(Circular) (Six-base) MAPSORT of: pFN21AE1751.seq Check: 254 from: 1 to: 5467 pFN21AE1751 5467 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2511 2511 Size: 5467 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3277 278 Size: 53 83 186 936 1741 2468 Fragments arranged by size: 2468 1741 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2424 2810 1937 Size: 487 386 4594 Fragments arranged by size: 4594 487 386 Acc65I G'GTAC_C Cuts at: 2788 2788 Size: 5467 AclI AA'CG_TT Cuts at: 1563 3594 3967 1563 Size: 2031 373 3063 Fragments arranged by size: 3063 2031 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3530 4914 5182 1632 Size: 1898 1384 268 1917 Fragments arranged by size: 1917 1898 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5270 AflIII A'CryG_T Cuts at: 1203 4366 5343 1203 Size: 3163 977 1327 Fragments arranged by size: 3163 1327 977 AgeI A'CCGG_T Cuts at: 4298 4298 Size: 5467 AhdI GACnn_n'nnGTC Cuts at: 4196 4196 Size: 5467 AleI CACnn'nnGTG Cuts at: 1166 2622 1166 Size: 1456 4011 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2640 1184 Size: 1456 4011 AlwNI CAG_nnn'CTG Cuts at: 2512 2638 3210 4782 2512 Size: 126 572 1572 3197 Fragments arranged by size: 3197 1572 572 126 ApaI G_GGCC'C Cuts at: 2095 2322 2095 Size: 227 5240 ApaLI G'TGCA_C Cuts at: 3524 4680 3524 Size: 1156 4311 ApoI r'AATT_y Cuts at: 1493 2776 3002 3154 5263 1493 Size: 1283 226 152 2109 1697 Fragments arranged by size: 2109 1697 1283 226 152 AseI AT'TA_AT Cuts at: 160 1032 4021 160 Size: 872 2989 1606 Fragments arranged by size: 2989 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5467 AvaI C'yCGr_G Cuts at: 1870 1943 2206 2225 2792 1870 Size: 73 263 19 567 4545 Fragments arranged by size: 4545 567 263 73 19 BamHI G'GATC_C Cuts at: 2797 3164 2797 Size: 367 5100 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2696 2788 4243 618 Size: 325 217 627 909 92 1455 1842 Fragments arranged by size: 1842 1455 909 627 325 217 92 BanII G_rGCy'C Cuts at: 1391 2095 2179 2322 2477 2786 1391 Size: 704 84 143 155 309 4072 Fragments arranged by size: 4072 704 309 155 143 84 BbeI G_GCGC'C Cuts at: 2700 2700 Size: 5467 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2137 2425 928 Size: 971 238 288 3970 Fragments arranged by size: 3970 971 288 238 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3658 3692 1903 Size: 34 1721 34 3678 Fragments arranged by size: 3678 1721 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3359 4569 3359 Size: 1210 4257 BclI T'GATC_A Cuts at: 1548 1548 Size: 5467 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2352 2540 4078 Size: 107 122 71 631 605 680 188 1538 Cuts at: 4078 136 Size: 1525 Fragments arranged by size: 1538 1525 680 631 605 188 122 107 71 BglII A'GATC_T Cuts at: 5462 5462 Size: 5467 BlpI GC'TnA_GC Cuts at: 2884 2884 Size: 5467 Bme1580I G_kGCm'C Cuts at: 1790 2095 2322 2671 3528 4684 1790 Size: 305 227 349 857 1156 2573 Fragments arranged by size: 2573 1156 857 349 305 227 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5467 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3198 4156 442 Size: 416 53 83 399 24 1781 958 1753 Fragments arranged by size: 1781 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5467 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2439 4127 1239 Size: 1200 1688 2579 Fragments arranged by size: 2579 1688 1200 Bpu10I CC'TnA_GC Cuts at: 2324 2375 2476 2324 Size: 51 101 5315 Fragments arranged by size: 5315 101 51 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3592 4457 4755 3592 Size: 865 298 4304 Fragments arranged by size: 4304 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4130 882 Size: 617 2631 2219 Fragments arranged by size: 2631 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3258 493 Size: 711 2054 2702 Fragments arranged by size: 2702 2054 711 BsaBI GATnn'nnATC Cuts at: 5461 5461 Size: 5467 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2643 2697 3274 3656 Size: 53 83 186 936 1110 54 577 382 Cuts at: 3656 4291 275 Size: 635 1451 Fragments arranged by size: 1451 1110 936 635 577 382 186 83 54 53 BsaWI w'CCGG_w Cuts at: 3900 4298 4572 4719 5130 3900 Size: 398 274 147 411 4237 Fragments arranged by size: 4237 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5242 Fragments arranged by size: 5242 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2560 1186 Size: 1374 4093 BseYI C'CCAG_C Cuts at: 2525 2737 4670 5166 2525 Size: 212 1933 496 2826 Fragments arranged by size: 2826 1933 496 212 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2483 2600 1163 Size: 573 747 117 4030 Fragments arranged by size: 4030 747 573 117 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2917 3678 4706 1540 Size: 275 186 916 761 1028 2301 Fragments arranged by size: 2301 1028 916 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3528 3613 4684 3528 Size: 85 1071 4311 Fragments arranged by size: 4311 1071 85 BsmI GAATG_Cn' Cuts at: 2978 3071 2978 Size: 93 5374 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2095 2179 2322 2477 2671 2786 3528 Size: 399 305 84 143 155 194 115 742 Cuts at: 3528 3613 4684 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 305 194 155 143 115 85 84 BspEI T'CCGG_A Cuts at: 5130 5130 Size: 5467 BspHI T'CATG_A Cuts at: 3356 5086 3356 Size: 1730 3737 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2271 2511 2565 2822 844 Size: 1427 240 54 257 3489 Fragments arranged by size: 3489 1427 257 240 54 BsrBI CCG'CTC Cuts at: 1394 1823 3354 1394 Size: 429 1531 3507 Fragments arranged by size: 3507 1531 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3962 4136 65 Size: 1183 2714 174 1396 Fragments arranged by size: 2714 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2249 2465 2627 4111 4298 1700 Size: 110 439 216 162 1484 187 2869 Fragments arranged by size: 2869 1484 439 216 187 162 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5467 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5467 BssSI C'ACGA_G Cuts at: 3527 4539 3527 Size: 1012 4455 BstAPI GCAn_nnn'nTGC Cuts at: 794 2512 2675 794 Size: 1718 163 3586 Fragments arranged by size: 3586 1718 163 BstBI TT'CG_AA Cuts at: 4271 4271 Size: 5467 BstEII G'GTnAC_C Cuts at: 1184 2722 1184 Size: 1538 3929 BstXI CCAn_nnnn'nTGG Cuts at: 2707 2707 Size: 5467 BstYI r'GATC_y Cuts at: 1972 2797 3164 3549 3566 5018 5104 5116 5462 Size: 825 367 385 17 1452 86 12 346 Cuts at: 5462 1972 Size: 1977 Fragments arranged by size: 1977 1452 825 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2045 2196 2550 513 Size: 552 939 41 151 354 3430 Fragments arranged by size: 3430 939 552 354 151 41 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4728 Fragments arranged by size: 4728 683 56 BtsI GCAGTG_nn' Cuts at: 2510 2983 3777 3797 2510 Size: 473 794 20 4180 Fragments arranged by size: 4180 794 473 20 ClaI AT'CG_AT Cuts at: 1554 2313 1554 Size: 759 4708 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5432 DraI TTT'AAA Cuts at: 2771 2771 Size: 5467 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4474 809 Size: 507 3158 1802 Fragments arranged by size: 3158 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2199 2409 2463 Size: 54 1425 50 275 18 369 210 54 Cuts at: 2463 2629 2914 3803 5432 8 Size: 166 285 889 1629 43 Fragments arranged by size: 1629 1425 889 369 285 275 210 166 54 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2914 1537 Size: 275 1102 4090 Fragments arranged by size: 4090 1102 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2720 3397 1353 Size: 1367 677 3423 Fragments arranged by size: 3423 1367 677 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4054 4428 4574 387 Size: 1064 138 2465 374 146 1280 Fragments arranged by size: 2465 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2439 3530 4127 4914 5182 1239 Size: 393 807 1091 597 787 268 1524 Fragments arranged by size: 1524 1091 807 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2267 2267 Size: 5467 EcoO109I rG'GnC_Cy Cuts at: 2092 2222 2319 2371 2092 Size: 130 97 52 5188 Fragments arranged by size: 5188 130 97 52 EcoRI G'AATT_C Cuts at: 2776 3154 2776 Size: 378 5089 FspI TGC'GCA Cuts at: 2283 3973 2283 Size: 1690 3777 HaeII r_GCGC'y Cuts at: 1678 2638 2700 4614 1678 Size: 960 62 1914 2531 Fragments arranged by size: 2531 1914 960 62 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4108 4140 4182 4214 1373 Size: 32 135 32 2536 32 42 32 2626 Fragments arranged by size: 2626 2536 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2811 3057 669 Size: 988 281 873 246 3079 Fragments arranged by size: 3079 988 873 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5467 HpaI GTT'AAC Cuts at: 3057 3057 Size: 5467 KasI G'GCGC_C Cuts at: 2696 2696 Size: 5467 KpnI G_GTAC'C Cuts at: 2792 2792 Size: 5467 MfeI C'AATT_G Cuts at: 3066 3066 Size: 5467 MluI A'CGCG_T Cuts at: 5343 5343 Size: 5467 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2742 4581 4765 1305 Size: 1437 1839 184 2007 Fragments arranged by size: 2007 1839 1437 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2411 10 Size: 54 1425 343 579 3066 Fragments arranged by size: 3066 1425 579 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2622 3425 3784 3943 518 Size: 648 1456 803 359 159 2042 Fragments arranged by size: 2042 1456 803 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2198 2287 2883 3560 4708 4953 1606 Size: 592 89 596 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 596 592 245 89 NaeI GCC'GGC Cuts at: 2251 2467 2629 2251 Size: 216 162 5089 Fragments arranged by size: 5089 216 162 NarI GG'CG_CC Cuts at: 2697 2697 Size: 5467 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3976 Fragments arranged by size: 3976 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5467 NgoMIV G'CCGG_C Cuts at: 2249 2465 2627 2249 Size: 216 162 5089 Fragments arranged by size: 5089 216 162 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5467 NotI GC'GGCC_GC Cuts at: 2914 2914 Size: 5467 NspI r_CATG'y Cuts at: 1132 1229 2278 2825 4370 1132 Size: 97 1049 547 1545 2229 Fragments arranged by size: 2229 1545 1049 547 97 PasI CC'CwG_GG Cuts at: 1348 2160 2692 1348 Size: 812 532 4123 Fragments arranged by size: 4123 812 532 PciI A'CATG_T Cuts at: 4366 4366 Size: 5467 PflMI CCAn_nnn'nTGG Cuts at: 1108 2638 1108 Size: 1530 3937 PmeI GTTT'AAAC Cuts at: 2771 2771 Size: 5467 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3536 3568 5049 5081 3536 Size: 32 1481 32 3922 Fragments arranged by size: 3922 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2222 2222 Size: 5467 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5467 PsiI TTA'TAA Cuts at: 3037 3037 Size: 5467 PspOMI G'GGCC_C Cuts at: 2091 2318 2091 Size: 227 5240 PstI C_TGCA'G Cuts at: 830 2508 2819 830 Size: 1678 311 3478 Fragments arranged by size: 3478 1678 311 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5467 PvuII CAG'CTG Cuts at: 2287 2287 Size: 5467 SacII CC_GC'GG Cuts at: 2199 2199 Size: 5467 SalI G'TCGA_C Cuts at: 1936 2809 1936 Size: 873 4594 SanDI GG'GwC_CC Cuts at: 2222 2222 Size: 5467 SbfI CC_TGCA'GG Cuts at: 2819 2819 Size: 5467 ScaI AGT'ACT Cuts at: 3715 5427 3715 Size: 1712 3755 SfcI C'TryA_G Cuts at: 826 1045 2504 2815 3950 4631 4822 826 Size: 219 1459 311 1135 681 191 1471 Fragments arranged by size: 1471 1459 1135 681 311 219 191 SfoI GGC'GCC Cuts at: 2698 2698 Size: 5467 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5467 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5467 SmaI CCC'GGG Cuts at: 1872 2794 1872 Size: 922 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3571 4472 4734 820 Size: 197 926 1628 901 262 1553 Fragments arranged by size: 1628 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3258 493 Size: 2765 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5467 SphI G_CATG'C Cuts at: 1132 2825 1132 Size: 1693 3774 SspI AAT'ATT Cuts at: 5 52 3391 5 Size: 47 3339 2081 Fragments arranged by size: 3339 2081 47 StuI AGG'CCT Cuts at: 1517 2346 1517 Size: 829 4638 StyI C'CwwG_G Cuts at: 513 1065 2004 2087 2412 2906 513 Size: 552 939 83 325 494 3074 Fragments arranged by size: 3074 939 552 494 325 83 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3494 3511 3664 3849 4294 325 Size: 1255 1914 17 153 185 445 1498 Fragments arranged by size: 1914 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3713 5425 96 Size: 274 80 33 51 1443 1736 1712 138 Fragments arranged by size: 1736 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5427 5459 5427 Size: 32 5435 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5467 XbaI T'CTAG_A Cuts at: 2803 2803 Size: 5467 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5467 XmaI C'CCGG_G Cuts at: 1870 2792 1870 Size: 922 4545 XmnI GAAnn'nnTTC Cuts at: 3596 3596 Size: 5467 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3275 276 Size: 53 83 186 936 1741 2468 Fragments arranged by size: 2468 1741 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SapI SexAI SfiI SrfI SwaI XcmI