################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## Acc65I AclI AcsI AflII AgeI ApoI AscI AseI AsnI Asp700I Asp718I AviII AvrII BbvCI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsiEI BsiWI BsmI BspDI BspHI BspLU11I BsrDI BssHII Bst1107I BstAPI BstBI BstXI BstZ17I CelII ClaI CpoI DraI DraIII EagI EclXI Eco52I EcoNI EcoRI EcoRV FbaI FseI FspI HindIII HpaI KpnI MaeI MamI MfeI MluI MseI MunI NcoI NdeI NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SbfI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspI SwaI TfiI Tru9I Tsp509I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AatII 1963 GACGTC GACGT^C AccI 561 GTCGAC GT^[AC][GT]AC AflIII 1040 ACGTGT A^C[GA][CT]GT AocI 2244 CCTGAGG CC^T[ACGT]AGG Aor51HI 1869 AGCGCT AGC^GCT AspI 1471 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 1611 GGCGCC GGCGC^C BbrPI 1759 CACGTG CAC^GTG BbsI 1513 GAAGAC GAAGAC(2/6) BpmI 874 CTGGAG CTGGAG(16/14) BpuAI 1513 GAAGAC GAAGAC(2/6) BsaAI 1759 CACGTG [CT]AC^GT[GA] BsaI 1782 GGTCTC GGTCTC(1/5) BsrBI 1348 GAGCGG CCGCTC(-3/-3) BssSI 1330 CACGAG CACGAG(-5/-1) BstEII 181 GGTTACC G^GT[ACGT]ACC BstPI 181 GGTTACC G^GT[ACGT]ACC Bsu36I 2244 CCTGAGG CC^T[ACGT]AGG DrdI 1084 GACCAGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1813 CTCTTC CTCTTC(1/4) Eco47III 1869 AGCGCT AGC^GCT Eco81I 2244 CCTGAGG CC^T[ACGT]AGG EcoO65I 181 GGTTACC G^GT[ACGT]ACC EcoT14I 1590 CCAAGG C^C[AT][AT]GG EcoT22I 1110 ATGCAT ATGCA^T HincII 561 GTCGAC GT[CT]^[GA]AC HindII 561 GTCGAC GT[CT]^[GA]AC KasI 1611 GGCGCC G^GCGCC Ksp632I 1813 CTCTTC CTCTTC(1/4) NarI 1611 GGCGCC GG^CGCC NsiI 1110 ATGCAT ATGCA^T PflFI 1471 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1773 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1759 CACGTG CAC^GTG PmlI 1759 CACGTG CAC^GTG SalI 561 GTCGAC G^TCGAC SapI 1812 GCTCTTC GCTCTTC(1/4) ScaI 1382 AGTACT AGT^ACT SfoI 1611 GGCGCC GGC^GCC SmaI 1700 CCCGGG CCC^GGG StyI 1590 CCAAGG C^C[AT][AT]GG Tth111I 1471 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1773 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 1700 CCCGGG C^CCGGG XmaI 1700 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccIII 1478 TCCGGA T^CCGGA 2048 TCCGGA AhdI 564 GACGGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1027 GACAAGCTGTC Alw44I 136 GTGCAC G^TGCAC 2172 GTGCAC ApaI 1895 GGGCCC GGGCC^C 2262 GGGCCC ApaLI 136 GTGCAC G^TGCAC 2172 GTGCAC AspEI 564 GACGGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1027 GACAAGCTGTC BamHI 1544 GGATCC G^GATCC 2093 GGATCC BcgI 380 CGACGGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2057 CGAGGAGTTTGC BsaWI 1478 TCCGGA [AT]^CCGG[AT] 2048 TCCGGA BseAI 1478 TCCGGA T^CCGGA 2048 TCCGGA BsmBI 1191 CGTCTC CGTCTC(1/5) 1153 GAGACG BspEI 1478 TCCGGA T^CCGGA 2048 TCCGGA BspMI 1733 ACCTGC ACCTGC(4/8) 1089 GCAGGT Eam1105I 564 GACGGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1027 GACAAGCTGTC MroI 1478 TCCGGA T^CCGGA 2048 TCCGGA NaeI 338 GCCGGC GCC^GGC 799 GCCGGC NgoMIV 338 GCCGGC G^CCGGC 799 GCCGGC PvuII 964 CAGCTG CAG^CTG 1978 CAGCTG SexAI 1604 ACCTGGT A^CC[AT]GGT 1778 ACCTGGT SfiI 1887 GGCCTGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 1938 GGCCATCGTGGCC SphI 452 GCATGC GCATG^C 1112 GCATGC StuI 947 AGGCCT AGG^CCT 1886 AGGCCT TaqI 562 TCGA T^CGA 1970 TCGA Tsp45I 583 GTCAC ^GT[GC]AC 770 GTGAC TspRI 1051 CAGTG CA[GC]TG[ACGT][ACGT]^ 1458 CACTG TthHB8I 562 TCGA T^CGA 1970 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BglI BmyI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BsrFI BsrGI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KspI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI RsaI SacI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SspBI TseI XcmI XhoII