(Circular) (Six-base) MAPSORT of: pFN21AE9506.seq Check: 7245 from: 1 to: 5701 pFN21AE9506 5701 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3511 278 Size: 53 83 186 936 1975 2468 Fragments arranged by size: 2468 1975 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3044 1937 Size: 1107 4594 Acc65I G'GTAC_C Cuts at: 3022 3022 Size: 5701 AclI AA'CG_TT Cuts at: 1563 3828 4201 1563 Size: 2265 373 3063 Fragments arranged by size: 3063 2265 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3764 5148 5416 1632 Size: 2132 1384 268 1917 Fragments arranged by size: 2132 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5504 AflIII A'CryG_T Cuts at: 1203 2646 4600 5577 1203 Size: 1443 1954 977 1327 Fragments arranged by size: 1954 1443 1327 977 AgeI A'CCGG_T Cuts at: 4532 4532 Size: 5701 AhdI GACnn_n'nnGTC Cuts at: 4430 4430 Size: 5701 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5701 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2097 2431 1184 Size: 913 334 4454 Fragments arranged by size: 4454 913 334 AlwNI CAG_nnn'CTG Cuts at: 3444 5016 3444 Size: 1572 4129 ApaI G_GGCC'C Cuts at: 2782 2782 Size: 5701 ApaLI G'TGCA_C Cuts at: 3758 4914 3758 Size: 1156 4545 ApoI r'AATT_y Cuts at: 1493 3010 3236 3388 5497 1493 Size: 1517 226 152 2109 1697 Fragments arranged by size: 2109 1697 1517 226 152 AseI AT'TA_AT Cuts at: 160 1032 4255 160 Size: 872 3223 1606 Fragments arranged by size: 3223 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5701 AvaI C'yCGr_G Cuts at: 1870 1943 3026 1870 Size: 73 1083 4545 Fragments arranged by size: 4545 1083 73 BamHI G'GATC_C Cuts at: 3031 3398 3031 Size: 367 5334 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2702 2717 3022 4477 618 Size: 325 217 627 915 15 305 1455 1842 Fragments arranged by size: 1842 1455 915 627 325 305 217 15 BanII G_rGCy'C Cuts at: 1391 2782 3020 1391 Size: 1391 238 4072 Fragments arranged by size: 4072 1391 238 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4730 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3892 3926 1903 Size: 34 1955 34 3678 Fragments arranged by size: 3678 1955 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3593 4803 3593 Size: 1210 4491 BclI T'GATC_A Cuts at: 1548 2874 1548 Size: 1326 4375 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4312 136 Size: 107 122 71 631 605 2640 1525 Fragments arranged by size: 2640 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5696 5696 Size: 5701 BlpI GC'TnA_GC Cuts at: 3118 3118 Size: 5701 Bme1580I G_kGCm'C Cuts at: 1790 2782 3762 4918 1790 Size: 992 980 1156 2573 Fragments arranged by size: 2573 1156 992 980 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5701 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3432 4390 442 Size: 416 53 83 399 24 2015 958 1753 Fragments arranged by size: 2015 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5701 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2453 4361 1239 Size: 1214 1908 2579 Fragments arranged by size: 2579 1908 1214 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3826 4691 4989 3826 Size: 865 298 4538 Fragments arranged by size: 4538 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4364 882 Size: 617 2865 2219 Fragments arranged by size: 2865 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3492 493 Size: 711 2288 2702 Fragments arranged by size: 2702 2288 711 BsaBI GATnn'nnATC Cuts at: 5695 5695 Size: 5701 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2760 3508 3890 4525 Size: 53 83 186 936 1227 748 382 635 Cuts at: 4525 275 Size: 1451 Fragments arranged by size: 1451 1227 936 748 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2916 4134 4532 4806 4953 5364 2916 Size: 1218 398 274 147 411 3253 Fragments arranged by size: 3253 1218 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5476 Fragments arranged by size: 5476 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5701 BseYI C'CCAG_C Cuts at: 2775 4904 5400 2775 Size: 2129 496 3076 Fragments arranged by size: 3076 2129 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2027 2057 1163 Size: 573 291 30 4807 Fragments arranged by size: 4807 573 291 30 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3151 3912 4940 1540 Size: 275 186 1150 761 1028 2301 Fragments arranged by size: 2301 1150 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3762 3847 4918 3762 Size: 85 1071 4545 Fragments arranged by size: 4545 1071 85 BsmI GAATG_Cn' Cuts at: 2230 3212 3305 2230 Size: 982 93 4626 Fragments arranged by size: 4626 982 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2782 3020 3762 3847 4918 1391 Size: 399 992 238 742 85 1071 2174 Fragments arranged by size: 2174 1071 992 742 399 238 85 BspEI T'CCGG_A Cuts at: 2916 5364 2916 Size: 2448 3253 BspHI T'CATG_A Cuts at: 3590 5320 3590 Size: 1730 3971 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3056 844 Size: 2212 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3588 1394 Size: 429 1765 3507 Fragments arranged by size: 3507 1765 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2086 4196 4370 65 Size: 1183 838 2110 174 1396 Fragments arranged by size: 2110 1396 1183 838 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4345 4532 1700 Size: 110 2535 187 2869 Fragments arranged by size: 2869 2535 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5701 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5701 BssSI C'ACGA_G Cuts at: 3761 4773 3761 Size: 1012 4689 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5701 BstBI TT'CG_AA Cuts at: 4505 4505 Size: 5701 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5701 BstYI r'GATC_y Cuts at: 1972 3031 3398 3783 3800 5252 5338 5350 5696 Size: 1059 367 385 17 1452 86 12 346 Cuts at: 5696 1972 Size: 1977 Fragments arranged by size: 1977 1452 1059 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2148 2148 Size: 5701 BtgI C'CryG_G Cuts at: 513 1065 2004 2023 513 Size: 552 939 19 4191 Fragments arranged by size: 4191 939 552 19 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4962 Fragments arranged by size: 4962 683 56 BtsI GCAGTG_nn' Cuts at: 2442 3217 4011 4031 2442 Size: 775 794 20 4112 Fragments arranged by size: 4112 794 775 20 ClaI AT'CG_AT Cuts at: 1554 2895 1554 Size: 1341 4360 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2418 2453 529 Size: 35 1854 35 3777 Fragments arranged by size: 3777 1854 35 35 DraI TTT'AAA Cuts at: 3005 3005 Size: 5701 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4708 809 Size: 507 3392 1802 Fragments arranged by size: 3392 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2658 3148 4037 Size: 54 1425 50 275 18 828 490 889 Cuts at: 4037 5666 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 828 490 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3148 1537 Size: 275 1336 4090 Fragments arranged by size: 4090 1336 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3631 1353 Size: 2278 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2699 4288 4662 4808 387 Size: 1064 138 1110 1589 374 146 1280 Fragments arranged by size: 1589 1280 1110 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2453 3764 4361 5148 5416 1239 Size: 393 821 1311 597 787 268 1524 Fragments arranged by size: 1524 1311 821 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2779 2779 Size: 5701 EcoRI G'AATT_C Cuts at: 3010 3388 3010 Size: 378 5323 FspI TGC'GCA Cuts at: 4207 4207 Size: 5701 HaeII r_GCGC'y Cuts at: 1678 4848 1678 Size: 3170 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4342 4374 4416 4448 1373 Size: 32 135 32 2770 32 42 32 2626 Fragments arranged by size: 2770 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3045 3291 669 Size: 988 281 1107 246 3079 Fragments arranged by size: 3079 1107 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5701 HpaI GTT'AAC Cuts at: 3291 3291 Size: 5701 KpnI G_GTAC'C Cuts at: 3026 3026 Size: 5701 MfeI C'AATT_G Cuts at: 2430 3300 2430 Size: 870 4831 MluI A'CGCG_T Cuts at: 5577 5577 Size: 5701 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2163 4815 4999 1305 Size: 858 2652 184 2007 Fragments arranged by size: 2652 2007 858 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2660 10 Size: 54 1425 343 828 3051 Fragments arranged by size: 3051 1425 828 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2079 2600 3659 4018 4177 518 Size: 648 913 521 1059 359 159 2042 Fragments arranged by size: 2042 1059 913 648 521 359 159 MspA1I CmG'CkG Cuts at: 1606 3117 3794 4942 5187 1606 Size: 1511 677 1148 245 2120 Fragments arranged by size: 2120 1511 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4210 Fragments arranged by size: 4210 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5701 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5701 NotI GC'GGCC_GC Cuts at: 3148 3148 Size: 5701 NspI r_CATG'y Cuts at: 1132 1229 2650 3059 4604 1132 Size: 97 1421 409 1545 2229 Fragments arranged by size: 2229 1545 1421 409 97 PasI CC'CwG_GG Cuts at: 1348 2968 1348 Size: 1620 4081 PciI A'CATG_T Cuts at: 2646 4600 2646 Size: 1954 3747 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5701 PmeI GTTT'AAAC Cuts at: 3005 3005 Size: 5701 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3770 3802 5283 5315 3770 Size: 32 1481 32 4156 Fragments arranged by size: 4156 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5701 PsiI TTA'TAA Cuts at: 3271 3271 Size: 5701 PspOMI G'GGCC_C Cuts at: 2778 2778 Size: 5701 PstI C_TGCA'G Cuts at: 830 2258 3053 830 Size: 1428 795 3478 Fragments arranged by size: 3478 1428 795 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5701 SalI G'TCGA_C Cuts at: 1936 3043 1936 Size: 1107 4594 SbfI CC_TGCA'GG Cuts at: 3053 3053 Size: 5701 ScaI AGT'ACT Cuts at: 3949 5661 3949 Size: 1712 3989 SfcI C'TryA_G Cuts at: 826 1045 2254 2392 3049 4184 4865 5056 826 Size: 219 1209 138 657 1135 681 191 1471 Fragments arranged by size: 1471 1209 1135 681 657 219 191 138 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5701 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5701 SmaI CCC'GGG Cuts at: 1872 3028 1872 Size: 1156 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3805 4706 4968 820 Size: 197 926 1862 901 262 1553 Fragments arranged by size: 1862 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3492 493 Size: 2999 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5701 SphI G_CATG'C Cuts at: 1132 3059 1132 Size: 1927 3774 SspI AAT'ATT Cuts at: 5 52 2365 2802 3625 5 Size: 47 2313 437 823 2081 Fragments arranged by size: 2313 2081 823 437 47 StuI AGG'CCT Cuts at: 1517 2976 1517 Size: 1459 4242 StyI C'CwwG_G Cuts at: 513 1065 2004 2095 2713 3140 513 Size: 552 939 91 618 427 3074 Fragments arranged by size: 3074 939 618 552 427 91 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2033 2156 2251 3728 3745 3898 4083 Size: 1255 453 123 95 1477 17 153 185 Cuts at: 4083 4528 325 Size: 445 1498 Fragments arranged by size: 1498 1477 1255 453 445 185 153 123 95 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2241 2330 3947 Size: 274 80 33 51 1443 264 89 1617 Cuts at: 3947 5659 96 Size: 1712 138 Fragments arranged by size: 1712 1617 1443 274 264 138 89 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5661 5693 5661 Size: 32 5669 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5701 XbaI T'CTAG_A Cuts at: 3037 3037 Size: 5701 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5701 XmaI C'CCGG_G Cuts at: 1870 3026 1870 Size: 1156 4545 XmnI GAAnn'nnTTC Cuts at: 2939 3830 2939 Size: 891 4810 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3509 276 Size: 53 83 186 936 1975 2468 Fragments arranged by size: 2468 1975 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI