(Circular) (Six-base) MAPSORT of: pFN21AE6654.seq Check: 8339 from: 1 to: 5437 pFN21AE6654 5437 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2558 3247 278 Size: 53 83 186 936 1022 689 2468 Fragments arranged by size: 2468 1022 936 689 186 83 53 AccI GT'mk_AC Cuts at: 1937 2448 2780 1937 Size: 511 332 4594 Fragments arranged by size: 4594 511 332 Acc65I G'GTAC_C Cuts at: 2758 2758 Size: 5437 AclI AA'CG_TT Cuts at: 1563 3564 3937 1563 Size: 2001 373 3063 Fragments arranged by size: 3063 2001 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3500 4884 5152 1632 Size: 1868 1384 268 1917 Fragments arranged by size: 1917 1868 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5240 AflIII A'CryG_T Cuts at: 1203 4336 5313 1203 Size: 3133 977 1327 Fragments arranged by size: 3133 1327 977 AgeI A'CCGG_T Cuts at: 4268 4268 Size: 5437 AhdI GACnn_n'nnGTC Cuts at: 4166 4166 Size: 5437 AleI CACnn'nnGTG Cuts at: 1166 2546 1166 Size: 1380 4057 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5437 AlwNI CAG_nnn'CTG Cuts at: 2083 3180 4752 2083 Size: 1097 1572 2768 Fragments arranged by size: 2768 1572 1097 ApaI G_GGCC'C Cuts at: 2231 2511 2231 Size: 280 5157 ApaLI G'TGCA_C Cuts at: 3494 4650 3494 Size: 1156 4281 ApoI r'AATT_y Cuts at: 1493 2746 2972 3124 5233 1493 Size: 1253 226 152 2109 1697 Fragments arranged by size: 2109 1697 1253 226 152 AseI AT'TA_AT Cuts at: 160 1032 3991 160 Size: 872 2959 1606 Fragments arranged by size: 2959 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5437 AvaI C'yCGr_G Cuts at: 1870 1943 2158 2376 2504 2762 1870 Size: 73 215 218 128 258 4545 Fragments arranged by size: 4545 258 218 215 128 73 BamHI G'GATC_C Cuts at: 2767 3134 2767 Size: 367 5070 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2465 2758 4213 618 Size: 325 217 627 678 293 1455 1842 Fragments arranged by size: 1842 1455 678 627 325 293 217 BanII G_rGCy'C Cuts at: 1391 2231 2505 2511 2756 1391 Size: 840 274 6 245 4072 Fragments arranged by size: 4072 840 274 245 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4466 BbvCI CC'TCA_GC Cuts at: 2634 2634 Size: 5437 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3628 3662 1903 Size: 34 1691 34 3678 Fragments arranged by size: 3678 1691 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3329 4539 3329 Size: 1210 4227 BclI T'GATC_A Cuts at: 1548 1548 Size: 5437 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4048 136 Size: 107 122 71 631 605 2376 1525 Fragments arranged by size: 2376 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5432 5432 Size: 5437 BlpI GC'TnA_GC Cuts at: 2854 2854 Size: 5437 Bme1580I G_kGCm'C Cuts at: 1790 2159 2231 2470 2511 3498 4654 1790 Size: 369 72 239 41 987 1156 2573 Fragments arranged by size: 2573 1156 987 369 239 72 41 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5437 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2203 3168 4126 Size: 416 53 83 399 24 786 965 958 Cuts at: 4126 442 Size: 1753 Fragments arranged by size: 1753 965 958 786 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5437 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2139 2139 Size: 5437 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2303 2392 2596 4097 1239 Size: 1064 89 204 1501 2579 Fragments arranged by size: 2579 1501 1064 204 89 Bpu10I CC'TnA_GC Cuts at: 2634 2634 Size: 5437 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3562 4427 4725 3562 Size: 865 298 4274 Fragments arranged by size: 4274 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4100 882 Size: 617 2601 2219 Fragments arranged by size: 2601 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2647 3228 493 Size: 711 1443 581 2702 Fragments arranged by size: 2702 1443 711 581 BsaBI GATnn'nnATC Cuts at: 5431 5431 Size: 5437 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2555 3244 3626 4261 Size: 53 83 186 936 1022 689 382 635 Cuts at: 4261 275 Size: 1451 Fragments arranged by size: 1451 1022 936 689 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3870 4268 4542 4689 5100 3870 Size: 398 274 147 411 4207 Fragments arranged by size: 4207 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5212 Fragments arranged by size: 5212 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5437 