################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AvrII BbrPI BbvCI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HinfI HpaI KpnI Ksp632I MaeI MamI MfeI MluI MroI MseI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SapI SbfI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AflIII 404 ACACGT A^C[GA][CT]GT AluI 527 AGCT AG^CT AlwNI 736 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 128 AGCGCT AGC^GCT ApaI 305 GGGCCC GGGCC^C AspI 277 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 101 TGCGCA TGC^GCA BalI 212 TGGCCA TGG^CCA BamHI 392 GGATCC G^GATCC BanII 305 GGGCCC G[GA]GC[CT]^C BbsI 283 GTCTTC GAAGAC(2/6) BcgI 209 GCATGGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BmyI 305 GGGCCC G[AGT]GC[ACT]^C BpuAI 283 GTCTTC GAAGAC(2/6) BsaAI 405 CACGTA [CT]AC^GT[GA] BsgI 232 GTGCAG GTGCAG(16/14) Bsp1286I 305 GGGCCC G[AGT]GC[ACT]^C BspMI 98 ACCTGC ACCTGC(4/8) BsrDI 272 GCAATG GCAATG(2/0) BsrFI 117 GCCGGT [GA]^CCGG[CT] BstAPI 735 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 773 CCAGCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 392 GGATCC [GA]^GATC[CT] Cfr10I 117 GCCGGT [GA]^CCGG[CT] Eco47III 128 AGCGCT AGC^GCT EcoNI 345 CCTGTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 101 TGCGCA TGC^GCA KspI 71 CCGCGG CCGC^GG MaeII 406 ACGT A^CGT MflI 392 GGATCC [GA]^GATC[CT] MluNI 212 TGGCCA TGG^CCA MscI 212 TGGCCA TGG^CCA NcoI 591 CCATGG C^CATGG PflFI 277 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 45 CTGCAG CTGCA^G SacII 71 CCGCGG CCGC^GG ScaI 542 AGTACT AGT^ACT SfaNI 104 GCATC GCATC(5/9) SfcI 45 CTGCAG C^T[GA][CT]AG StuI 342 AGGCCT AGG^CCT TaqI 266 TCGA T^CGA Tth111I 277 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 266 TCGA T^CGA XhoII 392 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AvaII 189 GGTCC G^G[AT]CC 664 GGTCC BbeI 66 GGCGCC GGCGC^C 240 GGCGCC BseRI 171 CTCCTC GAGGAG(10/8) 421 GAGGAG BsmFI 299 GGGAC GGGAC(10/14) 665 GTCCC BsrI 302 ACTGG ACTGG(1/-1) 441 CCAGT DdeI 546 CTCAG C^T[ACGT]AG 758 CTCAG DpnII 261 GATC ^GATC 393 GATC EaeI 158 TGGCCG [CT]^GGCC[GA] 212 TGGCCA EcoT14I 591 CCATGG C^C[AT][AT]GG 612 CCTTGG FauI 52 CCCGC CCCGC(4/6) 70 CCCGC KasI 66 GGCGCC G^GCGCC 240 GGCGCC MaeIII 380 GTCAC ^GT[ACGT]AC 608 GTGAC MboI 261 GATC ^GATC 393 GATC MboII 26 TCTTC GAAGA(8/7) 284 TCTTC MslI 382 CACTTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 555 CACCATCATG NarI 66 GGCGCC GG^CGCC 240 GGCGCC NdeII 261 GATC ^GATC 393 GATC PpuMI 188 GGGTCCT [GA]G^G[AT]CC[CT] 663 GGGTCCC Sau3AI 261 GATC ^GATC 393 GATC SfoI 66 GGCGCC GGC^GCC 240 GGCGCC StyI 591 CCATGG C^C[AT][AT]GG 612 CCTTGG Tsp45I 380 GTCAC ^GT[GC]AC 608 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AccII AciI AcyI AfaI AlwI BanI BbvI BcnI BpmI BsaHI BsaI BsaJI BsiYI BslI BsmAI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DraII DsaI EcoO109I EcoRII Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV RsaI Sau96I ScrFI TseI TspRI