(Circular) (Six-base) MAPSORT of: pFN21AE1678.seq Check: 3119 from: 1 to: 5284 pFN21AE1678 5284 bp With 182 enzymes: SgfI * August 16, 2010 14:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3094 278 Size: 53 83 186 936 1558 2468 Fragments arranged by size: 2468 1558 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2627 1937 Size: 690 4594 Acc65I G'GTAC_C Cuts at: 2605 2605 Size: 5284 AclI AA'CG_TT Cuts at: 1563 3411 3784 1563 Size: 1848 373 3063 Fragments arranged by size: 3063 1848 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3347 4731 4999 1632 Size: 1715 1384 268 1917 Fragments arranged by size: 1917 1715 1384 268 AfeI AGC'GCT Cuts at: 2123 2123 Size: 5284 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5087 AflIII A'CryG_T Cuts at: 1203 4183 5160 1203 Size: 2980 977 1327 Fragments arranged by size: 2980 1327 977 AgeI A'CCGG_T Cuts at: 4115 4115 Size: 5284 AhdI GACnn_n'nnGTC Cuts at: 4013 4013 Size: 5284 AleI CACnn'nnGTG Cuts at: 1166 2164 1166 Size: 998 4286 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2444 1184 Size: 1260 4024 AlwNI CAG_nnn'CTG Cuts at: 2146 2476 3027 4599 2146 Size: 330 551 1572 2831 Fragments arranged by size: 2831 1572 551 330 ApaI G_GGCC'C Cuts at: 2240 2240 Size: 5284 ApaLI G'TGCA_C Cuts at: 3341 4497 3341 Size: 1156 4128 ApoI r'AATT_y Cuts at: 1493 2593 2819 2971 5080 1493 Size: 1100 226 152 2109 1697 Fragments arranged by size: 2109 1697 1100 226 152 AseI AT'TA_AT Cuts at: 160 1032 3838 160 Size: 872 2806 1606 Fragments arranged by size: 2806 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5284 AvaI C'yCGr_G Cuts at: 1870 1943 2153 2490 2609 1870 Size: 73 210 337 119 4545 Fragments arranged by size: 4545 337 210 119 73 BamHI G'GATC_C Cuts at: 2614 2981 2614 Size: 367 4917 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2370 2605 4060 618 Size: 325 217 627 583 235 1455 1842 Fragments arranged by size: 1842 1455 627 583 325 235 217 BanII G_rGCy'C Cuts at: 1391 2240 2603 1391 Size: 849 363 4072 Fragments arranged by size: 4072 849 363 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4313 BbvCI CC'TCA_GC Cuts at: 2205 2205 Size: 5284 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3475 3509 1903 Size: 34 50 34 1454 34 3678 Fragments arranged by size: 3678 1454 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2306 3176 4386 2306 Size: 870 1210 3204 Fragments arranged by size: 3204 1210 870 BclI T'GATC_A Cuts at: 1548 1548 Size: 5284 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3895 136 Size: 107 122 71 631 605 2223 1525 Fragments arranged by size: 2223 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5279 5279 Size: 5284 BlpI GC'TnA_GC Cuts at: 2136 2178 2701 2136 Size: 42 523 4719 Fragments arranged by size: 4719 523 42 Bme1580I G_kGCm'C Cuts at: 1790 2240 3345 4501 1790 Size: 450 1105 1156 2573 Fragments arranged by size: 2573 1156 1105 450 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5284 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2255 2529 3015 Size: 416 53 83 399 24 838 274 486 Cuts at: 3015 3973 442 Size: 958 1753 Fragments arranged by size: 1753 958 838 486 416 399 274 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5284 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2350 3944 1239 Size: 1111 1594 2579 Fragments arranged by size: 2579 1594 1111 Bpu10I CC'TnA_GC Cuts at: 2205 2205 Size: 5284 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3409 4274 4572 3409 Size: 865 298 4121 Fragments arranged by size: 4121 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2546 3947 882 Size: 617 1047 1401 2219 Fragments arranged by size: 2219 1401 1047 617 BsaAI yAC'GTr Cuts at: 493 1204 2294 3075 493 Size: 711 1090 781 2702 Fragments arranged by size: 2702 1090 781 711 BsaBI GATnn'nnATC Cuts at: 5278 5278 Size: 5284 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2480 3091 3473 4108 Size: 53 83 186 936 947 611 382 635 Cuts at: 4108 275 Size: 1451 Fragments arranged by size: 1451 947 936 635 611 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3717 4115 4389 4536 4947 3717 Size: 398 274 147 411 4054 Fragments arranged by size: 4054 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5059 Fragments arranged by size: 5059 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2027 2341 1186 Size: 841 314 4129 Fragments arranged by size: 4129 841 314 BseYI C'CCAG_C Cuts at: 2239 2469 4487 4983 2239 Size: 230 2018 496 2540 Fragments arranged by size: 2540 2018 496 230 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2491 2588 1163 Size: 573 755 97 3859 Fragments arranged by size: 3859 755 573 97 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2734 3495 4523 1540 Size: 275 186 733 761 1028 2301 Fragments arranged by size: 2301 1028 761 733 275 186 BsiHKAI G_wGCw'C Cuts at: 3345 3430 4501 3345 Size: 85 1071 4128 Fragments arranged by size: 4128 1071 85 BsmI GAATG_Cn' Cuts at: 2795 2888 2795 Size: 93 5191 BsmBI CGTCTCn'nnnn_ Cuts at: 2441 2441 Size: 5284 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2240 2603 3345 3430 4501 1391 Size: 399 450 363 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 450 399 363 85 BspEI T'CCGG_A Cuts at: 4947 4947 Size: 5284 BspHI T'CATG_A Cuts at: 3173 4903 3173 Size: 1730 3554 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2639 844 Size: 1795 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2152 2253 3171 1394 Size: 429 329 101 918 3507 Fragments arranged by size: 3507 918 429 329 101 BsrDI GCAATG_nn' Cuts at: 65 1248 2460 3779 3953 65 Size: 1183 1212 1319 174 1396 Fragments arranged by size: 1396 1319 1212 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3928 4115 1700 Size: 110 2118 187 2869 Fragments arranged by size: 2869 2118 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5284 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5284 BssSI C'ACGA_G Cuts at: 2105 3344 4356 2105 Size: 1239 1012 3033 Fragments arranged by size: 3033 1239 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2146 2575 794 Size: 1352 429 3503 Fragments arranged by size: 3503 1352 429 BstBI TT'CG_AA Cuts at: 4088 4088 Size: 5284 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5284 BstXI CCAn_nnnn'nTGG Cuts at: 2306 2386 2306 Size: 80 5204 BstYI r'GATC_y Cuts at: 1972 2614 2981 3366 3383 4835 4921 4933 5279 Size: 642 367 385 17 1452 86 12 346 Cuts at: 5279 1972 Size: 1977 Fragments arranged by size: 1977 1452 642 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2263 2391 513 Size: 552 1198 128 3406 Fragments arranged by size: 3406 1198 552 128 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4545 Fragments arranged by size: 4545 683 56 BtsI GCAGTG_nn' Cuts at: 2576 2800 3594 3614 2576 Size: 224 794 20 4246 Fragments arranged by size: 4246 794 224 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5284 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2435 2470 529 Size: 35 1871 35 3343 Fragments arranged by size: 3343 1871 35 35 DraI TTT'AAA Cuts at: 2588 2588 Size: 5284 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4291 809 Size: 507 2975 1802 Fragments arranged by size: 2975 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2340 2388 2731 Size: 54 1425 50 275 18 510 48 343 Cuts at: 2731 3620 5249 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 510 343 275 54 50 48 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2731 1537 Size: 275 919 4090 Fragments arranged by size: 4090 919 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3214 1353 Size: 1861 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3871 4245 4391 387 Size: 1064 138 2282 374 146 1280 Fragments arranged by size: 2282 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2350 3347 3944 4731 4999 1239 Size: 393 718 997 597 787 268 1524 Fragments arranged by size: 1524 997 787 718 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2043 2201 2212 2236 2043 Size: 158 11 24 5091 Fragments arranged by size: 5091 158 24 11 EcoRI G'AATT_C Cuts at: 2593 2971 2593 Size: 378 4906 FspI TGC'GCA Cuts at: 3790 3790 Size: 5284 HaeII r_GCGC'y Cuts at: 1678 2125 2476 4431 1678 Size: 447 351 1955 2531 Fragments arranged by size: 2531 1955 447 351 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3925 3957 3999 4031 1373 Size: 32 135 32 2353 32 42 32 2626 Fragments arranged by size: 2626 2353 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2628 2874 669 Size: 988 281 690 246 3079 Fragments arranged by size: 3079 988 690 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5284 HpaI GTT'AAC Cuts at: 2874 2874 Size: 5284 KpnI G_GTAC'C Cuts at: 2609 2609 Size: 5284 MfeI C'AATT_G Cuts at: 2883 2883 Size: 5284 MluI A'CGCG_T Cuts at: 5160 5160 Size: 5284 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4398 4582 1305 Size: 3093 184 2007 Fragments arranged by size: 3093 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2342 10 Size: 54 1425 343 510 2952 Fragments arranged by size: 2952 1425 510 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2164 2304 2315 2330 3242 3601 3760 Size: 648 998 140 11 15 912 359 159 Cuts at: 3760 518 Size: 2042 Fragments arranged by size: 2042 998 912 648 359 159 140 15 11 MspA1I CmG'CkG Cuts at: 1606 2146 2188 2700 3377 4525 4770 1606 Size: 540 42 512 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 540 512 245 42 NcoI C'CATG_G Cuts at: 513 1065 2263 513 Size: 552 1198 3534 Fragments arranged by size: 3534 1198 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5284 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5284 NotI GC'GGCC_GC Cuts at: 2731 2731 Size: 5284 NspI r_CATG'y Cuts at: 1132 1229 2642 4187 1132 Size: 97 1413 1545 2229 Fragments arranged by size: 2229 1545 1413 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5284 PciI A'CATG_T Cuts at: 4183 4183 Size: 5284 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5284 PmeI GTTT'AAAC Cuts at: 2588 2588 Size: 5284 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3353 3385 4866 4898 3353 Size: 32 1481 32 3739 Fragments arranged by size: 3739 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2201 2212 2201 Size: 11 5273 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5284 PsiI TTA'TAA Cuts at: 2854 2854 Size: 5284 PspOMI G'GGCC_C Cuts at: 2236 2236 Size: 5284 PspXI vC'TCGA_Gb Cuts at: 2153 2153 Size: 5284 PstI C_TGCA'G Cuts at: 830 2187 2636 830 Size: 1357 449 3478 Fragments arranged by size: 3478 1357 449 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5284 PvuII CAG'CTG Cuts at: 2146 2188 2146 Size: 42 5242 SalI G'TCGA_C Cuts at: 1936 2626 1936 Size: 690 4594 SbfI CC_TGCA'GG Cuts at: 2636 2636 Size: 5284 ScaI AGT'ACT Cuts at: 3532 5244 3532 Size: 1712 3572 SfcI C'TryA_G Cuts at: 826 1045 2183 2632 3767 4448 4639 826 Size: 219 1138 449 1135 681 191 1471 Fragments arranged by size: 1471 1138 1135 681 449 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5284 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5284 SmaI CCC'GGG Cuts at: 1872 2611 1872 Size: 739 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2153 3388 4289 4551 820 Size: 197 926 210 1235 901 262 1553 Fragments arranged by size: 1553 1235 926 901 262 210 197 SnaBI TAC'GTA Cuts at: 493 2294 3075 493 Size: 1801 781 2702 Fragments arranged by size: 2702 1801 781 SpeI A'CTAG_T Cuts at: 152 152 Size: 5284 SphI G_CATG'C Cuts at: 1132 2642 1132 Size: 1510 3774 SspI AAT'ATT Cuts at: 5 52 3208 5 Size: 47 3156 2081 Fragments arranged by size: 3156 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5284 StyI C'CwwG_G Cuts at: 513 1065 2263 2449 2723 513 Size: 552 1198 186 274 3074 Fragments arranged by size: 3074 1198 552 274 186 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2040 2154 2563 3311 3328 3481 3666 Size: 1255 460 114 409 748 17 153 185 Cuts at: 3666 4111 325 Size: 445 1498 Fragments arranged by size: 1498 1255 748 460 445 409 185 153 114 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3530 5242 96 Size: 274 80 33 51 1443 1553 1712 138 Fragments arranged by size: 1712 1553 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5244 5276 5244 Size: 32 5252 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5284 XbaI T'CTAG_A Cuts at: 2620 2620 Size: 5284 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2389 2389 Size: 5284 XhoI C'TCGA_G Cuts at: 1943 2153 1943 Size: 210 5074 XmaI C'CCGG_G Cuts at: 1870 2609 1870 Size: 739 4545 XmnI GAAnn'nnTTC Cuts at: 3413 3413 Size: 5284 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3092 276 Size: 53 83 186 936 1558 2468 Fragments arranged by size: 2468 1558 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI