 176 654 130
Fragments arranged by size:
654 176 130
XhoI C'TCGA_G
Cuts at: 0 472 960
Size: 472 488
XmnI GAAnn'nnTTC
Cuts at: 0 268 859 960
Size: 268 591 101
Fragments arranged by size:
591 268 101
Enzymes that do cut:
AcuI AloI ApaI ApoI AseI AvaI BanII BbvCI
Bme1580I Bpu10I BsaI BsaHI BseRI BseYI BsgI Bsp1286I
BspHI BspMI BsrGI BstEII BstXI Bsu36I BtgI BtsI
CspCI EaeI EarI Eco57MI EcoO109I MmeI MscI MslI
MspA1I NcoI NdeI NspI PasI PpiI PpuMI PspOMI
PstI PvuII SfcI SmlI SspI StyI TaqII TatI
XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI AscI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BplI BpmI BpuEI BsaAI BsaBI BsaWI BsaXI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstYI BstZ17I BtgZI ClaI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NgoMIV NheI NotI NruI NsiI PacI PciI PflMI
PfoI PmeI PmlI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI TstI Tth111I XbaI XcmI
XmaI ZraI