################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BbeI BbrPI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BseAI BseRI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AluI 210 AGCT AG^CT AlwNI 399 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 451 GTGCTC G[AT]GC[AT]^C AvaII 269 GGTCC G^G[AT]CC BamHI 238 GGATCC G^GATCC BbsI 261 GTCTTC GAAGAC(2/6) BclI 297 TGATCA T^GATCA BcnI 419 CCCGG CC^[GC]GG BpmI 575 CTGGAG CTGGAG(16/14) BpuAI 261 GTCTTC GAAGAC(2/6) BsaI 304 GAGACC GGTCTC(1/5) BsaWI 235 ACCGGA [AT]^CCGG[AT] BsiHKAI 451 GTGCTC G[AT]GC[AT]^C BsmBI 329 CGTCTC CGTCTC(1/5) BsmFI 270 GTCCC GGGAC(10/14) BspMI 502 GCAGGT ACCTGC(4/8) BsrDI 473 CATTGC GCAATG(2/0) BstAPI 156 GCAGCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 238 GGATCC [GA]^GATC[CT] DdeI 319 CTCAG C^T[ACGT]AG DsaI 117 CCATGG C^C[GA][CT]GG EarI 256 GAAGAG CTCTTC(1/4) EcoT22I 470 ATGCAT ATGCA^T FbaI 297 TGATCA T^GATCA HinfI 259 GAGTC G^A[ACGT]TC HphI 6 GGTGA GGTGA(8/7) Ksp632I 256 GAAGAG CTCTTC(1/4) MaeII 328 ACGT A^CGT MaeIII 356 GTGAC ^GT[ACGT]AC MflI 238 GGATCC [GA]^GATC[CT] MslI 349 CATTCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 434 CCGCTG C[AC]G^C[GT]G NciI 419 CCCGG CC^[GC]GG NcoI 117 CCATGG C^CATGG NsiI 470 ATGCAT ATGCA^T NspI 468 ACATGC [GA]CATG^[CT] PflMI 217 CCAACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 259 GAGTC GAGTC(4/5) PpuMI 268 AGGTCCC [GA]G^G[AT]CC[CT] SmlI 482 CTCAAG C^T[CT][GA]AG Tsp45I 356 GTGAC ^GT[GC]AC Van91I 217 CCAACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 238 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AlwI 239 GATCC GGATC(4/5) 238 GGATC ApaI 182 GGGCCC GGGCC^C 394 GGGCCC BalI 32 TGGCCA TGG^CCA 50 TGGCCA BanII 182 GGGCCC G[GA]GC[CT]^C 394 GGGCCC BsgI 491 CTGCAC GTGCAG(16/14) 506 GTGCAG BsmAI 304 GAGAC GTCTC(1/5) 330 GTCTC BsrI 308 CCAGT ACTGG(1/-1) 448 CCAGT EaeI 32 TGGCCA [CT]^GGCC[GA] 50 TGGCCA EcoT14I 117 CCATGG C^C[AT][AT]GG 334 CCTTGG FauI 153 CCCGC CCCGC(4/6) 433 CCCGC HapII 236 CCGG C^CGG 420 CCGG HpaII 236 CCGG C^CGG 420 CCGG MluNI 32 TGGCCA TGG^CCA 50 TGGCCA MscI 32 TGGCCA TGG^CCA 50 TGGCCA MseI 14 TTAA T^TAA 89 TTAA MspI 236 CCGG C^CGG 420 CCGG PstI 75 CTGCAG CTGCA^G 386 CTGCAG StyI 117 CCATGG C^C[AT][AT]GG 334 CCTTGG Tru9I 14 TTAA T^TAA 89 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AciI BanI BbvI BfaI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII ItaI MaeI MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI SfcI TseI TspRI