(Circular) (Six-base) MAPSORT of: pFN21AE5269.seq Check: 3937 from: 1 to: 5299 pFN21AE5269 5299 bp With 182 enzymes: SgfI * January 30, 2014 17:16 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3109 278 Size: 53 83 186 936 1573 2468 Fragments arranged by size: 2468 1573 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2642 1937 Size: 705 4594 Acc65I G'GTAC_C Cuts at: 2620 2620 Size: 5299 AclI AA'CG_TT Cuts at: 1563 2270 3426 3799 1563 Size: 707 1156 373 3063 Fragments arranged by size: 3063 1156 707 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2282 3362 4746 5014 1632 Size: 650 1080 1384 268 1917 Fragments arranged by size: 1917 1384 1080 650 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5102 AflIII A'CryG_T Cuts at: 1203 4198 5175 1203 Size: 2995 977 1327 Fragments arranged by size: 2995 1327 977 AgeI A'CCGG_T Cuts at: 4130 4130 Size: 5299 AhdI GACnn_n'nnGTC Cuts at: 4028 4028 Size: 5299 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5299 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5299 AlwNI CAG_nnn'CTG Cuts at: 2203 3042 4614 2203 Size: 839 1572 2888 Fragments arranged by size: 2888 1572 839 ApaLI G'TGCA_C Cuts at: 2154 3356 4512 2154 Size: 1202 1156 2941 Fragments arranged by size: 2941 1202 1156 ApoI r'AATT_y Cuts at: 1493 2608 2834 2986 5095 1493 Size: 1115 226 152 2109 1697 Fragments arranged by size: 2109 1697 1115 226 152 AseI AT'TA_AT Cuts at: 160 1032 3853 160 Size: 872 2821 1606 Fragments arranged by size: 2821 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5299 AvaI C'yCGr_G Cuts at: 1870 1943 2624 1870 Size: 73 681 4545 Fragments arranged by size: 4545 681 73 BamHI G'GATC_C Cuts at: 2629 2996 2629 Size: 367 4932 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2086 2582 2620 4075 618 Size: 325 217 627 299 496 38 1455 1842 Fragments arranged by size: 1842 1455 627 496 325 299 217 38 BanII G_rGCy'C Cuts at: 1391 2259 2618 1391 Size: 868 359 4072 Fragments arranged by size: 4072 868 359 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4328 BbvCI CC'TCA_GC Cuts at: 2541 2541 Size: 5299 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2458 2492 3490 3524 1903 Size: 34 50 34 437 34 998 34 3678 Fragments arranged by size: 3678 998 437 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3191 4401 3191 Size: 1210 4089 BclI T'GATC_A Cuts at: 1548 2439 1548 Size: 891 4408 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2348 2570 3910 Size: 107 122 71 631 605 676 222 1340 Cuts at: 3910 136 Size: 1525 Fragments arranged by size: 1525 1340 676 631 605 222 122 107 71 BglII A'GATC_T Cuts at: 5294 5294 Size: 5299 BlpI GC'TnA_GC Cuts at: 2716 2716 Size: 5299 Bme1580I G_kGCm'C Cuts at: 1790 2158 3360 4516 1790 Size: 368 1202 1156 2573 Fragments arranged by size: 2573 1202 1156 368 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5299 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3030 3988 442 Size: 416 53 83 399 24 1613 958 1753 Fragments arranged by size: 1753 1613 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5299 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2588 2615 3959 1239 Size: 1349 27 1344 2579 Fragments arranged by size: 2579 1349 1344 27 Bpu10I CC'TnA_GC Cuts at: 2541 2541 Size: 5299 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3424 4289 4587 3424 Size: 865 298 4136 Fragments arranged by size: 4136 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3962 882 Size: 617 2463 2219 Fragments arranged by size: 2463 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3090 493 Size: 711 1886 2702 Fragments arranged by size: 2702 1886 711 BsaBI GATnn'nnATC Cuts at: 5293 5293 Size: 5299 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3106 3488 4123 275 Size: 53 83 186 936 1573 382 635 1451 Fragments arranged by size: 1573 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3732 4130 4404 4551 4962 3732 Size: 398 274 147 411 4069 Fragments arranged by size: 4069 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5074 Fragments arranged by size: 5074 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2131 1186 Size: 945 4354 BseYI C'CCAG_C Cuts at: 4502 4998 4502 Size: 496 4803 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4726 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2019 2749 3510 4538 1540 Size: 275 186 18 730 761 1028 2301 Fragments arranged by size: 2301 1028 761 730 275 186 18 BsiHKAI G_wGCw'C Cuts at: 2158 2259 3360 3445 4516 2158 Size: 101 1101 85 1071 2941 Fragments arranged by size: 2941 1101 1071 101 85 BsmI GAATG_Cn' Cuts at: 2810 2903 2810 Size: 93 5206 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2158 2259 2618 3360 3445 4516 1391 Size: 399 368 101 359 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 368 359 101 85 BspEI T'CCGG_A Cuts at: 4962 4962 Size: 5299 BspHI T'CATG_A Cuts at: 3188 4918 3188 Size: 1730 3569 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2654 844 Size: 1810 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2021 3186 1394 Size: 429 198 1165 3507 Fragments arranged by size: 3507 1165 429 198 BsrDI GCAATG_nn' Cuts at: 65 1248 3794 3968 65 Size: 1183 2546 174 1396 Fragments arranged by size: 2546 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2011 3943 4130 1700 Size: 110 201 1932 187 2869 Fragments arranged by size: 2869 1932 201 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5299 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5299 BssSI C'ACGA_G Cuts at: 3359 4371 3359 Size: 1012 4287 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5299 BstBI TT'CG_AA Cuts at: 4103 4103 Size: 5299 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5299 BstYI r'GATC_y Cuts at: 1972 2277 2629 2996 3381 3398 4850 4936 4948 Size: 305 352 367 385 17 1452 86 12 Cuts at: 4948 5294 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 352 346 305 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2421 2586 513 Size: 552 1356 165 3226 Fragments arranged by size: 3226 1356 552 165 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2200 487 Size: 683 56 974 3586 Fragments arranged by size: 3586 974 683 56 BtsI GCAGTG_nn' Cuts at: 2815 3609 3629 2815 Size: 794 20 4485 Fragments arranged by size: 4485 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5299 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5264 DraI TTT'AAA Cuts at: 2603 2603 Size: 5299 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4306 809 Size: 507 2990 1802 Fragments arranged by size: 2990 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2016 2125 2746 Size: 54 1425 50 275 18 186 109 621 Cuts at: 2746 3635 5264 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 621 275 186 109 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2016 2746 1537 Size: 275 204 730 4090 Fragments arranged by size: 4090 730 275 204 EarI CTCTTCn'nnn_ Cuts at: 1353 3229 1353 Size: 1876 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2057 2390 2561 3886 4260 4406 Size: 1064 138 468 333 171 1325 374 146 Cuts at: 4406 387 Size: 1280 Fragments arranged by size: 1325 1280 1064 468 374 333 171 146 138 EcoICRI GAG'CTC Cuts at: 2257 2257 Size: 5299 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2282 2588 2615 3362 3959 4746 5014 Size: 393 650 306 27 747 597 787 268 Cuts at: 5014 1239 Size: 1524 Fragments arranged by size: 1524 787 747 650 597 393 306 268 27 EcoNI CCTnn'n_nnAGG Cuts at: 2408 2480 2510 2408 Size: 72 30 5197 Fragments arranged by size: 5197 72 30 EcoO109I rG'GnC_Cy Cuts at: 2036 2143 2036 Size: 107 5192 EcoRI G'AATT_C Cuts at: 2608 2986 2608 Size: 378 4921 EcoRV GAT'ATC Cuts at: 2365 2365 Size: 5299 FspI TGC'GCA Cuts at: 3805 3805 Size: 5299 HaeII r_GCGC'y Cuts at: 1678 4446 1678 Size: 2768 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3940 3972 4014 4046 1373 Size: 32 135 32 2368 32 42 32 2626 Fragments arranged by size: 2626 2368 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2643 2889 669 Size: 988 281 705 246 3079 Fragments arranged by size: 3079 988 705 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5299 HpaI GTT'AAC Cuts at: 2889 2889 Size: 5299 KpnI G_GTAC'C Cuts at: 2624 2624 Size: 5299 MfeI C'AATT_G Cuts at: 2898 2898 Size: 5299 MluI A'CGCG_T Cuts at: 5175 5175 Size: 5299 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4413 4597 1305 Size: 3108 184 2007 Fragments arranged by size: 3108 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3477 Fragments arranged by size: 3477 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3257 3616 3775 518 Size: 648 2091 359 159 2042 Fragments arranged by size: 2091 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2060 2124 2203 2715 3392 4540 4785 1606 Size: 454 64 79 512 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 512 454 245 79 64 NaeI GCC'GGC Cuts at: 2013 2013 Size: 5299 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4747 NdeI CA'TA_TG Cuts at: 387 2112 387 Size: 1725 3574 NgoMIV G'CCGG_C Cuts at: 2011 2011 Size: 5299 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5299 NotI GC'GGCC_GC Cuts at: 2016 2746 2016 Size: 730 4569 NspI r_CATG'y Cuts at: 1132 1229 2657 4202 1132 Size: 97 1428 1545 2229 Fragments arranged by size: 2229 1545 1428 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5299 PciI A'CATG_T Cuts at: 4198 4198 Size: 5299 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5299 PfoI T'CCnGG_A Cuts at: 2208 2280 2208 Size: 72 5227 PmeI GTTT'AAAC Cuts at: 2603 2603 Size: 5299 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3368 3400 4881 4913 3368 Size: 32 1481 32 3754 Fragments arranged by size: 3754 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5299 PsiI TTA'TAA Cuts at: 2869 2869 Size: 5299 PstI C_TGCA'G Cuts at: 830 2338 2651 830 Size: 1508 313 3478 Fragments arranged by size: 3478 1508 313 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5299 PvuII CAG'CTG Cuts at: 2203 2203 Size: 5299 SacI G_AGCT'C Cuts at: 2259 2259 Size: 5299 SalI G'TCGA_C Cuts at: 1936 2641 1936 Size: 705 4594 SbfI CC_TGCA'GG Cuts at: 2651 2651 Size: 5299 ScaI AGT'ACT Cuts at: 3547 5259 3547 Size: 1712 3587 SfcI C'TryA_G Cuts at: 826 1045 2334 2647 3782 4463 4654 826 Size: 219 1289 313 1135 681 191 1471 Fragments arranged by size: 1471 1289 1135 681 313 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5299 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5299 SmaI CCC'GGG Cuts at: 1872 2626 1872 Size: 754 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3403 4304 4566 820 Size: 197 926 1460 901 262 1553 Fragments arranged by size: 1553 1460 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3090 493 Size: 2597 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5299 SphI G_CATG'C Cuts at: 1132 2657 1132 Size: 1525 3774 SspI AAT'ATT Cuts at: 5 52 3223 5 Size: 47 3171 2081 Fragments arranged by size: 3171 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5299 StyI C'CwwG_G Cuts at: 513 1065 2044 2175 2464 2738 513 Size: 552 979 131 289 274 3074 Fragments arranged by size: 3074 979 552 289 274 131 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2294 2423 3326 3343 3496 3681 4126 Size: 1255 714 129 903 17 153 185 445 Cuts at: 4126 325 Size: 1498 Fragments arranged by size: 1498 1255 903 714 445 185 153 129 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3545 5257 96 Size: 274 80 33 51 1443 1568 1712 138 Fragments arranged by size: 1712 1568 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5259 5291 5259 Size: 32 5267 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5299 XbaI T'CTAG_A Cuts at: 2635 2635 Size: 5299 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5299 XmaI C'CCGG_G Cuts at: 1870 2624 1870 Size: 754 4545 XmnI GAAnn'nnTTC Cuts at: 3428 3428 Size: 5299 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3107 276 Size: 53 83 186 936 1573 2468 Fragments arranged by size: 2468 1573 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI