################################## # NO CUT ENZYMES ( 155 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcsI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BseRI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1407I BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII CfoI Cfr10I CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeII MamI MflI MluI MspA1I MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccIII 911 TCCGGA T^CCGGA AcyI 754 GGCGTC G[GA]^CG[CT]C AflIII 641 ACATGT A^C[GA][CT]GT AocI 142 CCTGAGG CC^T[ACGT]AGG ApaI 773 GGGCCC GGGCC^C Asp700I 930 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 653 TGGCCA TGG^CCA BanII 773 GGGCCC G[GA]GC[CT]^C BclI 869 TGATCA T^GATCA BcnI 688 CCCGG CC^[GC]GG BfaI 986 CTAG C^TAG BmyI 773 GGGCCC G[AGT]GC[ACT]^C BpmI 463 CTCCAG CTGGAG(16/14) BsaHI 754 GGCGTC G[GA]^CG[CT]C BsaWI 911 TCCGGA [AT]^CCGG[AT] BseAI 911 TCCGGA T^CCGGA BsmFI 87 GTCCC GGGAC(10/14) BsmI 225 GCATTC GAATGC(1/-1) Bsp1286I 773 GGGCCC G[AGT]GC[ACT]^C BspDI 889 ATCGAT AT^CGAT BspEI 911 TCCGGA T^CCGGA BspLU11I 641 ACATGT A^CATGT BsrDI 74 GCAATG GCAATG(2/0) Bsu36I 142 CCTGAGG CC^T[ACGT]AGG ClaI 889 ATCGAT AT^CGAT DraII 773 GGGCCCC [GA]G^G[ACGT]CC[CT] DsaI 18 CCGTGG C^C[GA][CT]GG EaeI 653 TGGCCA [CT]^GGCC[GA] Eco81I 142 CCTGAGG CC^T[ACGT]AGG EcoO109I 773 GGGCCCC [GA]G^G[ACGT]CC[CT] FauI 492 GCGGG CCCGC(4/6) FbaI 869 TGATCA T^GATCA HgaI 755 GCGTC GACGC(5/10) Hin1I 754 GGCGTC G[GA]^CG[CT]C MaeI 986 CTAG C^TAG MfeI 425 CAATTG C^AATTG MluNI 653 TGGCCA TGG^CCA MroI 911 TCCGGA T^CCGGA MscI 653 TGGCCA TGG^CCA MunI 425 CAATTG C^AATTG MwoI 616 GCTCTCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 688 CCCGG CC^[GC]GG NspI 641 ACATGT [GA]CATG^[CT] PstI 249 CTGCAG CTGCA^G StuI 969 AGGCCT AGG^CCT TaqI 890 TCGA T^CGA TthHB8I 890 TCGA T^CGA XmnI 930 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### BanI 697 GGTGCC G^G[CT][GA]CC 712 GGTGCC BsgI 37 CTGCAC GTGCAG(16/14) 67 CTGCAC BsmAI 136 GTCTC GTCTC(1/5) 843 GTCTC BstNI 656 CCAGG CC^[AT]GG 963 CCTGG Cfr13I 773 GGGCC G^G[ACGT]CC 774 GGCCC EcoRII 656 CCAGG ^CC[AT]GG 963 CCTGG EcoT14I 90 CCTTGG C^C[AT][AT]GG 708 CCAAGG HapII 689 CCGG C^CGG 912 CCGG HpaII 689 CCGG C^CGG 912 CCGG MslI 70 CACAGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 591 CATCACCATG MspI 689 CCGG C^CGG 912 CCGG MvaI 656 CCAGG CC^[AT]GG 963 CCTGG Sau96I 773 GGGCC G^G[ACGT]CC 774 GGCCC SfcI 249 CTGCAG C^T[GA][CT]AG 387 CTGTAG SspI 358 AATATT AAT^ATT 795 AATATT StyI 90 CCTTGG C^C[AT][AT]GG 708 CCAAGG TfiI 219 GAATC G^A[AT]TC 819 GAATC Tsp45I 154 GTGAC ^GT[GC]AC 977 GTGAC Tsp509I 426 AATT ^AATT 880 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AciI AfaI AluI AlwI BbvI BsaJI BsiYI BslI BsrI BssKI BstF5I Cac8I DdeI DpnII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeIII MboI MboII MnlI MseI NdeII NlaIII NlaIV PleI RsaI Sau3AI ScrFI SfaNI Tru9I TseI TspRI