BseYI C'CCAG_C Cuts at: 2215 2421 4640 5136 2215 Size: 206 2219 496 2516 Fragments arranged by size: 2516 2219 496 206 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2069 1163 Size: 573 333 4531 Fragments arranged by size: 4531 573 333 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2887 3648 4676 1540 Size: 275 186 886 761 1028 2301 Fragments arranged by size: 2301 1028 886 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2420 2505 2646 3498 3583 4654 2420 Size: 85 141 852 85 1071 3203 Fragments arranged by size: 3203 1071 852 141 85 85 BsmI GAATG_Cn' Cuts at: 2533 2948 3041 2533 Size: 415 93 4929 Fragments arranged by size: 4929 415 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2159 2231 2420 2470 2505 2511 2646 Size: 399 369 72 189 50 35 6 135 Cuts at: 2646 2756 3498 3583 4654 1391 Size: 110 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 369 189 135 110 85 72 50 35 6 BspEI T'CCGG_A Cuts at: 5100 5100 Size: 5437 BspHI T'CATG_A Cuts at: 3326 5056 3326 Size: 1730 3707 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2247 2334 2792 844 Size: 1403 87 458 3489 Fragments arranged by size: 3489 1403 458 87 BsrBI CCG'CTC Cuts at: 1394 1823 2209 3324 1394 Size: 429 386 1115 3507 Fragments arranged by size: 3507 1115 429 386 BsrDI GCAATG_nn' Cuts at: 65 1248 3932 4106 65 Size: 1183 2684 174 1396 Fragments arranged by size: 2684 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2710 4081 4268 1700 Size: 110 900 1371 187 2869 Fragments arranged by size: 2869 1371 900 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5437 BssHII G'CGCG_C Cuts at: 1925 2097 1925 Size: 172 5265 BssSI C'ACGA_G Cuts at: 3497 4509 3497 Size: 1012 4425 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5437 BstBI TT'CG_AA Cuts at: 4241 4241 Size: 5437 BstEII G'GTnAC_C Cuts at: 1184 2038 2287 1184 Size: 854 249 4334 Fragments arranged by size: 4334 854 249 BstXI CCAn_nnnn'nTGG Cuts at: 2491 2491 Size: 5437 BstYI r'GATC_y Cuts at: 1972 2131 2309 2517 2767 3134 3519 3536 4988 Size: 159 178 208 250 367 385 17 1452 Cuts at: 4988 5074 5086 5432 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 250 208 178 159 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2232 2559 2694 513 Size: 552 939 228 327 135 3256 Fragments arranged by size: 3256 939 552 327 228 135 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4698 Fragments arranged by size: 4698 683 56 BtsI GCAGTG_nn' Cuts at: 2953 3747 3767 2953 Size: 794 20 4623 Fragments arranged by size: 4623 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5437 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5402 DraI TTT'AAA Cuts at: 2741 2741 Size: 5437 DraIII CAC_nnn'GTG Cuts at: 2650 2650 Size: 5437 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2144 4444 809 Size: 507 828 2300 1802 Fragments arranged by size: 2300 1802 828 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2358 2884 3773 Size: 54 1425 50 275 18 528 526 889 Cuts at: 3773 5402 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 528 526 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2884 1537 Size: 275 1072 4090 Fragments arranged by size: 4090 1072 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3367 1353 Size: 2014 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2144 4024 4398 4544 387 Size: 1064 138 555 1880 374 146 1280 Fragments arranged by size: 1880 1280 1064 555 374 146 138 EcoICRI GAG'CTC Cuts at: 2503 2503 Size: 5437 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2303 2392 2596 3500 4097 4884 5152 Size: 393 671 89 204 904 597 787 268 Cuts at: 5152 1239 Size: 1524 Fragments arranged by size: 1524 904 787 671 597 393 268 204 89 EcoO109I rG'GnC_Cy Cuts at: 2228 2508 2228 Size: 280 5157 EcoRI G'AATT_C Cuts at: 2746 3124 2746 Size: 378 5059 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2317 2317 Size: 5437 FspI TGC'GCA Cuts at: 3943 3943 Size: 5437 HaeII r_GCGC'y Cuts at: 1678 2084 2216 4584 1678 Size: 406 132 2368 2531 Fragments arranged by size: 2531 2368 406 132 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4078 4110 4152 4184 1373 Size: 32 135 32 2506 32 42 32 2626 Fragments arranged by size: 2626 2506 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2781 3027 669 Size: 988 281 843 246 3079 Fragments arranged by size: 3079 988 843 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5437 HpaI GTT'AAC Cuts at: 3027 3027 Size: 5437 KpnI G_GTAC'C Cuts at: 2762 2762 Size: 5437 MfeI C'AATT_G Cuts at: 3036 3036 Size: 5437 MluI A'CGCG_T Cuts at: 5313 5313 Size: 5437 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4551 4735 1305 Size: 3246 184 2007 Fragments arranged by size: 3246 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2360 10 Size: 54 1425 343 528 3087 Fragments arranged by size: 3087 1425 528 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2342 2546 3395 3754 3913 518 Size: 648 1176 204 849 359 159 2042 Fragments arranged by size: 2042 1176 849 648 359 204 159 MspA1I CmG'CkG Cuts at: 1606 2258 2425 2853 3530 4678 4923 1606 Size: 652 167 428 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 652 428 245 167 NcoI C'CATG_G Cuts at: 513 1065 2004 2559 513 Size: 552 939 555 3391 Fragments arranged by size: 3391 939 555 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5437 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5437 NotI GC'GGCC_GC Cuts at: 2884 2884 Size: 5437 NspI r_CATG'y Cuts at: 1132 1229 2479 2795 4340 1132 Size: 97 1250 316 1545 2229 Fragments arranged by size: 2229 1545 1250 316 97 PasI CC'CwG_GG Cuts at: 1348 2387 1348 Size: 1039 4398 PciI A'CATG_T Cuts at: 4336 4336 Size: 5437 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5437 PfoI T'CCnGG_A Cuts at: 2280 2280 Size: 5437 PmeI GTTT'AAAC Cuts at: 2741 2741 Size: 5437 PmlI CAC'GTG Cuts at: 2647 2647 Size: 5437 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3506 3538 5019 5051 3506 Size: 32 1481 32 3892 Fragments arranged by size: 3892 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5437 PsiI TTA'TAA Cuts at: 3007 3007 Size: 5437 PspOMI G'GGCC_C Cuts at: 2227 2507 2227 Size: 280 5157 PstI C_TGCA'G Cuts at: 830 2274 2789 830 Size: 1444 515 3478 Fragments arranged by size: 3478 1444 515 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5437 SacI G_AGCT'C Cuts at: 2505 2505 Size: 5437 SalI G'TCGA_C Cuts at: 1936 2779 1936 Size: 843 4594 SbfI CC_TGCA'GG Cuts at: 2789 2789 Size: 5437 ScaI AGT'ACT Cuts at: 3685 5397 3685 Size: 1712 3725 SfcI C'TryA_G Cuts at: 826 1045 2270 2785 3920 4601 4792 826 Size: 219 1225 515 1135 681 191 1471 Fragments arranged by size: 1471 1225 1135 681 515 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5437 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5437 SmaI CCC'GGG Cuts at: 1872 2160 2378 2764 1872 Size: 288 218 386 4545 Fragments arranged by size: 4545 386 288 218 SmlI C'TyrA_G Cuts at: 820 1017 1943 3541 4442 4704 820 Size: 197 926 1598 901 262 1553 Fragments arranged by size: 1598 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3228 493 Size: 2735 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5437 SphI G_CATG'C Cuts at: 1132 2795 1132 Size: 1663 3774 SspI AAT'ATT Cuts at: 5 52 3361 5 Size: 47 3309 2081 Fragments arranged by size: 3309 2081 47 StuI AGG'CCT Cuts at: 1517 2300 1517 Size: 783 4654 StyI C'CwwG_G Cuts at: 513 1065 2004 2292 2460 2484 2559 2876 513 Size: 552 939 288 168 24 75 317 3074 Fragments arranged by size: 3074 939 552 317 288 168 75 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2177 2694 3464 3481 3634 3819 4264 Size: 1255 597 517 770 17 153 185 445 Cuts at: 4264 325 Size: 1498 Fragments arranged by size: 1498 1255 770 597 517 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3683 5395 96 Size: 274 80 33 51 1443 1706 1712 138 Fragments arranged by size: 1712 1706 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5397 5429 5397 Size: 32 5405 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5437 XbaI T'CTAG_A Cuts at: 2773 2773 Size: 5437 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2467 2710 2467 Size: 243 5194 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5437 XmaI C'CCGG_G Cuts at: 1870 2158 2376 2762 1870 Size: 288 218 386 4545 Fragments arranged by size: 4545 386 288 218 XmnI GAAnn'nnTTC Cuts at: 3566 3566 Size: 5437 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2556 3245 276 Size: 53 83 186 936 1022 689 2468 Fragments arranged by size: 2468 1022 936 689 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI