################################## # NO CUT ENZYMES ( 166 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AviII AvrII BamHI BanI BbeI BbrPI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiHKAI BsiWI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoO109I EcoO65I EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MflI MluI MroI MseI MslI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI Tru9I TspRI TthHB8I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AcsI 112 GAATTC [GA]^AATT[CT] AfaI 9 GTAC GT^AC ApoI 112 GAATTC [GA]^AATT[CT] AspI 279 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 74 CCCGGG C^[CT]CG[GA]G AvaII 257 GGTCC G^G[AT]CC BalI 2 TGGCCA TGG^CCA BanII 144 GGGCTC G[GA]GC[CT]^C BbsI 220 GAAGAC GAAGAC(2/6) BbvCI 67 GCTGAGG CCTCAGC(-5/-2) BglI 175 GCCCACGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 144 GGGCTC G[AGT]GC[ACT]^C BpuAI 220 GAAGAC GAAGAC(2/6) BsiEI 105 CGGCCG CG[GA][CT]^CG BsmAI 156 GAGAC GTCTC(1/5) BsmBI 156 GAGACG CGTCTC(1/5) BsoBI 74 CCCGGG C^[CT]CG[GA]G Bsp1286I 144 GGGCTC G[AGT]GC[ACT]^C BspMI 136 ACCTGC ACCTGC(4/8) BssSI 178 CACGAG CACGAG(-5/-1) BstAPI 377 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CfoI 82 GCGC GCG^C DdeI 68 CTGAG C^T[ACGT]AG DpnII 161 GATC ^GATC DsaI 251 CCATGG C^C[GA][CT]GG EagI 105 CGGCCG C^GGCCG EclXI 105 CGGCCG C^GGCCG Eco52I 105 CGGCCG C^GGCCG EcoNI 296 CCTTTTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 112 GAATTC G^AATTC EcoT14I 251 CCATGG C^C[AT][AT]GG FauI 140 GCGGG CCCGC(4/6) HaeII 81 AGCGCC [GA]GCGC^[CT] HhaI 82 GCGC GCG^C HinP1I 82 GCGC G^CGC MaeIII 285 GTCAC ^GT[ACGT]AC MboI 161 GATC ^GATC MluNI 2 TGGCCA TGG^CCA MscI 2 TGGCCA TGG^CCA NcoI 251 CCATGG C^CATGG NdeII 161 GATC ^GATC NspI 11 ACATGC [GA]CATG^[CT] PflFI 279 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 260 CCAGCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RsaI 9 GTAC GT^AC Sau3AI 161 GATC ^GATC SmaI 74 CCCGGG CCC^GGG StuI 293 AGGCCT AGG^CCT StyI 251 CCATGG C^C[AT][AT]GG Tsp45I 285 GTCAC ^GT[GC]AC Tsp509I 113 AATT ^AATT Tth111I 279 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 260 CCAGCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 260 CCAGCGAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 74 CCCGGG C^CCGGG XmaI 74 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 7 enzymes) # ################################### BsiYI 260 CCAGCGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 296 CCTTTTACAGG BslI 260 CCAGCGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 296 CCTTTTACAGG HphI 134 TCACC GGTGA(8/7) 286 TCACC MspA1I 315 CAGCTG C[AC]G^C[GT]G 378 CAGCTG NlaIII 12 CATG CATG^ 252 CATG PvuII 315 CAGCTG CAG^CTG 378 CAGCTG SfaNI 205 GATGC GCATC(5/9) 211 GATGC ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AciI AluI AlwNI BbvI BcnI BsaJI BssKI BstF5I BstNI Cac8I Cfr13I EaeI EcoRII Fnu4HI FokI HaeIII HapII HpaII ItaI MboII MnlI MspI MvaI MwoI NciI NlaIV Sau96I ScrFI TseI