(Circular) (Six-base) MAPSORT of: pFN21AE6686.seq Check: 1236 from: 1 to: 5551 pFN21AE6686 5551 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3361 278 Size: 53 83 186 936 1825 2468 Fragments arranged by size: 2468 1825 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2894 1937 Size: 957 4594 Acc65I G'GTAC_C Cuts at: 2872 2872 Size: 5551 AclI AA'CG_TT Cuts at: 1563 3678 4051 1563 Size: 2115 373 3063 Fragments arranged by size: 3063 2115 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3614 4998 5266 1632 Size: 1982 1384 268 1917 Fragments arranged by size: 1982 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5354 AflIII A'CryG_T Cuts at: 1203 4450 5427 1203 Size: 3247 977 1327 Fragments arranged by size: 3247 1327 977 AgeI A'CCGG_T Cuts at: 4382 4382 Size: 5551 AhdI GACnn_n'nnGTC Cuts at: 4280 4280 Size: 5551 AleI CACnn'nnGTG Cuts at: 1166 2449 1166 Size: 1283 4268 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5551 AlwNI CAG_nnn'CTG Cuts at: 2349 2708 3294 4866 2349 Size: 359 586 1572 3034 Fragments arranged by size: 3034 1572 586 359 ApaI G_GGCC'C Cuts at: 2553 2607 2553 Size: 54 5497 ApaLI G'TGCA_C Cuts at: 3608 4764 3608 Size: 1156 4395 ApoI r'AATT_y Cuts at: 1493 2860 3086 3238 5347 1493 Size: 1367 226 152 2109 1697 Fragments arranged by size: 2109 1697 1367 226 152 AseI AT'TA_AT Cuts at: 160 1032 4105 160 Size: 872 3073 1606 Fragments arranged by size: 3073 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5551 AvaI C'yCGr_G Cuts at: 1870 1943 2876 1870 Size: 73 933 4545 Fragments arranged by size: 4545 933 73 BamHI G'GATC_C Cuts at: 2881 3248 2881 Size: 367 5184 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2872 4327 618 Size: 325 217 627 1085 1455 1842 Fragments arranged by size: 1842 1455 1085 627 325 217 BanII G_rGCy'C Cuts at: 1391 2053 2114 2415 2499 2553 2607 2770 2870 Size: 662 61 301 84 54 54 163 100 Cuts at: 2870 1391 Size: 4072 Fragments arranged by size: 4072 662 301 163 100 84 61 54 54 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2843 928 Size: 971 944 3636 Fragments arranged by size: 3636 971 944 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3742 3776 1903 Size: 34 1805 34 3678 Fragments arranged by size: 3678 1805 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3443 4653 3443 Size: 1210 4341 BclI T'GATC_A Cuts at: 1548 1548 Size: 5551 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2406 4162 136 Size: 107 122 71 631 605 734 1756 1525 Fragments arranged by size: 1756 1525 734 631 605 122 107 71 BglII A'GATC_T Cuts at: 5546 5546 Size: 5551 BlpI GC'TnA_GC Cuts at: 2426 2968 2426 Size: 542 5009 Bme1580I G_kGCm'C Cuts at: 1790 2168 2211 2553 2607 3612 4768 1790 Size: 378 43 342 54 1005 1156 2573 Fragments arranged by size: 2573 1156 1005 378 342 54 43 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5551 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2798 3282 4240 Size: 416 53 83 399 24 1381 484 958 Cuts at: 4240 442 Size: 1753 Fragments arranged by size: 1753 1381 958 484 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5551 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2117 2470 2585 2639 4211 1239 Size: 878 353 115 54 1572 2579 Fragments arranged by size: 2579 1572 878 353 115 54 Bpu10I CC'TnA_GC Cuts at: 2155 2155 Size: 5551 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3676 4541 4839 3676 Size: 865 298 4388 Fragments arranged by size: 4388 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2658 4214 882 Size: 617 1159 1556 2219 Fragments arranged by size: 2219 1556 1159 617 BsaAI yAC'GTr Cuts at: 493 1204 2451 3342 493 Size: 711 1247 891 2702 Fragments arranged by size: 2702 1247 891 711 BsaBI GATnn'nnATC Cuts at: 5545 5545 Size: 5551 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2340 3358 3740 4375 Size: 53 83 186 936 807 1018 382 635 Cuts at: 4375 275 Size: 1451 Fragments arranged by size: 1451 1018 936 807 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3984 4382 4656 4803 5214 3984 Size: 398 274 147 411 4321 Fragments arranged by size: 4321 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5326 Fragments arranged by size: 5326 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2276 2279 2644 2735 1186 Size: 1090 3 365 91 4002 Fragments arranged by size: 4002 1090 365 