################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspHI AspI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmFI BspDI BspEI BsrBI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MspA1I MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspI StuI StyI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AcsI 3 GAATTC [GA]^AATT[CT] AcyI 322 GACGCC G[GA]^CG[CT]C AflIII 620 ACATGT A^C[GA][CT]GT AhdI 68 GACCCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 264 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 752 GGGCCC GGGCC^C ApoI 3 GAATTC [GA]^AATT[CT] AspEI 68 GACCCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 667 CCCGGG C^[CT]CG[GA]G BalI 401 TGGCCA TGG^CCA BanII 752 GGGCCC G[GA]GC[CT]^C BfaI 310 CTAG C^TAG BmyI 752 GGGCCC G[AGT]GC[ACT]^C BsaHI 322 GACGCC G[GA]^CG[CT]C BsaI 274 GAGACC GGTCTC(1/5) BsmI 42 GAATGC GAATGC(1/-1) BsoBI 667 CCCGGG C^[CT]CG[GA]G Bsp1286I 752 GGGCCC G[AGT]GC[ACT]^C Bsp1407I 611 TGTACA T^GTACA BspHI 458 TCATGA T^CATGA BspLU11I 620 ACATGT A^CATGT BsrFI 490 GCCGGC [GA]^CCGG[CT] BsrGI 611 TGTACA T^GTACA BstAPI 509 GCACGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 711 GGTCACC G^GT[ACGT]ACC BstPI 711 GGTCACC G^GT[ACGT]ACC BstYI 30 GGATCT [GA]^GATC[CT] Cfr10I 490 GCCGGC [GA]^CCGG[CT] EaeI 401 TGGCCA [CT]^GGCC[GA] Eam1105I 68 GACCCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 711 GGTCACC G^GT[ACGT]ACC EcoRI 3 GAATTC G^AATTC HaeII 470 GGCGCT [GA]GCGC^[CT] HgaI 322 GACGC GACGC(5/10) Hin1I 322 GACGCC G[GA]^CG[CT]C MaeI 310 CTAG C^TAG MflI 30 GGATCT [GA]^GATC[CT] MluNI 401 TGGCCA TGG^CCA MscI 401 TGGCCA TGG^CCA NaeI 490 GCCGGC GCC^GGC NgoMIV 490 GCCGGC G^CCGGC PflMI 263 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 66 AGGACCC [GA]G^G[AT]CC[CT] PshAI 639 GACTCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 954 CTGCAG CTGCA^G RcaI 458 TCATGA T^CATGA SfcI 954 CTGCAG C^T[GA][CT]AG SmaI 667 CCCGGG CCC^GGG SphI 698 GCATGC GCATG^C SspBI 611 TGTACA T^GTACA TfiI 544 GAATC G^A[AT]TC Van91I 263 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 30 GGATCT [GA]^GATC[CT] XmaCI 667 CCCGGG C^CCGGG XmaI 667 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### BcnI 667 CCCGG CC^[GC]GG 668 CCGGG BspMI 17 ACCTGC ACCTGC(4/8) 197 ACCTGC BsrDI 111 CATTGC GCAATG(2/0) 405 CATTGC BsrI 399 ACTGG ACTGG(1/-1) 263 CCAGT BstXI 404 CCATTGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 722 CCATGCTGCTGG DraII 66 AGGACCC [GA]G^G[ACGT]CC[CT] 752 GGGCCCC DraIII 92 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG 307 CACCTAGTG DsaI 374 CCGTGG C^C[GA][CT]GG 936 CCGTGG EcoO109I 66 AGGACCC [GA]G^G[ACGT]CC[CT] 752 GGGCCCC HapII 491 CCGG C^CGG 668 CCGG HpaII 491 CCGG C^CGG 668 CCGG MseI 778 TTAA T^TAA 917 TTAA MspI 491 CCGG C^CGG 668 CCGG NciI 667 CCCGG CC^[GC]GG 668 CCGGG PleI 355 GACTC GAGTC(4/5) 639 GACTC Tru9I 778 TTAA T^TAA 917 TTAA Tsp509I 4 AATT ^AATT 859 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AvaII BbsI BbvI BpuAI BsaJI BsgI BsiYI BslI BsmAI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI MaeII MaeIII MboI MboII MnlI MslI MvaI MvnI MwoI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScrFI SfaNI TseI Tsp45I TspRI