(Linear) (Six-base) MAPSORT of: KIEE4221.seq Check: 6241 from: 1 to: 2301 KIEE4221.orf With 182 enzymes: SgfI * March 22, 2012 17:35 .. AatII G_ACGT'C Cuts at: 0 1967 2301 Size: 1967 334 AccI GT'mk_AC Cuts at: 0 562 2301 Size: 562 1739 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 921 2301 Size: 921 1380 AfeI AGC'GCT Cuts at: 0 1871 2301 Size: 1871 430 AflIII A'CryG_T Cuts at: 0 1040 2301 Size: 1040 1261 AhdI GACnn_n'nnGTC Cuts at: 0 569 1032 2301 Size: 569 463 1269 Fragments arranged by size: 1269 569 463 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1087 2301 Size: 1087 1214 AlwNI CAG_nnn'CTG Cuts at: 0 162 1779 1980 2301 Size: 162 1617 201 321 Fragments arranged by size: 1617 321 201 162 ApaI G_GGCC'C Cuts at: 0 1899 2266 2301 Size: 1899 367 35 Fragments arranged by size: 1899 367 35 ApaLI G'TGCA_C Cuts at: 0 136 2172 2301 Size: 136 2036 129 Fragments arranged by size: 2036 136 129 AvaI C'yCGr_G Cuts at: 0 298 332 610 1162 1420 1700 1858 2301 Size: 298 34 278 552 258 280 158 443 Fragments arranged by size: 552 443 298 280 278 258 158 34 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1714 1747 2301 Size: 1714 33 554 Fragments arranged by size: 1714 554 33 BamHI G'GATC_C Cuts at: 0 1544 2093 2301 Size: 1544 549 208 Fragments arranged by size: 1544 549 208 BanI G'GyrC_C Cuts at: 0 321 514 1611 2301 Size: 321 193 1097 690 Fragments arranged by size: 1097 690 321 193 BanII G_rGCy'C Cuts at: 0 307 355 1899 2042 2266 2301 Size: 307 48 1544 143 224 35 Fragments arranged by size: 1544 307 224 143 48 35 BbeI G_GCGC'C Cuts at: 0 1615 2301 Size: 1615 686 BbsI GAAGACnn'nnnn_ Cuts at: 0 1520 2301 Size: 1520 781 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 369 403 2046 2080 2301 Size: 369 34 1643 34 221 Fragments arranged by size: 1643 369 221 34 34 BfrBI ATG'CAT Cuts at: 0 1112 2301 Size: 1112 1189 BglI GCCn_nnn'nGGC Cuts at: 0 150 335 1894 1945 2301 Size: 150 185 1559 51 356 Fragments arranged by size: 1559 356 185 150 51 Bme1580I G_kGCm'C Cuts at: 0 140 519 1899 2176 2218 2266 2301 Size: 140 379 1380 277 42 48 35 Fragments arranged by size: 1380 379 277 140 48 42 35 BmgBI CAC'GTC Cuts at: 0 1041 1340 1473 2301 Size: 1041 299 133 828 Fragments arranged by size: 1041 828 299 133 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 244 648 2301 Size: 244 404 1653 Fragments arranged by size: 1653 404 244 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 895 2301 Size: 895 1406 Bpu10I CC'TnA_GC Cuts at: 0 354 2301 Size: 354 1947 BsaI GGTCTCn'nnnn_ Cuts at: 0 1788 2301 Size: 1788 513 BsaAI yAC'GTr Cuts at: 0 1761 2301 Size: 1761 540 BsaHI Gr'CG_yC Cuts at: 0 797 1190 1612 1964 2272 2301 Size: 797 393 422 352 308 29 Fragments arranged by size: 797 422 393 352 308 29 BsaWI w'CCGG_w Cuts at: 0 1478 2048 2301 Size: 1478 570 253 Fragments arranged by size: 1478 570 253 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 358 388 682 712 904 934 1159 1189 Size: 358 30 294 30 192 30 225 30 Cuts at: 1189 1225 1255 2137 2167 2301 Size: 36 30 882 30 134 Fragments arranged by size: 882 358 294 225 192 134 36 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 21 289 496 604 637 690 1180 1249 Size: 21 268 207 108 33 53 490 69 Cuts at: 1249 1252 1360 1438 1554 2073 2301 Size: 3 108 78 116 519 228 Fragments arranged by size: 519 490 268 228 207 116 108 108 78 69 53 33 21 3 BseYI C'CCAG_C Cuts at: 0 162 216 518 845 1221 1788 1892 2301 Size: 162 54 302 327 376 567 104 409 Fragments arranged by size: 567 409 376 327 302 162 104 54 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 936 1047 1471 1987 2092 2301 Size: 936 111 424 516 105 209 Fragments arranged by size: 936 516 424 209 111 105 BsiHKAI G_wGCw'C Cuts at: 0 140 173 307 355 1430 2042 2176 2301 Size: 140 33 134 48 1075 612 134 125 Fragments arranged by size: 1075 612 140 134 134 125 48 33 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1147 1197 2301 Size: 1147 50 1104 Fragments arranged by size: 1147 1104 50 Bsp1286I G_dGCh'C Cuts at: 0 140 173 307 355 519 1430 1899 2042 Size: 140 33 134 48 164 911 469 143 Cuts at: 2042 2176 2218 2266 2301 Size: 134 42 48 35 Fragments arranged by size: 911 469 164 143 140 134 134 48 48 42 35 33 BspEI T'CCGG_A Cuts at: 0 1478 2048 2301 Size: 1478 570 253 Fragments arranged by size: 1478 570 253 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1080 1742 2301 Size: 1080 662 559 Fragments arranged by size: 1080 662 559 BsrBI CCG'CTC Cuts at: 0 1350 2301 Size: 1350 951 BsrFI r'CCGG_y Cuts at: 0 338 799 2176 2301 Size: 338 461 1377 125 Fragments arranged by size: 1377 461 338 125 BsrGI T'GTAC_A Cuts at: 0 194 1175 1565 2301 Size: 194 981 390 736 Fragments arranged by size: 981 736 390 194 BssSI C'ACGA_G Cuts at: 0 1330 2301 Size: 1330 971 BstEII G'GTnAC_C Cuts at: 0 181 2301 Size: 181 2120 BstYI r'GATC_y Cuts at: 0 554 1391 1544 1877 2093 2301 Size: 554 837 153 333 216 208 Fragments arranged by size: 837 554 333 216 208 153 Bsu36I CC'TnA_GG Cuts at: 0 2245 2301 Size: 2245 56 BtgI C'CryG_G Cuts at: 0 604 747 1014 1290 1985 2162 2301 Size: 604 143 267 276 695 177 139 Fragments arranged by size: 695 604 276 267 177 143 139 BtsI GCAGTG_nn' Cuts at: 0 1057 2301 Size: 1057 1244 DrdI GACnn_nn'nnGTC Cuts at: 0 1090 2301 Size: 1090 1211 EaeI y'GGCC_r Cuts at: 0 737 960 1622 1937 1946 1982 2018 2178 Size: 737 223 662 315 9 36 36 160 Cuts at: 2178 2301 Size: 123 Fragments arranged by size: 737 662 315 223 160 123 36 36 9 EarI CTCTTCn'nnn_ Cuts at: 0 1819 2301 Size: 1819 482 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 916 1117 1185 2068 2301 Size: 916 201 68 883 233 Fragments arranged by size: 916 883 233 201 68 EcoICRI GAG'CTC Cuts at: 0 305 353 2040 2301 Size: 305 48 1687 261 Fragments arranged by size: 1687 305 261 48 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 895 921 2301 Size: 895 26 1380 Fragments arranged by size: 1380 895 26 EcoO109I rG'GnC_Cy Cuts at: 0 26 83 243 279 1629 2262 2301 Size: 26 57 160 36 1350 633 39 Fragments arranged by size: 1350 633 160 57 39 36 26 HaeII r_GCGC'y Cuts at: 0 736 1360 1615 1873 2301 Size: 736 624 255 258 428 Fragments arranged by size: 736 624 428 258 255 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 95 127 555 587 670 682 702 714 Size: 95 32 428 32 83 12 20 12 Cuts at: 714 1018 1050 1324 1356 2137 2169 2301 Size: 304 32 274 32 781 32 132 Fragments arranged by size: 781 428 304 274 132 95 83 32 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 0 563 2301 Size: 563 1738 KasI G'GCGC_C Cuts at: 0 1611 2301 Size: 1611 690 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 818 1322 1580 2301 Size: 818 504 258 721 Fragments arranged by size: 818 721 504 258 MscI TGG'CCA Cuts at: 0 1939 1948 1984 2020 2301 Size: 1939 9 36 36 281 Fragments arranged by size: 1939 281 36 36 9 MslI CAynn'nnrTG Cuts at: 0 424 1069 1214 1933 2026 2301 Size: 424 645 145 719 93 275 Fragments arranged by size: 719 645 424 275 145 93 MspA1I CmG'CkG Cuts at: 0 74 80 606 749 861 959 966 1292 Size: 74 6 526 143 112 98 7 326 Cuts at: 1292 1826 1980 2150 2164 2301 Size: 534 154 170 14 137 Fragments arranged by size: 534 526 326 170 154 143 137 112 98 74 14 7 6 NaeI GCC'GGC Cuts at: 0 340 801 2301 Size: 340 461 1500 Fragments arranged by size: 1500 461 340 NarI GG'CG_CC Cuts at: 0 1612 2301 Size: 1612 689 NgoMIV G'CCGG_C Cuts at: 0 338 799 2301 Size: 338 461 1502 Fragments arranged by size: 1502 461 338 NsiI A_TGCA'T Cuts at: 0 1114 2301 Size: 1114 1187 NspI r_CATG'y Cuts at: 0 456 918 1112 1116 1176 2142 2301 Size: 456 462 194 4 60 966 159 Fragments arranged by size: 966 462 456 194 159 60 4 PflMI CCAn_nnn'nTGG Cuts at: 0 1779 2301 Size: 1779 522 PfoI T'CCnGG_A Cuts at: 0 95 550 812 1631 2301 Size: 95 455 262 819 670 Fragments arranged by size: 819 670 455 262 95 PmlI CAC'GTG Cuts at: 0 1761 2301 Size: 1761 540 PpuMI rG'GwC_Cy Cuts at: 0 83 243 1629 2301 Size: 83 160 1386 672 Fragments arranged by size: 1386 672 160 83 PspOMI G'GGCC_C Cuts at: 0 1895 2262 2301 Size: 1895 367 39 Fragments arranged by size: 1895 367 39 PstI C_TGCA'G Cuts at: 0 158 836 1370 1808 2104 2301 Size: 158 678 534 438 296 197 Fragments arranged by size: 678 534 438 296 197 158 PvuII CAG'CTG Cuts at: 0 966 1980 2301 Size: 966 1014 321 Fragments arranged by size: 1014 966 321 SacI G_AGCT'C Cuts at: 0 307 355 2042 2301 Size: 307 48 1687 259 Fragments arranged by size: 1687 307 259 48 SacII CC_GC'GG Cuts at: 0 607 750 1293 2165 2301 Size: 607 143 543 872 136 Fragments arranged by size: 872 607 543 143 136 SalI G'TCGA_C Cuts at: 0 561 2301 Size: 561 1740 SanDI GG'GwC_CC Cuts at: 0 83 243 2301 Size: 83 160 2058 Fragments arranged by size: 2058 160 83 SapI GCTCTTCn'nnn_ Cuts at: 0 1819 2301 Size: 1819 482 ScaI AGT'ACT Cuts at: 0 1384 2301 Size: 1384 917 SexAI A'CCwGG_T Cuts at: 0 1604 1778 2301 Size: 1604 174 523 Fragments arranged by size: 1604 523 174 SfcI C'TryA_G Cuts at: 0 154 832 1366 1804 2100 2301 Size: 154 678 534 438 296 201 Fragments arranged by size: 678 534 438 296 201 154 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1894 1945 2301 Size: 1894 51 356 Fragments arranged by size: 1894 356 51 SfoI GGC'GCC Cuts at: 0 1613 2301 Size: 1613 688 SmaI CCC'GGG Cuts at: 0 1702 2301 Size: 1702 599 SphI G_CATG'C Cuts at: 0 456 1116 2301 Size: 456 660 1185 Fragments arranged by size: 1185 660 456 StuI AGG'CCT Cuts at: 0 949 1888 2301 Size: 949 939 413 Fragments arranged by size: 949 939 413 StyI C'CwwG_G Cuts at: 0 1590 2301 Size: 1590 711 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 191 2301 Size: 191 2110 TatI w'GTAC_w Cuts at: 0 194 992 1175 1241 1382 1565 2301 Size: 194 798 183 66 141 183 736 Fragments arranged by size: 798 736 194 183 183 141 66 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 358 390 904 936 2301 Size: 358 32 514 32 1365 Fragments arranged by size: 1365 514 358 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1474 2301 Size: 1474 827 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 231 963 2163 2301 Size: 231 732 1200 138 Fragments arranged by size: 1200 732 231 138 XmaI C'CCGG_G Cuts at: 0 1700 2301 Size: 1700 601 ZraI GAC'GTC Cuts at: 0 1965 2301 Size: 1965 336 Enzymes that do cut: AatII AccI AcuI AfeI AflIII AhdI AlfI AlwNI ApaI ApaLI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BfrBI BglI Bme1580I BmgBI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BssSI BstEII BstYI Bsu36I BtgI BtsI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SacI SacII SalI SanDI SapI ScaI SexAI SfcI SfiI SfoI SmaI SphI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI ZraI Enzymes that do not cut: AarI Acc65I AclI AflII AgeI AleI AloI ApoI AscI AseI AsiSI AvrII BbvCI BciVI BclI BglII BlpI BmrI BmtI BpuEI BsaBI BsiEI BsiWI BsmI BspHI BsrDI BssHII BstAPI BstBI BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII EagI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI NcoI NdeI NheI NotI NruI PacI PasI PciI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SbfI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI SwaI XbaI XhoI XmnI