(Linear) (Six-base) MAPSORT of: KIEE2606.seq Check: 7690 from: 1 to: 2118 KIEE2606.orf With 182 enzymes: SgfI * February 12, 2016 18:20 .. Acc65I G'GTAC_C Cuts at: 0 1496 2118 Size: 1496 622 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1953 2118 Size: 1953 165 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1984 2016 2118 Size: 1984 32 102 Fragments arranged by size: 1984 102 32 AlwNI CAG_nnn'CTG Cuts at: 0 1199 1584 2118 Size: 1199 385 534 Fragments arranged by size: 1199 534 385 ApaI G_GGCC'C Cuts at: 0 1323 1434 2118 Size: 1323 111 684 Fragments arranged by size: 1323 684 111 ApoI r'AATT_y Cuts at: 0 307 1016 1666 2118 Size: 307 709 650 452 Fragments arranged by size: 709 650 452 307 AvaI C'yCGr_G Cuts at: 0 386 1564 2118 Size: 386 1178 554 Fragments arranged by size: 1178 554 386 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1552 1585 2118 Size: 1552 33 533 Fragments arranged by size: 1552 533 33 BamHI G'GATC_C Cuts at: 0 1841 2118 Size: 1841 277 BanI G'GyrC_C Cuts at: 0 619 1496 1646 1708 2118 Size: 619 877 150 62 410 Fragments arranged by size: 877 619 410 150 62 BanII G_rGCy'C Cuts at: 0 1323 1434 2118 Size: 1323 111 684 Fragments arranged by size: 1323 684 111 BbeI G_GCGC'C Cuts at: 0 623 2118 Size: 623 1495 BbsI GAAGACnn'nnnn_ Cuts at: 0 1140 2057 2118 Size: 1140 917 61 Fragments arranged by size: 1140 917 61 BbvCI CC'TCA_GC Cuts at: 0 449 2118 Size: 449 1669 BciVI GTATCCnnnnn_n' Cuts at: 0 979 1057 1881 2118 Size: 979 78 824 237 Fragments arranged by size: 979 824 237 78 BclI T'GATC_A Cuts at: 0 791 956 2118 Size: 791 165 1162 Fragments arranged by size: 1162 791 165 BglI GCCn_nnn'nGGC Cuts at: 0 1054 1132 2118 Size: 1054 78 986 Fragments arranged by size: 1054 986 78 BglII A'GATC_T Cuts at: 0 329 1181 1337 2118 Size: 329 852 156 781 Fragments arranged by size: 852 781 329 156 Bme1580I G_kGCm'C Cuts at: 0 235 1323 1434 2118 Size: 235 1088 111 684 Fragments arranged by size: 1088 684 235 111 BmrI ACTGGGnnnn_n' Cuts at: 0 1140 1437 1923 2118 Size: 1140 297 486 195 Fragments arranged by size: 1140 486 297 195 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 888 2118 Size: 888 1230 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1672 2044 2118 Size: 1672 372 74 Fragments arranged by size: 1672 372 74 Bpu10I CC'TnA_GC Cuts at: 0 449 1492 2118 Size: 449 1043 626 Fragments arranged by size: 1043 626 449 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2059 2118 Size: 2059 59 BsaI GGTCTCn'nnnn_ Cuts at: 0 167 1225 2118 Size: 167 1058 893 Fragments arranged by size: 1058 893 167 BsaAI yAC'GTr Cuts at: 0 141 365 2118 Size: 141 224 1753 Fragments arranged by size: 1753 224 141 BsaBI GATnn'nnATC Cuts at: 0 793 1699 2118 Size: 793 906 419 Fragments arranged by size: 906 793 419 BsaHI Gr'CG_yC Cuts at: 0 620 2118 Size: 620 1498 BsaWI w'CCGG_w Cuts at: 0 1007 2118 Size: 1007 1111 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1492 1522 2118 Size: 1492 30 596 Fragments arranged by size: 1492 596 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1233 2118 Size: 1233 885 BseYI C'CCAG_C Cuts at: 0 490 813 1326 1386 2118 Size: 490 323 513 60 732 Fragments arranged by size: 732 513 490 323 60 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 75 910 1564 1704 1903 2118 Size: 75 835 654 140 199 215 Fragments arranged by size: 835 654 215 199 140 75 BsiEI CG_ry'CG Cuts at: 0 354 2045 2118 Size: 354 1691 73 Fragments arranged by size: 1691 354 73 BsiHKAI G_wGCw'C Cuts at: 0 1078 2118 Size: 1078 1040 BsmI GAATG_Cn' Cuts at: 0 378 1686 1740 2118 Size: 378 1308 54 378 Fragments arranged by size: 1308 378 378 54 BsmBI CGTCTCn'nnnn_ Cuts at: 0 556 693 2118 Size: 556 137 1425 Fragments arranged by size: 1425 556 137 Bsp1286I G_dGCh'C Cuts at: 0 235 1078 1323 1434 2118 Size: 235 843 245 111 684 Fragments arranged by size: 843 684 245 235 111 BspEI T'CCGG_A Cuts at: 0 1007 2118 Size: 1007 1111 BspHI T'CATG_A Cuts at: 0 1619 1691 1751 2118 Size: 1619 72 60 367 Fragments arranged by size: 1619 367 72 60 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 932 1228 1460 2118 Size: 932 296 232 658 Fragments arranged by size: 932 658 296 232 BsrBI CCG'CTC Cuts at: 0 12 1467 2118 Size: 12 1455 651 Fragments arranged by size: 1455 651 12 BsrDI GCAATG_nn' Cuts at: 0 564 719 2118 Size: 564 155 1399 Fragments arranged by size: 1399 564 155 BsrFI r'CCGG_y Cuts at: 0 868 1455 2118 Size: 868 587 663 Fragments arranged by size: 868 663 587 BssHII G'CGCG_C Cuts at: 0 1523 2118 Size: 1523 595 BstEII G'GTnAC_C Cuts at: 0 1095 2118 Size: 1095 1023 BstXI CCAn_nnnn'nTGG Cuts at: 0 294 2118 Size: 294 1824 BstYI r'GATC_y Cuts at: 0 329 1181 1337 1841 2118 Size: 329 852 156 504 277 Fragments arranged by size: 852 504 329 277 156 BtgI C'CryG_G Cuts at: 0 725 2118 Size: 725 1393 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 745 1008 1107 2118 Size: 745 263 99 1011 Fragments arranged by size: 1011 745 263 99 BtsI GCAGTG_nn' Cuts at: 0 831 1139 2118 Size: 831 308 979 Fragments arranged by size: 979 831 308 EaeI y'GGCC_r Cuts at: 0 125 1367 1403 1482 1535 1727 2042 2118 Size: 125 1242 36 79 53 192 315 76 Fragments arranged by size: 1242 315 192 125 79 76 53 36 EagI C'GGCC_G Cuts at: 0 2042 2118 Size: 2042 76 EarI CTCTTCn'nnn_ Cuts at: 0 1006 1474 1807 2118 Size: 1006 468 333 311 Fragments arranged by size: 1006 468 333 311 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1672 1953 2044 2118 Size: 1672 281 91 74 Fragments arranged by size: 1672 281 91 74 EcoNI CCTnn'n_nnAGG Cuts at: 0 613 1728 2118 Size: 613 1115 390 Fragments arranged by size: 1115 613 390 EcoO109I rG'GnC_Cy Cuts at: 0 70 1320 1409 1431 2019 2118 Size: 70 1250 89 22 588 99 Fragments arranged by size: 1250 588 99 89 70 22 EcoRI G'AATT_C Cuts at: 0 307 1016 2118 Size: 307 709 1102 Fragments arranged by size: 1102 709 307 EcoRV GAT'ATC Cuts at: 0 99 2118 Size: 99 2019 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2100 2118 Size: 2100 18 FspI TGC'GCA Cuts at: 0 667 1438 2118 Size: 667 771 680 Fragments arranged by size: 771 680 667 FspAI rTGC'GCAy Cuts at: 0 667 2118 Size: 667 1451 HaeII r_GCGC'y Cuts at: 0 623 1387 1492 2118 Size: 623 764 105 626 Fragments arranged by size: 764 626 623 105 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1760 1792 2118 Size: 1760 32 326 Fragments arranged by size: 1760 326 32 KasI G'GCGC_C Cuts at: 0 619 2118 Size: 619 1499 KpnI G_GTAC'C Cuts at: 0 1500 2118 Size: 1500 618 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1187 2118 Size: 1187 931 MscI TGG'CCA Cuts at: 0 127 1369 1537 2118 