################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvrII BalI BamHI BbrPI BbsI BciVI BclI BfaI BfrI BlnI BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstXI BstZ17I Bsu36I ClaI DraI DrdI Eam1105I EarI Eco81I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI Ksp632I MaeI MamI MboII MfeI MluI MluNI MroI MscI MunI NcoI NdeI NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI SalI SapI ScaI SexAI SfaNI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI StuI SwaI TaqI TfiI Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AcyI 118 GGCGCC G[GA]^CG[CT]C AlwNI 466 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 560 AGCGCT AGC^GCT AspHI 640 GAGCTC G[AT]GC[AT]^C AviII 242 TGCGCA TGC^GCA BbeI 118 GGCGCC GGCGC^C BbvCI 1045 GCTGAGG CCTCAGC(-5/-2) BcgI 620 CGATGAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 397 GCCTGTACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 881 AGATCT A^GATCT BlpI 642 GCTCAGC GC^T[ACGT]AGC Bpu1102I 642 GCTCAGC GC^T[ACGT]AGC BsaHI 118 GGCGCC G[GA]^CG[CT]C BsiEI 263 CGGCCG CG[GA][CT]^CG BsiHKAI 640 GAGCTC G[AT]GC[AT]^C BsmBI 357 CGTCTC CGTCTC(1/5) BstAPI 312 GCACACCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 660 CATCC GGATG(2/0) BstYI 881 AGATCT [GA]^GATC[CT] CelII 642 GCTCAGC GC^T[ACGT]AGC CpoI 155 CGGACCG CG^G[AT]CCG DraIII 313 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 263 CGGCCG C^GGCCG EclXI 263 CGGCCG C^GGCCG Eco47III 560 AGCGCT AGC^GCT Eco52I 263 CGGCCG C^GGCCG EcoNI 1041 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 113 CCTTGG C^C[AT][AT]GG FokI 660 CATCC GGATG(9/13) FspI 242 TGCGCA TGC^GCA HgaI 230 GCGTC GACGC(5/10) Hin1I 118 GGCGCC G[GA]^CG[CT]C KasI 118 GGCGCC G^GCGCC MaeII 539 ACGT A^CGT MflI 881 AGATCT [GA]^GATC[CT] MseI 1111 TTAA T^TAA MslI 417 CACGCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 1034 GCCGGC GCC^GGC NarI 118 GGCGCC GG^CGCC NgoMIV 1034 GCCGGC G^CCGGC PflMI 465 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 585 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 76 CTGCAG CTGCA^G RsrII 155 CGGACCG CG^G[AT]CCG SacI 640 GAGCTC GAGCT^C SbfI 75 CCTGCAGG CCTGCA^GG SfcI 76 CTGCAG C^T[GA][CT]AG SfiI 396 GGCCTGTACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 118 GGCGCC GGC^GCC Sse8387I 75 CCTGCAGG CCTGCA^GG StyI 113 CCTTGG C^C[AT][AT]GG Tru9I 1111 TTAA T^TAA Van91I 465 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 881 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AfaI 333 GTAC GT^AC 401 GTAC AlwI 915 GATCC GGATC(4/5) 1133 GGATC ApaI 492 GGGCCC GGGCC^C 895 GGGCCC BpmI 61 CTCCAG CTGGAG(16/14) 472 CTGGAG BstEII 382 GGTGACC G^GT[ACGT]ACC 943 GGTGACC BstPI 382 GGTGACC G^GT[ACGT]ACC 943 GGTGACC EcoO65I 382 GGTGACC G^GT[ACGT]ACC 943 GGTGACC HaeII 118 GGCGCC [GA]GCGC^[CT] 560 AGCGCT KspI 159 CCGCGG CCGC^GG 956 CCGCGG PpuMI 268 GGGACCC [GA]G^G[AT]CC[CT] 583 GGGACCC PvuII 349 CAGCTG CAG^CTG 949 CAGCTG RsaI 333 GTAC GT^AC 401 GTAC SacII 159 CCGCGG CCGC^GG 956 CCGCGG TspRI 175 CAGTG CA[GC]TG[ACGT][ACGT]^ 975 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AccII AciI AluI AvaII BanI BanII BbvI BcnI BmyI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIV PleI Sau3AI Sau96I ScrFI TseI Tsp45I