(Circular) (Six-base) MAPSORT of: pFN21AB6868.seq Check: 9749 from: 1 to: 5293 pFN21AB6868 5293 bp With 182 enzymes: SgfI * March 28, 2012 14:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2498 2498 Size: 5293 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3103 278 Size: 53 83 186 936 1567 2468 Fragments arranged by size: 2468 1567 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2636 1937 Size: 699 4594 Acc65I G'GTAC_C Cuts at: 2614 2614 Size: 5293 AclI AA'CG_TT Cuts at: 1563 3420 3793 1563 Size: 1857 373 3063 Fragments arranged by size: 3063 1857 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3356 4740 5008 1632 Size: 1724 1384 268 1917 Fragments arranged by size: 1917 1724 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5096 AflIII A'CryG_T Cuts at: 1203 4192 5169 1203 Size: 2989 977 1327 Fragments arranged by size: 2989 1327 977 AgeI A'CCGG_T Cuts at: 4124 4124 Size: 5293 AhdI GACnn_n'nnGTC Cuts at: 4022 4022 Size: 5293 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5293 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5293 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2532 2564 2532 Size: 32 5261 AlwNI CAG_nnn'CTG Cuts at: 2409 3036 4608 2409 Size: 627 1572 3094 Fragments arranged by size: 3094 1572 627 ApaI G_GGCC'C Cuts at: 2191 2403 2191 Size: 212 5081 ApaLI G'TGCA_C Cuts at: 3350 4506 3350 Size: 1156 4137 ApoI r'AATT_y Cuts at: 1493 2602 2828 2980 5089 1493 Size: 1109 226 152 2109 1697 Fragments arranged by size: 2109 1697 1109 226 152 AseI AT'TA_AT Cuts at: 160 1032 3847 160 Size: 872 2815 1606 Fragments arranged by size: 2815 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5293 AvaI C'yCGr_G Cuts at: 1870 1943 2618 1870 Size: 73 675 4545 Fragments arranged by size: 4545 675 73 BamHI G'GATC_C Cuts at: 2243 2623 2990 2243 Size: 380 367 4546 Fragments arranged by size: 4546 380 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2154 2200 2343 2420 2614 Size: 325 217 627 367 46 143 77 194 Cuts at: 2614 4069 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 367 325 217 194 143 77 46 BanII G_rGCy'C Cuts at: 1391 2191 2403 2612 1391 Size: 800 212 209 4072 Fragments arranged by size: 4072 800 212 209 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2259 928 Size: 971 360 3962 Fragments arranged by size: 3962 971 360 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3484 3518 1903 Size: 34 1547 34 3678 Fragments arranged by size: 3678 1547 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3185 4395 3185 Size: 1210 4083 BclI T'GATC_A Cuts at: 1548 2302 1548 Size: 754 4539 BfrBI ATG'CAT Cuts at: 2477 2477 Size: 5293 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3904 136 Size: 107 122 71 631 605 2232 1525 Fragments arranged by size: 2232 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5288 5288 Size: 5293 BlpI GC'TnA_GC Cuts at: 2710 2710 Size: 5293 Bme1580I G_kGCm'C Cuts at: 1790 2159 2191 2205 2403 2425 3354 4510 1790 Size: 369 32 14 198 22 929 1156 2573 Fragments arranged by size: 2573 1156 929 369 198 32 22 14 BmgBI CAC'GTC Cuts at: 1318 2334 1318 Size: 1016 4277 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3024 3982 442 Size: 416 53 83 399 24 1607 958 1753 Fragments arranged by size: 1753 1607 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5293 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2601 3953 1239 Size: 1362 1352 2579 Fragments arranged by size: 2579 1362 1352 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2472 3418 4283 4581 2472 Size: 946 865 298 3184 Fragments arranged by size: 3184 946 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2303 3956 882 Size: 617 804 1653 2219 Fragments arranged by size: 2219 1653 804 617 BsaAI yAC'GTr Cuts at: 493 1204 3084 493 Size: 711 1880 2702 Fragments arranged by size: 2702 1880 711 BsaBI GATnn'nnATC Cuts at: 5287 5287 Size: 5293 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3100 3482 4117 275 Size: 53 83 186 936 1567 382 635 1451 Fragments arranged by size: 1567 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2240 3726 4124 4398 4545 4956 2240 Size: 1486 398 274 147 411 2577 Fragments arranged by size: 2577 1486 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5068 Fragments arranged by size: 5068 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5293 BseYI C'CCAG_C Cuts at: 4496 4992 4496 Size: 496 4797 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2481 2532 1163 Size: 573 745 51 3924 Fragments arranged by size: 3924 745 573 51 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2743 3504 4532 1540 Size: 275 186 742 761 1028 2301 Fragments arranged by size: 2301 1028 761 742 275 186 BsiHKAI G_wGCw'C Cuts at: 2460 3354 3439 4510 2460 Size: 894 85 1071 3243 Fragments arranged by size: 3243 1071 894 85 BsmI GAATG_Cn' Cuts at: 2804 2897 2804 Size: 93 5200 BsmBI CGTCTCn'nnnn_ Cuts at: 2340 2340 Size: 5293 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2159 2191 2205 2403 2425 2460 2612 Size: 399 369 32 14 198 22 35 152 Cuts at: 2612 3354 3439 4510 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 369 198 152 85 35 32 22 14 BspEI T'CCGG_A Cuts at: 4956 4956 Size: 5293 BspHI T'CATG_A Cuts at: 3182 4912 3182 Size: 1730 3563 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2498 2648 844 Size: 1654 150 3489 Fragments arranged by size: 3489 1654 150 BsrBI CCG'CTC Cuts at: 1394 1823 3180 1394 Size: 429 1357 3507 Fragments arranged by size: 3507 1357 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2477 3788 3962 65 Size: 1183 1229 1311 174 1396 Fragments arranged by size: 1396 1311 1229 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3937 4124 1700 Size: 110 2127 187 2869 Fragments arranged by size: 2869 2127 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5293 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5293 BssSI C'ACGA_G Cuts at: 3353 4365 3353 Size: 1012 4281 BstAPI GCAn_nnn'nTGC Cuts at: 794 2167 794 Size: 1373 3920 BstBI TT'CG_AA Cuts at: 4097 4097 Size: 5293 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5293 BstYI r'GATC_y Cuts at: 1972 2243 2623 2990 3375 3392 4844 4930 4942 Size: 271 380 367 385 17 1452 86 12 Cuts at: 4942 5288 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 380 367 346 271 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2122 513 Size: 552 1057 3684 Fragments arranged by size: 3684 1057 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4554 Fragments arranged by size: 4554 683 56 BtsI GCAGTG_nn' Cuts at: 2462 2519 2809 3603 3623 2462 Size: 57 290 794 20 4132 Fragments arranged by size: 4132 794 290 57 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5293 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2460 2495 529 Size: 35 1896 35 3327 Fragments arranged by size: 3327 1896 35 35 DraI TTT'AAA Cuts at: 2597 2597 Size: 5293 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4300 809 Size: 507 2984 1802 Fragments arranged by size: 2984 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 2055 2740 Size: 54 1425 50 275 18 207 18 685 Cuts at: 2740 3629 5258 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 685 275 207 54 50 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 2740 1537 Size: 275 928 4090 Fragments arranged by size: 4090 928 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2256 3223 1353 Size: 903 967 3423 Fragments arranged by size: 3423 967 903 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3880 4254 4400 387 Size: 1064 138 2291 374 146 1280 Fragments arranged by size: 2291 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2601 3356 3953 4740 5008 1239 Size: 393 969 755 597 787 268 1524 Fragments arranged by size: 1524 969 787 755 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2187 2274 2406 2187 Size: 87 132 5074 Fragments arranged by size: 5074 132 87 EcoRI G'AATT_C Cuts at: 2602 2980 2602 Size: 378 4915 FspI TGC'GCA Cuts at: 3799 3799 Size: 5293 HaeII r_GCGC'y Cuts at: 1678 4440 1678 Size: 2762 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2372 2404 3934 3966 4008 Size: 32 135 32 800 32 1530 32 42 Cuts at: 4008 4040 1373 Size: 32 2626 Fragments arranged by size: 2626 1530 800 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2637 2883 669 Size: 988 281 699 246 3079 Fragments arranged by size: 3079 988 699 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5293 HpaI GTT'AAC Cuts at: 2883 2883 Size: 5293 KpnI G_GTAC'C Cuts at: 2618 2618 Size: 5293 MfeI C'AATT_G Cuts at: 2892 2892 Size: 5293 MluI A'CGCG_T Cuts at: 5169 5169 Size: 5293 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4407 4591 1305 Size: 3102 184 2007 Fragments arranged by size: 3102 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2039 2057 10 Size: 54 1425 343 207 18 3246 Fragments arranged by size: 3246 1425 343 207 54 18 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2358 3251 3610 3769 518 Size: 648 843 349 893 359 159 2042 Fragments arranged by size: 2042 893 843 648 359 349 159 MspA1I CmG'CkG Cuts at: 1606 2441 2709 3386 4534 4779 1606 Size: 835 268 677 1148 245 2120 Fragments arranged by size: 2120 1148 835 677 268 245 NcoI C'CATG_G Cuts at: 513 1065 2122 513 Size: 552 1057 3684 Fragments arranged by size: 3684 1057 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5293 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5293 NotI GC'GGCC_GC Cuts at: 2740 2740 Size: 5293 NsiI A_TGCA'T Cuts at: 2479 2479 Size: 5293 NspI r_CATG'y Cuts at: 1132 1229 2477 2651 4196 1132 Size: 97 1248 174 1545 2229 Fragments arranged by size: 2229 1545 1248 174 97 PasI CC'CwG_GG Cuts at: 1348 2403 1348 Size: 1055 4238 PciI A'CATG_T Cuts at: 4192 4192 Size: 5293 PflMI CCAn_nnn'nTGG Cuts at: 1108 2228 1108 Size: 1120 4173 PmeI GTTT'AAAC Cuts at: 2597 2597 Size: 5293 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3362 3394 4875 4907 3362 Size: 32 1481 32 3748 Fragments arranged by size: 3748 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2274 2274 Size: 5293 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5293 PsiI TTA'TAA Cuts at: 2863 2863 Size: 5293 PspOMI G'GGCC_C Cuts at: 2187 2399 2187 Size: 212 5081 PstI C_TGCA'G Cuts at: 830 2084 2395 2645 830 Size: 1254 311 250 3478 Fragments arranged by size: 3478 1254 311 250 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5293 SalI G'TCGA_C Cuts at: 1936 2635 1936 Size: 699 4594 SbfI CC_TGCA'GG Cuts at: 2645 2645 Size: 5293 ScaI AGT'ACT Cuts at: 3541 5253 3541 Size: 1712 3581 SfcI C'TryA_G Cuts at: 826 1045 2080 2391 2410 2641 3776 4457 4648 Size: 219 1035 311 19 231 1135 681 191 Cuts at: 4648 826 Size: 1471 Fragments arranged by size: 1471 1135 1035 681 311 231 219 191 19 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5293 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5293 SmaI CCC'GGG Cuts at: 1872 2620 1872 Size: 748 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2487 3397 4298 4560 820 Size: 197 926 544 910 901 262 1553 Fragments arranged by size: 1553 926 910 901 544 262 197 SnaBI TAC'GTA Cuts at: 493 3084 493 Size: 2591 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5293 SphI G_CATG'C Cuts at: 1132 2651 1132 Size: 1519 3774 SspI AAT'ATT Cuts at: 5 52 3217 5 Size: 47 3165 2081 Fragments arranged by size: 3165 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5293 StyI C'CwwG_G Cuts at: 513 1065 2122 2339 2732 513 Size: 552 1057 217 393 3074 Fragments arranged by size: 3074 1057 552 393 217 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2041 3320 3337 3490 3675 4120 325 Size: 1255 461 1279 17 153 185 445 1498 Fragments arranged by size: 1498 1279 1255 461 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3539 5251 96 Size: 274 80 33 51 1443 1562 1712 138 Fragments arranged by size: 1712 1562 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2228 2260 5253 5285 2228 Size: 32 2993 32 2236 Fragments arranged by size: 2993 2236 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5293 XbaI T'CTAG_A Cuts at: 2629 2629 Size: 5293 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5293 XmaI C'CCGG_G Cuts at: 1870 2618 1870 Size: 748 4545 XmnI GAAnn'nnTTC Cuts at: 3422 3422 Size: 5293 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3101 276 Size: 53 83 186 936 1567 2468 Fragments arranged by size: 2468 1567 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI