(Circular) (Six-base) MAPSORT of: pFN21AE4483.seq Check: 5439 from: 1 to: 6205 pFN21AE4483 6205 bp With 182 enzymes: SgfI * March 28, 2012 13:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4015 278 Size: 53 83 186 936 2479 2468 Fragments arranged by size: 2479 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3204 3548 1937 Size: 1267 344 4594 Fragments arranged by size: 4594 1267 344 Acc65I G'GTAC_C Cuts at: 3526 3526 Size: 6205 AclI AA'CG_TT Cuts at: 1563 4332 4705 1563 Size: 2769 373 3063 Fragments arranged by size: 3063 2769 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3055 4268 5652 5920 1632 Size: 1423 1213 1384 268 1917 Fragments arranged by size: 1917 1423 1384 1213 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6008 AflIII A'CryG_T Cuts at: 1203 2672 5104 6081 1203 Size: 1469 2432 977 1327 Fragments arranged by size: 2432 1469 1327 977 AgeI A'CCGG_T Cuts at: 5036 5036 Size: 6205 AhdI GACnn_n'nnGTC Cuts at: 4934 4934 Size: 6205 AleI CACnn'nnGTG Cuts at: 1166 2807 1166 Size: 1641 4564 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6205 AlwNI CAG_nnn'CTG Cuts at: 3148 3948 5520 3148 Size: 800 1572 3833 Fragments arranged by size: 3833 1572 800 ApaI G_GGCC'C Cuts at: 2595 2595 Size: 6205 ApaLI G'TGCA_C Cuts at: 2893 4262 5418 2893 Size: 1369 1156 3680 Fragments arranged by size: 3680 1369 1156 ApoI r'AATT_y Cuts at: 1493 3514 3740 3892 6001 1493 Size: 2021 226 152 2109 1697 Fragments arranged by size: 2109 2021 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4759 160 Size: 872 3727 1606 Fragments arranged by size: 3727 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6205 AvaI C'yCGr_G Cuts at: 1870 1943 2483 3018 3530 1870 Size: 73 540 535 512 4545 Fragments arranged by size: 4545 540 535 512 73 BamHI G'GATC_C Cuts at: 2581 3535 3902 2581 Size: 954 367 4884 Fragments arranged by size: 4884 954 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3526 4981 618 Size: 325 217 627 1739 1455 1842 Fragments arranged by size: 1842 1739 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2069 2595 3102 3438 3524 1391 Size: 678 526 507 336 86 4072 Fragments arranged by size: 4072 678 526 507 336 86 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2235 3061 3405 928 Size: 971 336 826 344 3728 Fragments arranged by size: 3728 971 826 344 336 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3164 3198 4396 4430 1903 Size: 34 1227 34 1198 34 3678 Fragments arranged by size: 3678 1227 1198 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4097 5307 4097 Size: 1210 4995 BclI T'GATC_A Cuts at: 1548 1548 Size: 6205 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4816 136 Size: 107 122 71 631 605 3144 1525 Fragments arranged by size: 3144 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6200 6200 Size: 6205 BlpI GC'TnA_GC Cuts at: 3622 3622 Size: 6205 Bme1580I G_kGCm'C Cuts at: 1790 2595 2897 4266 5422 1790 Size: 805 302 1369 1156 2573 Fragments arranged by size: 2573 1369 1156 805 302 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6205 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3936 4894 442 Size: 416 53 83 399 24 2519 958 1753 Fragments arranged by size: 2519 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6205 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2646 3277 2646 Size: 631 5574 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2056 3484 4865 1239 Size: 817 1428 1381 2579 Fragments arranged by size: 2579 1428 1381 817 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2707 4330 5195 5493 2707 Size: 1623 865 298 3419 Fragments arranged by size: 3419 1623 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3219 4868 882 Size: 617 1720 1649 2219 Fragments arranged by size: 2219 1720 1649 617 BsaAI yAC'GTr Cuts at: 493 1204 2524 3996 493 Size: 711 1320 1472 2702 Fragments arranged by size: 2702 1472 1320 711 BsaBI GATnn'nnATC Cuts at: 3059 6199 3059 Size: 3140 3065 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2349 4012 4394 5029 Size: 53 83 186 936 816 1663 382 635 Cuts at: 5029 275 Size: 1451 Fragments arranged by size: 1663 1451 936 816 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2632 4638 5036 5310 5457 5868 2632 Size: 2006 398 274 147 411 2969 Fragments arranged by size: 2969 2006 