################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## Acc65I AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbsI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstZ17I Bsu36I CelII ClaI CpoI DraI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MboII MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tsp509I Tth111I TthHB8I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AatII 549 GACGTC GACGT^C AccI 442 GTCTAC GT^[AC][GT]AC AcyI 549 GACGTC G[GA]^CG[CT]C AlwNI 73 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG BalI 353 TGGCCA TGG^CCA BanI 460 GGTGCC G^G[CT][GA]CC BbrPI 640 CACGTG CAC^GTG BbvCI 628 GCTGAGG CCTCAGC(-5/-2) BfaI 493 CTAG C^TAG BsaAI 640 CACGTG [CT]AC^GT[GA] BsaHI 549 GACGTC G[GA]^CG[CT]C BsgI 79 CTGCAC GTGCAG(16/14) BsmAI 400 GTCTC GTCTC(1/5) BsmFI 379 GTCCC GGGAC(10/14) BsmI 522 GAATGC GAATGC(1/-1) BsrBI 202 GAGCGG CCGCTC(-3/-3) BsrFI 705 GCCGGT [GA]^CCGG[CT] BssHII 92 GCGCGC G^CGCGC BstXI 479 CCAAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 705 GCCGGT [GA]^CCGG[CT] DraIII 640 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 133 GACAGCCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 353 TGGCCA [CT]^GGCC[GA] HgaI 440 GCGTC GACGC(5/10) Hin1I 549 GACGTC G[GA]^CG[CT]C MaeI 493 CTAG C^TAG MluNI 353 TGGCCA TGG^CCA MscI 353 TGGCCA TGG^CCA MseI 364 TTAA T^TAA NcoI 554 CCATGG C^CATGG NspI 470 ACATGC [GA]CATG^[CT] PmaCI 640 CACGTG CAC^GTG PmlI 640 CACGTG CAC^GTG PstI 265 CTGCAG CTGCA^G SacI 496 GAGCTC GAGCT^C SfcI 265 CTGCAG C^T[GA][CT]AG StuI 293 AGGCCT AGG^CCT TfiI 608 GAATC G^A[AT]TC Tru9I 364 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AluI 5 AGCT AG^CT 497 AGCT ApaI 222 GGGCCC GGGCC^C 502 GGGCCC BspMI 233 ACCTGC ACCTGC(4/8) 320 ACCTGC BsrI 188 ACTGG ACTGG(1/-1) 408 CCAGT BstEII 33 GGTCACC G^GT[ACGT]ACC 282 GGTCACC BstF5I 333 CATCC GGATG(2/0) 654 CATCC BstPI 33 GGTCACC G^GT[ACGT]ACC 282 GGTCACC DraII 222 GGGCCCC [GA]G^G[ACGT]CC[CT] 502 GGGCCCC EcoO109I 222 GGGCCCC [GA]G^G[ACGT]CC[CT] 502 GGGCCCC EcoO65I 33 GGTCACC G^GT[ACGT]ACC 282 GGTCACC FokI 333 CATCC GGATG(9/13) 654 CATCC HaeII 75 GGCGCT [GA]GCGC^[CT] 207 GGCGCT MaeII 550 ACGT A^CGT 641 ACGT MslI 333 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 537 CACAGTGGTG MspA1I 251 CCGCTG C[AC]G^C[GT]G 418 CAGCGG PleI 117 GACTC GAGTC(4/5) 377 GAGTC SmaI 153 CCCGGG CCC^GGG 371 CCCGGG TspRI 409 CAGTG CA[GC]TG[ACGT][ACGT]^ 539 CAGTG XcmI 455 CCAAGGGTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 698 CCACTTTGCCGGTGG XmaCI 153 CCCGGG C^CCGGG 371 CCCGGG XmaI 153 CCCGGG C^CCGGG 371 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AccII AciI AlwI AspHI AvaI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsoBI Bsp1286I BssKI BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EcoRII EcoT14I FauI Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MflI MnlI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI StyI TseI Tsp45I XhoII