(Circular) (Six-base) MAPSORT of: pFN21AE4221.seq Check: 5504 from: 1 to: 7006 pFN21AE4221 7006 bp With 182 enzymes: SgfI * March 28, 2012 13:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3972 4816 278 Size: 53 83 186 936 2436 844 2468 Fragments arranged by size: 2468 2436 936 844 186 83 53 AccI GT'mk_AC Cuts at: 1937 2567 4349 1937 Size: 630 1782 4594 Fragments arranged by size: 4594 1782 630 Acc65I G'GTAC_C Cuts at: 4327 4327 Size: 7006 AclI AA'CG_TT Cuts at: 1563 5133 5506 1563 Size: 3570 373 3063 Fragments arranged by size: 3570 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2926 5069 6453 6721 1632 Size: 1294 2143 1384 268 1917 Fragments arranged by size: 2143 1917 1384 1294 268 AfeI AGC'GCT Cuts at: 3876 3876 Size: 7006 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6809 AflIII A'CryG_T Cuts at: 1203 3045 5905 6882 1203 Size: 1842 2860 977 1327 Fragments arranged by size: 2860 1842 1327 977 AgeI A'CCGG_T Cuts at: 5837 5837 Size: 7006 AhdI GACnn_n'nnGTC Cuts at: 2574 3037 5735 2574 Size: 463 2698 3845 Fragments arranged by size: 3845 2698 463 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7006 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3092 1184 Size: 1908 5098 AlwNI CAG_nnn'CTG Cuts at: 2167 3784 3985 4749 6321 2167 Size: 1617 201 764 1572 2852 Fragments arranged by size: 2852 1617 1572 764 201 ApaI G_GGCC'C Cuts at: 3904 4271 3904 Size: 367 6639 ApaLI G'TGCA_C Cuts at: 2141 4177 5063 6219 2141 Size: 2036 886 1156 2928 Fragments arranged by size: 2928 2036 1156 886 ApoI r'AATT_y Cuts at: 1493 4315 4541 4693 6802 1493 Size: 2822 226 152 2109 1697 Fragments arranged by size: 2822 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5560 160 Size: 872 4528 1606 Fragments arranged by size: 4528 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7006 AvaI C'yCGr_G Cuts at: 1870 1943 2303 2337 2615 3167 3425 3705 3863 Size: 73 360 34 278 552 258 280 158 Cuts at: 3863 4331 1870 Size: 468 4545 Fragments arranged by size: 4545 552 468 360 280 278 258 158 73 34 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3719 3752 3719 Size: 33 6973 BamHI G'GATC_C Cuts at: 3549 4098 4336 4703 3549 Size: 549 238 367 5852 Fragments arranged by size: 5852 549 367 238 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2326 2519 3616 4327 5782 Size: 325 217 627 539 193 1097 711 1455 Cuts at: 5782 618 Size: 1842 Fragments arranged by size: 1842 1455 1097 711 627 539 325 217 193 BanII G_rGCy'C Cuts at: 1391 2312 2360 3904 4047 4271 4325 1391 Size: 921 48 1544 143 224 54 4072 Fragments arranged by size: 4072 1544 921 224 143 54 48 BbeI G_GCGC'C Cuts at: 3620 3620 Size: 7006 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3525 928 Size: 971 1626 4409 Fragments arranged by size: 4409 1626 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2374 2408 4051 4085 5197 5231 1903 Size: 34 437 34 1643 34 1112 34 3678 Fragments arranged by size: 3678 1643 1112 437 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4898 6108 4898 Size: 1210 5796 BclI T'GATC_A Cuts at: 1548 1548 Size: 7006 BfrBI ATG'CAT Cuts at: 3117 3117 Size: 7006 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2155 2340 3899 Size: 107 122 71 631 605 483 185 1559 Cuts at: 3899 3950 5617 136 Size: 51 1667 1525 Fragments arranged by size: 1667 1559 1525 631 605 483 185 122 107 71 51 BglII A'GATC_T Cuts at: 7001 7001 Size: 7006 BlpI GC'TnA_GC Cuts at: 4423 4423 Size: 7006 Bme1580I G_kGCm'C Cuts at: 1790 2145 2524 3904 4181 4223 4271 5067 6223 Size: 355 379 1380 277 42 48 796 1156 Cuts at: 6223 1790 Size: 2573 Fragments arranged by size: 2573 1380 1156 796 379 355 277 48 42 BmgBI CAC'GTC Cuts at: 1318 3046 3345 3478 1318 Size: 1728 299 133 4846 Fragments arranged by size: 4846 1728 299 133 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4737 5695 442 Size: 416 53 83 399 24 3320 958 1753 Fragments arranged by size: 3320 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7006 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2249 2653 2249 Size: 404 6602 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2900 5666 1239 Size: 1661 2766 2579 Fragments arranged by size: 2766 2579 1661 Bpu10I CC'TnA_GC Cuts at: 2359 2359 Size: 7006 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5131 5996 6294 5131 Size: 865 298 5843 Fragments arranged by size: 5843 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3793 5669 882 Size: 617 2294 1876 2219 Fragments arranged by size: 2294 2219 1876 617 BsaAI yAC'GTr Cuts at: 493 1204 3766 4797 493 Size: 711 2562 1031 2702 Fragments arranged by size: 2702 2562 1031 711 BsaBI GATnn'nnATC Cuts at: 7000 7000 Size: 7006 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2802 3195 3617 3969 Size: 53 83 186 936 1269 393 422 352 Cuts at: 3969 4277 4813 5195 5830 275 Size: 308 536 382 635 1451 Fragments arranged by size: 1451 1269 936 635 536 422 393 382 352 308 186 83 53 BsaWI w'CCGG_w Cuts at: 3483 4053 5439 5837 6111 6258 6669 3483 Size: 570 1386 398 274 147 411 3820 Fragments arranged by size: 3820 1386 570 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2363 2393 2687 2717 2909 Size: 30 165 30 960 30 294 30 192 Cuts at: 2909 2939 3164 3194 3230 3260 4142 4172 1178 Size: 30 225 30 36 30 882 30 4012 Fragments arranged by size: 4012 960 882 294 225 192 165 36 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2026 2294 2501 2609 2642 2695 3185 3254 Size: 840 268 207 108 33 53 490 69 Cuts at: 3254 3257 3365 3443 3559 4078 1186 Size: 3 108 78 116 519 4114 Fragments arranged by size: 4114 840 519 490 268 207 116 108 108 78 69 53 33 3 BseYI C'CCAG_C Cuts at: 2167 2221 2523 2850 3226 3793 3897 6209 6705 Size: 54 302 327 376 567 104 2312 496 Cuts at: 6705 2167 Size: 2468 Fragments arranged by size: 2468 2312 567 496 376 327 302 104 54 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2941 3052 3476 3992 4097 1163 Size: 573 1205 111 424 516 105 4072 Fragments arranged by size: 4072 1205 573 516 424 111 105 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4456 5217 6245 1540 Size: 275 186 2455 761 1028 2301 Fragments arranged by size: 2455 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2145 2178 2312 2360 3435 4047 4181 5067 5152 Size: 33 134 48 1075 612 134 886 85 Cuts at: 5152 6223 2145 Size: 1071 2928 Fragments arranged by size: 2928 1075 1071 886 612 134 134 85 48 33 BsmI GAATG_Cn' Cuts at: 4517 4610 4517 Size: 93 6913 BsmBI CGTCTCn'nnnn_ Cuts at: 3152 3202 3152 Size: 50 6956 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2145 2178 2312 2360 2524 3435 3904 Size: 399 355 33 134 48 164 911 469 Cuts at: 3904 4047 4181 4223 4271 4325 5067 5152 6223 Size: 143 134 42 48 54 742 85 1071 Cuts at: 6223 1391 Size: 2174 Fragments arranged by size: 2174 1071 911 742 469 399 355 164 143 134 134 85 54 48 48 42 33 BspEI T'CCGG_A Cuts at: 3483 4053 6669 3483 Size: 570 2616 3820 Fragments arranged by size: 3820 2616 570 BspHI T'CATG_A Cuts at: 4895 6625 4895 Size: 1730 5276 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3085 3747 4361 844 Size: 2241 662 614 3489 Fragments arranged by size: 3489 2241 662 614 BsrBI CCG'CTC Cuts at: 1394 1823 3355 4893 1394 Size: 429 1532 1538 3507 Fragments arranged by size: 3507 1538 1532 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5501 5675 65 Size: 1183 4253 174 1396 Fragments arranged by size: 4253 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2343 2804 4181 5650 5837 1700 Size: 110 533 461 1377 1469 187 2869 Fragments arranged by size: 2869 1469 1377 533 461 187 110 BsrGI T'GTAC_A Cuts at: 96 2199 3180 3570 96 Size: 2103 981 390 3532 Fragments arranged by size: 3532 2103 981 390 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7006 BssSI C'ACGA_G Cuts at: 3335 5066 6078 3335 Size: 1731 1012 4263 Fragments arranged by size: 4263 1731 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7006 BstBI TT'CG_AA Cuts at: 5810 5810 Size: 7006 BstEII G'GTnAC_C Cuts at: 1184 2186 1184 Size: 1002 6004 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 7006 BstYI r'GATC_y Cuts at: 1972 2559 3396 3549 3882 4098 4336 4703 5088 Size: 587 837 153 333 216 238 367 385 Cuts at: 5088 5105 6557 6643 6655 7001 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 837 587 385 367 346 333 238 216 153 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4250 4250 Size: 7006 BtgI C'CryG_G Cuts at: 513 1065 2609 2752 3019 3295 3990 4167 513 Size: 552 1544 143 267 276 695 177 3352 Fragments arranged by size: 3352 1544 695 552 276 267 177 143 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6267 Fragments arranged by size: 6267 683 56 BtsI GCAGTG_nn' Cuts at: 3062 4522 5316 5336 3062 Size: 1460 794 20 4732 Fragments arranged by size: 4732 1460 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7006 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6971 DraI TTT'AAA Cuts at: 4310 4310 Size: 7006 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3095 6013 809 Size: 507 1779 2918 1802 Fragments arranged by size: 2918 1802 1779 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2742 2965 3627 Size: 54 1425 50 275 18 912 223 662 Cuts at: 3627 3942 3951 3987 4023 4183 4453 5342 6971 Size: 315 9 36 36 160 270 889 1629 Cuts at: 6971 8 Size: 43 Fragments arranged by size: 1629 1425 912 889 662 315 275 270 223 160 54 50 43 36 36 18 9 EagI C'GGCC_G Cuts at: 1537 1812 4453 1537 Size: 275 2641 4090 Fragments arranged by size: 4090 2641 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3824 4936 1353 Size: 2471 1112 3423 Fragments arranged by size: 3423 2471 1112 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2921 3122 3190 4073 5593 5967 Size: 1064 138 1332 201 68 883 1520 374 Cuts at: 5967 6113 387 Size: 146 1280 Fragments arranged by size: 1520 1332 1280 1064 883 374 201 146 138 68 EcoICRI GAG'CTC Cuts at: 2310 2358 4045 2310 Size: 48 1687 5271 Fragments arranged by size: 5271 1687 48 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2900 2926 5069 5666 6453 6721 1239 Size: 393 1268 26 2143 597 787 268 1524 Fragments arranged by size: 2143 1524 1268 787 597 393 268 26 EcoO109I rG'GnC_Cy Cuts at: 2031 2088 2248 2284 3634 4267 2031 Size: 57 160 36 1350 633 4770 Fragments arranged by size: 4770 1350 633 160 57 36 EcoRI G'AATT_C Cuts at: 4315 4693 4315 Size: 378 6628 FspI TGC'GCA Cuts at: 5512 5512 Size: 7006 HaeII r_GCGC'y Cuts at: 1678 2741 3365 3620 3878 6153 1678 Size: 1063 624 255 258 2275 2531 Fragments arranged by size: 2531 2275 1063 624 258 255 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2100 2132 2560 2592 2675 Size: 32 135 32 528 32 428 32 83 Cuts at: 2675 2687 2707 2719 3023 3055 3329 3361 4142 Size: 12 20 12 304 32 274 32 781 Cuts at: 4142 4174 5647 5679 5721 5753 1373 Size: 32 1473 32 42 32 2626 Fragments arranged by size: 2626 1473 781 528 428 304 274 135 83 42 32 32 32 32 32 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 2568 4350 4596 669 Size: 988 281 630 1782 246 3079 Fragments arranged by size: 3079 1782 988 630 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7006 HpaI GTT'AAC Cuts at: 4596 4596 Size: 7006 KasI G'GCGC_C Cuts at: 3616 3616 Size: 7006 KpnI G_GTAC'C Cuts at: 4331 4331 Size: 7006 MfeI C'AATT_G Cuts at: 4605 4605 Size: 7006 MluI A'CGCG_T Cuts at: 6882 6882 Size: 7006 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2823 3327 3585 6120 6304 1305 Size: 1518 504 258 2535 184 2007 Fragments arranged by size: 2535 2007 1518 504 258 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3944 3953 3989 4025 10 Size: 54 1425 343 2112 9 36 36 2991 Fragments arranged by size: 2991 2112 1425 343 54 36 36 9 MslI CAynn'nnrTG Cuts at: 518 1166 2429 3074 3219 3938 4031 4964 5323 Size: 648 1263 645 145 719 93 933 359 Cuts at: 5323 5482 518 Size: 159 2042 Fragments arranged by size: 2042 1263 933 719 648 645 359 159 145 93 MspA1I CmG'CkG Cuts at: 1606 2079 2085 2611 2754 2866 2964 2971 3297 Size: 473 6 526 143 112 98 7 326 Cuts at: 3297 3831 3985 4155 4169 4422 5099 6247 6492 Size: 534 154 170 14 253 677 1148 245 Cuts at: 6492 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 534 526 473 326 253 245 170 154 143 112 98 14 7 6 NaeI GCC'GGC Cuts at: 2345 2806 2345 Size: 461 6545 NarI GG'CG_CC Cuts at: 3617 3617 Size: 7006 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6454 NdeI CA'TA_TG Cuts at: 387 387 Size: 7006 NgoMIV G'CCGG_C Cuts at: 2343 2804 2343 Size: 461 6545 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7006 NotI GC'GGCC_GC Cuts at: 4453 4453 Size: 7006 NsiI A_TGCA'T Cuts at: 3119 3119 Size: 7006 NspI r_CATG'y Cuts at: 1132 1229 2461 2923 3117 3121 3181 4147 4364 Size: 97 1232 462 194 4 60 966 217 Cuts at: 4364 5909 1132 Size: 1545 2229 Fragments arranged by size: 2229 1545 1232 966 462 217 194 97 60 4 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7006 PciI A'CATG_T Cuts at: 5905 5905 Size: 7006 PflMI CCAn_nnn'nTGG Cuts at: 1108 3784 1108 Size: 2676 4330 PfoI T'CCnGG_A Cuts at: 2100 2555 2817 3636 2100 Size: 455 262 819 5470 Fragments arranged by size: 5470 819 455 262 PmeI GTTT'AAAC Cuts at: 4310 4310 Size: 7006 PmlI CAC'GTG Cuts at: 3766 3766 Size: 7006 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5075 5107 6588 6620 5075 Size: 32 1481 32 5461 Fragments arranged by size: 5461 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2088 2248 3634 2088 Size: 160 1386 5460 Fragments arranged by size: 5460 1386 160 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7006 PsiI TTA'TAA Cuts at: 4576 4576 Size: 7006 PspOMI G'GGCC_C Cuts at: 3900 4267 3900 Size: 367 6639 PstI C_TGCA'G Cuts at: 830 2163 2841 3375 3813 4109 4358 830 Size: 1333 678 534 438 296 249 3478 Fragments arranged by size: 3478 1333 678 534 438 296 249 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7006 PvuII CAG'CTG Cuts at: 2971 3985 2971 Size: 1014 5992 SacI G_AGCT'C Cuts at: 2312 2360 4047 2312 Size: 48 1687 5271 Fragments arranged by size: 5271 1687 48 SacII CC_GC'GG Cuts at: 2612 2755 3298 4170 2612 Size: 143 543 872 5448 Fragments arranged by size: 5448 872 543 143 SalI G'TCGA_C Cuts at: 1936 2566 4348 1936 Size: 630 1782 4594 Fragments arranged by size: 4594 1782 630 SanDI GG'GwC_CC Cuts at: 2088 2248 2088 Size: 160 6846 SapI GCTCTTCn'nnn_ Cuts at: 3824 3824 Size: 7006 SbfI CC_TGCA'GG Cuts at: 4358 4358 Size: 7006 ScaI AGT'ACT Cuts at: 3389 5254 6966 3389 Size: 1865 1712 3429 Fragments arranged by size: 3429 1865 1712 SexAI A'CCwGG_T Cuts at: 3609 3783 3609 Size: 174 6832 SfcI C'TryA_G Cuts at: 826 1045 2159 2837 3371 3809 4105 4354 5489 Size: 219 1114 678 534 438 296 249 1135 Cuts at: 5489 6170 6361 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1114 681 678 534 438 296 249 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3899 3950 3899 Size: 51 6955 SfoI GGC'GCC Cuts at: 3618 3618 Size: 7006 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7006 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7006 SmaI CCC'GGG Cuts at: 1872 3707 4333 1872 Size: 1835 626 4545 Fragments arranged by size: 4545 1835 626 SmlI C'TyrA_G Cuts at: 820 1017 1943 5110 6011 6273 820 Size: 197 926 3167 901 262 1553 Fragments arranged by size: 3167 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4797 493 Size: 4304 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7006 SphI G_CATG'C Cuts at: 1132 2461 3121 4364 1132 Size: 1329 660 1243 3774 Fragments arranged by size: 3774 1329 1243 660 SspI AAT'ATT Cuts at: 5 52 4930 5 Size: 47 4878 2081 Fragments arranged by size: 4878 2081 47 StuI AGG'CCT Cuts at: 1517 2954 3893 1517 Size: 1437 939 4630 Fragments arranged by size: 4630 1437 939 StyI C'CwwG_G Cuts at: 513 1065 3595 4445 513 Size: 552 2530 850 3074 Fragments arranged by size: 3074 2530 850 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2196 5033 5050 5203 5388 5833 325 Size: 1255 616 2837 17 153 185 445 1498 Fragments arranged by size: 2837 1498 1255 616 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2199 2997 3180 Size: 274 80 33 51 1443 222 798 183 Cuts at: 3180 3246 3387 3570 5252 6964 96 Size: 66 141 183 1682 1712 138 Fragments arranged by size: 1712 1682 1443 798 274 222 183 183 141 138 80 66 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2363 2395 2909 2941 6966 6998 2363 Size: 32 514 32 4025 32 2371 Fragments arranged by size: 4025 2371 514 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3479 1316 Size: 2163 4843 XbaI T'CTAG_A Cuts at: 4342 4342 Size: 7006 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2236 2968 4168 2236 Size: 732 1200 5074 Fragments arranged by size: 5074 1200 732 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7006 XmaI C'CCGG_G Cuts at: 1870 3705 4331 1870 Size: 1835 626 4545 Fragments arranged by size: 4545 1835 626 XmnI GAAnn'nnTTC Cuts at: 5135 5135 Size: 7006 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3970 4814 276 Size: 53 83 186 936 2436 844 2468 Fragments arranged by size: 2468 2436 936 844 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BbvCI BsiWI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NruI PacI PspXI PsrI RsrII SrfI SwaI