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2508 2538 3425 3455 1178 Size: 30 165 30 1105 30 887 30 3928 Fragments arranged by size: 3928 1105 887 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3227 1186 Size: 2041 4164 BseYI C'CCAG_C Cuts at: 3372 5408 5904 3372 Size: 2036 496 3673 Fragments arranged by size: 3673 2036 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2294 2315 1163 Size: 573 558 21 5053 Fragments arranged by size: 5053 573 558 21 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3655 4416 5444 1540 Size: 275 186 1654 761 1028 2301 Fragments arranged by size: 2301 1654 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2897 3438 4266 4351 5422 2897 Size: 541 828 85 1071 3680 Fragments arranged by size: 3680 1071 828 541 85 BsmI GAATG_Cn' Cuts at: 3187 3716 3809 3187 Size: 529 93 5583 Fragments arranged by size: 5583 529 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2069 2595 2897 3102 3438 3524 4266 Size: 399 279 526 302 205 336 86 742 Cuts at: 4266 4351 5422 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 526 399 336 302 279 205 86 85 BspEI T'CCGG_A Cuts at: 2632 5868 2632 Size: 3236 2969 BspHI T'CATG_A Cuts at: 4094 5824 4094 Size: 1730 4475 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3560 844 Size: 2716 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2289 3169 4092 1394 Size: 429 466 880 923 3507 Fragments arranged by size: 3507 923 880 466 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2436 4700 4874 65 Size: 1183 1188 2264 174 1396 Fragments arranged by size: 2264 1396 1188 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4849 5036 1700 Size: 110 3039 187 2869 Fragments arranged by size: 3039 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3392 96 Size: 3296 2909 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6205 BssSI C'ACGA_G Cuts at: 2318 4265 5277 2318 Size: 1947 1012 3246 Fragments arranged by size: 3246 1947 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6205 BstBI TT'CG_AA Cuts at: 5009 5009 Size: 6205 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6205 BstXI CCAn_nnnn'nTGG Cuts at: 2425 2425 Size: 6205 BstYI r'GATC_y Cuts at: 1972 2581 2922 3237 3535 3902 4287 4304 5756 Size: 609 341 315 298 367 385 17 1452 Cuts at: 5756 5842 5854 6200 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 609 385 367 346 341 315 298 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5653 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5466 Fragments arranged by size: 5466 683 56 BtsI GCAGTG_nn' Cuts at: 3721 4515 4535 3721 Size: 794 20 5391 Fragments arranged by size: 5391 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6205 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6170 DraI TTT'AAA Cuts at: 3509 3509 Size: 6205 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5212 809 Size: 507 3896 1802 Fragments arranged by size: 3896 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2209 2526 3652 Size: 54 1425 50 275 18 379 317 1126 Cuts at: 3652 4541 6170 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1126 889 379 317 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3652 1537 Size: 275 1840 4090 Fragments arranged by size: 4090 1840 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4135 1353 Size: 2782 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4792 5166 5312 387 Size: 1064 138 3203 374 146 1280 Fragments arranged by size: 3203 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3436 3436 Size: 6205 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2056 3055 3484 4268 4865 5652 5920 Size: 393 424 999 429 784 597 787 268 Cuts at: 5920 1239 Size: 1524 Fragments arranged by size: 1524 999 787 784 597 429 424 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3284 3284 Size: 6205 EcoO109I rG'GnC_Cy Cuts at: 2176 3258 2176 Size: 1082 5123 EcoRI G'AATT_C Cuts at: 3514 3892 3514 Size: 378 5827 FspI TGC'GCA Cuts at: 4711 4711 Size: 6205 HaeII r_GCGC'y Cuts at: 1678 5352 1678 Size: 3674 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2184 2216 4846 4878 4920 Size: 32 135 32 612 32 2630 32 42 Cuts at: 4920 4952 1373 Size: 32 2626 Fragments arranged by size: 2630 2626 612 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2368 2851 3549 3795 669 Size: 988 281 430 483 698 246 3079 Fragments arranged by size: 3079 988 698 483 430 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6205 HpaI GTT'AAC Cuts at: 3795 3795 Size: 6205 KpnI G_GTAC'C Cuts at: 3530 3530 Size: 6205 MfeI C'AATT_G Cuts at: 2784 3804 2784 Size: 1020 5185 MluI A'CGCG_T Cuts at: 2672 6081 2672 Size: 3409 2796 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5319 5503 1305 Size: 4014 184 2007 Fragments arranged by size: 4014 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4383 Fragments arranged by size: 4383 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2807 3353 4163 4522 4681 518 Size: 648 1641 546 810 359 159 2042 Fragments arranged by size: 2042 1641 810 648 546 359 159 MspA1I CmG'CkG Cuts at: 1606 3376 3621 4298 5446 5691 1606 Size: 1770 245 677 1148 245 2120 Fragments arranged by size: 2120 1770 1148 677 245 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5653 NdeI CA'TA_TG Cuts at: 387 2791 387 Size: 2404 3801 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6205 NotI GC'GGCC_GC Cuts at: 3652 3652 Size: 6205 NspI r_CATG'y Cuts at: 1132 1229 3031 3563 5108 1132 Size: 97 1802 532 1545 2229 Fragments arranged by size: 2229 1802 1545 532 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6205 PciI A'CATG_T Cuts at: 5104 5104 Size: 6205 PflMI CCAn_nnn'nTGG Cuts at: 1108 2759 1108 Size: 1651 4554 PmeI GTTT'AAAC Cuts at: 3509 3509 Size: 6205 PmlI CAC'GTG Cuts at: 2524 2524 Size: 6205 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4274 4306 5787 5819 4274 Size: 32 1481 32 4660 Fragments arranged by size: 4660 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2176 2176 Size: 6205 PshAI GACnn'nnGTC Cuts at: 1613 2135 1613 Size: 522 5683 PsiI TTA'TAA Cuts at: 3775 3775 Size: 6205 PspOMI G'GGCC_C Cuts at: 2591 2591 Size: 6205 PstI C_TGCA'G Cuts at: 830 2237 2463 3557 830 Size: 1407 226 1094 3478 Fragments arranged by size: 3478 1407 1094 226 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6205 SacI G_AGCT'C Cuts at: 3438 3438 Size: 6205 SalI G'TCGA_C Cuts at: 1936 3547 1936 Size: 1611 4594 SbfI CC_TGCA'GG Cuts at: 3557 3557 Size: 6205 ScaI AGT'ACT Cuts at: 4453 6165 4453 Size: 1712 4493 SfcI C'TryA_G Cuts at: 826 1045 2233 2459 2506 3553 4688 5369 5560 Size: 219 1188 226 47 1047 1135 681 191 Cuts at: 5560 826 Size: 1471 Fragments arranged by size: 1471 1188 1135 1047 681 226 219 191 47 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6205 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6205 SmaI CCC'GGG Cuts at: 1872 3020 3532 1872 Size: 1148 512 4545 Fragments arranged by size: 4545 1148 512 SmlI C'TyrA_G Cuts at: 820 1017 1943 2686 4309 5210 5472 820 Size: 197 926 743 1623 901 262 1553 Fragments arranged by size: 1623 1553 926 901 743 262 197 SnaBI TAC'GTA Cuts at: 493 3996 493 Size: 3503 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6205 SphI G_CATG'C Cuts at: 1132 3031 3563 1132 Size: 1899 532 3774 Fragments arranged by size: 3774 1899 532 SspI AAT'ATT Cuts at: 5 52 4129 5 Size: 47 4077 2081 Fragments arranged by size: 4077 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6205 StyI C'CwwG_G Cuts at: 513 1065 2172 3644 513 Size: 552 1107 1472 3074 Fragments arranged by size: 3074 1472 1107 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4232 4249 4402 4587 5032 325 Size: 1255 2652 17 153 185 445 1498 Fragments arranged by size: 2652 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3392 4451 6163 Size: 274 80 33 51 1443 1415 1059 1712 Cuts at: 6163 96 Size: 138 Fragments arranged by size: 1712 1443 1415 1059 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2508 2540 6165 6197 2508 Size: 32 3625 32 2516 Fragments arranged by size: 3625 2516 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6205 XbaI T'CTAG_A Cuts at: 3541 3541 Size: 6205 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2359 2756 3196 3490 2359 Size: 397 440 294 5074 Fragments arranged by size: 5074 440 397 294 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6205 XmaI C'CCGG_G Cuts at: 1870 3018 3530 1870 Size: 1148 512 4545 Fragments arranged by size: 4545 1148 512 XmnI GAAnn'nnTTC Cuts at: 4334 4334 Size: 6205 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4013 276 Size: 53 83 186 936 2479 2468 Fragments arranged by size: 2479 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI