Restriction map for
pFN21AE3344
(Circular) (Six-base) MAPSORT of: pFN21AE3344.seq Check: 1648 from: 1 to: 7717
pFN21AE3344 7717 bp
With 182 enzymes: SgfI *
August 14, 2013 14:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3157 4081 3157
Size: 924 6793
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2301 4719 5527 278
Size: 53 83 186 936 765 2418 808 2468
Fragments arranged by size:
2468 2418 936 808 765 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2301 5060 1937
Size: 364 2759 4594
Fragments arranged by size:
4594 2759 364
Acc65I G'GTAC_C
Cuts at: 5038 5038
Size: 7717
AclI AA'CG_TT
Cuts at: 1563 5844 6217 1563
Size: 4281 373 3063
Fragments arranged by size:
4281 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2255 2300 2323 2606 2711 2825 5780 7164
Size: 623 45 23 283 105 114 2955 1384
Cuts at: 7164 7432 1632
Size: 268 1917
Fragments arranged by size:
2955 1917 1384 623 283 268 114 105
45 23
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7520
AflIII A'CryG_T
Cuts at: 1203 6616 7593 1203
Size: 5413 977 1327
Fragments arranged by size:
5413 1327 977
AgeI A'CCGG_T
Cuts at: 6548 6548
Size: 7717
AhdI GACnn_n'nnGTC
Cuts at: 2776 6446 2776
Size: 3670 4047
AleI CACnn'nnGTG
Cuts at: 1166 4442 1166
Size: 3276 4441
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2645 1184
Size: 1461 6256
AlwNI CAG_nnn'CTG
Cuts at: 2470 2701 2755 2875 2905 2992 3139 3295 3336
Size: 231 54 120 30 87 147 156 41
Cuts at: 3336 3454 3507 4006 4312 4734 4852 5460 7032
Size: 118 53 499 306 422 118 608 1572
Cuts at: 7032 2470
Size: 3155
Fragments arranged by size:
3155 1572 608 499 422 306 231 156
147 120 118 118 87 54 53 41
30
ApaLI G'TGCA_C
Cuts at: 4270 5774 6930 4270
Size: 1504 1156 5057
Fragments arranged by size:
5057 1504 1156
ApoI r'AATT_y
Cuts at: 1493 5026 5252 5404 7513 1493
Size: 3533 226 152 2109 1697
Fragments arranged by size:
3533 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6271 160
Size: 872 5239 1606
Fragments arranged by size:
5239 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7717
AvaI C'yCGr_G
Cuts at: 1870 1943 2241 3532 3802 3835 4027 5013 5042
Size: 73 298 1291 270 33 192 986 29
Cuts at: 5042 1870
Size: 4545
Fragments arranged by size:
4545 1291 986 298 270 192 73 33
29
BamHI G'GATC_C
Cuts at: 3696 5047 5414 3696
Size: 1351 367 5999
Fragments arranged by size:
5999 1351 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2460 3839 4202 4444 4832
Size: 325 217 627 673 1379 363 242 388
Cuts at: 4832 4891 5038 6493 618
Size: 59 147 1455 1842
Fragments arranged by size:
1842 1455 1379 673 627 388 363 325
242 217 147 59
BanII G_rGCy'C
Cuts at: 1391 3273 3541 3585 3809 3879 4228 4406 5012
Size: 1882 268 44 224 70 349 178 606
Cuts at: 5012 5036 1391
Size: 24 4072
Fragments arranged by size:
4072 1882 606 349 268 224 178 70
44 24
BbeI G_GCGC'C
Cuts at: 4895 4895
Size: 7717
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2243 928
Size: 971 344 6402
Fragments arranged by size:
6402 971 344
BbvCI CC'TCA_GC
Cuts at: 2579 2579
Size: 7717
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2483 2517 5908 5942 1903
Size: 34 546 34 3391 34 3678
Fragments arranged by size:
3678 3391 546 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4180 5609 6819 4180
Size: 1429 1210 5078
Fragments arranged by size:
5078 1429 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7717
BfrBI ATG'CAT
Cuts at: 3307 3737 3307
Size: 430 7287
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2210 4019 6328
Size: 107 122 71 631 605 538 1809 2309
Cuts at: 6328 136
Size: 1525
Fragments arranged by size:
2309 1809 1525 631 605 538 122 107
71
BglII A'GATC_T
Cuts at: 7712 7712
Size: 7717
BlpI GC'TnA_GC
Cuts at: 2600 4227 4949 5134 2600
Size: 1627 722 185 5183
Fragments arranged by size:
5183 1627 722 185
Bme1580I G_kGCm'C
Cuts at: 1790 2220 3842 4274 4449 4506 4835 5778 6934
Size: 430 1622 432 175 57 329 943 1156
Cuts at: 6934 1790
Size: 2573
Fragments arranged by size:
2573 1622 1156 943 432 430 329 175
57
BmgBI CAC'GTC
Cuts at: 1318 3390 4117 4777 1318
Size: 2072 727 660 4258
Fragments arranged by size:
4258 2072 727 660
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2980 5448 6406
Size: 416 53 83 399 24 1563 2468 958
Cuts at: 6406 442
Size: 1753
Fragments arranged by size:
2468 1753 1563 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7717
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3169 3169
Size: 7717
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2291 2543 2885 3406 3533 3764 3917 3932
Size: 1052 252 342 521 127 231 153 15
Cuts at: 3932 3983 4028 4204 4390 4624 4874 4948 6377
Size: 51 45 176 186 234 250 74 1429
Cuts at: 6377 1239
Size: 2579
Fragments arranged by size:
2579 1429 1052 521 342 252 250 234
231 186 176 153 127 74 51 45
15
Bpu10I CC'TnA_GC
Cuts at: 2579 3552 3701 4526 2579
Size: 973 149 825 5770
Fragments arranged by size:
5770 973 825 149
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3230 4600 4793 5842 6707 7005 3230
Size: 1370 193 1049 865 298 3942
Fragments arranged by size:
3942 1370 1049 865 298 193
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2360 2489 3081 3522 4872 6380 882
Size: 617 861 129 592 441 1350 1508 2219
Fragments arranged by size:
2219 1508 1350 861 617 592 441 129
BsaAI yAC'GTr
Cuts at: 493 1204 4413 5508 493
Size: 711 3209 1095 2702
Fragments arranged by size:
3209 2702 1095 711
BsaBI GATnn'nnATC
Cuts at: 4754 7711 4754
Size: 2957 4760
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2298 3666 4716 4892
Size: 53 83 186 936 765 1368 1050 176
Cuts at: 4892 5524 5906 6541 275
Size: 632 382 635 1451
Fragments arranged by size:
1451 1368 1050 936 765 635 632 382
186 176 83 53
BsaWI w'CCGG_w
Cuts at: 6150 6548 6822 6969 7380 6150
Size: 398 274 147 411 6487
Fragments arranged by size:
6487 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2045 2075 2666 2696 4527
Size: 30 165 30 642 30 591 30 1831
Cuts at: 4527 4557 1178
Size: 30 4338
Fragments arranged by size:
4338 1831 642 591 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 2180 2183 2429 2690 2804 2825 3929
Size: 880 114 3 246 261 114 21 1104
Cuts at: 3929 4487 4535 4604 4856 4886 1186
Size: 558 48 69 252 30 4017
Fragments arranged by size:
4017 1104 880 558 261 252 246 114
114 69 48 30 21 3
BseYI C'CCAG_C
Cuts at: 2111 2390 3440 4388 4656 6920 7416 2111
Size: 279 1050 948 268 2264 496 2412
Fragments arranged by size:
2412 2264 1050 948 496 279 268
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2162 2498 2597 4097 4720 4727 1163
Size: 573 426 336 99 1500 623 7 4153
Fragments arranged by size:
4153 1500 623 573 426 336 99 7
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5167 5928 6956 1540
Size: 275 186 3166 761 1028 2301
Fragments arranged by size:
3166 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2481 3118 3246 3585 4274 4497 5778 5863 6934
Size: 637 128 339 689 223 1281 85 1071
Cuts at: 6934 2481
Size: 3264
Fragments arranged by size:
3264 1281 1071 689 637 339 223 128
85
BsiWI C'GTAC_G
Cuts at: 2095 2095
Size: 7717
BsmI GAATG_Cn'
Cuts at: 3052 5228 5321 3052
Size: 2176 93 5448
Fragments arranged by size:
5448 2176 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3318 4783 3318
Size: 1465 6252
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2220 2481 3118 3246 3273 3541 3585
Size: 399 430 261 637 128 27 268 44
Cuts at: 3585 3809 3842 3879 4228 4274 4406 4449 4497
Size: 224 33 37 349 46 132 43 48
Cuts at: 4497 4506 4835 5012 5036 5778 5863 6934 1391
Size: 9 329 177 24 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 637 430 399 349 329
268 261 224 177 132 128 85 48
46 44 43 37 33 27 24 9
BspEI T'CCGG_A
Cuts at: 7380 7380
Size: 7717
BspHI T'CATG_A
Cuts at: 5606 7336 5606
Size: 1730 5987
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2139 3157 4081 5072 844
Size: 1295 1018 924 991 3489
Fragments arranged by size:
3489 1295 1018 991 924
BsrBI CCG'CTC
Cuts at: 1394 1823 2935 3420 3663 5604 1394
Size: 429 1112 485 243 1941 3507
Fragments arranged by size:
3507 1941 1112 485 429 243
BsrDI GCAATG_nn'
Cuts at: 65 1248 2629 3737 4096 6212 6386 65
Size: 1183 1381 1108 359 2116 174 1396
Fragments arranged by size:
2116 1396 1381 1183 1108 359 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6361 6548 1700
Size: 110 4551 187 2869
Fragments arranged by size:
4551 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3456 96
Size: 3360 4357
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7717
BssSI C'ACGA_G
Cuts at: 4499 5777 6789 4499
Size: 1278 1012 5427
Fragments arranged by size:
5427 1278 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2701 794
Size: 1907 5810
BstBI TT'CG_AA
Cuts at: 6521 6521
Size: 7717
BstEII G'GTnAC_C
Cuts at: 1184 2311 3709 3796 4660 1184
Size: 1127 1398 87 864 4241
Fragments arranged by size:
4241 1398 1127 864 87
BstXI CCAn_nnnn'nTGG
Cuts at: 4396 4396
Size: 7717
BstYI r'GATC_y
Cuts at: 1972 3516 3696 4521 5047 5414 5799 5816 7268
Size: 1544 180 825 526 367 385 17 1452
Cuts at: 7268 7354 7366 7712 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1544 1452 825 526 385 367 346
180 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2756 2756
Size: 7717
BtgI C'CryG_G
Cuts at: 513 1065 2004 2940 3278 3921 4062 513
Size: 552 939 936 338 643 141 4168
Fragments arranged by size:
4168 939 936 643 552 338 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2245 4954 487
Size: 683 56 1019 2709 3250
Fragments arranged by size:
3250 2709 1019 683 56
BtsI GCAGTG_nn'
Cuts at: 4274 5233 6027 6047 4274
Size: 959 794 20 5944
Fragments arranged by size:
5944 959 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7717
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7682
DraI TTT'AAA
Cuts at: 5021 5021
Size: 7717
DraIII CAC_nnn'GTG
Cuts at: 4165 4165
Size: 7717
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6724 809
Size: 507 5408 1802
Fragments arranged by size:
5408 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2490 2646 3468
Size: 54 1425 50 275 18 660 156 822
Cuts at: 3468 4059 5164 6053 7682 8
Size: 591 1105 889 1629 43
Fragments arranged by size:
1629 1425 1105 889 822 660 591 275
156 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5164 1537
Size: 275 3352 4090
Fragments arranged by size:
4090 3352 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2970 3002 5647 1353
Size: 1617 32 2645 3423
Fragments arranged by size:
3423 2645 1617 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2864 3694 3830 4949 6304 6678
Size: 1064 138 1275 830 136 1119 1355 374
Cuts at: 6678 6824 387
Size: 146 1280
Fragments arranged by size:
1355 1280 1275 1119 1064 830 374 146
138 136
EcoICRI GAG'CTC
Cuts at: 3583 3583
Size: 7717
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2255 2291 2300 2323 2543 2606 2711
Size: 393 623 36 9 23 220 63 105
Cuts at: 2711 2825 2885 3406 3533 3764 3917 3932 3983
Size: 114 60 521 127 231 153 15 51
Cuts at: 3983 4028 4204 4390 4624 4874 4948 5780 6377
Size: 45 176 186 234 250 74 832 597
Cuts at: 6377 7164 7432 1239
Size: 787 268 1524
Fragments arranged by size:
1524 832 787 623 597 521 393 268
250 234 231 220 186 176 153 127
114 105 74 63 60 51 45 36
23 15 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2135 2411 2672 2807 2879 3704 3914 4250 4369
Size: 276 261 135 72 825 210 336 119
Cuts at: 4369 2135
Size: 5483
Fragments arranged by size:
5483 825 336 276 261 210 135 119
72
EcoO109I rG'GnC_Cy
Cuts at: 2509 4003 4108 4816 2509
Size: 1494 105 708 5410
Fragments arranged by size:
5410 1494 708 105
EcoRI G'AATT_C
Cuts at: 5026 5404 5026
Size: 378 7339
FspI TGC'GCA
Cuts at: 3203 6223 3203
Size: 3020 4697
HaeII r_GCGC'y
Cuts at: 1678 2205 4895 6864 1678
Size: 527 2690 1969 2531
Fragments arranged by size:
2690 2531 1969 527
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2762 2794 4605 4637 6358
Size: 32 135 32 1190 32 1811 32 1721
Cuts at: 6358 6390 6432 6464 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1811 1721 1190 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2335 3973 4054 4786 5061 5307
Size: 988 281 397 1638 81 732 275 246
Cuts at: 5307 669
Size: 3079
Fragments arranged by size:
3079 1638 988 732 397 281 275 246
81
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7717
HpaI GTT'AAC
Cuts at: 5307 5307
Size: 7717
KasI G'GCGC_C
Cuts at: 4891 4891
Size: 7717
KpnI G_GTAC'C
Cuts at: 5042 5042
Size: 7717
MfeI C'AATT_G
Cuts at: 5316 5316
Size: 7717
MluI A'CGCG_T
Cuts at: 7593 7593
Size: 7717
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3354 4362 6831 7015 1305
Size: 2049 1008 2469 184 2007
Fragments arranged by size:
2469 2049 2007 1008 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4061 10
Size: 54 1425 343 2229 3666
Fragments arranged by size:
3666 2229 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2568 4442 5675 6034 6193 518
Size: 648 1402 1874 1233 359 159 2042
Fragments arranged by size:
2042 1874 1402 1233 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2158 2470 2521 3220 3292 3507 3610 4653
Size: 552 312 51 699 72 215 103 1043
Cuts at: 4653 4723 4825 4953 5133 5810 6958 7203 1606
Size: 70 102 128 180 677 1148 245 2120
Fragments arranged by size:
2120 1148 1043 699 677 552 312 245
215 180 128 103 102 72 70 51
NarI GG'CG_CC
Cuts at: 4892 4892
Size: 7717
NcoI C'CATG_G
Cuts at: 513 1065 2004 4062 513
Size: 552 939 2058 4168
Fragments arranged by size:
4168 2058 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7717
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7717
NotI GC'GGCC_GC
Cuts at: 5164 5164
Size: 7717
NsiI A_TGCA'T
Cuts at: 3309 3739 3309
Size: 430 7287
NspI r_CATG'y
Cuts at: 1132 1229 3311 4157 5075 6620 1132
Size: 97 2082 846 918 1545 2229
Fragments arranged by size:
2229 2082 1545 918 846 97
PasI CC'CwG_GG
Cuts at: 1348 4506 1348
Size: 3158 4559
PciI A'CATG_T
Cuts at: 6616 6616
Size: 7717
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3139 4006 4295 4675 1108
Size: 2031 867 289 380 4150
Fragments arranged by size:
4150 2031 867 380 289
PfoI T'CCnGG_A
Cuts at: 2268 3519 3894 3999 4251 4626 4710 4851 2268
Size: 1251 375 105 252 375 84 141 5134
Fragments arranged by size:
5134 1251 375 375 252 141 105 84
PmeI GTTT'AAAC
Cuts at: 5021 5021
Size: 7717
PmlI CAC'GTG
Cuts at: 4413 4413
Size: 7717
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3866 3898 5786 5818 7299 7331 3866
Size: 32 1888 32 1481 32 4252
Fragments arranged by size:
4252 1888 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4003 4108 4003
Size: 105 7612
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7717
PsiI TTA'TAA
Cuts at: 3987 5287 3987
Size: 1300 6417
PspXI vC'TCGA_Gb
Cuts at: 2241 2241
Size: 7717
PstI C_TGCA'G
Cuts at: 830 2325 2412 2475 2634 2673 2706 3000 3057
Size: 1495 87 63 159 39 33 294 57
Cuts at: 3057 3078 3225 3341 3627 4092 5069 830
Size: 21 147 116 286 465 977 3478
Fragments arranged by size:
3478 1495 977 465 294 286 159 147
116 87 63 57 39 33 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7717
PvuII CAG'CTG
Cuts at: 2158 2470 2521 3220 3507 3610 4723 4953 2158
Size: 312 51 699 287 103 1113 230 4922
Fragments arranged by size:
4922 1113 699 312 287 230 103 51
SacI G_AGCT'C
Cuts at: 3585 3585
Size: 7717
SalI G'TCGA_C
Cuts at: 1936 5059 1936
Size: 3123 4594
SbfI CC_TGCA'GG
Cuts at: 2412 2673 5069 2412
Size: 261 2396 5060
Fragments arranged by size:
5060 2396 261
ScaI AGT'ACT
Cuts at: 2969 4577 5965 7677 2969
Size: 1608 1388 1712 3009
Fragments arranged by size:
3009 1712 1608 1388
SexAI A'CCwGG_T
Cuts at: 3186 3186
Size: 7717
SfcI C'TryA_G
Cuts at: 826 1045 2321 2408 2471 2617 2630 2669 2702
Size: 219 1276 87 63 146 13 39 33
Cuts at: 2702 2996 3031 3053 3074 3221 3337 3623 4088
Size: 294 35 22 21 147 116 286 465
Cuts at: 4088 4820 5065 6200 6881 7072 826
Size: 732 245 1135 681 191 1471
Fragments arranged by size:
1471 1276 1135 732 681 465 294 286
245 219 191 147 146 116 87 63
39 35 33 22 21 13
SfoI GGC'GCC
Cuts at: 4893 4893
Size: 7717
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7717
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7717
SmaI CCC'GGG
Cuts at: 1872 3534 3804 3837 5044 1872
Size: 1662 270 33 1207 4545
Fragments arranged by size:
4545 1662 1207 270 33
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2241 3245 4579 4808 5821 6722
Size: 197 926 298 1004 1334 229 1013 901
Cuts at: 6722 6984 820
Size: 262 1553
Fragments arranged by size:
1553 1334 1013 1004 926 901 298 262
229 197
SnaBI TAC'GTA
Cuts at: 493 5508 493
Size: 5015 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7717
SphI G_CATG'C
Cuts at: 1132 3311 4157 5075 1132
Size: 2179 846 918 3774
Fragments arranged by size:
3774 2179 918 846
SrfI GCCC'GGGC
Cuts at: 3837 3837
Size: 7717
SspI AAT'ATT
Cuts at: 5 52 5641 5
Size: 47 5589 2081
Fragments arranged by size:
5589 2081 47
StuI AGG'CCT
Cuts at: 1517 4601 1517
Size: 3084 4633
StyI C'CwwG_G
Cuts at: 513 1065 2004 2142 4062 4341 4549 5156 513
Size: 552 939 138 1920 279 208 607 3074
Fragments arranged by size:
3074 1920 939 607 552 279 208 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2393 3857 4648 4715 5744 5761 5914
Size: 1255 813 1464 791 67 1029 17 153
Cuts at: 5914 6099 6544 325
Size: 185 445 1498
Fragments arranged by size:
1498 1464 1255 1029 813 791 445 185
153 67 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2967 3456 4575
Size: 274 80 33 51 1443 990 489 1119
Cuts at: 4575 5963 7675 96
Size: 1388 1712 138
Fragments arranged by size:
1712 1443 1388 1119 990 489 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2045 2077 7677 7709 2045
Size: 32 5600 32 2053
Fragments arranged by size:
5600 2053 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3188 4730 1316
Size: 1872 1542 4303
Fragments arranged by size:
4303 1872 1542
XbaI T'CTAG_A
Cuts at: 5053 5053
Size: 7717
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2266 3400 2266
Size: 1134 6583
XhoI C'TCGA_G
Cuts at: 1943 2241 1943
Size: 298 7419
XmaI C'CCGG_G
Cuts at: 1870 3532 3802 3835 5042 1870
Size: 1662 270 33 1207 4545
Fragments arranged by size:
4545 1662 1207 270 33
XmnI GAAnn'nnTTC
Cuts at: 5846 5846
Size: 7717
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2299 4717 5525 276
Size: 53 83 186 936 765 2418 808 2468
Fragments arranged by size:
2468 2418 936 808 765 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BstZ17I EcoRV
FalI FseI FspAI NaeI NgoMIV NruI PacI PspOMI
PsrI RsrII SacII SanDI SapI SfiI SwaI