91 3 BseYI C'CCAG_C Cuts at: 2150 4754 5250 2150 Size: 2604 496 2451 Fragments arranged by size: 2604 2451 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2290 2317 1163 Size: 573 554 27 4397 Fragments arranged by size: 4397 573 554 27 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3001 3762 4790 1540 Size: 275 186 1000 761 1028 2301 Fragments arranged by size: 2301 1028 1000 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2415 2499 2718 3612 3697 4768 2415 Size: 84 219 894 85 1071 3198 Fragments arranged by size: 3198 1071 894 219 85 84 BsmI GAATG_Cn' Cuts at: 2439 2593 2647 3062 3155 2439 Size: 154 54 415 93 4835 Fragments arranged by size: 4835 415 154 93 54 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2053 2114 2168 2211 2415 2499 2553 Size: 399 263 61 54 43 204 84 54 Cuts at: 2553 2607 2718 2770 2870 3612 3697 4768 1391 Size: 54 111 52 100 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 263 204 111 100 85 84 61 54 54 54 52 43 BspEI T'CCGG_A Cuts at: 5214 5214 Size: 5551 BspHI T'CATG_A Cuts at: 3440 5170 3440 Size: 1730 3821 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2017 2906 844 Size: 1173 889 3489 Fragments arranged by size: 3489 1173 889 BsrBI CCG'CTC Cuts at: 1394 1823 2010 3438 1394 Size: 429 187 1428 3507 Fragments arranged by size: 3507 1428 429 187 BsrDI GCAATG_nn' Cuts at: 65 1248 2306 4046 4220 65 Size: 1183 1058 1740 174 1396 Fragments arranged by size: 1740 1396 1183 1058 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2336 4195 4382 1700 Size: 110 526 1859 187 2869 Fragments arranged by size: 2869 1859 526 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5551 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5551 BssSI C'ACGA_G Cuts at: 2161 3611 4623 2161 Size: 1450 1012 3089 Fragments arranged by size: 3089 1450 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2708 794 Size: 1914 3637 BstBI TT'CG_AA Cuts at: 4355 4355 Size: 5551 BstEII G'GTnAC_C Cuts at: 1184 2731 1184 Size: 1547 4004 BstXI CCAn_nnnn'nTGG Cuts at: 2451 2545 2599 2451 Size: 94 54 5403 Fragments arranged by size: 5403 94 54 BstYI r'GATC_y Cuts at: 1972 2517 2881 3248 3633 3650 5102 5188 5200 Size: 545 364 367 385 17 1452 86 12 Cuts at: 5200 5546 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 545 385 367 364 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2544 2598 513 Size: 552 1479 54 3466 Fragments arranged by size: 3466 1479 552 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2262 2271 2445 487 Size: 683 56 1036 9 174 3593 Fragments arranged by size: 3593 1036 683 174 56 9 BtsI GCAGTG_nn' Cuts at: 3067 3861 3881 3067 Size: 794 20 4737 Fragments arranged by size: 4737 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5551 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5516 DraI TTT'AAA Cuts at: 2855 2855 Size: 5551 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4558 809 Size: 507 3242 1802 Fragments arranged by size: 3242 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2464 2802 2998 Size: 54 1425 50 275 18 634 338 196 Cuts at: 2998 3887 5516 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 634 338 275 196 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2998 1537 Size: 275 1186 4090 Fragments arranged by size: 4090 1186 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3481 1353 Size: 2128 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2771 4138 4512 4658 387 Size: 1064 138 1182 1367 374 146 1280 Fragments arranged by size: 1367 1280 1182 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2413 2497 2413 Size: 84 5467 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2117 2470 2585 2639 3614 4211 4998 Size: 393 485 353 115 54 975 597 787 Cuts at: 4998 5266 1239 Size: 268 1524 Fragments arranged by size: 1524 975 787 597 485 393 353 268 115 54 EcoNI CCTnn'n_nnAGG Cuts at: 2581 2581 Size: 5551 EcoO109I rG'GnC_Cy Cuts at: 2147 2549 2550 2603 2604 2147 Size: 402 1 53 1 5094 Fragments arranged by size: 5094 402 53 1 1 EcoRI G'AATT_C Cuts at: 2860 3238 2860 Size: 378 5173 FspI TGC'GCA Cuts at: 2378 4057 2378 Size: 1679 3872 HaeII r_GCGC'y Cuts at: 1678 2066 2224 2458 2779 4698 1678 Size: 388 158 234 321 1919 2531 Fragments arranged by size: 2531 1919 388 321 234 158 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4192 