(Linear) (Six-base) MAPSORT of: KIEE6654.seq Check: 3330 from: 1 to: 732 KIEE6654.orf With 182 enzymes: SgfI * March 16, 2015 11:35 .. AatII G_ACGT'C Cuts at: 0 553 732 Size: 553 179 AccI GT'mk_AC Cuts at: 0 443 732 Size: 443 289 AleI CACnn'nnGTG Cuts at: 0 541 732 Size: 541 191 AlwNI CAG_nnn'CTG Cuts at: 0 78 732 Size: 78 654 ApaI G_GGCC'C Cuts at: 0 226 506 732 Size: 226 280 226 Fragments arranged by size: 280 226 226 AvaI C'yCGr_G Cuts at: 0 153 371 499 732 Size: 153 218 128 233 Fragments arranged by size: 233 218 153 128 BanI G'GyrC_C Cuts at: 0 460 732 Size: 460 272 BanII G_rGCy'C Cuts at: 0 226 500 506 732 Size: 226 274 6 226 Fragments arranged by size: 274 226 226 6 BbvCI CC'TCA_GC Cuts at: 0 629 732 Size: 629 103 Bme1580I G_kGCm'C Cuts at: 0 154 226 465 506 732 Size: 154 72 239 41 226 Fragments arranged by size: 239 226 154 72 41 BmrI ACTGGGnnnn_n' Cuts at: 0 198 732 Size: 198 534 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 134 732 Size: 134 598 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 298 387 591 732 Size: 298 89 204 141 Fragments arranged by size: 298 204 141 89 Bpu10I CC'TnA_GC Cuts at: 0 629 732 Size: 629 103 BsaAI yAC'GTr Cuts at: 0 642 732 Size: 642 90 BsaHI Gr'CG_yC Cuts at: 0 550 732 Size: 550 182 BseYI C'CCAG_C Cuts at: 0 210 416 732 Size: 210 206 316 Fragments arranged by size: 316 210 206 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 64 732 Size: 64 668 BsiHKAI G_wGCw'C Cuts at: 0 415 500 641 732 Size: 415 85 141 91 Fragments arranged by size: 415 141 91 85 BsmI GAATG_Cn' Cuts at: 0 528 732 Size: 528 204 Bsp1286I G_dGCh'C Cuts at: 0 154 226 415 465 500 506 641 732 Size: 154 72 189 50 35 6 135 91 Fragments arranged by size: 189 154 135 91 72 50 35 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 242 329 732 Size: 242 87 403 Fragments arranged by size: 403 242 87 BsrBI CCG'CTC Cuts at: 0 204 732 Size: 204 528 BsrFI r'CCGG_y Cuts at: 0 705 732 Size: 705 27 BssHII G'CGCG_C Cuts at: 0 92 732 Size: 92 640 BstEII G'GTnAC_C Cuts at: 0 33 282 732 Size: 33 249 450 Fragments arranged by size: 450 249 33 BstXI CCAn_nnnn'nTGG Cuts at: 0 486 732 Size: 486 246 BstYI r'GATC_y Cuts at: 0 126 304 512 732 Size: 126 178 208 220 Fragments arranged by size: 220 208 178 126 BtgI C'CryG_G Cuts at: 0 227 554 689 732 Size: 227 327 135 43 Fragments arranged by size: 327 227 135 43 DraIII CAC_nnn'GTG Cuts at: 0 645 732 Size: 645 87 DrdI GACnn_nn'nnGTC Cuts at: 0 139 732 Size: 139 593 EaeI y'GGCC_r Cuts at: 0 353 732 Size: 353 379 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 139 732 Size: 139 593 EcoICRI GAG'CTC Cuts at: 0 498 732 Size: 498 234 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 298 387 591 732 Size: 298 89 204 141 Fragments arranged by size: 298 204 141 89 EcoO109I rG'GnC_Cy Cuts at: 0 223 503 732 Size: 223 280 229 Fragments arranged by size: 280 229 223 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 312 732 Size: 312 420 HaeII r_GCGC'y Cuts at: 0 79 211 732 Size: 79 132 521 Fragments arranged by size: 521 132 79 MscI TGG'CCA Cuts at: 0 355 732 Size: 355 377 MslI CAynn'nnrTG Cuts at: 0 337 541 732 Size: 337 204 191 Fragments arranged by size: 337 204 191 MspA1I CmG'CkG Cuts at: 0 253 420 732 Size: 253 167 312 Fragments arranged by size: 312 253 167 NcoI C'CATG_G Cuts at: 0 554 732 Size: 554 178 NspI r_CATG'y Cuts at: 0 474 732 Size: 474 258 PasI CC'CwG_GG Cuts at: 0 382 732 Size: 382 350 PfoI T'CCnGG_A Cuts at: 0 275 732 Size: 275 457 PmlI CAC'GTG Cuts at: 0 642 732 Size: 642 90 PspOMI G'GGCC_C Cuts at: 0 222 502 732 Size: 222 280 230 Fragments arranged by size: 280 230 222 PstI C_TGCA'G Cuts at: 0 269 732 Size: 269 463 SacI G_AGCT'C Cuts at: 0 500 732 Size: 500 232 SfcI C'TryA_G Cuts at: 0 265 732 Size: 265 467 SmaI CCC'GGG Cuts at: 0 155 373 732 Size: 155 218 359 Fragments arranged by size: 359 218 155 StuI AGG'CCT Cuts at: 0 295 732 Size: 295 437 StyI C'CwwG_G Cuts at: 0 287 455 479 554 732 Size: 287 168 24 75 178 Fragments arranged by size: 287 178 168 75 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 172 689 732 Size: 172 517 43 Fragments arranged by size: 517 172 43 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 462 705 732 Size: 462 243 27 Fragments arranged by size: 462 243 27 XmaI C'CCGG_G Cuts at: 0 153 371 732 Size: 153 218 361 Fragments arranged by size: 361 218 153 ZraI GAC'GTC Cuts at: 0 551 732 Size: 551 181 Enzymes that do cut: AatII AccI AleI AlwNI ApaI AvaI BanI BanII BbvCI Bme1580I BmrI BplI BpmI Bpu10I BsaAI BsaHI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BssHII BstEII BstXI BstYI BtgI DraIII DrdI EaeI EciI EcoICRI Eco57MI EcoO109I FalI HaeII MscI MslI MspA1I NcoI NspI PasI PfoI PmlI PspOMI PstI SacI SfcI SmaI StuI StyI TaqII XcmI XmaI ZraI Enzymes that do not cut: AarI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI BpuEI BsaI BsaBI BsaWI BsaXI BseRI BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI EagI EarI EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI Tth111I XbaI XhoI XmnI