################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BbeI BbrPI BbsI BcgI BclI BcnI BfaI BfrI BglI BglII BlnI BpuAI BsaBI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MboII MfeI MflI MluI MroI MseI MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp509I Tth111I Van91I XbaI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccII 81 CGCG CG^CG AcyI 474 GACGCC G[GA]^CG[CT]C Aor51HI 116 AGCGCT AGC^GCT ApaI 231 GGGCCC GGGCC^C BalI 335 TGGCCA TGG^CCA BanI 365 GGCACC G^G[CT][GA]CC BanII 231 GGGCCC G[GA]GC[CT]^C BbvCI 199 CCTCAGC CCTCAGC(-5/-2) BciVI 290 GTATCC GTATCC(6/5) BmyI 231 GGGCCC G[AGT]GC[ACT]^C BpmI 360 CTCCAG CTGGAG(16/14) BsaAI 287 TACGTA [CT]AC^GT[GA] BsaHI 474 GACGCC G[GA]^CG[CT]C BsaI 547 GAGACC GGTCTC(1/5) BsmBI 430 CGTCTC CGTCTC(1/5) Bsp1286I 231 GGGCCC G[AGT]GC[ACT]^C BsrDI 456 CATTGC GCAATG(2/0) BssSI 100 CTCGTG CACGAG(-5/-1) BstUI 81 CGCG CG^CG DpnII 93 GATC ^GATC Eco47III 116 AGCGCT AGC^GCT HapII 4 CCGG C^CGG Hin1I 474 GACGCC G[GA]^CG[CT]C HpaII 4 CCGG C^CGG MboI 93 GATC ^GATC MluNI 335 TGGCCA TGG^CCA MscI 335 TGGCCA TGG^CCA MspI 4 CCGG C^CGG MvnI 81 CGCG CG^CG NcoI 258 CCATGG C^CATGG NdeII 93 GATC ^GATC PaeR7I 148 CTCGAG C^TCGAG PleI 151 GAGTC GAGTC(4/5) PstI 178 CTGCAG CTGCA^G Sau3AI 93 GATC ^GATC SfcI 178 CTGCAG C^T[GA][CT]AG SmlI 148 CTCGAG C^T[CT][GA]AG SnaBI 287 TACGTA TAC^GTA TaqI 149 TCGA T^CGA TthHB8I 149 TCGA T^CGA XcmI 377 CCACCCTGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 148 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AfaI 316 GTAC GT^AC 452 GTAC AlwNI 136 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 466 CAGCGCCTG AvaI 148 CTCGAG C^[CT]CG[GA]G 485 CCCGAG BlpI 130 GCTCAGC GC^T[ACGT]AGC 172 GCTGAGC Bpu1102I 130 GCTCAGC GC^T[ACGT]AGC 172 GCTGAGC BseRI 345 CTCCTC GAGGAG(10/8) 7 GAGGAG BsgI 501 CTGCAC GTGCAG(16/14) 562 GTGCAG BsmAI 547 GAGAC GTCTC(1/5) 431 GTCTC BsoBI 148 CTCGAG C^[CT]CG[GA]G 485 CCCGAG BsrBI 145 CCGCTC CCGCTC(-3/-3) 246 CCGCTC BstAPI 135 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 564 GCAGTGGGTGC BstF5I 111 CATCC GGATG(2/0) 321 CATCC BstXI 294 CCATCGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 374 CCACCACCCTGG CelII 130 GCTCAGC GC^T[ACGT]AGC 172 GCTGAGC DsaI 258 CCATGG C^C[GA][CT]GG 386 CCGTGG EaeI 335 TGGCCA [CT]^GGCC[GA] 383 TGGCCG EcoT14I 258 CCATGG C^C[AT][AT]GG 444 CCAAGG FokI 111 CATCC GGATG(9/13) 321 CATCC HaeII 116 AGCGCT [GA]GCGC^[CT] 467 AGCGCC HphI 90 GGTGA GGTGA(8/7) 327 GGTGA MaeII 167 ACGT A^CGT 288 ACGT MaeIII 153 GTCAC ^GT[ACGT]AC 328 GTGAC MspA1I 139 CAGCTG C[AC]G^C[GT]G 181 CAGCTG NlaIII 259 CATG CATG^ 312 CATG PpuMI 195 AGGACCT [GA]G^G[AT]CC[CT] 206 AGGACCC PvuII 139 CAGCTG CAG^CTG 181 CAGCTG RsaI 316 GTAC GT^AC 452 GTAC StyI 258 CCATGG C^C[AT][AT]GG 444 CCAAGG TfiI 186 GAATC G^A[AT]TC 392 GAATC Tsp45I 153 GTCAC ^GT[GC]AC 328 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AciI AluI AvaII BbvI BsaJI BsiYI BslI BsmFI BsrI BssKI BstNI Cac8I CfoI Cfr13I DdeI DraII EcoO109I EcoRII FauI Fnu4HI HaeIII HgaI HhaI HinP1I HinfI ItaI MnlI MslI MvaI MwoI NlaIV Sau96I ScrFI SfaNI TseI TspRI