4224 4266 4298 1373 Size: 32 135 32 2620 32 42 32 2626 Fragments arranged by size: 2626 2620 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2895 3141 669 Size: 988 281 957 246 3079 Fragments arranged by size: 3079 988 957 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5551 HpaI GTT'AAC Cuts at: 3141 3141 Size: 5551 KpnI G_GTAC'C Cuts at: 2876 2876 Size: 5551 MfeI C'AATT_G Cuts at: 3150 3150 Size: 5551 MluI A'CGCG_T Cuts at: 5427 5427 Size: 5551 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1997 2011 4665 4849 1305 Size: 692 14 2654 184 2007 Fragments arranged by size: 2654 2007 692 184 14 MscI TGG'CCA Cuts at: 10 64 1489 1832 2804 10 Size: 54 1425 343 972 2757 Fragments arranged by size: 2757 1425 972 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2449 2543 2597 3509 3868 4027 518 Size: 648 1283 94 54 912 359 159 2042 Fragments arranged by size: 2042 1283 912 648 359 159 94 54 MspA1I CmG'CkG Cuts at: 1606 2705 2967 3644 4792 5037 1606 Size: 1099 262 677 1148 245 2120 Fragments arranged by size: 2120 1148 1099 677 262 245 NcoI C'CATG_G Cuts at: 513 1065 2544 2598 513 Size: 552 1479 54 3466 Fragments arranged by size: 3466 1479 552 54 NdeI CA'TA_TG Cuts at: 387 387 Size: 5551 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5551 NotI GC'GGCC_GC Cuts at: 2998 2998 Size: 5551 NspI r_CATG'y Cuts at: 1132 1229 2909 4454 1132 Size: 97 1680 1545 2229 Fragments arranged by size: 2229 1680 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5551 PciI A'CATG_T Cuts at: 4450 4450 Size: 5551 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5551 PfoI T'CCnGG_A Cuts at: 2523 2523 Size: 5551 PmeI GTTT'AAAC Cuts at: 2855 2855 Size: 5551 PmlI CAC'GTG Cuts at: 2451 2451 Size: 5551 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2035 2067 3620 3652 5133 5165 2035 Size: 32 1553 32 1481 32 2421 Fragments arranged by size: 2421 1553 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5551 PsiI TTA'TAA Cuts at: 3121 3121 Size: 5551 PspOMI G'GGCC_C Cuts at: 2549 2603 2549 Size: 54 5497 PstI C_TGCA'G Cuts at: 830 2246 2387 2903 830 Size: 1416 141 516 3478 Fragments arranged by size: 3478 1416 516 141 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5551 PvuII CAG'CTG Cuts at: 2705 2705 Size: 5551 SacI G_AGCT'C Cuts at: 2415 2499 2415 Size: 84 5467 SalI G'TCGA_C Cuts at: 1936 2893 1936 Size: 957 4594 SbfI CC_TGCA'GG Cuts at: 2903 2903 Size: 5551 ScaI AGT'ACT Cuts at: 3799 5511 3799 Size: 1712 3839 SfcI C'TryA_G Cuts at: 826 1045 2242 2383 2899 4034 4715 4906 826 Size: 219 1197 141 516 1135 681 191 1471 Fragments arranged by size: 1471 1197 1135 681 516 219 191 141 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5551 SgrAI Cr'CCGG_yG Cuts at: 1700 2336 1700 Size: 636 4915 SmaI CCC'GGG Cuts at: 1872 2878 1872 Size: 1006 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3655 4556 4818 820 Size: 197 926 1712 901 262 1553 Fragments arranged by size: 1712 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3342 493 Size: 2849 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5551 SphI G_CATG'C Cuts at: 1132 2909 1132 Size: 1777 3774 SspI AAT'ATT Cuts at: 5 52 3475 5 Size: 47 3423 2081 Fragments arranged by size: 3423 2081 47 StuI AGG'CCT Cuts at: 1517 2094 1517 Size: 577 4974 StyI C'CwwG_G Cuts at: 513 1065 2544 2598 2990 513 Size: 552 1479 54 392 3074 Fragments arranged by size: 3074 1479 552 392 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2185 2484 3578 3595 3748 3933 4378 Size: 1255 605 299 1094 17 153 185 445 Cuts at: 4378 325 Size: 1498 Fragments arranged by size: 1498 1255 1094 605 445 299 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3797 5509 96 Size: 274 80 33 51 1443 1820 1712 138 Fragments arranged by size: 1820 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5511 5543 5511 Size: 32 5519 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5551 XbaI T'CTAG_A Cuts at: 2887 2887 Size: 5551 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5551 XmaI C'CCGG_G Cuts at: 1870 2876 1870 Size: 1006 4545 XmnI GAAnn'nnTTC Cuts at: 3680 3680 Size: 5551 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3359 276 Size: 53 83 186 936 1825 2468 Fragments arranged by size: 2468 1825 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI