(Linear) (Six-base) MAPSORT of: KIBB5970.seq Check: 3160 from: 1 to: 1680 KIBB5970.orf With 182 enzymes: SgfI * July 15, 2010 18:30 .. AccI GT'mk_AC Cuts at: 0 1406 1680 Size: 1406 274 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1303 1552 1680 Size: 1303 249 128 Fragments arranged by size: 1303 249 128 AfeI AGC'GCT Cuts at: 0 1271 1423 1680 Size: 1271 152 257 Fragments arranged by size: 1271 257 152 AgeI A'CCGG_T Cuts at: 0 313 1680 Size: 313 1367 AhdI GACnn_n'nnGTC Cuts at: 0 1098 1680 Size: 1098 582 AlwNI CAG_nnn'CTG Cuts at: 0 387 696 1299 1680 Size: 387 309 603 381 Fragments arranged by size: 603 387 381 309 ApaI G_GGCC'C Cuts at: 0 270 280 1680 Size: 270 10 1400 Fragments arranged by size: 1400 270 10 AscI GG'CGCG_CC Cuts at: 0 1126 1213 1561 1680 Size: 1126 87 348 119 Fragments arranged by size: 1126 348 119 87 AvaI C'yCGr_G Cuts at: 0 281 569 838 917 1179 1438 1680 Size: 281 288 269 79 262 259 242 Fragments arranged by size: 288 281 269 262 259 242 79 BamHI G'GATC_C Cuts at: 0 945 1074 1323 1680 Size: 945 129 249 357 Fragments arranged by size: 945 357 249 129 BanI G'GyrC_C Cuts at: 0 97 547 1104 1680 Size: 97 450 557 576 Fragments arranged by size: 576 557 450 97 BanII G_rGCy'C Cuts at: 0 54 225 270 280 452 770 1400 1680 Size: 54 171 45 10 172 318 630 280 Fragments arranged by size: 630 318 280 172 171 54 45 10 BbeI G_GCGC'C Cuts at: 0 1108 1680 Size: 1108 572 BbsI GAAGACnn'nnnn_ Cuts at: 0 128 1680 Size: 128 1552 BbvCI CC'TCA_GC Cuts at: 0 436 1048 1680 Size: 436 612 632 Fragments arranged by size: 632 612 436 BglI GCCn_nnn'nGGC Cuts at: 0 49 822 1238 1247 1549 1558 1680 Size: 49 773 416 9 302 9 122 Fragments arranged by size: 773 416 302 122 49 9 9 BlpI GC'TnA_GC Cuts at: 0 1399 1680 Size: 1399 281 Bme1580I G_kGCm'C Cuts at: 0 100 270 280 552 809 1347 1583 1649 Size: 100 170 10 272 257 538 236 66 Cuts at: 1649 1680 Size: 31 Fragments arranged by size: 538 272 257 236 170 100 66 31 10 BmgBI CAC'GTC Cuts at: 0 1100 1680 Size: 1100 580 BmrI ACTGGGnnnn_n' Cuts at: 0 184 1680 Size: 184 1496 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 122 424 479 892 1027 1680 Size: 122 302 55 413 135 653 Fragments arranged by size: 653 413 302 135 122 55 Bpu10I CC'TnA_GC Cuts at: 0 436 1048 1680 Size: 436 612 632 Fragments arranged by size: 632 612 436 BsaI GGTCTCn'nnnn_ Cuts at: 0 278 1435 1680 Size: 278 1157 245 Fragments arranged by size: 1157 278 245 BsaAI yAC'GTr Cuts at: 0 1518 1644 1680 Size: 1518 126 36 Fragments arranged by size: 1518 126 36 BsaHI Gr'CG_yC Cuts at: 0 1105 1680 Size: 1105 575 BsaWI w'CCGG_w Cuts at: 0 313 948 1680 Size: 313 635 732 Fragments arranged by size: 732 635 313 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 97 476 856 1042 1636 1680 Size: 97 379 380 186 594 44 Fragments arranged by size: 594 380 379 186 97 44 BseYI C'CCAG_C Cuts at: 0 57 272 351 535 853 1680 Size: 57 215 79 184 318 827 Fragments arranged by size: 827 318 215 184 79 57 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 280 1072 1680 Size: 280 792 608 Fragments arranged by size: 792 608 280 BsiEI CG_ry'CG Cuts at: 0 1494 1680 Size: 1494 186 BsiHKAI G_wGCw'C Cuts at: 0 1400 1680 Size: 1400 280 BsmBI CGTCTCn'nnnn_ Cuts at: 0 868 1680 Size: 868 812 Bsp1286I G_dGCh'C Cuts at: 0 54 100 225 270 280 452 552 770 Size: 54 46 125 45 10 172 100 218 Cuts at: 770 809 1347 1400 1583 1649 1680 Size: 39 538 53 183 66 31 Fragments arranged by size: 538 218 183 172 125 100 66 54 53 46 45 39 31 10 BspEI T'CCGG_A Cuts at: 0 948 1680 Size: 948 732 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 17 1680 Size: 17 1663 BsrDI GCAATG_nn' Cuts at: 0 195 1680 Size: 195 1485 BsrFI r'CCGG_y Cuts at: 0 313 961 1680 Size: 313 648 719 Fragments arranged by size: 719 648 313 BssHII G'CGCG_C Cuts at: 0 1126 1213 1385 1561 1634 1680 Size: 1126 87 172 176 73 46 Fragments arranged by size: 1126 176 172 87 73 46 BstAPI GCAn_nnn'nTGC Cuts at: 0 696 1299 1644 1680 Size: 696 603 345 36 Fragments arranged by size: 696 603 345 36 BstXI CCAn_nnnn'nTGG Cuts at: 0 1122 1656 1680 Size: 1122 534 24 Fragments arranged by size: 1122 534 24 BstYI r'GATC_y Cuts at: 0 945 1074 1323 1680 Size: 945 129 249 357 Fragments arranged by size: 945 357 249 129 Bsu36I CC'TnA_GG Cuts at: 0 515 1680 Size: 515 1165 BtgI C'CryG_G Cuts at: 0 654 757 830 998 1108 1115 1545 1680 Size: 654 103 73 168 110 7 430 135 Fragments arranged by size: 654 430 168 135 110 103 73 7 BtsI GCAGTG_nn' Cuts at: 0 694 1055 1680 Size: 694 361 625 Fragments arranged by size: 694 625 361 DraIII CAC_nnn'GTG Cuts at: 0 1521 1644 1667 1680 Size: 1521 123 23 13 Fragments arranged by size: 1521 123 23 13 EaeI y'GGCC_r Cuts at: 0 290 789 959 1061 1193 1658 1680 Size: 290 499 170 102 132 465 22 Fragments arranged by size: 499 465 290 170 132 102 22 EcoICRI GAG'CTC Cuts at: 0 1398 1680 Size: 1398 282 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 122 424 479 892 1027 1303 1552 1680 Size: 122 302 55 413 135 276 249 128 Fragments arranged by size: 413 302 276 249 135 128 122 55 EcoNI CCTnn'n_nnAGG Cuts at: 0 435 1618 1680 Size: 435 1183 62 Fragments arranged by size: 1183 435 62 EcoO109I rG'GnC_Cy Cuts at: 0 6 69 266 276 277 356 369 614 Size: 6 63 197 10 1 79 13 245 Cuts at: 614 906 975 1240 1296 1680 Size: 292 69 265 56 384 Fragments arranged by size: 384 292 265 245 197 79 69 63 56 13 10 6 1 FspI TGC'GCA Cuts at: 0 785 1680 Size: 785 895 HaeII r_GCGC'y Cuts at: 0 233 387 487 901 1006 1014 1108 1273 Size: 233 154 100 414 105 8 94 165 Cuts at: 1273 1291 1425 1680 Size: 18 134 255 Fragments arranged by size: 414 255 233 165 154 134 105 100 94 18 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1084 1116 1680 Size: 1084 32 564 Fragments arranged by size: 1084 564 32 HincII GTy'rAC Cuts at: 0 984 1680 Size: 984 696 KasI G'GCGC_C Cuts at: 0 1104 1680 Size: 1104 576 MfeI C'AATT_G Cuts at: 0 1458 1680 Size: 1458 222 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 971 1514 1598 1680 Size: 971 543 84 82 Fragments arranged by size: 971 543 84 82 MscI TGG'CCA Cuts at: 0 292 1660 1680 Size: 292 1368 20 Fragments arranged by size: 1368 292 20 MslI CAynn'nnrTG Cuts at: 0 730 1680 Size: 730 950 MspA1I CmG'CkG Cuts at: 0 91 272 656 719 759 777 795 832 Size: 91 181 384 63 40 18 18 37 Cuts at: 832 1000 1035 1206 1680 Size: 168 35 171 474 Fragments arranged by size: 474 384 181 171 168 91 63 40 37 35 18 18 NaeI GCC'GGC Cuts at: 0 963 1680 Size: 963 717 NarI GG'CG_CC Cuts at: 0 1105 1680 Size: 1105 575 NgoMIV G'CCGG_C Cuts at: 0 961 1680 Size: 961 719 PasI CC'CwG_GG Cuts at: 0 45 373 502 1680 Size: 45 328 129 1178 Fragments arranged by size: 1178 328 129 45 PfoI T'CCnGG_A Cuts at: 0 443 869 1680 Size: 443 426 811 Fragments arranged by size: 811 443 426 PmlI CAC'GTG Cuts at: 0 1518 1644 1680 Size: 1518 126 36 Fragments arranged by size: 1518 126 36 PpuMI rG'GwC_Cy Cuts at: 0 6 369 906 1680 Size: 6 363 537 774 Fragments arranged by size: 774 537 363 6 PspOMI G'GGCC_C Cuts at: 0 266 276 1680 Size: 266 10 1404 Fragments arranged by size: 1404 266 10 PspXI vC'TCGA_Gb Cuts at: 0 838 1680 Size: 838 842 PstI C_TGCA'G Cuts at: 0 650 692 1295 1680 Size: 650 42 603 385 Fragments arranged by size: 650 603 385 42 PvuII CAG'CTG Cuts at: 0 91 795 1680 Size: 91 704 885 Fragments arranged by size: 885 704 91 RsrII CG'GwC_CG Cuts at: 0 761 1680 Size: 761 919 SacI G_AGCT'C Cuts at: 0 1400 1680 Size: 1400 280 SacII CC_GC'GG Cuts at: 0 657 760 833 1001 1680 Size: 657 103 73 168 679 Fragments arranged by size: 679 657 168 103 73 SanDI GG'GwC_CC Cuts at: 0 369 906 1680 Size: 369 537 774 Fragments arranged by size: 774 537 369 SexAI A'CCwGG_T Cuts at: 0 584 1680 Size: 584 1096 SfcI C'TryA_G Cuts at: 0 646 688 1291 1680 Size: 646 42 603 389 Fragments arranged by size: 646 603 389 42 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1247 1549 1680 Size: 1247 302 131 Fragments arranged by size: 1247 302 131 SfoI GGC'GCC Cuts at: 0 1106 1680 Size: 1106 574 SgrAI Cr'CCGG_yG Cuts at: 0 313 1680 Size: 313 1367 SmaI CCC'GGG Cuts at: 0 919 1181 1680 Size: 919 262 499 Fragments arranged by size: 919 499 262 SmlI C'TyrA_G Cuts at: 0 838 1680 Size: 838 842 SrfI GCCC'GGGC Cuts at: 0 919 1680 Size: 919 761 StuI AGG'CCT Cuts at: 0 244 1680 Size: 244 1436 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 867 1680 Size: 867 813 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1059 1091 1680 Size: 1059 32 589 Fragments arranged by size: 1059 589 32 XhoI C'TCGA_G Cuts at: 0 838 1680 Size: 838 842 XmaI C'CCGG_G Cuts at: 0 917 1179 1680 Size: 917 262 501 Fragments arranged by size: 917 501 262 Enzymes that do cut: AccI AcuI AfeI AgeI AhdI AlwNI ApaI AscI AvaI BamHI BanI BanII BbeI BbsI BbvCI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrDI BsrFI BssHII BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII KasI MfeI MmeI MscI MslI MspA1I NaeI NarI NgoMIV PasI PfoI PmlI PpuMI PspOMI PspXI PstI PvuII RsrII SacI SacII SanDI SexAI SfcI SfiI SfoI SgrAI SmaI SmlI SrfI StuI TaqII TstI XhoI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AflIII AleI AlfI AloI ApaLI ApoI AseI AsiSI AvrII BaeI BcgI BciVI BclI BfrBI BglII BmtI BplI BpuEI BsaBI BsaXI BsiWI BsmI BspHI BsrBI BsrGI BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EarI EciI EcoRI EcoRV FalI FseI FspAI HindIII HpaI KpnI MluI NcoI NdeI NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PpiI PshAI PsiI PsrI PvuI SalI SapI SbfI ScaI SgfI SnaBI SpeI SphI SspI StyI SwaI TatI Tth111I XbaI XcmI XmnI ZraI