Size: 127 1242 168 581 Fragments arranged by size: 1242 581 168 127 MslI CAynn'nnrTG Cuts at: 0 724 730 799 1555 1768 1987 2118 Size: 724 6 69 756 213 219 131 Fragments arranged by size: 756 724 219 213 131 69 6 MspA1I CmG'CkG Cuts at: 0 1581 2110 2118 Size: 1581 529 8 Fragments arranged by size: 1581 529 8 NaeI GCC'GGC Cuts at: 0 870 1457 2118 Size: 870 587 661 Fragments arranged by size: 870 661 587 NarI GG'CG_CC Cuts at: 0 620 2118 Size: 620 1498 NcoI C'CATG_G Cuts at: 0 725 2118 Size: 725 1393 NdeI CA'TA_TG Cuts at: 0 103 2118 Size: 103 2015 NgoMIV G'CCGG_C Cuts at: 0 868 1455 2118 Size: 868 587 663 Fragments arranged by size: 868 663 587 NruI TCG'CGA Cuts at: 0 1027 2118 Size: 1027 1091 NspI r_CATG'y Cuts at: 0 1986 2118 Size: 1986 132 PfoI T'CCnGG_A Cuts at: 0 589 1778 2118 Size: 589 1189 340 Fragments arranged by size: 1189 589 340 PpuMI rG'GwC_Cy Cuts at: 0 70 2019 2118 Size: 70 1949 99 Fragments arranged by size: 1949 99 70 PspOMI G'GGCC_C Cuts at: 0 1319 1430 2118 Size: 1319 111 688 Fragments arranged by size: 1319 688 111 PspXI vC'TCGA_Gb Cuts at: 0 1564 2118 Size: 1564 554 PstI C_TGCA'G Cuts at: 0 1337 1596 2118 Size: 1337 259 522 Fragments arranged by size: 1337 522 259 SapI GCTCTTCn'nnn_ Cuts at: 0 1474 2118 Size: 1474 644 ScaI AGT'ACT Cuts at: 0 400 847 2118 Size: 400 447 1271 Fragments arranged by size: 1271 447 400 SexAI A'CCwGG_T Cuts at: 0 608 940 2118 Size: 608 332 1178 Fragments arranged by size: 1178 608 332 SfcI C'TryA_G Cuts at: 0 146 1333 1592 2118 Size: 146 1187 259 526 Fragments arranged by size: 1187 526 259 146 SfoI GGC'GCC Cuts at: 0 621 2118 Size: 621 1497 SmaI CCC'GGG Cuts at: 0 388 2118 Size: 388 1730 SmlI C'TyrA_G Cuts at: 0 1564 2074 2118 Size: 1564 510 44 Fragments arranged by size: 1564 510 44 SspI AAT'ATT Cuts at: 0 827 2118 Size: 827 1291 StuI AGG'CCT Cuts at: 0 713 2052 2118 Size: 713 1339 66 Fragments arranged by size: 1339 713 66 StyI C'CwwG_G Cuts at: 0 500 725 2118 Size: 500 225 1393 Fragments arranged by size: 1393 500 225 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 774 1879 2118 Size: 774 1105 239 Fragments arranged by size: 1105 774 239 TatI w'GTAC_w Cuts at: 0 398 845 2118 Size: 398 447 1273 Fragments arranged by size: 1273 447 398 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1492 1524 2118 Size: 1492 32 594 Fragments arranged by size: 1492 594 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 498 1056 1377 2118 Size: 498 558 321 741 Fragments arranged by size: 741 558 498 321 XhoI C'TCGA_G Cuts at: 0 1564 2118 Size: 1564 554 XmaI C'CCGG_G Cuts at: 0 386 2118 Size: 386 1732 XmnI GAAnn'nnTTC Cuts at: 0 1738 2118 Size: 1738 380 Enzymes that do cut: Acc65I AcuI AloI AlwNI ApaI ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BstEII BstXI BstYI BtgI BtgZI BtsI EaeI EagI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NruI NspI PfoI PpuMI PspOMI PspXI PstI SapI ScaI SexAI SfcI SfoI SmaI SmlI SspI StuI StyI TaqII TatI TstI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AhdI AleI AlfI ApaLI AscI AseI AsiSI AvrII BcgI BfrBI BlpI BmgBI BmtI BsiWI BsrGI BssSI BstAPI BstBI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EciI EcoICRI FseI HincII HindIII HpaI MfeI MluI NheI NotI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI ZraI