################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanII BbeI BbrPI BcgI BciVI BclI BfrI BlnI BlpI BpmI Bpu1102I BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AcsI 783 GAATTT [GA]^AATT[CT] AflIII 5 ACATGT A^C[GA][CT]GT Aor51HI 383 AGCGCT AGC^GCT ApoI 783 GAATTT [GA]^AATT[CT] AspHI 637 GAGCAC G[AT]GC[AT]^C BbsI 856 GAAGAC GAAGAC(2/6) BbvCI 501 CCTCAGC CCTCAGC(-5/-2) BglI 513 GCCTGAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 374 AGATCT A^GATCT BpuAI 856 GAAGAC GAAGAC(2/6) BsaBI 721 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 262 GAGACC GGTCTC(1/5) BsiHKAI 637 GAGCAC G[AT]GC[AT]^C BsmBI 527 GAGACG CGTCTC(1/5) BspLU11I 5 ACATGT A^CATGT BspMI 737 ACCTGC ACCTGC(4/8) BsrBI 288 CCGCTC CCGCTC(-3/-3) BsrDI 344 GCAATG GCAATG(2/0) BstAPI 732 GCAGTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DrdI 435 GACCAATGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 89 CCGTGG C^C[GA][CT]GG Eco47III 383 AGCGCT AGC^GCT FauI 542 CCCGC CCCGC(4/6) HinfI 859 GACTC G^A[ACGT]TC HphI 94 GGTGA GGTGA(8/7) MamI 721 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboII 856 GAAGA GAAGA(8/7) MseI 944 TTAA T^TAA NheI 318 GCTAGC G^CTAGC NspI 5 ACATGT [GA]CATG^[CT] PleI 859 GACTC GAGTC(4/5) PstI 730 CTGCAG CTGCA^G SfcI 730 CTGCAG C^T[GA][CT]AG SmaI 134 CCCGGG CCC^GGG SmlI 562 CTCAAG C^T[CT][GA]AG StuI 923 AGGCCT AGG^CCT TaqI 920 TCGA T^CGA Tru9I 944 TTAA T^TAA TthHB8I 920 TCGA T^CGA XmaCI 134 CCCGGG C^CCGGG XmaI 134 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AfaI 312 GTAC GT^AC 735 GTAC AlwI 177 GATCC GGATC(4/5) 426 GATCC AlwNI 175 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG 733 CAGTACCTG BanI 61 GGTGCC G^G[CT][GA]CC 806 GGCACC BcnI 134 CCCGG CC^[GC]GG 135 CCGGG BfaI 205 CTAG C^TAG 319 CTAG BmyI 304 GTGCCC G[AGT]GC[ACT]^C 637 GAGCAC Bsp1286I 304 GTGCCC G[AGT]GC[ACT]^C 637 GAGCAC BstF5I 24 CATCC GGATG(2/0) 195 CATCC DraIII 476 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG 863 CACTGGGTG EcoT14I 152 CCAAGG C^C[AT][AT]GG 339 CCTTGG FokI 24 CATCC GGATG(9/13) 195 CATCC HaeII 15 GGCGCT [GA]GCGC^[CT] 383 AGCGCT HapII 135 CCGG C^CGG 323 CCGG HpaII 135 CCGG C^CGG 323 CCGG MaeI 205 CTAG C^TAG 319 CTAG MslI 225 CACTGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 453 CATTTTTATG MspA1I 550 CAGCTG C[AC]G^C[GT]G 571 CAGCTG MspI 135 CCGG C^CGG 323 CCGG NciI 134 CCCGG CC^[GC]GG 135 CCGGG PflMI 380 CCAAGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 651 CCACTGCCTGG PpuMI 107 GGGTCCC [GA]G^G[AT]CC[CT] 818 AGGTCCC PvuII 550 CAGCTG CAG^CTG 571 CAGCTG RsaI 312 GTAC GT^AC 735 GTAC StyI 152 CCAAGG C^C[AT][AT]GG 339 CCTTGG Tsp509I 214 AATT ^AATT 784 AATT Van91I 380 CCAAGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 651 CCACTGCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AluI AvaI AvaII BalI BbvI BsaAI BsaJI BsgI BsiYI BslI BsmAI BsmFI BsoBI BsrI BssKI BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII Fnu4HI HaeIII HhaI HinP1I ItaI MaeII MaeIII MboI MflI MluNI MnlI MscI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TseI Tsp45I TspRI XcmI XhoII