################################## # NO CUT ENZYMES ( 194 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BcnI BfrI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HapII HgaI Hin1I HincII HindII HpaI HpaII KasI KpnI Ksp632I KspI MaeII MaeIII MamI MflI MluI MroI MslI MspA1I MspI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfcI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I TspRI Tth111I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AcsI 477 GAATTT [GA]^AATT[CT] ApoI 477 GAATTT [GA]^AATT[CT] BalI 182 TGGCCA TGG^CCA BbvI 127 GCTGC GCAGC(8/12) BciVI 447 GTATCC GTATCC(6/5) BclI 384 TGATCA T^GATCA BfaI 83 CTAG C^TAG BglI 175 GCCAATGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 117 CTCCAG CTGGAG(16/14) BsaI 106 GAGACC GGTCTC(1/5) BsiYI 29 CCATTGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 29 CCATTGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsrDI 78 CATTGC GCAATG(2/0) Cac8I 190 GCTGGC GC[ACGT]^[ACGT]GC CfoI 226 GCGC GCG^C EaeI 182 TGGCCA [CT]^GGCC[GA] EcoT14I 185 CCAAGG C^C[AT][AT]GG FbaI 384 TGATCA T^GATCA Fnu4HI 127 GCTGC GC^[ACGT]GC HhaI 226 GCGC GCG^C HinP1I 226 GCGC G^CGC HindIII 413 AAGCTT A^AGCTT HinfI 141 GAATC G^A[ACGT]TC ItaI 127 GCTGC GC^[ACGT]GC MaeI 83 CTAG C^TAG MfeI 470 CAATTG C^AATTG MluNI 182 TGGCCA TGG^CCA MscI 182 TGGCCA TGG^CCA MunI 470 CAATTG C^AATTG MwoI 175 GCCAATGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIV 234 GGTTCC GG[ACGT]^[ACGT]CC PflMI 29 CCATTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SfaNI 271 GATGC GCATC(5/9) SfiI 174 GGCCAATGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC StyI 185 CCAAGG C^C[AT][AT]GG TaqI 155 TCGA T^CGA TfiI 141 GAATC G^A[AT]TC TseI 127 GCTGC G^C[AT]GC TthHB8I 155 TCGA T^CGA Van91I 29 CCATTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### BsaJI 185 CCAAGG C^C[ACGT][ACGT]GG 263 CCCAGG BsmAI 106 GAGAC GTCTC(1/5) 315 GTCTC BsrI 35 ACTGG ACTGG(1/-1) 158 ACTGG BstF5I 201 GGATG GGATG(2/0) 270 GGATG DdeI 137 CTGAG C^T[ACGT]AG 305 CTGAG DpnII 54 GATC ^GATC 385 GATC FokI 201 GGATG GGATG(9/13) 270 GGATG HphI 113 TCACC GGTGA(8/7) 462 TCACC MboI 54 GATC ^GATC 385 GATC MseI 221 TTAA T^TAA 373 TTAA NdeII 54 GATC ^GATC 385 GATC Sau3AI 54 GATC ^GATC 385 GATC Tru9I 221 TTAA T^TAA 373 TTAA XcmI 148 CCAGCTATCGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 185 CCAAGGCTGGCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 10 enzymes) # ######################################### AluI BssKI BstNI EcoRII HaeIII MboII MnlI MvaI ScrFI Tsp509I