(Circular) (Six-base) MAPSORT of: pFN21AE5546.seq Check: 7436 from: 1 to: 5872 pFN21AE5546 5872 bp With 182 enzymes: SgfI * December 8, 2014 13:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3682 278 Size: 53 83 186 936 2146 2468 Fragments arranged by size: 2468 2146 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3215 1937 Size: 1278 4594 Acc65I G'GTAC_C Cuts at: 3193 3193 Size: 5872 AclI AA'CG_TT Cuts at: 1563 3999 4372 1563 Size: 2436 373 3063 Fragments arranged by size: 3063 2436 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3935 5319 5587 1632 Size: 2303 1384 268 1917 Fragments arranged by size: 2303 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5675 AflIII A'CryG_T Cuts at: 1203 2844 4771 5748 1203 Size: 1641 1927 977 1327 Fragments arranged by size: 1927 1641 1327 977 AgeI A'CCGG_T Cuts at: 4703 4703 Size: 5872 AhdI GACnn_n'nnGTC Cuts at: 4601 4601 Size: 5872 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5872 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5872 AlwNI CAG_nnn'CTG Cuts at: 2584 3148 3615 5187 2584 Size: 564 467 1572 3269 Fragments arranged by size: 3269 1572 564 467 ApaI G_GGCC'C Cuts at: 2054 2208 2054 Size: 154 5718 ApaLI G'TGCA_C Cuts at: 2171 2711 3929 5085 2171 Size: 540 1218 1156 2958 Fragments arranged by size: 2958 1218 1156 540 ApoI r'AATT_y Cuts at: 1493 3181 3407 3559 5668 1493 Size: 1688 226 152 2109 1697 Fragments arranged by size: 2109 1697 1688 226 152 AscI GG'CGCG_CC Cuts at: 2065 2065 Size: 5872 AseI AT'TA_AT Cuts at: 160 1032 4426 160 Size: 872 3394 1606 Fragments arranged by size: 3394 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5872 AvaI C'yCGr_G Cuts at: 1870 1943 2053 3197 1870 Size: 73 110 1144 4545 Fragments arranged by size: 4545 1144 110 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2556 2589 2556 Size: 33 5839 BamHI G'GATC_C Cuts at: 3202 3569 3202 Size: 367 5505 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2272 2663 3193 4648 618 Size: 325 217 627 485 391 530 1455 1842 Fragments arranged by size: 1842 1455 627 530 485 391 325 217 BanII G_rGCy'C Cuts at: 1391 2054 2208 2631 2869 2892 2979 3191 1391 Size: 663 154 423 238 23 87 212 4072 Fragments arranged by size: 4072 663 423 238 212 154 87 23 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4901 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4063 4097 1903 Size: 34 2126 34 3678 Fragments arranged by size: 3678 2126 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2584 3764 4974 2584 Size: 1180 1210 3482 Fragments arranged by size: 3482 1210 1180 BclI T'GATC_A Cuts at: 1548 1548 Size: 5872 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2058 4483 136 Size: 107 122 71 631 605 386 2425 1525 Fragments arranged by size: 2425 1525 631 605 386 122 107 71 BglII A'GATC_T Cuts at: 5867 5867 Size: 5872 BlpI GC'TnA_GC Cuts at: 3289 3289 Size: 5872 Bme1580I G_kGCm'C Cuts at: 1790 2054 2175 2208 2546 2715 3933 5089 1790 Size: 264 121 33 338 169 1218 1156 2573 Fragments arranged by size: 2573 1218 1156 338 264 169 121 33 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5872 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2572 3603 4561 Size: 416 53 83 399 24 1155 1031 958 Cuts at: 4561 442 Size: 1753 Fragments arranged by size: 1753 1155 1031 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5872 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2521 2521 Size: 5872 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2723 3137 4532 1239 Size: 1484 414 1395 2579 Fragments arranged by size: 2579 1484 1395 414 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3997 4862 5160 3997 Size: 865 298 4709 Fragments arranged by size: 4709 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2297 4535 882 Size: 617 798 2238 2219 Fragments arranged by size: 2238 2219 798 617 BsaAI yAC'GTr Cuts at: 493 1204 2845 3663 493 Size: 711 1641 818 2702 Fragments arranged by size: 2702 1641 818 711 BsaBI GATnn'nnATC Cuts at: 5866 5866 Size: 5872 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3679 4061 4696 275 Size: 53 83 186 936 2146 382 635 1451 Fragments arranged by size: 2146 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2010 4305 4703 4977 5124 5535 2010 Size: 2295 398 274 147 411 2347 Fragments arranged by size: 2347 2295 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2102 2132 1178 Size: 30 165 30 699 30 4918 Fragments arranged by size: 4918 699 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2198 2240 1186 Size: 1012 42 4818 Fragments arranged by size: 4818 1012 42 BseYI C'CCAG_C Cuts at: 2399 2631 5075 5571 2399 Size: 232 2444 496 2700 Fragments arranged by size: 2700 2444 496 232 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2264 1163 Size: 573 528 4771 Fragments arranged by size: 4771 573 528 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3322 4083 5111 1540 Size: 275 186 1321 761 1028 2301 Fragments arranged by size: 2301 1321 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2175 2715 2892 3933 4018 5089 2175 Size: 540 177 1041 85 1071 2958 Fragments arranged by size: 2958 1071 1041 540 177 85 BsiWI C'GTAC_G Cuts at: 2871 2871 Size: 5872 BsmI GAATG_Cn' Cuts at: 2420 3383 3476 2420 Size: 963 93 4816 Fragments arranged by size: 4816 963 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2175 2208 2546 2631 2715 2869 Size: 399 264 121 33 338 85 84 154 Cuts at: 2869 2892 2979 3191 3933 4018 5089 1391 Size: 23 87 212 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 338 264 212 154 121 87 85 85 84 33 23 BspEI T'CCGG_A Cuts at: 2010 5535 2010 Size: 3525 2347 BspHI T'CATG_A Cuts at: 2799 3761 5491 2799 Size: 962 1730 3180 Fragments arranged by size: 3180 1730 962 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3227 844 Size: 2383 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3759 1394 Size: 429 1936 3507 Fragments arranged by size: 3507 1936 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2350 4367 4541 65 Size: 1183 1102 2017 174 1396 Fragments arranged by size: 2017 1396 1183 1102 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2838 4516 4703 1700 Size: 110 1028 1678 187 2869 Fragments arranged by size: 2869 1678 1028 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5872 BssHII G'CGCG_C Cuts at: 1925 2065 1925 Size: 140 5732 BssSI C'ACGA_G Cuts at: 2450 3932 4944 2450 Size: 1482 1012 3378 Fragments arranged by size: 3378 1482 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5872 BstBI TT'CG_AA Cuts at: 4676 4676 Size: 5872 BstEII G'GTnAC_C Cuts at: 1184 2470 1184 Size: 1286 4586 BstXI CCAn_nnnn'nTGG Cuts at: 3064 3106 3064 Size: 42 5830 BstYI r'GATC_y Cuts at: 1972 3202 3569 3954 3971 5423 5509 5521 5867 Size: 1230 367 385 17 1452 86 12 346 Cuts at: 5867 1972 Size: 1977 Fragments arranged by size: 1977 1452 1230 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2145 513 Size: 552 1080 4240 Fragments arranged by size: 4240 1080 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2100 2725 3174 487 Size: 683 56 874 625 449 3185 Fragments arranged by size: 3185 874 683 625 449 56 BtsI GCAGTG_nn' Cuts at: 3388 4182 4202 3388 Size: 794 20 5058 Fragments arranged by size: 5058 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5872 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5837 DraI TTT'AAA Cuts at: 3176 3176 Size: 5872 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2765 3038 4879 809 Size: 507 1449 273 1841 1802 Fragments arranged by size: 1841 1802 1449 507 273 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2113 2368 3319 Size: 54 1425 50 275 18 283 255 951 Cuts at: 3319 4208 5837 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 951 889 283 275 255 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3319 1537 Size: 275 1507 4090 Fragments arranged by size: 4090 1507 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3802 1353 Size: 2449 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2569 4459 4833 4979 387 Size: 1064 138 980 1890 374 146 1280 Fragments arranged by size: 1890 1280 1064 980 374 146 138 EcoICRI GAG'CTC Cuts at: 2890 2890 Size: 5872 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2723 3137 3935 4532 5319 5587 1239 Size: 393 1091 414 798 597 787 268 1524 Fragments arranged by size: 1524 1091 798 787 597 414 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2705 2705 Size: 5872 EcoO109I rG'GnC_Cy Cuts at: 2204 2205 2730 2204 Size: 1 525 5346 Fragments arranged by size: 5346 525 1 EcoRI G'AATT_C Cuts at: 3181 3559 3181 Size: 378 5494 FspI TGC'GCA Cuts at: 4378 4378 Size: 5872 HaeII r_GCGC'y Cuts at: 1678 5019 1678 Size: 3341 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4513 4545 4587 4619 1373 Size: 32 135 32 2941 32 42 32 2626 Fragments arranged by size: 2941 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3216 3462 669 Size: 988 281 1278 246 3079 Fragments arranged by size: 3079 1278 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5872 HpaI GTT'AAC Cuts at: 3462 3462 Size: 5872 KpnI G_GTAC'C Cuts at: 3197 3197 Size: 5872 MfeI C'AATT_G Cuts at: 3471 3471 Size: 5872 MluI A'CGCG_T Cuts at: 5748 5748 Size: 5872 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2145 2985 3045 4986 5170 1305 Size: 840 840 60 1941 184 2007 Fragments arranged by size: 2007 1941 840 840 184 60 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4050 Fragments arranged by size: 4050 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2178 2343 2654 3830 4189 4348 518 Size: 648 1012 165 311 1176 359 159 2042 Fragments arranged by size: 2042 1176 1012 648 359 311 165 159 MspA1I CmG'CkG Cuts at: 1606 2128 2279 3046 3288 3965 5113 5358 1606 Size: 522 151 767 242 677 1148 245 2120 Fragments arranged by size: 2120 1148 767 677 522 245 242 151 NcoI C'CATG_G Cuts at: 513 1065 2145 513 Size: 552 1080 4240 Fragments arranged by size: 4240 1080 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5872 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5872 NotI GC'GGCC_GC Cuts at: 3319 3319 Size: 5872 NspI r_CATG'y Cuts at: 1132 1229 2464 2693 3230 4775 1132 Size: 97 1235 229 537 1545 2229 Fragments arranged by size: 2229 1545 1235 537 229 97 PasI CC'CwG_GG Cuts at: 1348 3148 1348 Size: 1800 4072 PciI A'CATG_T Cuts at: 4771 4771 Size: 5872 PflMI CCAn_nnn'nTGG Cuts at: 1108 2256 1108 Size: 1148 4724 PfoI T'CCnGG_A Cuts at: 2700 3114 2700 Size: 414 5458 PmeI GTTT'AAAC Cuts at: 3176 3176 Size: 5872 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3941 3973 5454 5486 3941 Size: 32 1481 32 4327 Fragments arranged by size: 4327 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5872 PsiI TTA'TAA Cuts at: 3442 3442 Size: 5872 PspOMI G'GGCC_C Cuts at: 2050 2204 2050 Size: 154 5718 PstI C_TGCA'G Cuts at: 830 2032 2589 3224 830 Size: 1202 557 635 3478 Fragments arranged by size: 3478 1202 635 557 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5872 PvuII CAG'CTG Cuts at: 2128 2128 Size: 5872 SacI G_AGCT'C Cuts at: 2892 2892 Size: 5872 SalI G'TCGA_C Cuts at: 1936 3214 1936 Size: 1278 4594 SbfI CC_TGCA'GG Cuts at: 3224 3224 Size: 5872 ScaI AGT'ACT Cuts at: 4120 5832 4120 Size: 1712 4160 SexAI A'CCwGG_T Cuts at: 2913 2913 Size: 5872 SfcI C'TryA_G Cuts at: 826 1045 2028 2361 2585 3220 4355 5036 5227 Size: 219 983 333 224 635 1135 681 191 Cuts at: 5227 826 Size: 1471 Fragments arranged by size: 1471 1135 983 681 635 333 224 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5872 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5872 SmaI CCC'GGG Cuts at: 1872 2055 3199 1872 Size: 183 1144 4545 Fragments arranged by size: 4545 1144 183 SmlI C'TyrA_G Cuts at: 820 1017 1943 3976 4877 5139 820 Size: 197 926 2033 901 262 1553 Fragments arranged by size: 2033 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3663 493 Size: 3170 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5872 SphI G_CATG'C Cuts at: 1132 2464 3230 1132 Size: 1332 766 3774 Fragments arranged by size: 3774 1332 766 SspI AAT'ATT Cuts at: 5 52 3796 5 Size: 47 3744 2081 Fragments arranged by size: 3744 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5872 StyI C'CwwG_G Cuts at: 513 1065 2145 3099 3311 513 Size: 552 1080 954 212 3074 Fragments arranged by size: 3074 1080 954 552 212 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2138 2248 3899 3916 4069 4254 4699 Size: 1255 558 110 1651 17 153 185 445 Cuts at: 4699 325 Size: 1498 Fragments arranged by size: 1651 1498 1255 558 445 185 153 110 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4118 5830 96 Size: 274 80 33 51 1443 2141 1712 138 Fragments arranged by size: 2141 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2903 2935 5832 5864 2903 Size: 32 2897 32 2911 Fragments arranged by size: 2911 2897 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5872 XbaI T'CTAG_A Cuts at: 3208 3208 Size: 5872 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2257 2449 2615 2257 Size: 192 166 5514 Fragments arranged by size: 5514 192 166 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5872 XmaI C'CCGG_G Cuts at: 1870 2053 3197 1870 Size: 183 1144 4545 Fragments arranged by size: 4545 1144 183 XmnI GAAnn'nnTTC Cuts at: 4001 4001 Size: 5872 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3680 276 Size: 53 83 186 936 2146 2468 Fragments arranged by size: 2468 2146 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AvrII BbeI BbvCI BfrBI Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI