Restriction map for
pFN21AB5970
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII Alw44I ApaLI ApoI AseI
AsnI Asp700I Asp718I AspI AvrII
BcgI BciVI BclI BfrI BglII
BlnI BsaBI BsiWI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI DraI DrdI EagI
EarI EclXI Eco52I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
HindIII HpaI KpnI Ksp632I MamI
MluI MseI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PflFI PflMI PmeI PshAI
PshBI Psp1406I PvuI RcaI SalI
SapI SbfI ScaI SfuI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI TfiI Tru9I Tth111I
Van91I XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 1405 GTAGAC GT^[AC][GT]AC
AccIII 948 TCCGGA T^CCGGA
AcyI 1104 GGCGCC G[GA]^CG[CT]C
AgeI 313 ACCGGT A^CCGGT
AhdI 1093 GACCCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 514 CCTGAGG CC^T[ACGT]AGG
AspEI 1093 GACCCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1396 GAGCTC G[AT]GC[AT]^C
AviII 783 TGCGCA TGC^GCA
BbeI 1104 GGCGCC GGCGC^C
BbsI 121 GAAGAC GAAGAC(2/6)
BlpI 1398 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1398 GCTCAGC GC^T[ACGT]AGC
BpuAI 121 GAAGAC GAAGAC(2/6)
BsaHI 1104 GGCGCC G[GA]^CG[CT]C
BseAI 948 TCCGGA T^CCGGA
BsiEI 1491 CGACCG CG[GA][CT]^CG
BsiHKAI 1396 GAGCTC G[AT]GC[AT]^C
BsmBI 874 GAGACG CGTCTC(1/5)
BspEI 948 TCCGGA T^CCGGA
BspMI 8 ACCTGC ACCTGC(4/8)
BsrDI 188 GCAATG GCAATG(2/0)
Bsu36I 514 CCTGAGG CC^T[ACGT]AGG
CelII 1398 GCTCAGC GC^T[ACGT]AGC
CpoI 760 CGGTCCG CG^G[AT]CCG
Eam1105I 1093 GACCCCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 514 CCTGAGG CC^T[ACGT]AGG
FspI 783 TGCGCA TGC^GCA
Hin1I 1104 GGCGCC G[GA]^CG[CT]C
HincII 982 GTCAAC GT[CT]^[GA]AC
HindII 982 GTCAAC GT[CT]^[GA]AC
KasI 1104 GGCGCC G^GCGCC
MfeI 1458 CAATTG C^AATTG
MroI 948 TCCGGA T^CCGGA
MslI 726 CACACGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1458 CAATTG C^AATTG
NaeI 961 GCCGGC GCC^GGC
NarI 1104 GGCGCC GG^CGCC
NgoMIV 961 GCCGGC G^CCGGC
NlaIII 1653 CATG CATG^
PaeR7I 838 CTCGAG C^TCGAG
PinAI 313 ACCGGT A^CCGGT
RsrII 760 CGGTCCG CG^G[AT]CCG
SacI 1396 GAGCTC GAGCT^C
SexAI 584 ACCTGGT A^CC[AT]GGT
SfoI 1104 GGCGCC GGC^GCC
SgrAI 312 CACCGGTG C[GA]^CCGG[CT]G
SmlI 838 CTCGAG C^T[CT][GA]AG
StuI 242 AGGCCT AGG^CCT
Tsp509I 1459 AATT ^AATT
XhoI 838 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Aor51HI 1269 AGCGCT AGC^GCT
1421 AGCGCT
ApaI 266 GGGCCC GGGCC^C
276 GGGCCC
BalI 290 TGGCCA TGG^CCA
1658 TGGCCA
BbrPI 1516 CACGTG CAC^GTG
1642 CACGTG
BbvCI 435 GCTGAGG CCTCAGC(-5/-2)
1047 GCTGAGG
BfaI 31 CTAG C^TAG
979 CTAG
BsaAI 1516 CACGTG [CT]AC^GT[GA]
1642 CACGTG
BsaI 284 GAGACC GGTCTC(1/5)
1441 GAGACC
BsaWI 313 ACCGGT [AT]^CCGG[AT]
948 TCCGGA
BsgI 1087 CTGCAC GTGCAG(16/14)
259 GTGCAG
BsrFI 313 ACCGGT [GA]^CCGG[CT]
961 GCCGGC
BstXI 1115 CCACGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1649 CCAACATGGTGG
Cfr10I 313 ACCGGT [GA]^CCGG[CT]
961 GCCGGC
Eco47III 1269 AGCGCT AGC^GCT
1421 AGCGCT
EcoNI 431 CCTCGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1614 CCTGTCGGAGG
HgaI 876 GACGC GACGC(5/10)
952 GACGC
HinfI 664 GACTC G^A[ACGT]TC
844 GAGTC
HphI 316 GGTGA GGTGA(8/7)
17 TCACC
MaeI 31 CTAG C^TAG
979 CTAG
MluNI 290 TGGCCA TGG^CCA
1658 TGGCCA
MscI 290 TGGCCA TGG^CCA
1658 TGGCCA
PleI 664 GACTC GAGTC(4/5)
844 GAGTC
PmaCI 1516 CACGTG CAC^GTG
1642 CACGTG
PmlI 1516 CACGTG CAC^GTG
1642 CACGTG
PvuII 89 CAGCTG CAG^CTG
793 CAGCTG
SfaNI 1536 GATGC GCATC(5/9)
1628 GCATC
SfiI 1240 GGCCCCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1542 GGCCCGTGGGGCC
SmaI 917 CCCGGG CCC^GGG
1179 CCCGGG
TaqI 839 TCGA T^CGA
1019 TCGA
TthHB8I 839 TCGA T^CGA
1019 TCGA
XmaCI 917 CCCGGG C^CCGGG
1179 CCCGGG
XmaI 917 CCCGGG C^CCGGG
1179 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AscI AvaI AvaII BamHI
BanI BanII BbvI BcnI BglI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssHII BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HpaII ItaI KspI
MaeII MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIV PpuMI
PstI RsaI SacII Sau3AI Sau96I
ScrFI SfcI TseI Tsp45I TspRI
XhoII