(Circular) (Six-base) MAPSORT of: pFN21AE2345.seq Check: 9192 from: 1 to: 5782 pFN21AE2345 5782 bp With 182 enzymes: SgfI * May 20, 2011 16:19 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3592 278 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3125 1937 Size: 1188 4594 Acc65I G'GTAC_C Cuts at: 2913 3048 3103 2913 Size: 135 55 5592 Fragments arranged by size: 5592 135 55 AclI AA'CG_TT Cuts at: 1563 3909 4282 1563 Size: 2346 373 3063 Fragments arranged by size: 3063 2346 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2977 3845 5229 5497 1632 Size: 1345 868 1384 268 1917 Fragments arranged by size: 1917 1384 1345 868 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5585 AflIII A'CryG_T Cuts at: 1203 2661 4681 5658 1203 Size: 1458 2020 977 1327 Fragments arranged by size: 2020 1458 1327 977 AgeI A'CCGG_T Cuts at: 4613 4613 Size: 5782 AhdI GACnn_n'nnGTC Cuts at: 4511 4511 Size: 5782 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5782 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5782 AlwNI CAG_nnn'CTG Cuts at: 2077 2638 2926 3525 5097 2077 Size: 561 288 599 1572 2762 Fragments arranged by size: 2762 1572 599 561 288 ApaI G_GGCC'C Cuts at: 2021 2828 2021 Size: 807 4975 ApaLI G'TGCA_C Cuts at: 2324 3839 4995 2324 Size: 1515 1156 3111 Fragments arranged by size: 3111 1515 1156 ApoI r'AATT_y Cuts at: 1493 3091 3317 3469 5578 1493 Size: 1598 226 152 2109 1697 Fragments arranged by size: 2109 1697 1598 226 152 AseI AT'TA_AT Cuts at: 160 1032 4336 160 Size: 872 3304 1606 Fragments arranged by size: 3304 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 AvaI C'yCGr_G Cuts at: 1870 1943 2374 3107 1870 Size: 73 431 733 4545 Fragments arranged by size: 4545 733 431 73 BamHI G'GATC_C Cuts at: 2008 3112 3479 2008 Size: 1104 367 4311 Fragments arranged by size: 4311 1104 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2254 2913 3048 3103 4558 Size: 325 217 627 467 659 135 55 1455 Cuts at: 4558 618 Size: 1842 Fragments arranged by size: 1842 1455 659 627 467 325 217 135 55 BanII G_rGCy'C Cuts at: 1391 2021 2828 2897 3101 1391 Size: 630 807 69 204 4072 Fragments arranged by size: 4072 807 630 204 69 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4811 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3973 4007 1903 Size: 34 2036 34 3678 Fragments arranged by size: 3678 2036 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2310 2447 3674 4884 2310 Size: 137 1227 1210 3208 Fragments arranged by size: 3208 1227 1210 137 BclI T'GATC_A Cuts at: 1548 1548 Size: 5782 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2798 4393 136 Size: 107 122 71 631 605 1126 1595 1525 Fragments arranged by size: 1595 1525 1126 631 605 122 107 71 BglII A'GATC_T Cuts at: 5777 5777 Size: 5782 BlpI GC'TnA_GC Cuts at: 3199 3199 Size: 5782 Bme1580I G_kGCm'C Cuts at: 1790 2021 2328 2359 2814 2828 3004 3843 4999 Size: 231 307 31 455 14 176 839 1156 Cuts at: 4999 1790 Size: 2573 Fragments arranged by size: 2573 1156 839 455 307 231 176 31 14 BmgBI CAC'GTC Cuts at: 1318 2208 2662 1318 Size: 890 454 4438 Fragments arranged by size: 4438 890 454 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2346 2608 3513 Size: 416 53 83 399 24 929 262 905 Cuts at: 3513 4471 442 Size: 958 1753 Fragments arranged by size: 1753 958 929 905 416 399 262 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5782 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2537 2816 4442 1239 Size: 1298 279 1626 2579 Fragments arranged by size: 2579 1626 1298 279 Bpu10I CC'TnA_GC Cuts at: 2951 2951 Size: 5782 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2100 2636 3907 4772 5070 2100 Size: 536 1271 865 298 2812 Fragments arranged by size: 2812 1271 865 536 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4445 882 Size: 617 2946 2219 Fragments arranged by size: 2946 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2878 3573 493 Size: 711 1674 695 2702 Fragments arranged by size: 2702 1674 711 695 BsaBI GATnn'nnATC Cuts at: 5776 5776 Size: 5782 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2865 3589 3971 4606 Size: 53 83 186 936 1332 724 382 635 Cuts at: 4606 275 Size: 1451 Fragments arranged by size: 1451 1332 936 724 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4215 4613 4887 5034 5445 4215 Size: 398 274 147 411 4552 Fragments arranged by size: 4552 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2510 2540 2657 2687 2699 Size: 30 165 30 1107 30 117 30 12 Cuts at: 2699 2729 1178 Size: 30 4231 Fragments arranged by size: 4231 1107 165 117 30 30 30 30 30 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5782 BseYI C'CCAG_C Cuts at: 2358 2821 2828 2997 4985 5481 2358 Size: 463 7 169 1988 496 2659 Fragments arranged by size: 2659 1988 496 463 169 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5209 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2290 3232 3993 5021 1540 Size: 275 186 289 942 761 1028 2301 Fragments arranged by size: 2301 1028 942 761 289 275 186 BsiHKAI G_wGCw'C Cuts at: 2328 3843 3928 4999 2328 Size: 1515 85 1071 3111 Fragments arranged by size: 3111 1515 1071 85 BsmI GAATG_Cn' Cuts at: 3293 3386 3293 Size: 93 5689 BsmBI CGTCTCn'nnnn_ Cuts at: 2702 2966 2702 Size: 264 5518 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2328 2359 2814 2828 2897 3004 Size: 399 231 307 31 455 14 69 107 Cuts at: 3004 3101 3843 3928 4999 1391 Size: 97 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 455 399 307 231 107 97 85 69 31 14 BspEI T'CCGG_A Cuts at: 5445 5445 Size: 5782 BspHI T'CATG_A Cuts at: 3671 5401 3671 Size: 1730 4052 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2925 3137 844 Size: 2081 212 3489 Fragments arranged by size: 3489 2081 212 BsrBI CCG'CTC Cuts at: 1394 1823 2551 3669 1394 Size: 429 728 1118 3507 Fragments arranged by size: 3507 1118 728 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4277 4451 65 Size: 1183 3029 174 1396 Fragments arranged by size: 3029 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4426 4613 1700 Size: 110 2616 187 2869 Fragments arranged by size: 2869 2616 187 110 BsrGI T'GTAC_A Cuts at: 96 2343 96 Size: 2247 3535 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5782 BssSI C'ACGA_G Cuts at: 3842 4854 3842 Size: 1012 4770 BstAPI GCAn_nnn'nTGC Cuts at: 794 3021 794 Size: 2227 3555 BstBI TT'CG_AA Cuts at: 4586 4586 Size: 5782 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5782 BstXI CCAn_nnnn'nTGG Cuts at: 2905 2905 Size: 5782 BstYI r'GATC_y Cuts at: 1972 2008 2397 3112 3479 3864 3881 5333 5419 Size: 36 389 715 367 385 17 1452 86 Cuts at: 5419 5431 5777 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 715 389 385 367 346 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2445 2522 2814 513 Size: 552 939 441 77 292 3481 Fragments arranged by size: 3481 939 552 441 292 77 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2407 487 Size: 683 56 1181 3862 Fragments arranged by size: 3862 1181 683 56 BtsI GCAGTG_nn' Cuts at: 2280 2425 2595 3298 4092 4112 2280 Size: 145 170 703 794 20 3950 Fragments arranged by size: 3950 794 703 170 145 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5782 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5747 DraI TTT'AAA Cuts at: 3086 3086 Size: 5782 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4789 809 Size: 507 3473 1802 Fragments arranged by size: 3473 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2022 2530 2562 Size: 54 1425 50 275 18 192 508 32 Cuts at: 2562 3229 4118 5747 8 Size: 667 889 1629 43 Fragments arranged by size: 1629 1425 889 667 508 275 192 54 50 43 32 18 EagI C'GGCC_G Cuts at: 1537 1812 3229 1537 Size: 275 1417 4090 Fragments arranged by size: 4090 1417 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3712 1353 Size: 2359 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2602 4369 4743 4889 387 Size: 1064 138 1013 1767 374 146 1280 Fragments arranged by size: 1767 1280 1064 1013 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2537 2816 2977 3845 4442 5229 5497 Size: 393 905 279 161 868 597 787 268 Cuts at: 5497 1239 Size: 1524 Fragments arranged by size: 1524 905 868 787 597 393 279 268 161 EcoNI CCTnn'n_nnAGG Cuts at: 2690 2690 Size: 5782 EcoO109I rG'GnC_Cy Cuts at: 2145 2169 2825 2902 2145 Size: 24 656 77 5025 Fragments arranged by size: 5025 656 77 24 EcoRI G'AATT_C Cuts at: 3091 3469 3091 Size: 378 5404 FspI TGC'GCA Cuts at: 4288 4288 Size: 5782 HaeII r_GCGC'y Cuts at: 1678 2047 4929 1678 Size: 369 2882 2531 Fragments arranged by size: 2882 2531 369 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2510 2542 2699 2731 4423 Size: 32 135 32 938 32 157 32 1692 Cuts at: 4423 4455 4497 4529 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1692 938 157 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3126 3372 669 Size: 988 281 1188 246 3079 Fragments arranged by size: 3079 1188 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5782 HpaI GTT'AAC Cuts at: 3372 3372 Size: 5782 KpnI G_GTAC'C Cuts at: 2917 3052 3107 2917 Size: 135 55 5592 Fragments arranged by size: 5592 135 55 MfeI C'AATT_G Cuts at: 3381 3381 Size: 5782 MluI A'CGCG_T Cuts at: 5658 5658 Size: 5782 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2514 2619 4896 5080 1305 Size: 1209 105 2277 184 2007 Fragments arranged by size: 2277 2007 1209 184 105 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3960 Fragments arranged by size: 3960 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2876 3740 4099 4258 518 Size: 648 1710 864 359 159 2042 Fragments arranged by size: 2042 1710 864 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2074 2241 2437 2997 3198 3875 5023 5268 Size: 468 167 196 560 201 677 1148 245 Cuts at: 5268 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 560 468 245 201 196 167 NcoI C'CATG_G Cuts at: 513 1065 2004 2445 513 Size: 552 939 441 3850 Fragments arranged by size: 3850 939 552 441 NdeI CA'TA_TG Cuts at: 387 387 Size: 5782 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5782 NotI GC'GGCC_GC Cuts at: 3229 3229 Size: 5782 NspI r_CATG'y Cuts at: 1132 1229 3140 4685 1132 Size: 97 1911 1545 2229 Fragments arranged by size: 2229 1911 1545 97 PasI CC'CwG_GG Cuts at: 1348 2014 2692 1348 Size: 666 678 4438 Fragments arranged by size: 4438 678 666 PciI A'CATG_T Cuts at: 4681 4681 Size: 5782 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5782 PmeI GTTT'AAAC Cuts at: 3086 3086 Size: 5782 PmlI CAC'GTG Cuts at: 2878 2878 Size: 5782 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3851 3883 5364 5396 3851 Size: 32 1481 32 4237 Fragments arranged by size: 4237 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2145 2169 2902 2145 Size: 24 733 5025 Fragments arranged by size: 5025 733 24 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5782 PsiI TTA'TAA Cuts at: 3352 3352 Size: 5782 PspOMI G'GGCC_C Cuts at: 2017 2824 2017 Size: 807 4975 PstI C_TGCA'G Cuts at: 830 2643 2922 3036 3134 830 Size: 1813 279 114 98 3478 Fragments arranged by size: 3478 1813 279 114 98 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5782 PvuII CAG'CTG Cuts at: 2074 2437 2997 2074 Size: 363 560 4859 Fragments arranged by size: 4859 560 363 SalI G'TCGA_C Cuts at: 1936 3124 1936 Size: 1188 4594 SanDI GG'GwC_CC Cuts at: 2145 2169 2145 Size: 24 5758 SbfI CC_TGCA'GG Cuts at: 2643 2922 3134 2643 Size: 279 212 5291 Fragments arranged by size: 5291 279 212 ScaI AGT'ACT Cuts at: 4030 5742 4030 Size: 1712 4070 SfcI C'TryA_G Cuts at: 826 1045 2639 2918 3032 3130 4265 4946 5137 Size: 219 1594 279 114 98 1135 681 191 Cuts at: 5137 826 Size: 1471 Fragments arranged by size: 1594 1471 1135 681 279 219 191 114 98 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5782 SmaI CCC'GGG Cuts at: 1872 3109 1872 Size: 1237 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2115 2651 3886 4787 5049 820 Size: 197 926 172 536 1235 901 262 1553 Fragments arranged by size: 1553 1235 926 901 536 262 197 172 SnaBI TAC'GTA Cuts at: 493 3573 493 Size: 3080 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5782 SphI G_CATG'C Cuts at: 1132 3140 1132 Size: 2008 3774 SspI AAT'ATT Cuts at: 5 52 3706 5 Size: 47 3654 2081 Fragments arranged by size: 3654 2081 47 StuI AGG'CCT Cuts at: 1517 2801 3020 1517 Size: 1284 219 4279 Fragments arranged by size: 4279 1284 219 StyI C'CwwG_G Cuts at: 513 1065 2004 2445 2898 3221 513 Size: 552 939 441 453 323 3074 Fragments arranged by size: 3074 939 552 453 441 323 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2559 2585 3809 3826 3979 4164 4609 Size: 1255 979 26 1224 17 153 185 445 Cuts at: 4609 325 Size: 1498 Fragments arranged by size: 1498 1255 1224 979 445 185 153 26 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2343 4028 5740 Size: 274 80 33 51 1443 366 1685 1712 Cuts at: 5740 96 Size: 138 Fragments arranged by size: 1712 1685 1443 366 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5742 5774 5742 Size: 32 5750 Tth111I GACn'n_nGTC Cuts at: 1316 2315 2713 1316 Size: 999 398 4385 Fragments arranged by size: 4385 999 398 XbaI T'CTAG_A Cuts at: 3118 3118 Size: 5782 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2037 2427 2572 2011 Size: 26 390 145 5221 Fragments arranged by size: 5221 390 145 26 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5782 XmaI C'CCGG_G Cuts at: 1870 3107 1870 Size: 1237 4545 XmnI GAAnn'nnTTC Cuts at: 3911 3911 Size: 5782 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3590 276 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI