################################## # NO CUT ENZYMES ( 86 enzymes) # ################################## AatII AccI AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI AspEI AspI AvrII BbrPI BcgI BfrI BlnI BlpI Bpu1102I BsiWI Bsp1407I BspDI BspLU11I BsrGI BssSI Bst1107I BstAPI BstBI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoT22I FseI HincII HindII HindIII HpaI KspI MfeI MluI MunI NheI NotI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SbfI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I Van91I XbaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### Acc65I 1496 GGTACC G^GTACC AccIII 1007 TCCGGA T^CCGGA AcyI 619 GGCGCC G[GA]^CG[CT]C Asp700I 1734 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1496 GGTACC G^GTACC AspHI 1074 GTGCTC G[AT]GC[AT]^C BamHI 1841 GGATCC G^GATCC BbeI 619 GGCGCC GGCGC^C BbvCI 448 GCTGAGG CCTCAGC(-5/-2) BfaI 643 CTAG C^TAG BsaHI 619 GGCGCC G[GA]^CG[CT]C BsaWI 1007 TCCGGA [AT]^CCGG[AT] BseAI 1007 TCCGGA T^CCGGA BseRI 1242 CTCCTC GAGGAG(10/8) BsiHKAI 1074 GTGCTC G[AT]GC[AT]^C BspEI 1007 TCCGGA T^CCGGA BssHII 1523 GCGCGC G^CGCGC BstEII 1095 GGTCACC G^GT[ACGT]ACC BstPI 1095 GGTCACC G^GT[ACGT]ACC BstXI 287 CCAGCCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DsaI 725 CCATGG C^C[GA][CT]GG EagI 2042 CGGCCG C^GGCCG EclXI 2042 CGGCCG C^GGCCG Eco52I 2042 CGGCCG C^GGCCG EcoO65I 1095 GGTCACC G^GT[ACGT]ACC EcoRV 97 GATATC GAT^ATC HgaI 1973 GCGTC GACGC(5/10) Hin1I 619 GGCGCC G[GA]^CG[CT]C KasI 619 GGCGCC G^GCGCC KpnI 1496 GGTACC GGTAC^C MaeI 643 CTAG C^TAG MroI 1007 TCCGGA T^CCGGA MseI 1673 TTAA T^TAA NarI 619 GGCGCC GG^CGCC NcoI 725 CCATGG C^CATGG NdeI 102 CATATG CA^TATG NruI 1025 TCGCGA TCG^CGA NspI 1982 GCATGT [GA]CATG^[CT] PaeR7I 1564 CTCGAG C^TCGAG SapI 1467 GCTCTTC GCTCTTC(1/4) SfoI 619 GGCGCC GGC^GCC SmaI 386 CCCGGG CCC^GGG SspI 825 AATATT AAT^ATT TfiI 804 GATTC G^A[AT]TC Tru9I 1673 TTAA T^TAA XhoI 1564 CTCGAG C^TCGAG XmaCI 386 CCCGGG C^CCGGG XmaI 386 CCCGGG C^CCGGG XmnI 1734 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AluI 382 AGCT AG^CT 1590 AGCT AlwNI 1194 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG 1579 CAGCGGCTG ApaI 1319 GGGCCC GGGCC^C 1430 GGGCCC AvaI 386 CCCGGG C^[CT]CG[GA]G 1564 CTCGAG AviII 665 TGCGCA TGC^GCA 1436 TGCGCA BanII 1319 GGGCCC G[GA]GC[CT]^C 1430 GGGCCC BbsI 1147 GTCTTC GAAGAC(2/6) 2064 GTCTTC BclI 791 TGATCA T^GATCA 956 TGATCA BglI 1048 GCCAGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1126 GCCTACTGGGC BpmI 1651 CTGGAG CTGGAG(16/14) 2023 CTGGAG BpuAI 1147 GTCTTC GAAGAC(2/6) 2064 GTCTTC BsaAI 139 TACGTG [CT]AC^GT[GA] 363 CACGTA BsaBI 789 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1695 GATTGCCATC BsaI 173 GAGACC GGTCTC(1/5) 1231 GAGACC BsiEI 351 CGACCG CG[GA][CT]^CG 2042 CGGCCG BsmBI 687 CGTCTC CGTCTC(1/5) 562 GAGACG BsoBI 386 CCCGGG C^[CT]CG[GA]G 1564 CTCGAG BsrBI 1465 CCGCTC CCGCTC(-3/-3) 10 GAGCGG BsrDI 720 CATTGC GCAATG(2/0) 557 GCAATG BsrFI 868 GCCGGC [GA]^CCGG[CT] 1455 GCCGGC Cfr10I 868 GCCGGC [GA]^CCGG[CT] 1455 GCCGGC EcoNI 609 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1724 CCTCGGCCAGG EcoRI 307 GAATTC G^AATTC 1016 GAATTC EcoT14I 500 CCTTGG C^C[AT][AT]GG 725 CCATGG FbaI 791 TGATCA T^GATCA 956 TGATCA FspI 665 TGCGCA TGC^GCA 1436 TGCGCA MamI 789 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1695 GATTGCCATC MspA1I 1579 CAGCGG C[AC]G^C[GT]G 2108 CAGCGG NaeI 868 GCCGGC GCC^GGC 1455 GCCGGC NgoMIV 868 GCCGGC G^CCGGC 1455 GCCGGC PpuMI 69 AGGACCC [GA]G^G[AT]CC[CT] 2018 AGGACCT PstI 1333 CTGCAG CTGCA^G 1592 CTGCAG ScaI 398 AGTACT AGT^ACT 845 AGTACT SexAI 608 ACCTGGT A^CC[AT]GGT 940 ACCTGGT SmlI 1564 CTCGAG C^T[CT][GA]AG 2074 CTCAAG StuI 711 AGGCCT AGG^CCT 2050 AGGCCT StyI 500 CCTTGG C^C[AT][AT]GG 725 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AccII AciI AcsI AfaI AlwI ApoI AvaII BalI BanI BbvI BciVI BcnI BglII BmyI BsaJI BsgI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspHI BspMI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII