(Circular) (Six-base) MAPSORT of: pFN21AB0459.seq Check: 1398 from: 1 to: 6229 pFN21AB0459 6229 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3452 3452 Size: 6229 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2505 4039 278 Size: 53 83 186 936 969 1534 2468 Fragments arranged by size: 2468 1534 969 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3572 1937 Size: 1635 4594 Acc65I G'GTAC_C Cuts at: 2265 3327 3550 2265 Size: 1062 223 4944 Fragments arranged by size: 4944 1062 223 AclI AA'CG_TT Cuts at: 1563 4356 4729 1563 Size: 2793 373 3063 Fragments arranged by size: 3063 2793 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4292 5676 5944 1632 Size: 2660 1384 268 1917 Fragments arranged by size: 2660 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6032 AflIII A'CryG_T Cuts at: 1203 5128 6105 1203 Size: 3925 977 1327 Fragments arranged by size: 3925 1327 977 AgeI A'CCGG_T Cuts at: 5060 5060 Size: 6229 AhdI GACnn_n'nnGTC Cuts at: 2061 2964 4958 2061 Size: 903 1994 3332 Fragments arranged by size: 3332 1994 903 AleI CACnn'nnGTG Cuts at: 1166 2286 1166 Size: 1120 5109 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6229 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2420 2452 2420 Size: 32 6197 AlwNI CAG_nnn'CTG Cuts at: 2077 2413 2905 2995 3387 3972 5544 2077 Size: 336 492 90 392 585 1572 2762 Fragments arranged by size: 2762 1572 585 492 392 336 90 ApaI G_GGCC'C Cuts at: 2152 2152 Size: 6229 ApaLI G'TGCA_C Cuts at: 3053 3365 4286 5442 3053 Size: 312 921 1156 3840 Fragments arranged by size: 3840 1156 921 312 ApoI r'AATT_y Cuts at: 1493 3538 3764 3916 6025 1493 Size: 2045 226 152 2109 1697 Fragments arranged by size: 2109 2045 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4783 160 Size: 872 3751 1606 Fragments arranged by size: 3751 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6229 AvaI C'yCGr_G Cuts at: 1870 1943 2152 2389 2423 2961 3185 3344 3444 Size: 73 209 237 34 538 224 159 100 Cuts at: 3444 3554 1870 Size: 110 4545 Fragments arranged by size: 4545 538 237 224 209 159 110 100 73 34 BamHI G'GATC_C Cuts at: 3559 3926 3559 Size: 367 5862 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2106 2156 2265 3181 3327 Size: 325 217 627 319 50 109 916 146 Cuts at: 3327 3464 3550 5005 618 Size: 137 86 1455 1842 Fragments arranged by size: 1842 1455 916 627 325 319 217 146 137 109 86 50 BanII G_rGCy'C Cuts at: 1391 2087 2152 2974 3277 3402 3528 3548 1391 Size: 696 65 822 303 125 126 20 4072 Fragments arranged by size: 4072 822 696 303 126 125 65 20 BbeI G_GCGC'C Cuts at: 3185 3185 Size: 6229 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3250 928 Size: 971 1351 3907 Fragments arranged by size: 3907 1351 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4420 4454 1903 Size: 34 50 34 2399 34 3678 Fragments arranged by size: 3678 2399 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4121 5331 4121 Size: 1210 5019 BclI T'GATC_A Cuts at: 1548 1548 Size: 6229 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2140 2466 4840 Size: 107 122 71 631 605 468 326 2374 Cuts at: 4840 136 Size: 1525 Fragments arranged by size: 2374 1525 631 605 468 326 122 107 71 BglII A'GATC_T Cuts at: 2385 6224 2385 Size: 3839 2390 BlpI GC'TnA_GC Cuts at: 2357 2988 3646 2357 Size: 631 658 4940 Fragments arranged by size: 4940 658 631 Bme1580I G_kGCm'C Cuts at: 1790 2152 2159 3057 3369 3469 4290 5446 1790 Size: 362 7 898 312 100 821 1156 2573 Fragments arranged by size: 2573 1156 898 821 362 312 100 7 BmgBI CAC'GTC Cuts at: 1318 2227 3370 1318 Size: 909 1143 4177 Fragments arranged by size: 4177 1143 909 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2155 3960 4918 Size: 416 53 83 399 24 738 1805 958 Cuts at: 4918 442 Size: 1753 Fragments arranged by size: 1805 1753 958 738 