################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstF5I BstYI BstZ17I Bsu36I CelII CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FbaI FokI FseI HincII HindII HindIII HpaI KpnI MaeII MamI MfeI MflI MluI MroI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfaNI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 418 GTCTAC GT^[AC][GT]AC AlwI 238 GATCC GGATC(4/5) AviII 276 TGCGCA TGC^GCA BalI 404 TGGCCA TGG^CCA BanI 691 GGCGCC G^G[CT][GA]CC BbeI 691 GGCGCC GGCGC^C BfaI 439 CTAG C^TAG BpmI 413 CTGGAG CTGGAG(16/14) BseRI 564 CTCCTC GAGGAG(10/8) BsmAI 204 GAGAC GTCTC(1/5) BspDI 307 ATCGAT AT^CGAT BsrI 24 ACTGG ACTGG(1/-1) BstEII 717 GGTGACC G^GT[ACGT]ACC BstPI 717 GGTGACC G^GT[ACGT]ACC BstXI 695 CCACAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 307 ATCGAT AT^CGAT EarI 709 CTCTTC CTCTTC(1/4) EcoNI 258 CCTGCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 717 GGTGACC G^GT[ACGT]ACC FspI 276 TGCGCA TGC^GCA KasI 691 GGCGCC G^GCGCC Ksp632I 709 CTCTTC CTCTTC(1/4) KspI 191 CCGCGG CCGC^GG MaeI 439 CTAG C^TAG MluNI 404 TGGCCA TGG^CCA MscI 404 TGGCCA TGG^CCA MslI 613 CACCTGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 691 GGCGCC GG^CGCC NspI 269 GCATGT [GA]CATG^[CT] PflMI 627 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 216 GGGTCCC [GA]G^G[AT]CC[CT] PstI 499 CTGCAG CTGCA^G PvuII 280 CAGCTG CAG^CTG SacII 191 CCGCGG CCGC^GG SfcI 499 CTGCAG C^T[GA][CT]AG SfoI 691 GGCGCC GGC^GCC StuI 339 AGGCCT AGG^CCT TaqI 308 TCGA T^CGA TthHB8I 308 TCGA T^CGA Van91I 627 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcyI 637 GGCGTC G[GA]^CG[CT]C 691 GGCGCC AfaI 76 GTAC GT^AC 327 GTAC AlwNI 502 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG 628 CAGCGCCTG ApaI 86 GGGCCC GGGCC^C 313 GGGCCC AvaI 201 CCCGAG C^[CT]CG[GA]G 220 CCCGAG BbsI 139 GTCTTC GAAGAC(2/6) 427 GTCTTC BglI 341 GCCTGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 529 GCCGCCCTGGC BpuAI 139 GTCTTC GAAGAC(2/6) 427 GTCTTC BsaHI 637 GGCGTC G[GA]^CG[CT]C 691 GGCGCC BsgI 493 CTGCAC GTGCAG(16/14) 610 CTGCAC BsoBI 201 CCCGAG C^[CT]CG[GA]G 220 CCCGAG BstAPI 501 GCAGCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 664 GCACGCCATGC DpnII 238 GATC ^GATC 306 GATC EcoT14I 82 CCTTGG C^C[AT][AT]GG 407 CCAAGG HaeII 629 AGCGCC [GA]GCGC^[CT] 691 GGCGCC HgaI 16 GACGC GACGC(5/10) 638 GCGTC Hin1I 637 GGCGTC G[GA]^CG[CT]C 691 GGCGCC HphI 717 GGTGA GGTGA(8/7) 748 TCACC MaeIII 332 GTGAC ^GT[ACGT]AC 718 GTGAC MboI 238 GATC ^GATC 306 GATC MspA1I 191 CCGCGG C[AC]G^C[GT]G 280 CAGCTG NdeII 238 GATC ^GATC 306 GATC NlaIII 270 CATG CATG^ 670 CATG RsaI 76 GTAC GT^AC 327 GTAC Sau3AI 238 GATC ^GATC 306 GATC StyI 82 CCTTGG C^C[AT][AT]GG 407 CCAAGG Tsp45I 332 GTGAC ^GT[GC]AC 718 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AccII AciI AluI AvaII BanII BbvI BcnI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BspMI BsrFI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MboII MnlI MspI MvaI MvnI MwoI NaeI NciI NgoMIV NlaIV PleI Sau96I ScrFI TseI TspRI