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6229 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2356 3277 2356 Size: 921 5308 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2261 3056 3250 4889 1239 Size: 1022 795 194 1639 2579 Fragments arranged by size: 2579 1639 1022 795 194 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3139 4354 5219 5517 3139 Size: 1215 865 298 3851 Fragments arranged by size: 3851 1215 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2626 3091 4892 882 Size: 617 1127 465 1801 2219 Fragments arranged by size: 2219 1801 1127 617 465 BsaAI yAC'GTr Cuts at: 493 1204 2926 3013 4020 493 Size: 711 1722 87 1007 2702 Fragments arranged by size: 2702 1722 1007 711 87 BsaBI GATnn'nnATC Cuts at: 6223 6223 Size: 6229 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2502 3182 3492 4036 Size: 53 83 186 936 969 680 310 544 Cuts at: 4036 4418 5053 275 Size: 382 635 1451 Fragments arranged by size: 1451 969 936 680 635 544 382 310 186 83 53 BsaWI w'CCGG_w Cuts at: 4662 5060 5334 5481 5892 4662 Size: 398 274 147 411 4999 Fragments arranged by size: 4999 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3311 3341 1178 Size: 30 165 30 1908 30 4066 Fragments arranged by size: 4066 1908 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2681 2684 1186 Size: 1495 3 4731 Fragments arranged by size: 4731 1495 3 BseYI C'CCAG_C Cuts at: 2145 2312 3387 3469 5432 5928 2145 Size: 167 1075 82 1963 496 2446 Fragments arranged by size: 2446 1963 1075 496 167 82 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2507 2602 2879 2993 3480 1163 Size: 573 771 95 277 114 487 3912 Fragments arranged by size: 3912 771 573 487 277 114 95 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2664 2841 3679 4440 5468 1540 Size: 275 186 663 177 838 761 1028 2301 Fragments arranged by size: 2301 1028 838 761 663 275 186 177 BsiHKAI G_wGCw'C Cuts at: 2184 2725 2925 3057 3369 4290 4375 5446 2184 Size: 541 200 132 312 921 85 1071 2967 Fragments arranged by size: 2967 1071 921 541 312 200 132 85 BsmI GAATG_Cn' Cuts at: 3740 3833 3740 Size: 93 6136 BsmBI CGTCTCn'nnnn_ Cuts at: 2125 2828 2882 3484 2125 Size: 703 54 602 4870 Fragments arranged by size: 4870 703 602 54 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2087 2152 2159 2184 2725 2925 2974 Size: 399 297 65 7 25 541 200 49 Cuts at: 2974 3057 3277 3369 3402 3469 3528 3548 4290 Size: 83 220 92 33 67 59 20 742 Cuts at: 4290 4375 5446 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 541 399 297 220 200 92 85 83 67 65 59 49 33 25 20 7 BspEI T'CCGG_A Cuts at: 5892 5892 Size: 6229 BspHI T'CATG_A Cuts at: 2844 3375 4118 5848 2844 Size: 531 743 1730 3225 Fragments arranged by size: 3225 1730 743 531 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2709 3452 3584 844 Size: 1865 743 132 3489 Fragments arranged by size: 3489 1865 743 132 BsrBI CCG'CTC Cuts at: 1394 1823 2329 2987 3139 4116 1394 Size: 429 506 658 152 977 3507 Fragments arranged by size: 3507 977 658 506 429 152 BsrDI GCAATG_nn' Cuts at: 65 1248 4724 4898 65 Size: 1183 3476 174 1396 Fragments arranged by size: 3476 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3159 3408 4873 5060 1700 Size: 110 1349 249 1465 187 2869 Fragments arranged by size: 2869 1465 1349 249 187 110 BsrGI T'GTAC_A Cuts at: 96 2907 96 Size: 2811 3418 BssHII G'CGCG_C Cuts at: 1925 2456 1925 Size: 531 5698 BssSI C'ACGA_G Cuts at: 2861 2975 4289 5301 2861 Size: 114 1314 1012 3789 Fragments arranged by size: 3789 1314 1012 114 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6229 BstBI TT'CG_AA Cuts at: 5033 5033 Size: 6229 BstEII G'GTnAC_C Cuts at: 1184 2230 1184 Size: 1046 5183 BstXI CCAn_nnnn'nTGG Cuts at: 2288 2288 Size: 6229 BstYI r'GATC_y Cuts at: 1972 2385 3559 3926 4311 4328 5780 5866 5878 Size: 413 1174 367 385 17 1452 86 12 Cuts at: 5878 6224 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1174 413 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2059 2369 2377 3315 513 Size: 552 994 310 8 938 3427 Fragments arranged by size: 3427 994 938 552 310 8 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5490 Fragments arranged by size: 5490 683 56 BtsI GCAGTG_nn' Cuts at: 3463 3745 4539 4559 3463 Size: 282 794 20 5133 Fragments arranged by size: 5133 794 282 20 ClaI AT'CG_AT Cuts at: 1554 2547 1554 Size: 993 5236 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6194 DraI TTT'AAA Cuts at: 3533 3533 Size: 6229 DraIII CAC_nnn'GTG Cuts at: 2163 2163 Size: 6229 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5236 809 Size: 507 3920 1802 Fragments arranged by size: 3920 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2494 2533 2661 Size: 54 1425 50 275 18 664 39 128 Cuts at: 2661 2676 2784 2838 3015 3066 3102 3126 3410 Size: 15 108 54 177 51 36 24 284 Cuts at: 3410 3429 3676 4565 6194 8 Size: 19 247 889 1629 43 Fragments arranged by size: 1629 1425 889 664 284 275 247 177 128 108 54 54 51 50 43 39 36 24 19 18 15 EagI C'GGCC_G Cuts at: 1537 1812 2661 2838 3676 1537 Size: 275 849 177 838 4090 Fragments arranged by size: 4090 849 838 275 177 EarI CTCTTCn'nnn_ Cuts at: 1353 2026 4159 1353 Size: 673 2133 3423 Fragments arranged by size: 3423 2133 673 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4816 5190 5336 387 Size: 1064 138 3227 374 146 1280 Fragments arranged by size: 3227 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2261 3056 3250 4292 4889 5676 5944 Size: 393 629 795 194 1042 597 787 268 Cuts at: 5944 1239 Size: 1524 Fragments arranged by size: 1524 1042 795 787 629 597 393 268 194 EcoNI CCTnn'n_nnAGG Cuts at: 2049 2897 2049 Size: 848 5381 EcoO109I rG'GnC_Cy Cuts at: 2055 2149 2335 3094 3359 2055 Size: 94 186 759 265 4925 Fragments arranged by size: 4925 759 265 186 94 EcoRI G'AATT_C Cuts at: 3538 3916 3538 Size: 378 5851 FspI TGC'GCA Cuts at: 4735 4735 Size: 6229 HaeII r_GCGC'y Cuts at: 1678 2146 2321 2613 3185 5376 1678 Size: 468 175 292 572 2191 2531 Fragments arranged by size: 2531 2191 572 468 292 175 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2047 2079 2712 2744 2950 Size: 32 135 32 475 32 633 32 206 Cuts at: 2950 2982 4870 4902 4944 4976 1373 Size: 32 1888 32 42 32 2626 Fragments arranged by size: 2626 1888 633 475 206 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3573 3819 669 Size: 988 281 1635 246 3079 Fragments arranged by size: 3079 1635 988 281 246 HindIII A'AGCT_T Cuts at: 748 3047 748 Size: 2299 3930 HpaI GTT'AAC Cuts at: 3819 3819 Size: 6229 KasI G'GCGC_C Cuts at: 3181 3181 Size: 6229 KpnI G_GTAC'C Cuts at: 2269 3331 3554 2269 Size: 1062 223 4944 Fragments arranged by size: 4944 1062 223 MfeI C'AATT_G Cuts at: 3828 3828 Size: 6229 MluI A'CGCG_T Cuts at: 6105 6105 Size: 6229 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3201 3288 5343 5527 1305 Size: 1896 87 2055 184 2007 Fragments arranged by size: 2055 2007 1896 184 87 MscI TGG'CCA Cuts at: 10 64 1489 1832 2678 3104 3128 10 Size: 54 1425 343 846 426 24 3111 Fragments arranged by size: 3111 1425 846 426 343 54 24 MslI CAynn'nnrTG Cuts at: 518 1166 2286 2579 2915 4187 4546 4705 518 Size: 648 1120 293 336 1272 359 159 2042 Fragments arranged by size: 2042 1272 1120 648 359 336 293 159 MspA1I CmG'CkG Cuts at: 1606 2312 2413 3384 3645 4322 5470 5715 1606 Size: 706 101 971 261 677 1148 245 2120 Fragments arranged by size: 2120 1148 971 706 677 261 245 101 NaeI GCC'GGC Cuts at: 3161 3410 3161 Size: 249 5980 NarI GG'CG_CC Cuts at: 3182 3182 Size: 6229 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5677 NdeI CA'TA_TG Cuts at: 387 3265 387 Size: 2878 3351 NgoMIV G'CCGG_C Cuts at: 3159 3408 3159 Size: 249 5980 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6229 NotI GC'GGCC_GC Cuts at: 3676 3676 Size: 6229 NspI r_CATG'y Cuts at: 1132 1229 3587 5132 1132 Size: 97 2358 1545 2229 Fragments arranged by size: 2358 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6229 PciI A'CATG_T Cuts at: 5128 5128 Size: 6229 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6229 PfoI T'CCnGG_A Cuts at: 2238 2826 2238 Size: 588 5641 PmeI GTTT'AAAC Cuts at: 3533 3533 Size: 6229 PmlI CAC'GTG Cuts at: 2926 3013 2926 Size: 87 6142 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2543 2575 4298 4330 5811 5843 2543 Size: 32 1723 32 1481 32 2929 Fragments arranged by size: 2929 1723 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2055 3094 2055 Size: 1039 5190 PshAI GACnn'nnGTC Cuts at: 1613 2840 1613 Size: 1227 5002 PsiI TTA'TAA Cuts at: 3799 3799 Size: 6229 PspOMI G'GGCC_C Cuts at: 2148 2148 Size: 6229 PstI C_TGCA'G Cuts at: 830 2418 2706 2760 3000 3581 830 Size: 1588 288 54 240 581 3478 Fragments arranged by size: 3478 1588 581 288 240 54 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6229 PvuII CAG'CTG Cuts at: 2312 2413 3384 2312 Size: 101 971 5157 Fragments arranged by size: 5157 971 101 SalI G'TCGA_C Cuts at: 1936 3571 1936 Size: 1635 4594 SanDI GG'GwC_CC Cuts at: 2055 2055 Size: 6229 SbfI CC_TGCA'GG Cuts at: 2760 3581 2760 Size: 821 5408 ScaI AGT'ACT Cuts at: 4477 6189 4477 Size: 1712 4517 SexAI A'CCwGG_T Cuts at: 2868 2940 3096 2868 Size: 72 156 6001 Fragments arranged by size: 6001 156 72 SfcI C'TryA_G Cuts at: 826 1045 2414 2702 2756 2996 3577 4712 5393 Size: 219 1369 288 54 240 581 1135 681 Cuts at: 5393 5584 826 Size: 191 1471 Fragments arranged by size: 1471 1369 1135 681 581 288 240 219 191 54 SfoI GGC'GCC Cuts at: 3183 3183 Size: 6229 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6229 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6229 SmaI CCC'GGG Cuts at: 1872 2154 2963 3446 3556 1872 Size: 282 809 483 110 4545 Fragments arranged by size: 4545 809 483 282 110 SmlI C'TyrA_G Cuts at: 820 1017 1943 3154 4333 5234 5496 820 Size: 197 926 1211 1179 901 262 1553 Fragments arranged by size: 1553 1211 1179 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4020 493 Size: 3527 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6229 SphI G_CATG'C Cuts at: 1132 3587 1132 Size: 2455 3774 SspI AAT'ATT Cuts at: 5 52 4153 5 Size: 47 4101 2081 Fragments arranged by size: 4101 2081 47 StuI AGG'CCT Cuts at: 1517 2246 2822 1517 Size: 729 576 4924 Fragments arranged by size: 4924 729 576 StyI C'CwwG_G Cuts at: 513 1065 3105 3668 513 Size: 552 2040 563 3074 Fragments arranged by size: 3074 2040 563 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2174 4256 4273 4426 4611 5056 325 Size: 1255 594 2082 17 153 185 445 1498 Fragments arranged by size: 2082 1498 1255 594 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2907 4475 6187 Size: 274 80 33 51 1443 930 1568 1712 Cuts at: 6187 96 Size: 138 Fragments arranged by size: 1712 1568 1443 930 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6189 6221 6189 Size: 32 6197 Tth111I GACn'n_nGTC Cuts at: 1316 2228 2474 2656 1316 Size: 912 246 182 4889 Fragments arranged by size: 4889 912 246 182 XbaI T'CTAG_A Cuts at: 3565 3565 Size: 6229 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2142 2142 Size: 6229 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6229 XmaI C'CCGG_G Cuts at: 1870 2152 2961 3444 3554 1870 Size: 282 809 483 110 4545 Fragments arranged by size: 4545 809 483 282 110 XmnI GAAnn'nnTTC Cuts at: 4358 4358 Size: 6229 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2503 4037 276 Size: 53 83 186 936 969 1534 2468 Fragments arranged by size: 2468 1534 969 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacI SacII SapI SfiI SrfI SwaI